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1. 发明授权

EP2495564B1 5.9 kDa PEPTIDE IMMUNOASSAY METHOD 有权
标题翻译： 5.9-KDA肽免疫测定法规程
公开(公告)号：EP2495564B1
公开(公告)日：2015-12-16
申请号：EP10826514.1
申请日：2010-10-13
申请人： Nitto Boseki Co., Ltd
发明人： KIKUCHI Wataru , SATO Momoe , NODA Kenta , KIYOKAWA Iwao , MIURA Toshihide , KOJIMA Ryo , NOMURA Fumio , SOGAWA Kazuyuki
IPC分类号： G01N33/53 , C07K16/18
CPC分类号： G01N33/6893 , C07K16/36 , C07K2317/34 , G01N33/5308 , G01N2333/75 , G01N2800/08

2. 发明授权

EP2309265B1 METHOD OF ASSAYING COMPLEX AND KIT TO BE USED THEREFOR 有权
标题翻译：的程序对IT复杂而KIT;适用
公开(公告)号：EP2309265B1
公开(公告)日：2013-07-17
申请号：EP09802770.9
申请日：2009-05-14
申请人： Nitto Boseki CO., LTD. , Shinshu University
发明人： FUJITA Kiyotaka , KOJIMA Ryo , SATO Yoshiro , SATO Natsuki
IPC分类号： G01N33/543 , G01N21/82 , G01N33/53 , G01N33/545 , G01N33/536
CPC分类号： G01N33/536 , G01N2333/765

3. 发明公开

EP2530465A1 METHOD FOR IMMUNOASSAY OF AUTOANTIBODY AGAINST KU86, KIT FOR USE IN SAME, AND METHOD FOR DETERMINATION OF PRIMARY HEPATOCELLULAR CARCINOMA USING SAME 有权
标题翻译：法与自身抗体抗Ku86，KIT在其中使用和方法免疫法厘定原始血液细胞肿瘤组织中的
公开(公告)号：EP2530465A1
公开(公告)日：2012-12-05
申请号：EP11734623.9
申请日：2011-01-18
申请人： Nitto Boseki Co., Ltd
发明人： KOJIMA Ryo , NODA Kenta , SEIMIYA Masanori , SOGAWA Kazuyuki , NOMURA Fumio
IPC分类号： G01N33/53 , G01N33/533 , G01N33/534 , G01N33/535 , G01N33/574
CPC分类号： G01N33/6854 , G01N33/564 , G01N33/57438 , G01N33/57484 , G01N2333/47 , G01N2333/90
摘要： An autoantibody against Ku86 can be measured by reacting the autoantibody contained in a sample with a Ku86 antigen (which serves as a reagent) to produce an immune complex of the autoantibody and the Ku86 antigen and measuring the immune complex using a labeled anti-human immunoglobulin antibody. The measurement of the autoantibody enables the determination of primary hepatocellular carcinoma.

4. 发明公开

EP2309265A1 METHOD OF ASSAYING COMPLEX AND KIT TO BE USED THEREFOR 有权
标题翻译： VERFAHREN ZUR UNTERSUCHUNG EINES KOMPLEXES UND DARIN ZU VERWENDENDES KIT
公开(公告)号：EP2309265A1
公开(公告)日：2011-04-13
申请号：EP09802770.9
申请日：2009-05-14
申请人： Nitto Boseki CO., LTD. , Shinshu University
发明人： FUJITA Kiyotaka , KOJIMA Ryo , SATO Yoshiro , SATO Natsuki
IPC分类号： G01N33/543 , G01N21/82 , G01N33/53 , G01N33/545
CPC分类号： G01N33/536 , G01N2333/765
摘要： In the case of assaying the level of complex AB in a sample which likely contains the complex AB composed of substance A with another substance B, the complex is assayed by the competitive homogeneous assay method with the use of reagents including a reagent containing partner C specifically binding to substance A, a reagent containing partner D specifically binding to substance B, a reagent containing fine particles carrying substance A or an analogue thereof and substance B or an analogue thereof, and a reagent containing partner C specifically binding to substance A and partner D specifically binding to substance B. Thus, the complex in the sample can be easily assayed. The above method is applicable to general-purpose biochemical automatic analyzers.
摘要翻译：在可能含有由物质A与其他物质B组成的复合物AB的样品中测定复合物AB的水平的情况下，通过竞争性均一测定方法使用试剂（包括含有对象C的试剂）与物质A结合，与物质B特异性结合的含有配子D的试剂，含有携带物质A的微粒或其类似物的试剂或其物质B或其类似物，以及与物质A和配偶体D特异性结合的含有试剂的配偶体C 特异性地结合物质B.因此，可以容易地测定样品中的复合物。上述方法适用于通用生化自动分析仪。

