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1. 发明公开

EP3260862A2 METHOD FOR SUPPRESSING PROTEIN ADSORPTION 有权
标题翻译：抑制蛋白质吸附的方法
公开(公告)号：EP3260862A2
公开(公告)日：2017-12-27
申请号：EP16752538.5
申请日：2016-02-18
申请人： National University Corporation Kyoto Institute of Technology , NOF Corporation
发明人： KUMADA, Yoichi , KAWAGUCHI, Yasutaka , NEMOTO, Aranna , NAKASHIMA, Fumio , SAKAMOTO, Nobuyuki
IPC分类号： G01N33/48
CPC分类号： G01N33/48 , C07F9/4461 , C08F220/56 , C08F222/385
摘要： Provided is a novel method of suppressing non-specific protein adsorption. The above-mentioned object can be achieved by treating a solid phase with a solid phase-treating liquid containing a phosphorylcholine group-containing polymer as a main component, and treating a specimen with a specimen-treating liquid containing, as a main component, a polymer having a phosphorylcholine group, a hydroxy group, and a hydrophobic group, followed by addition of the treated specimen to the treated solid phase.
摘要翻译：提供了一种抑制非特异性蛋白质吸附的新方法。上述目的可以通过用含有含磷酸胆碱基团的聚合物作为主要成分的固相处理液处理固相，并用含有作为主要组分的试样处理液处理试样来实现，具有磷酸胆碱基团，羟基和疏水基团的聚合物，随后将处理过的试样加入到处理过的固相中。

2. 发明公开

EP2816110A1 PEPTIDE HAVING AFFINITY FOR SILICON NITRIDE (SI3N4), AND USE THEREFOR 有权
标题翻译： PEPTIDE MITAFFINITÄTFÜRSILICIUMNITRID（SI3N4）UND VERWENDUNG DAVON
公开(公告)号：EP2816110A1
公开(公告)日：2014-12-24
申请号：EP13749185.8
申请日：2013-02-12
申请人： National University Corporation Kyoto Institute of Technology
发明人： KUMADA, Yoichi , KISHIMOTO, Michimasa , OTSUKA, Takeru
IPC分类号： C12N15/09 , C07K14/245 , C07K17/14 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N11/14
CPC分类号： C07K17/14 , C07K7/06 , C07K7/08 , C07K14/245 , C07K2319/00 , C07K2319/23 , C12N9/0006 , C12N11/14
摘要： The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride. The invention involves a peptide having an affinity for silicon nitride, the peptide comprising (1-1) a peptide having the amino acid sequence of one of SEQ ID NOS: 1, 2 and 23 to 27, (1-2) a peptide that has an affinity for silicon nitride and comprises an amino acid sequence obtained by deleting, adding, and/or substituting one or more amino acids in one of the amino acid sequences indicated in (1-1), or a fragment of one of the peptides.
摘要翻译：本发明的目的是提供：对氮化硅具有亲和性的肽; 编码该肽的多核苷酸; 用于表达对氮化硅具有亲和性的肽的表达载体; 用于表达包含对氮化硅和靶蛋白具有亲和力的肽的肽融合蛋白的表达载体; 通过将表达载体导入宿主细胞获得的转化体; 从转化体获得的肽融合蛋白; 已经结合了对氮化硅具有亲和性的肽的氮化硅衬底; 用于将靶蛋白固定到氮化硅衬底的方法; 用于将靶蛋白固定到氮化硅衬底的组合物，所述组合物包含对氮化硅具有亲和性的肽; 以及用于将靶蛋白固定到氮化硅衬底的接头，所述接头包含对氮化硅具有亲和性的肽。本发明涉及对氮化硅具有亲和力的肽，所述肽包含（1-1）具有SEQ ID NO：1,2和23至27之一的氨基酸序列的肽，（1-2）肽，对氮化硅具有亲和性，并且包含通过在（1-1）中指出的氨基酸序列之一中缺失，添加和/或取代一个或多个氨基酸而获得的氨基酸序列，或其中一个肽的一个片段。

