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1. 发明公开

EP2613145A1 TISSUE STAINING METHOD, TISSUE EVALUATION METHOD AND BIOSUBSTANCE DETECTION METHOD 有权
标题翻译： GEWEBEFÄRBEVERFAHREN，GEWEBEAUSWERTUNGSVERFAHREN UND VERFAHREN ZUM NACHWEIS VON BIOSUBSTANZEN
公开(公告)号：EP2613145A1
公开(公告)日：2013-07-10
申请号：EP11821360.2
申请日：2011-03-15
申请人： Konica Minolta Medical & Graphic, Inc. , Tohoku University
发明人： AIMIYA, Takuji , GOUDA, Hideki , OKADA, Hisatake , NAKANO, Yasushi , GONDA, Kohsuke , TAKEDA, Motohiro , OHUCHI, Noriaki
IPC分类号： G01N33/48 , G01N1/28 , G01N1/30 , G01N21/64 , G01N33/53 , G01N33/574
CPC分类号： G01N1/30 , B82Y15/00 , B82Y30/00 , G01N21/6428 , G01N33/582 , G01N33/588 , G01N33/74 , G01N33/743 , G01N2001/302 , G01N2021/6441 , G01N2333/71 , G01N2333/723 , G01N2333/91205
摘要： A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.
摘要翻译：一种组织染色方法，包括：用染色试剂染色组织，其中生物素识别位点与携带多种荧光物质的颗粒结合; 在染色的组织中，计数荧光点或测量荧光亮度; 并根据测定的荧光点数或荧光亮度，评价与上述组织中生物素识别位点相符的生物素的表达量。

2. 发明公开

EP2613138A1 BIOLOGICAL SUBSTANCE DETECTION METHOD 有权转让
标题翻译： VERFAHREN ZUM NACHWEIS生物学家STOFFE
公开(公告)号：EP2613138A1
公开(公告)日：2013-07-10
申请号：EP11821768.6
申请日：2011-08-30
申请人： Konica Minolta Medical & Graphic, Inc. , Tohoku University
发明人： TAKANASHI, Kensaku , GOUDA, Hideki , OKADA, Hisatake , NAKANO, Yasushi , GONDA, Kohsuke , TAKEDA, Motohiro , OHUCHI, Noriaki
IPC分类号： G01N21/64 , G01N21/27 , G01N33/48 , G01N33/53
CPC分类号： G01N21/6486 , G01N21/6428 , G01N21/6458 , G01N33/4833 , G01N33/582 , G01N2021/6439 , G01N2021/6441
摘要： A biological substance detection method for detecting a biological substance specifically in a pathological specimen, comprising a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.
摘要翻译：一种用于特异性检测病理标本中生物物质的生物物质检测方法，包括使用荧光标记物对病理标本进行免疫染色的步骤，用形态学观察目的的染色试剂（曙红）染色病理标本的步骤，以观察病理标本的形态，用激发光照射染色的病理标本以引起荧光发射并检测病理标本中的生物物质的步骤。在病理标本免疫染色的步骤中，使用在与曙红的激发波长区域不同的区域中出现激发波长的特殊荧光粒子作为荧光标记。

3. 发明公开

EP2833137A1 TISSUE STAINING METHOD 审中-公开
标题翻译：组织染色方法
公开(公告)号：EP2833137A1
公开(公告)日：2015-02-04
申请号：EP13768930.3
申请日：2013-03-26
申请人： Konica Minolta, Inc. , Tohoku University
发明人： GOUDA, Hideki , HOSHINO, Hideki , TAKANASHI, Kensaku , NAKANO, Yasushi , GONDA, Kohsuke , OHUCHI, Noriaki , WATANABE, Mika
IPC分类号： G01N33/48 , G01N1/30 , G01N21/27 , G01N21/64 , G01N33/53
CPC分类号： G01N1/30 , G01N21/6428 , G01N33/5082 , G01N33/52 , G01N33/535 , G01N33/552 , G01N33/56966 , G01N33/581 , G01N33/582
摘要： Provided is a method for staining a tissue enabling highly precise staining, by which the expression amount and/or the location of a biological substance in a tissue sample can be detected with a high quantitativity together with detailed information that can be obtained by bright field observation.
The tissue staining method of the present invention is a method for staining a tissue, in which both staining that allows bright field observation and fluorescence staining are carried out for the same specific biological substance.
摘要翻译：本发明提供一种能够实现高精度染色的组织的染色方法，通过该方法，可以以高定量性检测组织样品中的生物物质的表达量和/或位置，以及可以通过明视场观察获得的详细信息。本发明的组织染色方法是用于对组织进行染色的方法，其中针对相同的特定生物物质执行允许明视场观察和荧光染色的染色。

