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1. 发明公开

EP2487249A1 METHOD AND APPARATUS FOR DISCRIMINATION OF UNDIFFERENTIATED PLYRIPOTENT STEM CELL, AND AUTOMATIC CULTURE METHOD AND APPARATUS 有权
标题翻译：方法和设备以示区别未分化的多能干细胞，并自动培养方法及其设备
公开(公告)号：EP2487249A1
公开(公告)日：2012-08-15
申请号：EP10821752.2
申请日：2010-10-07
申请人： Kawasaki Jukogyo Kabushiki Kaisha
发明人： WATAKABE, Keizo , NAKAMURA, Yohichi , OHJI, Osamu , NAKASHIMA, Katsumi
IPC分类号： C12Q1/04 , C12M1/34 , C12M3/00 , C12N5/07
CPC分类号： G01N33/5073 , G06K9/0014 , G06K9/00147
摘要： Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof.
A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony.
摘要翻译：提供能够分化多能干细胞的集落和未分化的多能干细胞的集落，并用于自动培养未分化的多能干细胞和系统及其方法之间进行区分的方法。分化的多能干细胞的组成未分化的多能干细胞，并且具有多层层叠的多能干细胞的多层细胞集落的未分化的组成集落分化的集落通过加工菌落包含在培养容器的多能干细胞的照片图像识别。具体地，具有亮度大于第一阈值A高的菌落是确定性的开采作为分化集落中，具有亮度等于或低于第一阈值A并且等于或高于第二阈值B中的菌落确定性开采作为未分化集落的和具有亮度大于第二阈值B以下的菌落确定性开采为多层集落。

2. 发明公开

EP2933324A1 CELL DETACHMENT DEVICE 有权
标题翻译： ZELLENABLÖSUNGSVORRICHTUNG
公开(公告)号：EP2933324A1
公开(公告)日：2015-10-21
申请号：EP13863213.8
申请日：2013-12-06
申请人： Kawasaki Jukogyo Kabushiki Kaisha
发明人： WATAKABE, Keizo , IIZAKA, Junji , HASUNAMA, Hitoshi , NAKASHIMA, Katsumi
IPC分类号： C12M1/00
CPC分类号： C12M33/08
摘要： A cell detachment device (1) includes: a base (10); a vessel holder (11), to which a culture vessel (2) is to be fitted; a guide mechanism (21) configured to guide reciprocating movement of the vessel holder (11); a collided member (33) configured to collide with the vessel holder (11) when the vessel holder (11) is moving; an urging member (31) configured to urge the culture vessel (2) toward the collided member (33); and a power imparting mechanism (41) configured to impart, to the vessel holder (11), power for causing the vessel holder (11) to move.
摘要翻译：细胞分离装置（1）包括：基底（10）; 要装配培养容器（2）的容器保持器（11）; 引导机构（21），其构造成引导所述容器保持器（11）的往复运动; 碰撞构件（33），其构造成当所述容器保持器（11）移动时与所述容器保持器（11）碰撞; 构造成将所述培养容器（2）推向所述碰撞构件（33）的施力构件（31）; 以及配置成向容器保持器（11）施加用于使容器保持器（11）移动的动力的动力施加机构（41）。

3. 发明授权

EP2487249B1 METHOD AND APPARATUS FOR DISCRIMINATION OF UNDIFFERENTIATED PLURIPOTENT STEM CELLS, AND AUTOMATIC CULTURE METHOD AND APPARATUS 有权
公开(公告)号：EP2487249B1
公开(公告)日：2018-06-13
申请号：EP10821752.2
申请日：2010-10-07
申请人： Kawasaki Jukogyo Kabushiki Kaisha
发明人： WATAKABE, Keizo , NAKAMURA, Yohichi , OHJI, Osamu , NAKASHIMA, Katsumi
IPC分类号： C12Q1/04 , C12M1/34 , C12M3/00 , C12N5/07 , G06K9/00
CPC分类号： G01N33/5073 , G06K9/0014 , G06K9/00147
摘要： Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof. A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony.

4. 发明授权

EP2933324B1 CELL DETACHMENT DEVICE 有权
标题翻译：细胞分离装置
公开(公告)号：EP2933324B1
公开(公告)日：2018-04-25
申请号：EP13863213.8
申请日：2013-12-06
申请人： Kawasaki Jukogyo Kabushiki Kaisha
发明人： WATAKABE, Keizo , IIZAKA, Junji , HASUNUMA, Hitoshi , NAKASHIMA, Katsumi
IPC分类号： C12M1/00
CPC分类号： C12M33/08
摘要： A cell detachment device (1) includes: a base (10); a vessel holder (11), to which a culture vessel (2) is to be fitted; a guide mechanism (21) configured to guide reciprocating movement of the vessel holder (11); a collided member (33) configured to collide with the vessel holder (11) when the vessel holder (11) is moving; an urging member (31) configured to urge the culture vessel (2) toward the collided member (33); and a power imparting mechanism (41) configured to impart, to the vessel holder (11), power for causing the vessel holder (11) to move.

