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1. 发明公开

EP0121775A1 Novel expression plasmids and their use in the method for expressing prochymosin coding gene in E. coli 失效
标题翻译：在大肠杆菌中表达质粒和我的Verwendung im Verfahren zur表达von Prochymosin codierendem Gen。
公开(公告)号：EP0121775A1
公开(公告)日：1984-10-17
申请号：EP84102451.6
申请日：1984-03-07
申请人： Beppu, Teruhiko
发明人： Beppu, Teruhiko , Uozumi, Takeshi , Nishimori, Katsuhiko , Shimizu, Norio , Kawaguchi, Yoshiyuki , Hidaka, Makoto
IPC分类号： C12N15/00 , C12N9/50
CPC分类号： C12N9/6481 , C07K1/1136 , C07K2319/00 , C12N9/6483 , C12N15/71 , C12Y304/23004
摘要： improved methods are provided for replication and expression of prochymosin coding gene in E. coli. Novel expression plasmids having prochymosin coding gene operatively attached to the E. coli trp operon have been developed. These plasmids have been inserted into E. coli host cells by transformation, providing new strains to E. coli with high expression capability. The techniques for preparing such expression plasmids and E. coli strains derived therefrom are described. The modified cells, under appropriate cultivation conditions, produces a substantial amount of prochymosin which on activation (viz., in the form of chymosin) displays milk-clotting activity. The genetically engineered new strains of E. coli have been deposited with the Fermentation Research Institute, lbaragi, Japan under the Budapest Treaty. The methods are thus useful in the massive production of prochymosin by fermentation, and are superior to the known art in the field.
摘要翻译：提供改进的方法用于在大肠杆菌中复制和表达凝乳酶原编码基因。已经开发了具有可操作地连接到大肠杆菌trp操纵子的凝乳酶原编码基因的新型表达质粒。通过转化将这些质粒插入到大肠杆菌宿主细胞中，向具有高表达能力的大肠杆菌提供新的菌株。描述了制备这样的表达质粒和从其衍生的大肠杆菌菌株的技术。在适当的培养条件下，修饰的细胞产生大量的凝乳酶原，其在激活时（即以凝乳酶的形式）显示出乳凝血活性。根据布达佩斯条约，基因工程新菌株E. coil已经保藏在日本伊藤堡发酵研究所。因此，这些方法可用于通过发酵大规模生产凝乳酶原，并且优于本领域已知的技术。

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