4. 发明公开

EP2755019A1 BIOLOGICAL SUBSTANCE DETECTION METHOD 有权转让
标题翻译： VERFAHREN ZUM NACHWEIS生物学家STOFFE
公开(公告)号：EP2755019A1
公开(公告)日：2014-07-16
申请号：EP12829813.0
申请日：2012-09-04
申请人： Konica Minolta, Inc. , Tohoku University
发明人： TAKANASHI, Kensaku , OKADA, Hisatake , NAKANO, Yasushi , GONDA, Kohsuke , OHUCHI, Noriaki , WATANABE, Mika
IPC分类号： G01N21/64
CPC分类号： G01N33/582 , G01N21/6428 , G01N33/56966 , G01N33/57492 , G01N2021/6439 , G01N2333/70596
摘要： The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of a part stained for morphological observation when the fluorescent label used for the immunostaining is observed.
摘要翻译：本发明提供了一种用于特异性检测来自病理标本的生物物质的生物物质检测方法，通过该方法，当使用荧光标记物进行免疫染色并同时进行用于形态学观察的染色剂的形态观察染色时，荧光检测结果即使通过用激发光照射使荧光标记和/或染色剂劣化，也可以适当地评价观察和免疫染色。根据本发明的生物物质检测方法的特征在于，当荧光标记物的形态学观察时，与染色的部分的亮度保持率相比，免疫染色部分的亮度保持率在80％至120％的范围内用于免疫染色观察。

5. 发明公开

EP2833144A1 METHOD FOR DETECTION BIOLOGICAL SUBSTANCE 有权
标题翻译： VERFAHREN ZUM NACHWEIS EINER生物学家SUBSTANZ
公开(公告)号：EP2833144A1
公开(公告)日：2015-02-04
申请号：EP13769111.9
申请日：2013-03-25
申请人： Konica Minolta, Inc. , Tohoku University
发明人： HOSHINO, Hidecki , GOUDA, Hideki , TAKANASHI, Kensaku , NAKANO, Yasushi , GONDA, Kohsuke , OHUCHI, Noriaki , WATANABE, Mika
IPC分类号： G01N33/543 , G01N33/53
CPC分类号： G01N33/54393 , G01N33/5306 , G01N33/54346 , G01N33/57415 , G01N33/57492 , G01N33/582 , G01N33/587
摘要： It is an object of the present invention to provide a detection method in which background noise is not increased even if a high-luminance fluorescent labeling material is used and which has an improved S/N ratio and high quantitativity and is advantageous for an immunohistological staining method. The present invention provides a detection method which is a method for detecting a specific biological substance using, as a color former, fluorescent substance-encapsulated nanoparticles to whose particle surfaces biological substance-recognizing molecules that specifically recognize a specific biological substance have been bonded, and which has an improved S/N ratio and high quantitativity and is advantageous for an immunohistological staining method because nanoparticles encapsulating no fluorescent substance are used as a blocking agent for preventing the fluorescent substance-encapsulated nanoparticles from being non-specifically adsorbed on a biological substance other than the specific biological substance.
摘要翻译：本发明的目的是提供一种即使使用高亮度荧光标记材料也不增加背景噪声并且具有改善的S / N比和高定量性并且有利于免疫组织染色的检测方法方法。本发明提供一种检测方法，其是使用作为成色剂的荧光物质包封的纳米粒子来检测特定生物物质的方法，其粒子表面特异性识别特定生物物质的生物物质识别分子已经结合，以及其具有改善的S / N比和高定量性，并且对于免疫组织染色方法是有利的，因为使用不含荧光物质的纳米颗粒用作阻止荧光物质包封的纳米颗粒非特异性吸附在生物物质上的阻断剂比具体的生物物质。

