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    • 4. 发明公开
    • CIRCULAR DICHROISM FLUORESCENT MICROSCOPE
    • ZIRKULARDICHROISMUS-FLUORESZENZMIKROSKOP
    • EP1980842A1
    • 2008-10-15
    • EP07713762.8
    • 2007-02-01
    • National University Corporation Nara Institute of Science and Technology
    • KAWAI, TsuyoshiKAWAMURA, Kensuke
    • G01N21/64G02B21/00
    • G01N21/19G01N21/6445G01N21/6458G02B21/16
    • According to a circular dichroism fluorescent microscope 100, it is possible to analyze, by using a small amount of a sample and directly in a living organism, a high-order structure of the sample such as a biomolecule, for example, protein and the like. The circular dichroism fluorescent microscope 100 includes a confocal section (115). In the circular dichroism fluorescent microscope 100, a circularly polarizing/modulating section (105) converts, into right and left circularly polarized lights, a light beam emitted from a light source (103). Thus obtained right and left circularly polarized light is focused on the sample so that the sample is irradiated with the right and left circularly polarized lights. Then, an optical lens (102) focuses fluorescence emitted from the sample. Further, a wavelength selecting section (107) transmits only fluorescence having a predetermined wavelength. Subsequently, the fluorescence having passed through the wavelength selecting section 107 is detected. Based on fluorescent intensity signals of the fluorescence thus detected, a difference between (i) an intensity of the fluorescence emitted from the sample at the time of irradiation with the use of the right circularly polarized light and (ii) an intensity of the fluorescence emitted from the sample at the time of irradiation with the use of the left circularly polarized light is calculated.
    • 根据圆形二色性荧光显微镜100,可以通过使用少量样品并直接在活体中分析生物分子例如蛋白质等样品的高阶结构 。 圆二色性荧光显微镜100包括共焦部(115)。 在圆二色性荧光显微镜100中,圆偏振/调制部(105)将从光源(103)射出的光束转换为右,左圆偏振光。 这样获得的右和左圆偏振光聚焦在样品上,以便用右和左圆偏振光照射样品。 然后,光学透镜(102)聚焦从样品发出的荧光。 此外,波长选择部(107)仅发送具有预定波长的荧光。 随后,检测通过波长选择部分107的荧光。 基于如此检测的荧光的荧光强度信号,(i)使用右圆偏振光从照射时的样品发射的荧光的强度和(ii)发射的荧光的强度之间的差异 计算使用左圆偏振光的照射时的样品。