3. 发明公开

EP2957633A1 METHOD FOR REFOLDING ANTIBODY, PROCESS FOR PRODUCING REFOLDED ANTIBODY, REFOLDED ANTIBODY, AND USES THEREOF 无效
标题翻译：方法复性的抗体，产生方法折返抗体BACK折叠的抗体及其用途
公开(公告)号：EP2957633A1
公开(公告)日：2015-12-23
申请号：EP14752135.5
申请日：2014-02-03
申请人： National University Corporation Kyoto Institute of Technology
发明人： KUMADA, Yoichi , ISHIKAWA, Yasuyuki , FUJIWARA, Yusuke , KISHIMOTO, Michimasa
IPC分类号： C12N15/09 , C07K16/18 , C07K17/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10
CPC分类号： C07K16/4241 , C07K16/065 , C07K16/18 , C07K16/26 , C07K16/3007 , C07K16/40 , C07K17/08 , C07K2317/14 , C07K2317/40 , C07K2317/622 , C07K2319/35 , C08F122/10
摘要： The present invention provides a method for refolding an antibody, a process for producing a refolded antibody, a refolded antibody, and uses thereof. The present invention provides a method for refolding an antibody in a liquid phase, the method comprising the steps of denaturing an inactive antibody binding directly or through a linker to a peptide, the peptide having an isoelectric point lower than the isoelectric point of the inactive antibody, and dispersing in a liquid phase the peptide-binding inactive antibody denatured in the step above. The present invention also provides a process for producing a refolded antibody.
摘要翻译：本发明提供了一种抗体，用于产生重折叠抗体，重折叠抗体的过程的重折叠的方法，及其用途。本发明提供了一种用于在液相中抗体的重折叠，包括无活性的抗体直接或通过一个左到肽结合的变性步骤的方法中，具有在等电点比非活性抗体的等电点低的肽和在液相中在上述步骤中变性肽结合非活性抗体分散。因此，本发明提供了一种生产重折叠抗体的方法。

4. 发明公开

EP3816189A1 SEPARATING AGENT 审中-公开
公开(公告)号：EP3816189A1
公开(公告)日：2021-05-05
申请号：EP19825288.4
申请日：2019-07-01
申请人： National University Corporation Kyoto Institute of Technology , Daicel Corporation
发明人： KUMADA, Yoichi , OKURA, Hiromichi , UCHIMURA, Seiichi
IPC分类号： C07K17/00 , C07K16/42 , G01N33/50 , C12N15/13
摘要： An object of the present invention is to provide a separating agent that does not have a significantly reduced dynamic binding capacity (DBC) to a target substance even when the separating agent is continually CIP-treated under alkaline pH conditions, and the object is achieved with a separating agent including a carrier and a protein, wherein the protein is a given protein, and a surface of the carrier and the lysine residue in the protein are bound by a chemical bond.

5. 发明公开

EP3375875A1 SEPARATING AGENT FOR HUMAN SERUM-DERIVED IgG POLYCLONAL ANTIBODIES, AND METHOD FOR SEPARATING HUMAN SERUM-DERIVED IgG POLYCLONAL ANTIBODIES USING SAME 审中-公开
公开(公告)号：EP3375875A1
公开(公告)日：2018-09-19
申请号：EP16864174.4
申请日：2016-11-07
申请人： National University Corporation Kyoto Institute of Technology , Daicel Corporation
发明人： KUMADA, Yoichi , HASEGAWA, Yuya , UCHIMURA, Seiichi
IPC分类号： C12N15/09 , C07K1/22 , C07K16/42 , C12P21/08 , C12Q1/70
CPC分类号： C12P21/005 , C07K1/22 , C07K16/065 , C07K16/42 , C07K2317/14 , C07K2317/92 , C12N15/09 , C12N15/62 , C12Q1/70
摘要： An object of the present invention is to provide a separation agent for separating a human serum-derived IgG polyclonal antibody. This object is achieved by a separation agent for separating a human serum-derived IgG polyclonal antibody, the separation agent including: a carrier; and a single-chain antibody which has a dissociation constant for a human serum-derived lgG polyclonal antibody of not more than 3.0 × 10 -8 M and which binds to the surface of the carrier via a chemical bond.

