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1. 发明公开

EP0830363A1 CHEMICAL LIGATION OF TEMPLATE-DIRECTED OLIGONUCLEOTIDES 失效
标题翻译：模板寡核苷酸的化学结合
公开(公告)号：EP0830363A1
公开(公告)日：1998-03-25
申请号：EP96915480.0
申请日：1996-05-03
申请人： NORTHWESTERN UNIVERSITY
发明人： LETSINGER, Robert, L. , GRYAZNOV, Serge M.
IPC分类号： C07H21 , C12Q1
CPC分类号： C07H21/00 , C12Q1/6813 , C12Q1/6827 , C12Q2523/101 , C12Q2523/109 , C12Q2561/125 , C12Q2565/401
摘要： A method of increasing oligonucleotide selectivity in recognition of nucleic acids is disclosed, the method including the steps of disposing in aqueous solution a first oligomer having high selectivity for a target polynucleotide and a second oligomer having high affinity for an adjacent segment of the target polynucleotide. The oligomers include first and second reactive groups capable of spontaneously and irreversibly forming stable covalent bonds between the oligomers in absence of added chemical reagents or enzymes. A method for hybridizing nucleic acids includes the steps of reversibly binding a first oligomer to a target oligo- or polynucleotide including base units complementary to base units of the oligonucleotide, reversibly binding a second oligomer to the target oligo- or polynucleotide including base units complementary to base units of the oligonucleotide adjacent to the first oligomer, and wherein one of the oligomers includes a nucleotide having a first reactive group proximate to a nucleotide of the other oligomer which includes a second reactive group capable of spontaneously forming a covalent bond with the first reactive group. The method further includes the steps of irreversibly covalently joining the oligomers together through the first and the second reactive groups having been brought in proximity to each other upon binding of the oligonucleotides on the target polynucleotide in the absence of added reagent or enzyme, dissociating the joined first and second oligomers from the target polynucleotide, and removing the target polynucleotide.
摘要翻译：公开了增加识别核酸的寡核苷酸选择性的方法，所述方法包括以下步骤：将对靶多核苷酸具有高选择性的第一寡聚物和对靶多核苷酸的相邻区段具有高亲和力的第二寡聚物置于水溶液中。低聚物包括第一和第二反应性基团，其能够在不添加化学试剂或酶的情况下自发地和不可逆地在低聚物之间形成稳定的共价键。用于杂交核酸的方法包括以下步骤：将第一寡聚物可逆结合至包含与寡核苷酸的碱基单元互补的碱基单元的目标寡聚核苷酸或多聚核苷酸，将第二寡聚物可逆地结合至目标寡聚核苷酸或多聚核苷酸，寡核苷酸的与第一寡聚物相邻的碱基单元，并且其中一个寡聚物包括具有第一反应基团的核苷酸，所述第一反应基团邻近另一寡聚物的核苷酸，所述另一寡聚物包含能够与第一反应物自发形成共价键的第二反应基团组。该方法进一步包括以下步骤：通过第一和第二反应性基团将寡聚体不可逆地共价连接在一起，所述第一和第二反应性基团在寡核苷酸在不添加试剂或酶的情况下与靶多核苷酸结合后彼此接近，分离接合体来自靶多核苷酸的第一和第二寡聚物，并去除靶多核苷酸。

2. 发明公开

EP0428707A1 STEROID MODIFIED OLIGONUCLEOTIDES 失效
标题翻译：类固醇修饰的寡核苷酸。
公开(公告)号：EP0428707A1
公开(公告)日：1991-05-29
申请号：EP90909872.0
申请日：1990-06-06
申请人： NORTHWESTERN UNIVERSITY
发明人： LETSINGER, Robert, L.
IPC分类号： A61K31 , A61P31 , C07H21 , C07J43 , C07J51
CPC分类号： C07H21/00 , A61K47/542 , A61K47/554 , C07J51/00
摘要： L'invention concerne des oligonucléotides modifiés au niveau de leurs squelettes par la fixation d'au moins un stéroïde. Les oligonucléotides modifiés s'ancrent dans la membrane cellulaire afin de servir de sonde et d'exercer une activité thérapeutique.

3. 发明公开

EP1360318A2 METHOD OF DETECTION BY ENHANCEMENT OF SILVER STAINING 有权
标题翻译：检测方法由增加的银色
公开(公告)号：EP1360318A2
公开(公告)日：2003-11-12
申请号：EP01954641.5
申请日：2001-07-11
申请人： NORTHWESTERN UNIVERSITY
发明人： LETSINGER, Robert, L. , GARIMELLA, Viswanadham
IPC分类号： C12Q1/68
CPC分类号： B82Y15/00 , C12Q2563/137 , G01N27/44721 , G01N27/44726 , Y10S977/924
摘要： The present invention relates to a method for amplifying a detection signal by enhancing or promoting the deposition of additional silver in assay detection systems where the formation of a silver spot serves as a reporter for the presence of a target molecule, including biological polymers (e.g., proteins and nucleic acids) and small molecules.

