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1. 发明公开

EP4223879A1 ALPHA-AMYLASE VARIANT 审中-实审
公开(公告)号：EP4223879A1
公开(公告)日：2023-08-09
申请号：EP21875747.4
申请日：2021-09-29
申请人： Kao Corporation
发明人： HIOKI, Takahiro , SHAKU, Mao , KAWAHARA, Akihito
IPC分类号： C12N15/56 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/28
摘要： Provided is an α-amylase which can function at low temperatures while maintaining or enhancing stability and/or cleaning performance. An α-amylase mutant, which is a mutant of a parent α-amylase, the α-amylase mutant comprising one or more modifications to amino acid residues at positions corresponding to positions G5, S38, T49, Q96, N126, T129, G140, F153, Q167, G179, W186, E187, N192, M199, Y200, L203, Y205, D206, R211, K215, H240, S241, Y242, G244, E257, F259, K278, H283, S284, A288, H295, Y296, N303, T320, S331, L348, Y360, W408, L429, V430, G433, A434, W439, N471, G476, and G477 in the amino acid sequence of SEQ ID NO: 2, the parent α-amylase or α-amylase mutant having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 4.

2. 发明公开

EP4455289A1 AMYLASE-INCORPORATED CLEANING AGENT COMPOSITION 失效
公开(公告)号：EP4455289A1
公开(公告)日：2024-10-30
申请号：EP22911296.6
申请日：2022-12-21
申请人： Kao Corporation
发明人： SHAKU, Mao , HIOKI, Takahiro , KAWAHARA, Akihito
IPC分类号： C12N15/56 , C11D7/42 , C12N9/28 , C12N9/52
CPC分类号： C12N9/2414 , C11D3/386 , C12N9/52 , C12N9/24
摘要： Provided is an α-amylase-containing cleaning composition which has high amylolytic activity at low temperatures and also has high stability even in a protease-containing composition. A cleaning composition comprising an α-amylase mutant and protease, wherein the α-amylase mutant is a mutant of a parent α-amylase and comprises one or more amino acid residue substitutions at positions selected from the group consisting of positions corresponding to positions E187 and S241 in the amino acid sequence of SEQ ID NO: 2, and further one or more amino acid residue substitutions at positions selected from the group consisting of positions corresponding to positions N192, H240, and K278, wherein the parent α-amylase or α-amylase mutant has at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 4.

3. 发明公开

EP2451954A1 MODIFIED PROMOTER 有权转让
标题翻译： MODIFIZIERTE PROMOTER
公开(公告)号：EP2451954A1
公开(公告)日：2012-05-16
申请号：EP10773723.1
申请日：2010-10-18
申请人： Kao Corporation
发明人： KAWAHARA, Akihito , KODAMA, Hiroshi , ARA, Katsutoshi
IPC分类号： C12N15/113
CPC分类号： C12N15/75 , C12N1/20 , C12N15/11 , C12N15/66 , C12P21/02
摘要： The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.
摘要翻译：本发明提供了每个含有该启动子的修饰的启动子，表达载体和转化体，以及使用该转化体产生感兴趣的基因产物的方法。本发明提供一种修饰的启动子，其包含来源于属于芽孢杆菌属的细菌的启动子的核苷酸序列，其中至少一个选自以下的核苷酸序列已被修饰：由SEQ ID NO：1表示的核苷酸序列; 与SEQ ID NO：1所示核苷酸序列相当的核苷酸序列，除了其中一个或多个碱基被取代，缺失，添加或插入; 和相对于SEQ ID NO：1所示的核苷酸序列具有70％以上的序列同一性的核苷酸序列。

4. 发明公开

EP4438728A1 METHOD FOR CONSTRUCTING MUTANT LIBRARY POOL 审中-实审
公开(公告)号：EP4438728A1
公开(公告)日：2024-10-02
申请号：EP22895722.1
申请日：2022-11-21
申请人： Kao Corporation
发明人： HIOKI, Takahiro , TERAI, Mika , KAWAHARA, Akihito
IPC分类号： C12N15/10 , C12Q1/02 , C40B40/06
CPC分类号： C12N15/10 , C12Q1/02 , C40B40/06
摘要： To provide a method for preparing a highly diverse mutation library pool at a low price easily. A method for preparing a mutation library pool, comprising the following steps (a) to (c): (a) subjecting a template DNA and each of a plurality of mutagenic primer pairs to DNA synthesis reaction with a DNA polymerase to acquire DNA fragments having 5'-protruding ends, wherein each of the mutagenic primer pairs anneals to the template DNA, primers of each primer pair have regions complementary to each other, and at least one of the primers comprises a mutagenic site; (b) allowing a DNA ligase to act on the DNA fragments obtained in step (a); and (c) transforming host cells with the DNAs obtained in step (b).

