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1. 发明公开

EP0718626A3 Calibrating and testing immunoassays to minimize interferences 失效
标题翻译： Kalibrierung undPrüfungvon Immunoassays zur Verminderung vonStörungen
公开(公告)号：EP0718626A3
公开(公告)日：1998-06-10
申请号：EP95309354
申请日：1995-12-21
申请人： JOHNSON & JOHNSON CLIN DIAG
发明人： JACOBS MERRIT N , KILDAL-BRANDT PAUL A , ARTER THOMAS C
IPC分类号： C12Q1/28 , G01N33/543 , G01N33/58 , G01N33/53
CPC分类号： G01N33/54386 , G01N33/581 , Y10S435/962 , Y10S435/97 , Y10S436/805 , Y10S436/815
摘要： A method for reducing interferent bias such as hemoglobin bias in immunoassays using dried slide test elements featuring peroxidase and leuco dye as the labeling mechanism. The method features the steps of:

a) calibrating an immunoassay of a target analyte by ascertaining the time at which the rate of change in density produced by a first set of the test elements for the target analyte on at least one liquid having a known amount of interferent of choice and a known amount of target analyte, crosses over or minimizes the difference compared to the rate of change in density produced by a second set of test elements substantially identical to the first set of test elements, on a liquid having the known amount of target analyte but a negligible amount of interferent of choice, and
b) conducting an assay of a patient sample of unknown amount of the target analyte by reading the rate of change in density in a third set of the test elements substantially identical to the first and second sets, caused by the sample, at a time corresponding to the cross-over time ascertained in step a).
摘要翻译：使用以过氧化物酶和无色染料为特征的干燥载玻片测试元件作为标记机制，减少免疫测定中的血红蛋白偏差等干扰性偏倚的方法。该方法具有以下步骤：a）通过确定由至少一种具有已知量的液体的目标分析物的第一组测试元件产生的密度变化率的时间来校准目标分析物的免疫测定的选择干扰物和已知量的目标分析物，与具有基本上与第一组测试元件基本相同的第二组测试元件产生的密度变化率相比，与已知的量的目标分析物交叉或最小化目标分析物的量，但是可选择的干扰物的量可忽略不计，以及b）通过读取第三组测试元件中的密度变化率来进行对未知量的目标分析物的患者样品的测定，其基本上与在对应于在步骤a）中确定的交叉时间的时间，由样本引起的第一和第二组。

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