5. 发明公开

EP2495564A1 5.9 kDa PEPTIDE IMMUNOASSAY METHOD 有权
标题翻译： IMMUNOASSAY-VERFAHREN MIT 5,9-KDA-PEPTID
公开(公告)号：EP2495564A1
公开(公告)日：2012-09-05
申请号：EP10826514.1
申请日：2010-10-13
申请人： Nitto Boseki Co., Ltd
发明人： KIKUCHI Wataru , SATO Momoe , NODA Kenta , KIYOKAWA Iwao , MIURA Toshihide , KOJIMA Ryo , NOMURA Fumio , SOGAWA Kazuyuki
IPC分类号： G01N33/53 , C07K16/18
CPC分类号： G01N33/6893 , C07K16/36 , C07K2317/34 , G01N33/5308 , G01N2333/75 , G01N2800/08
摘要： Disclosed is an immunoassay method whereby a 5.9kDa peptide which results from the degradation of the α-E chain and α chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.
摘要翻译：公开了一种免疫测定方法，其中可以在含有污染肽的生物样品中特异性地测定了由人类纤维蛋白原的±-E链和±链的降解引起的并用作诊断肝脏疾病的肽标记物的5.9kDa肽通过将识别所述肽标记的N末端的抗体和识别所述肽标记的C末端的抗体与所述肽标记接触，形成所述肽标记和两种抗体的免疫复合物，并免疫测定所获得的免疫复合物。

6. 发明公开

EP2460888A1 METHOD AND KIT FOR MEASUREMENT OF DEHYDROGENASE OR SUBSTRATE FOR THE DEHYDROGENASE 有权
标题翻译：方法和试剂盒用于测量脱氢酶或基底脱氢酶的
公开(公告)号：EP2460888A1
公开(公告)日：2012-06-06
申请号：EP10804211.0
申请日：2010-06-21
申请人： Nitto Boseki CO., LTD.
发明人： NODA Kenta , SATO Yoshiro , KOJIMA Ryo
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32 , C12Q1/54 , C12Q1/58
摘要： Disclosed is a method for measuring a dehydrogenase or a substrate for the dehydrogenase contained in a sample, wherein both thio-NAD or thio-NADP and NAD or NADP are used as coenzymes. The method enables the measurement on the basis of absorbance values obtained in a visible region, and also enables the accurate measurement of a dehydrogenase or a substrate for the dehydrogenase which is contained in a sample at a high concentration.

7. 发明授权

EP2460888B1 METHOD AND KIT FOR MEASUREMENT OF DEHYDROGENASE OR SUBSTRATE FOR THE DEHYDROGENASE 有权
标题翻译：用于测定脱氢酶或脱氢酶的底物的方法和试剂盒
公开(公告)号：EP2460888B1
公开(公告)日：2018-05-09
申请号：EP10804211.0
申请日：2010-06-21
申请人： Nitto Boseki CO., LTD.
发明人： NODA Kenta , SATO Yoshiro , KOJIMA Ryo
IPC分类号： C12Q1/32 , C12Q1/54 , C12Q1/58
CPC分类号： C12Q1/32 , C12Q1/54 , C12Q1/58
摘要： Disclosed is a method for measuring a dehydrogenase or a substrate for the dehydrogenase contained in a sample, wherein both thio-NAD or thio-NADP and NAD or NADP are used as coenzymes. The method enables the measurement on the basis of absorbance values obtained in a visible region, and also enables the accurate measurement of a dehydrogenase or a substrate for the dehydrogenase which is contained in a sample at a high concentration.

