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1. 发明公开

EP3170393A1 COLLAGEN POLYDISPERSIONS FOR THE TREATMENT OF CEREAL SEEDS AND PROCESS THEREOF 审中-公开
标题翻译：用于处理谷物种子的胶原多分散物及其加工
公开(公告)号：EP3170393A1
公开(公告)日：2017-05-24
申请号：EP15464007.2
申请日：2015-11-24
申请人： Institutul National de Cercetare-Dezvoltare Pentru Textile si Pielarie (INCDTP) Sucursala Institul de Cercetare Pielarie Incaltaminte (ICPI)
发明人： Niculescu, Mihaela-Doina , Gaidau, Carmen Cornelia , Epure, Doru-Gabriel , Gîdea, Mihai , Stepan, Emil
IPC分类号： A01N25/04 , A01N61/00 , A23J1/10 , C12P21/06
CPC分类号： A23J1/10 , A01N63/02 , C12P21/06 , A01N25/00 , A01N25/04
摘要： The invention relates to a product based on collagen polydispersions used for cereal seed treatment in order to stimulate growth, reduce the intake of pesticides and the process of manufacturing thereof.
Extraction of collagen polydispersions from leather waste occurs in two phases carried out through a compact process in the first phase through alkaline hydrolysis of chrome tanned leather waste, with 8-12% calcium oxide, or thermal hydrolysis of rawhide waste, at a solid/liquid ratio of 1/4, at a temperature of 70.....85°C, stirring for a period of 3.....5 hours and static until the next day without thermal input, followed by enzymatic hydrolysis with enzymatic preparation Alcalase 2.4L in concentration of 2.5.....4.5 enzyme units/total nitrogen gram, at a temperature of 63°C ± 2°C continuously stirring for 3.....5 hours for tanned leather waste, and static until the next day without thermal input in the case of extraction from rawhide waste. This process enables protein mass recovery in a proportion of 85.....95% and cleavage of peptide chains, yielding collagen polydispersions containing free amino acids, with average molecular weight of 4000-6000 Da for systemic cereal seed treatment, with effects on stimulating germination and increasing resistance to insect and fungal attacks.
摘要翻译：本发明涉及用于谷物种子处理的基于胶原多分散体的产品，以刺激生长，减少农药的摄入量及其制造过程。皮革废料中胶原多分散体的萃取分两个阶段进行，第一阶段通过一个紧凑的过程，通过碱性水解铬鞣制的皮革废料，8-12％的氧化钙或生皮废料的热水解，固/液比例为1/4，温度为70 ..... 85°C，搅拌3 ..... 5小时，静止至第二天无热输入，然后用酶制剂进行酶水解碱性蛋白酶2.4L浓度为2.5 ..... 4.5酶单位/总氮克，在63℃±2℃的温度下持续搅拌3 ........ 5小时，鞣制皮革废物，静止至第二天在没有热量输入的情况下从生皮废物中提取。该过程使得蛋白质质量回收率可达85％-95％，肽链断裂，产生含有游离氨基酸的胶原多分散体，平均分子量为4000-6000Da，用于全身谷物种子处理，对刺激发芽并增加对昆虫和真菌攻击的抵抗力。

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