5. 发明公开

EP4239071A1 MUTATED PROTEASE AND USE THEREOF 审中-实审
公开(公告)号：EP4239071A1
公开(公告)日：2023-09-06
申请号：EP21885842.1
申请日：2021-10-06
申请人： Kao Corporation
发明人： HIOKI, Takahiro , YAMASHITA, Daichi , KAWAHARA, Akihito
IPC分类号： C12N15/57 , C12N1/15 , C12N1/19 , C12N1/20 , C12N1/21 , C12N9/52 , C12N15/63
摘要： Provided are a polypeptide having a glycine-glycine bond-degrading activity and an improved microbicidal activity, and a method for using the same. A polypeptide consisting of the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having at least 80% identity thereto, the polypeptide having any modification selected from the group consisting of: a modification of an amino acid residue at position 116 in the amino acid sequence of SEQ ID NO: 2 or a position corresponding thereto; a modification of amino acid residues at position 170 in the amino acid sequence of SEQ ID NO: 2 or a position corresponding thereto, position 172 or a position corresponding thereto, position 173 or a position corresponding thereto, position 174 or a position corresponding thereto, position 178 or a position corresponding thereto, and position 179 or a position corresponding thereto; a modification of amino acid residues at position 170 in the amino acid sequence of SEQ ID NO: 2 or a position corresponding thereto, position 172 or a position corresponding thereto, position 173 or a position corresponding thereto, and position 174 or a position corresponding thereto; and a modification of amino acid residues at position 116 in the amino acid sequence of SEQ ID NO: 2 or a position corresponding thereto, position 170 or a position corresponding thereto, position 172 or a position corresponding thereto, position 173 or a position corresponding thereto, position 174 or a position corresponding thereto, position 178 or a position corresponding thereto, and position 179 or a position corresponding thereto, and the polypeptide having a glycine-glycine bond-degrading activity.

6. 发明公开

EP4238987A1 MODIFIED SIGNAL PEPTIDE 审中-实审
公开(公告)号：EP4238987A1
公开(公告)日：2023-09-06
申请号：EP21886174.8
申请日：2021-10-26
申请人： Kao Corporation
发明人： NISHIGUCHI, Hiroki , KAWAHARA, Akihito
IPC分类号： C07K16/00 , C12N1/21 , C12N15/63 , C12P21/02
摘要： A nucleic acid construct includes a polynucleotide encoding a protein of interest and a polynucleotide linked upstream of the polynucleotide and encoding a modified signal peptide. The modified signal peptide includes amino acid residues of X 1 X 2 X 3 X 4 X 5 X 6 at positions 1 to 6 as counted from the C-terminus, where X 1 is G, P, or A; X 2 is G, S, or A; and X 3 is P, S, or A, provided that X 1 X 2 X 3 is not GGP; X 4 is A; X 5 is S, H, or F; and X 6 is A and is located at the C-terminus of the modified signal peptide.

7. 发明公开

EP4183859A1 AMYLASE-INCORPORATED CLEANING AGENT COMPOSITION 审中-实审
公开(公告)号：EP4183859A1
公开(公告)日：2023-05-24
申请号：EP21843137.7
申请日：2021-07-07
申请人： Kao Corporation
发明人： HIOKI, Takahiro , KAWAHARA, Akihito
IPC分类号： C11D17/06 , C11D17/08 , C11D3/386 , C12N15/31 , C12N15/56 , C12N9/26 , C12N9/28
摘要： Provided is a cleaning composition containing α-amylase which exhibits high specific activity at low temperatures. The cleaning composition comprises one or more proteins selected from the group consisting of the following (A), (B), (C), and (D): (A) a protein consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 2, and having α-amylase activity; (B) a protein consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 4, and having α-amylase activity; (C) a protein consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 6, and having α-amylase activity; and (D) a protein consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 8, and having α-amylase activity.

8. 发明授权

EP2451954B1 MODIFIED PROMOTER 有权转让
公开(公告)号：EP2451954B1
公开(公告)日：2018-08-08
申请号：EP10773723.1
申请日：2010-10-18
申请人： Kao Corporation
发明人： KAWAHARA, Akihito , KODAMA, Hiroshi , ARA, Katsutoshi
IPC分类号： C12N15/113
CPC分类号： C12N15/75 , C12N1/20 , C12N15/11 , C12N15/66 , C12P21/02
摘要： The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.

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