8. 发明授权

EP2581446B1 METHOD FOR PRODUCING PENTAMERIC CRP, PENTAMERIC CRP-PRODUCING TRANSGENIC SILKWORM AND METHOD FOR CONSTRUCTING SAME, DNA ENCODING CANINE MONOMERIC CRP AND EXPRESSION VECTOR CONTAINING THE DNA 有权转让
标题翻译：用于生产五聚CRP，转基因蚕生产用于FOR A犬单体CRP及表达载体与DNA产生与DNA编码的五聚体CRP AND PROCESS
公开(公告)号：EP2581446B1
公开(公告)日：2017-02-22
申请号：EP11792315.1
申请日：2011-05-30
申请人： Nitto Boseki Co., Ltd , National Agriculture and Food Research Organization
发明人： KIYOKAWA Iwao , ARIMATSU Yuji , MIURA Toshihide , KOJIMA Ryo , SEZUTSU Hideki , UCHINO Keiro , KOBAYASHI Isao , TAMURA Toshiki
IPC分类号： C12N15/09 , A01K67/033 , A01K67/04 , C12P21/02 , C12N15/85
CPC分类号： C12N15/8509 , A01K67/033 , A01K67/0335 , A01K67/04 , A01K2217/052 , A01K2227/706 , A01K2267/01 , C12N15/09 , C12P21/02

9. 发明授权

EP2530465B1 METHOD FOR IMMUNOASSAY OF AUTOANTIBODY AGAINST KU86, KIT FOR USE IN SAME, AND METHOD FOR DETERMINATION OF PRIMARY HEPATOCELLULAR CARCINOMA USING SAME 有权
标题翻译：法与自身抗体抗Ku86，KIT在其中使用和方法免疫法厘定原始血液细胞肿瘤组织中的
公开(公告)号：EP2530465B1
公开(公告)日：2015-07-29
申请号：EP11734623.9
申请日：2011-01-18
申请人： Nitto Boseki Co., Ltd
发明人： KOJIMA Ryo , NODA Kenta , SEIMIYA Masanori , SOGAWA Kazuyuki , NOMURA Fumio
IPC分类号： G01N33/53 , G01N33/533 , G01N33/534 , G01N33/535 , G01N33/574
CPC分类号： G01N33/6854 , G01N33/564 , G01N33/57438 , G01N33/57484 , G01N2333/47 , G01N2333/90

10. 发明公开

EP2535715A1 METHOD FOR MEASURING IMMUNITY OF COMPLEX OF Ku86 AND AUTOANTIBODY THEREOF, KIT USED THEREFOR, AND METHOD FOR DETERMINING CANCER USING SAME 有权转让
标题翻译：法测量Ku86配合物和自身抗体免疫力其从未使用试剂盒和方法用于癌症诊断，以便
公开(公告)号：EP2535715A1
公开(公告)日：2012-12-19
申请号：EP11742179.2
申请日：2011-02-07
申请人： Nitto Boseki Co., Ltd
发明人： KOJIMA Ryo , NODA Kenta , SEIMIYA Masanori , SOGAWA Kazuyuki , NOMURA Fumio
IPC分类号： G01N33/574 , G01N33/53
CPC分类号： G01N33/57484 , G01N33/564 , G01N2333/922
摘要： A reaginic antibody of Ku86 and a substance capable of binding with the autoantibody of Ku86 are acted on a complex of Ku86 and the autoantibody thereof in a subject. Said complex can be measured by measuring the obtained immune complex of the aforementioned complex, reaginic antibody, and substance capable of binding with the autoantibody of Ku86. As a consequence, it is possible to determine whether the cancer is a primary hepatoma, colon cancer, stomach cancer, pancreatic cancer, breast cancer, lung cancer, or an esophageal cancer.
摘要翻译： Ku86的reaginic抗体和能够与Ku86的自身抗体结合的物质被作用于Ku86的复杂和在受试者中自身抗体物。所述复合物可以通过测量获得上述复合物，reaginic抗体，和物质能够与Ku86的自身抗体结合的免疫复合物进行测定。因此，有可能确定矿是否癌症是原发性肝癌，结肠癌，胃癌，胰腺癌，乳腺癌，肺癌，或食道癌。

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