5. 发明公开

EP2930233A1 DEVICE FOR AUTOMATICALLY PEELING CELLS AND SYSTEM FOR PEELING CELLS 审中-公开
标题翻译：用于自动地删除单元格和系统单元格中删除
公开(公告)号：EP2930233A1
公开(公告)日：2015-10-14
申请号：EP13860856.7
申请日：2013-12-06
申请人： Kawasaki Jukogyo Kabushiki Kaisha
发明人： WATAKABE, Keizo , SAKURAI, Takashi , ITO, Shimpei , NAKASHIMA, Katsumi
IPC分类号： C12M1/00 , C12M1/26 , C12M3/00
CPC分类号： C12M33/00 , C12M23/50 , C12M41/48 , C12M47/02
摘要： An automatic cell detachment device (5) includes: a placing board (51), to which a culture vessel (10) is to be fixed; a scraper (9) for scraping away cells adhering to an inner bottom surface of the culture vessel (10); and a chuck (8) including a pair of claws (81) configured to pinch an upper part of the scraper (9). The chuck (8) is lifted and lowered by a lifting/lowering mechanism, and is moved by a moving mechanism between the placing board (51) and a holding stand (53). The holding stand (53) holds replacement scrapers (9) in a state where an upper part of each replacement scraper (9) is exposed.
摘要翻译：一个自动细胞脱离装置（5）包括：载置板（51）到其上的培养容器（10）要被固定的; 用于刮去细胞秉承培养容器（10）的帧内底表面上的刮刀（9）; 和卡盘（8），包括被配置在所述刮板（9）的上部以捏的一对爪（81）。卡盘（8）被升高和降低通过提升/下降机构，并且通过载置板（51）和保持架（53）之间的移动机构移动。所述保持架（53）保持更换刮刀（9）在每次更换刮刀（9）的上部露出的状态。

6. 发明授权

EP2930233B1 DEVICE FOR AUTOMATICALLY PEELING CELLS AND SYSTEM FOR PEELING CELLS 有权
公开(公告)号：EP2930233B1
公开(公告)日：2020-02-05
申请号：EP13860856.7
申请日：2013-12-06
申请人： Kawasaki Jukogyo Kabushiki Kaisha
发明人： WATAKABE, Keizo , SAKURAI, Takashi , ITO, Shimpei , NAKASHIMA, Katsumi
IPC分类号： C12M1/00 , C12M1/26 , C12M3/00

7. 发明授权

EP2586872B1 METHOD AND DEVICE FOR IDENTIFYING MULTIPOTENT STEM CELL COLONY, AND METHOD AND DEVICE FOR AUTOMATIC CULTURING OF MULTIPOTENT STEM CELLS 有权
标题翻译：用于识别多个干细胞群的方法和装置以及用于多个干细胞的自动培养的方法和装置
公开(公告)号：EP2586872B1
公开(公告)日：2017-07-26
申请号：EP11797850.2
申请日：2011-06-22
申请人： Kawasaki Jukogyo Kabushiki Kaisha
发明人： WATAKABE, Keizo , SAKURAI, Takashi , OHJI, Osamu , NAKASHIMA, Katsumi
IPC分类号： C12Q1/02 , C12M1/34 , C12M3/00 , C12N5/071
CPC分类号： C12M41/46

8. 发明公开

EP2586872A1 METHOD AND DEVICE FOR IDENTIFYING MULTIPOTENT STEM CELL COLONY, AND METHOD AND DEVICE FOR AUTOMATIC CULTURING OF MULTIPOTENT STEM CELLS 有权
标题翻译：方法和设备的殖民地多能干细胞用于自动培育多能干细胞的识别与方法及装置
公开(公告)号：EP2586872A1
公开(公告)日：2013-05-01
申请号：EP11797850.2
申请日：2011-06-22
申请人： Kawasaki Jukogyo Kabushiki Kaisha
发明人： WATAKABE, Keizo , SAKURAI, Takashi , OHJI, Osamu , NAKASHIMA, Katsumi
IPC分类号： C12Q1/02 , C12M1/34 , C12M3/00 , C12N5/071
CPC分类号： C12M41/46
摘要： The present invention provides a method and device for achieving identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and to provide a method and device for achieving a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C1, and a second predetermined threshold is C2 (C1
摘要翻译：本发明提供了用于在无论是在培养容器中的细胞集落进行微分或多潜能干细胞的培养的场合未分化上实现识别的方法和装置，以及提供一种用于实现未分化的多能干细胞的快速培养的方法和设备，只含有未分化的多能干细胞的未分化集落是从其它类型的菌落通过菌落在截取的多能干细胞的培养容器的内部的图像处理的图像的圆形度C来识别。更具体地，当集落的圆的第一阈值是C1，和第二预定阈值是C2（C1

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