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1. 发明公开

EP2436788A1 Methods for evaluating viral receptor/co-receptor usage and inhibitors of virus entry using recombinant virus assays 有权
标题翻译： / - - 共受体使用和抑制剂病毒进入测定用重组病毒一种用于评估的病毒受体的方法
公开(公告)号：EP2436788A1
公开(公告)日：2012-04-04
申请号：EP10011941.1
申请日：2002-06-04
申请人： Monogram BioSciences, Inc.
发明人： Whitcomb, Jeanette , Parkin, Neil T. , Huang, Wei , Petropoulos, Christos J.
IPC分类号： C12Q1/70
CPC分类号： G01N33/5044 , C12Q1/703 , G01N33/5008 , G01N33/502
摘要： The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable singal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

2. 发明公开

EP2444506A1 Methods for evaluating viral receptor/co-receptor usage and inhibitors of virus entry using recombinant virus assays 审中-公开
标题翻译： / - - 共受体使用和抑制剂病毒进入测定用重组病毒一种用于评估的病毒受体的方法
公开(公告)号：EP2444506A1
公开(公告)日：2012-04-25
申请号：EP10011940.3
申请日：2002-06-04
申请人： Monogram BioSciences, Inc.
发明人： Whitcomb, Jeanette , Parkin, Neil T. , Huang, Wei , Petropoulos, Christos J.
IPC分类号： C12Q1/70
CPC分类号： G01N33/5044 , C12Q1/703 , G01N33/5008 , G01N33/502
摘要： The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable singal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.
摘要翻译：本发明提供了用于鉴定化合物是否抑制病毒进入，其包含的细胞的方法：（a）获得的核酸编码来自被病毒感染的患者的病毒包膜蛋白; （B）共转染到第一单元（i）的核酸的步骤（a）的，以及（ii）其缺乏的核酸的病毒表达载体编码的包膜蛋白，以及指示器核酸，其包含产生一个检查检测的葛做的第一细胞产生的病毒颗粒，包括由所述核酸从患者获得的编码的包膜蛋白; （c）使在步骤（b）与化合物中的存在的第二细胞中产生的病毒颗粒，worin第二小区快车的细胞表面受体向其中病毒结合; （D）测定，以确定矿病毒颗粒的感染性通过所述第二小区产生的信号的量; 和（e）比较信号的步骤（d）测量与在不存在化合物的产生的信号的量的量，worin在化合物的存在下测量的信号的降低量表示DASS死化合物抑制病毒的进入到第二小区。

3. 发明公开

EP2808404A1 Evaluating viral receptor/coreceptor usage 有权
标题翻译：贝尔斯龙帝国毒死蜱 - / - CO-REZEPTORVERWENDUNG
公开(公告)号：EP2808404A1
公开(公告)日：2014-12-03
申请号：EP14166270.0
申请日：2003-02-14
申请人： Monogram Biosciences, Inc.
发明人： Petropoulos, Christos J. , Whitcomb, Jeanette M. , Huang, Wei , Wrin, Mary T.
IPC分类号： C12Q1/70
CPC分类号： G01N33/56988 , C07K14/005 , C07K14/16 , C07K14/162 , C07K16/08 , C07K16/10 , C12Q1/6897 , C12Q1/703 , G01N33/5044 , G01N33/5091 , G01N33/56983 , G01N2333/16
摘要： The present invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.
摘要翻译：本发明提供了鉴定化合物是否抑制病毒进入细胞的方法，该方法包括：（a）从病毒感染的患者获得编码病毒包膜蛋白的核酸; （b）共转染入第一细胞（i）步骤（a）的核酸，和（ii）缺乏编码包膜蛋白的核酸的病毒表达载体，其包含产生使得第一细胞产生包含由患者获得的核酸编码的包膜蛋白的病毒颗粒; （c）在化合物存在下将步骤（b）中产生的病毒颗粒与第二细胞接触，其中第二细胞表达病毒结合的细胞表面受体; （d）测量由第二细胞产生的信号量以确定病毒颗粒的感染性; 和（e）将步骤（d）中测量的信号量与在不存在化合物时产生的信号量进行比较，其中在化合物存在下测量的信号量减少表明化合物抑制病毒进入进入第二个细胞。

4. 发明授权

EP2363506B1 Evaluating viral receptor/coreceptor usage 有权
标题翻译：组合物和方法用于通过重组病毒测定的手段评估使用病毒受体/共受体，的以及病毒的进入抑制剂的
公开(公告)号：EP2363506B1
公开(公告)日：2014-04-30
申请号：EP11000425.6
申请日：2003-02-14
申请人： Monogram Biosciences, Inc.
发明人： Petropoulos, Christos J. , Whitcomb, Jeanette M. , Huang, Wei , Wrin, Mary T.
IPC分类号： C12Q1/70
CPC分类号： G01N33/56988 , C07K14/005 , C07K14/16 , C07K14/162 , C07K16/08 , C07K16/10 , C12Q1/6897 , C12Q1/703 , G01N33/5044 , G01N33/5091 , G01N33/56983 , G01N2333/16
摘要： The present invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

5. 发明授权

EP2436788B1 Methods for evaluating viral receptor/co-receptor usage and inhibitors of virus entry using recombinant virus assays 有权
标题翻译：用于评估病毒受体/共受体使用的方法和使用重组病毒分析的病毒进入抑制剂
公开(公告)号：EP2436788B1
公开(公告)日：2018-04-25
申请号：EP10011941.1
申请日：2002-06-04
申请人： Monogram BioSciences, Inc.
发明人： Whitcomb, Jeanette , Parkin, Neil T. , Huang, Wei , Petropoulos, Christos J.
IPC分类号： C12Q1/70
CPC分类号： G01N33/5044 , C12Q1/703 , G01N33/5008 , G01N33/502
摘要： The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable singal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

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