4. 发明授权

EP1360318B1 METHOD OF DETECTION BY ENHANCEMENT OF SILVER STAINING 有权
标题翻译：检测方法由增加的银色
公开(公告)号：EP1360318B1
公开(公告)日：2006-04-26
申请号：EP01954641.5
申请日：2001-07-11
申请人： Northwestern University
发明人： LETSINGER, Robert, L. , GARIMELLA, Viswanadham
IPC分类号： C12Q1/68
CPC分类号： B82Y15/00 , C12Q2563/137 , G01N27/44721 , G01N27/44726 , Y10S977/924
摘要： The present invention relates to a method for amplifying a detection signal by enhancing or promoting the deposition of additional silver in assay detection systems where the formation of a silver spot serves as a reporter for the presence of a target molecule, including biological polymers (e.g., proteins and nucleic acids) and small molecules.

5. 发明授权

EP0695305B1 METHOD OF FORMING OLIGONUCLEOTIDES 失效
标题翻译：法制备寡核苷酸作者：
公开(公告)号：EP0695305B1
公开(公告)日：2003-08-06
申请号：EP94914046.1
申请日：1994-04-06
申请人： NORTHWESTERN UNIVERSITY
发明人： LETSINGER, Robert, L. , GRYAZNOV, Sergei, M.
IPC分类号： C07H21/00 , C07H21/04
CPC分类号： C07H21/00 , C12Q1/6813 , C12Q2523/109
摘要： A method of forming an oligonucleotide is disclosed, the method including the steps of disposing in solution at least two oligonucleotides in aqueous solution wherein one of the oligonucleotides includes an α-haloacyl group and the other of the nucleotides includes a phosphothioate group and covalently binding the oligonucleotides together through the α-haloacyl group and the phosphothioate group spontaneously forming a thiophosphorylacetylamino group therebetween.

6. 发明公开

EP0785942A1 CATIONIC OLIGONUCLEOTIDES, AND RELATED METHODS OF SYNTHESIS AND USE 失效
标题翻译：阳离子寡方法生产和使用Darvon
公开(公告)号：EP0785942A1
公开(公告)日：1997-07-30
申请号：EP96929675.0
申请日：1996-08-01
申请人： CHIRON CORPORATION , NORTHWESTERN UNIVERSITY
发明人： HORN, Thomas , LETSINGER, Robert, L. , BALASUBRAMANIAM, Tanjore, Narayanaswamy
IPC分类号： C12N15 , C07H21 , C12Q1
CPC分类号： C07H21/00 , C12Q1/68 , C12Q1/6839
摘要： Novel oligonucleotides are provided having cationic internucleoside linkages. The cationic internucleoside linkage has structure (I), wherein W, X, Y, Z, R?1, R2 and R3¿ are as defined herein, P* represents an asymetric phosphorus atom capable of existing in two distinct stereoisomeric configurations, and further wherein the internucleoside linkage is stereouniform. Certain of these oligonucleotides may have alternating anionic and cationic internucleoside linkages, which are not necessarily stereouniform. A method for synthesizing the compounds are provided as well, as are methods for using the compounds, e.g., as antisense molecules and in nucleic acid hybridization assays.

7. 发明公开

EP0695305A1 METHOD OF FORMING OLIGONUCLEOTIDES 失效
标题翻译：法制备寡核苷酸作者：
公开(公告)号：EP0695305A1
公开(公告)日：1996-02-07
申请号：EP94914046.0
申请日：1994-04-06
申请人： NORTHWESTERN UNIVERSITY
发明人： LETSINGER, Robert, L. , GRYAZNOV, Sergei, M.
IPC分类号： C12N15 , A61K31 , A61K48 , A61P31 , A61P35 , A61P43 , C07H21 , C12Q1
CPC分类号： C07H21/00 , C12Q1/6813 , C12Q2523/109
摘要： A method of forming an oligonucleotide is disclosed, the method including the steps of disposing in solution at least two oligonucleotides in aqueous solution wherein one of the oligonucleotides includes an α-haloacyl group and the other of the nucleotides includes a phosphothioate group and covalently binding the oligonucleotides together through the α-haloacyl group and the phosphothioate group spontaneously forming a thiophosphorylacetylamino group therebetween.

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