6. 发明公开

EP3257868A1 PEPTIDES HAVING AFFINITY FOR POLYDIMETHYLSILOXANE, AND USES THEREOF 审中-公开
标题翻译：对聚二甲基硅氧烷具有亲和性的肽及其用途
公开(公告)号：EP3257868A1
公开(公告)日：2017-12-20
申请号：EP16749338.6
申请日：2016-02-12
申请人： National University Corporation Kyoto Institute of Technology , Nissan Chemical Industries, Ltd.
发明人： KUMADA, Yoichi , OTSUKI, Ryoko , AKAI, Ryota , KATAYAMA, Junko , MATOBA, Kazutaka
IPC分类号： C07K17/08 , C07K14/00 , C12N7/08 , C12N9/00 , C12N15/00
CPC分类号： C07K17/08 , C07K14/00 , C08G77/04 , C12N7/08 , C12N9/00 , C12N15/00 , C12N15/63
摘要： An object of the present invention is to provide a polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane (PDMS) is bound; a method for immobilizing a target protein on a polydimethylsiloxane substrate; a peptide having an affinity for PDMS; a polynucleotide encoding a peptide having an affinity for PDMS; and a vector using thereof. A polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane is bound, the peptide comprising the following peptide (1a) or (1b), or a fragment thereof: (1a) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9; and (1b) a peptide comprising the amino acid sequence of (1a) in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane.
摘要翻译：本发明的目的在于提供与聚二甲基硅氧烷（PDMS）具有亲和性的肽结合的聚二甲基硅氧烷基材，将目标蛋白质固定在聚二甲基硅氧烷基底上的方法; 对PDMS具有亲和性的肽; 编码对PDMS具有亲和力的肽的多核苷酸; 以及使用它的载体。（1a）或（1b）的肽，或其片段：（1a）包含由至少一个成员表示的氨基酸序列的肽，其中所述肽具有与聚二甲基硅氧烷的亲和性的肽结合到所述聚二甲基硅氧烷底物上，选自由SEQ ID NO：1至9组成的组; 和（1b）包含其中一个或多个氨基酸被缺失，取代和/或添加的（1a）的氨基酸序列的肽，所述肽对聚二甲基硅氧烷具有亲和性。

7. 发明公开

EP3816188A1 SEPARATING AGENT 有权
公开(公告)号：EP3816188A1
公开(公告)日：2021-05-05
申请号：EP19825188.6
申请日：2019-07-01
申请人： National University Corporation Kyoto Institute of Technology , Daicel Corporation
发明人： KUMADA, Yoichi , OKURA, Hiromichi , UCHIMURA, Seiichi
IPC分类号： C07K17/00 , C07K16/42 , G01N33/48 , C12N15/13
摘要： An object of the present invention is to provide a separating agent with an improved dynamic binding capacity (DBC) to a target substance and the object is achieved with a separating agent including a carrier and a protein, wherein the protein is a given protein, and a surface of the carrier and the added lysine residues in the protein are bound by chemical bonds.

8. 发明授权

EP2816110B1 PEPTIDE HAVING AFFINITY FOR SILICON NITRIDE (SI3N4), AND USE THEREFOR 有权
公开(公告)号：EP2816110B1
公开(公告)日：2018-11-28
申请号：EP13749185.8
申请日：2013-02-12
申请人： National University Corporation Kyoto Institute of Technology
发明人： KUMADA, Yoichi , KISHIMOTO, Michimasa , OTSUKA, Takeru
IPC分类号： C12N15/09 , C07K14/245 , C07K17/14 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N11/14 , C07K7/06 , C07K7/08 , C12N9/04 , C12N15/62
CPC分类号： C07K17/14 , C07K7/06 , C07K7/08 , C07K14/245 , C07K2319/00 , C07K2319/23 , C12N9/0006 , C12N11/14
摘要： The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride. The invention involves a peptide having an affinity for silicon nitride, the peptide comprising (1-1) a peptide having the amino acid sequence of one of SEQ ID NOS: 1, 2 and 23 to 27, (1-2) a peptide that has an affinity for silicon nitride and comprises an amino acid sequence obtained by deleting, adding, and/or substituting one or more amino acids in one of the amino acid sequences indicated in (1-1), or a fragment of one of the peptides.

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