6. 发明公开

EP2833140A1 METHOD FOR DETECTING BIOLOGICAL MATERIAL 有权
标题翻译：检测生物材料的方法
公开(公告)号：EP2833140A1
公开(公告)日：2015-02-04
申请号：EP13770335.1
申请日：2013-03-28
申请人： Konica Minolta, Inc. , Tohoku University
发明人： TAKANASHI, Kensaku , GOUDA, Hideki , TAKANO, Keizu , HOSHINO, Hideki , NAKANO, Yasushi , GONDA, Kohsuke , OHUCHI, Noriaki , WATANABE, Mika
IPC分类号： G01N33/53 , G01N1/28 , G01N33/533
CPC分类号： G01N33/54393 , B82Y30/00 , G01N1/30 , G01N33/533 , G01N33/582 , G01N33/585
摘要： The present invention provides a staining method in which the fluorescent staining properties in a fluorescently-immunostained specimen are not reduced even when an oil-based mounting medium is used. The present invention also provides a method of preventing deterioration of a fluorescent label caused by irradiation with excitation light and improving the light resistance in a fluorescently-immunostained specimen obtained by the staining method. The biological substance detection method according to the present invention is a biological substance detection method for specifically detecting a biological substance from a pathological specimen, which comprises the steps of: immunostaining the specimen with a fluorescent label; immobilizing the thus stained specimen; and mounting the thus immobilized specimen using a mounting medium comprising an organic solvent not freely miscible with water. In the biological substance detection method, the above-described mounting medium further comprises a discoloration inhibitor.
摘要翻译：本发明提供一种染色方法，其中即使当使用油基安装介质时，荧光免疫染色试样中的荧光染色性能也不降低。本发明还提供了一种防止由激发光照射引起的荧光标记劣化并提高通过染色方法获得的荧光免疫染色标本的耐光性的方法。根据本发明的生物物质检测方法是用于从病理样本中特异性检测生物物质的生物物质检测方法，该方法包括以下步骤：用荧光标记对样本进行免疫染色; 固定如此染色的样本; 并使用包含不与水自由混溶的有机溶剂的安装介质来安装如此固定的样品。在生物物质检测方法中，上述安装介质还包含变色抑制剂。

7. 发明公开

EP2755026A1 METHOD FOR STAINING TISSUE 有权转让
标题翻译： VERFAHREN ZUMFÄRBENVON GEWEBEN
公开(公告)号：EP2755026A1
公开(公告)日：2014-07-16
申请号：EP12829474.1
申请日：2012-09-04
申请人： Konica Minolta, Inc. , Tohoku University
发明人： GOUDA, Hideki , OKADA, Hisatake , NAKANO, Yasushi , GONDA, Kohsuke , OHUCHI, Noriaki , WATANABE, Mika
IPC分类号： G01N33/48 , G01N1/30 , G01N21/78 , G01N33/53 , G01N33/536
CPC分类号： G01N33/569 , G01N1/30
摘要： It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected.
摘要翻译：本发明的目的是提供一种组织染色方法，其可以在单个部分和单个部分上观察关于组织形态的信息和关于待检测的抗原分子的生物物质的信息查看字段。本发明提供一种组织染色方法，其包括在单个组织切片上连续进行（A）HE（苏木精 - 伊红）染色和（B）组织化学染色，其中组织化学染色定义为组织化学技术通过使用待检测的生物物质和能够特异性结合待检测的生物物质的探针生物物质之间的结合反应，以可见的方式检测待检测的生物物质。

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