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1. 发明公开

EP3121291A1 PROBE REAGENT AND FISH USING PROBE REAGENT 审中-公开
标题翻译： SONDENREAGENS UND FISCH MIT VERWENDUNG DER SONDENREAGENS
公开(公告)号：EP3121291A1
公开(公告)日：2017-01-25
申请号：EP15765414.6
申请日：2015-03-20
申请人： Konica Minolta, Inc.
发明人： ISODA, Takeshi , SEKIGUCHI, Mitsuru , HATANAKA, Yutaka , KURIHARA, Yoshikazu
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6841 , C12Q2525/197 , C12Q2563/155
摘要： Provided are: a probe reagent which is capable of stably yielding a fluorescence signal in FISH while inhibiting non-specific adsorption by the use of a nucleic acid molecule having a smaller number of bases than a BAC probe; and FISH using the probe reagent. The probe reagent is for in situ hybridization and comprises: phosphor-integrated nanoparticles containing phosphors integrated therein; and a nucleic acid molecule having a prescribed nucleic acid sequence, which phosphor-integrated nanoparticles and nucleic acid molecule are bound with each other. This probe reagent enables to not only improve the accuracy of detecting a specific gene by inhibiting non-specific adsorption in FISH where a specific region of a chromosome is stained, but also obtain a fluorescence signal of a sufficient intensity for observation under a confocal microscope. When phosphor nanoparticles are used in the probe reagent, bright spots that are located at different depths of a tissue section can be detected simultaneously in a single field of view even when the tissue section is observed under a fluorescence microscope. Furthermore, the probe reagent enables to maintain a tissue section slide subjected to FISH in a state where bright spots are detectable over a longer period of time.
摘要翻译：提供：能够在FISH中稳定产生荧光信号的探针试剂，同时通过使用碱基少于BAC探针的核酸分子来抑制非特异性吸附; 和使用探针试剂的FISH。探针试剂用于原位杂交，并包含：含有荧光体的纳米磷光体; 和具有规定的核酸序列的核酸分子，磷光体整合的纳米粒子和核酸分子彼此结合。该探针试剂不仅可以通过抑制染色体的特定区域染色的FISH中的非特异性吸附来提高检测特异性基因的准确性，而且可以获得足够强度的荧光信号，以在共焦显微镜下观察。当在探针试剂中使用荧光体纳米颗粒时，即使在荧光显微镜下观察组织切片时，也可以在单一视野中同时检测位于组织切片不同深处的亮点。此外，探针试剂能够在更长的时间段内能够检测到亮点的状态下保持经受FISH的组织切片。

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