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    • 1. 发明公开
    • CELL CULTURE DEVICE AND CELL CULTURE METHOD
    • ZELLKULTURVORRICHTUNG UND ZELLKULTURVERFAHREN
    • EP2725097A1
    • 2014-04-30
    • EP12803695.1
    • 2012-06-25
    • Hitachi, Ltd.
    • NAKAJIMA RyotaMATSUOKA ShizuKOBAYASHI Toyoshige
    • C12M3/00
    • C12M41/46C12M21/08C12M41/34C12M41/36
    • Provided is a cell culture device having a culture area for culturing the stem or progenitor cells, from which tissues are formed. The cell culture device includes an oxygen adjusting part that adjusts the oxygen supply in the culture area, a controller that controls the oxygen adjusting part, and another controller that controls a first oxygen supply during a first period of the period, in which the stem cells and the progenitor cells differentiate; and a second oxygen supply greater in amount than the first supply during a second period, during which the stem cells and the progenitor cells differentiate, out of the whole culture period, and that controls the oxygen adjusting part based on the growth progress of the stem cells and the progenitor cells through self-replication to change the first oxygen supply to the second oxygen supply.
    • 本发明提供一种细胞培养装置,其具有培养形成组织的干细胞或祖细胞的培养区域。 细胞培养装置包括调节培养区域中的氧供给量的氧调节部件,控制氧调节部件的控制器,以及控制在该期间的第一期间的第一氧供给量的控制器,其中,干细胞 祖细胞分化; 以及在整个培养期间干细胞和祖细胞分化的第二时期期间比第一次供应量大的第二氧供应,并且基于茎的生长进程来控制氧调节部分 细胞和祖细胞通过自我复制将第一氧供应改变为第二氧供应。
    • 5. 发明公开
    • CELL CULTURE DEVICE AND TRANSPORT DEVICE
    • 细胞培养装置和运输装置
    • EP2698427A1
    • 2014-02-19
    • EP12771514.2
    • 2012-04-04
    • Hitachi, Ltd.
    • KOBAYASHI ToyoshigeNAKAJIMA RyotaNOZAKI TakayukiMATSUOKA Shizu
    • C12M3/00C12M1/00
    • C12M29/00C12M23/14C12M23/50C12M37/00C12M41/14
    • The present invention provides a carrier device including a culture vessel and a flow channel, which have a closed structure, for supplying cell liquid or a culture medium into the flow channel; and a method of aseptically injecting liquid, such as the cell or the culture medium, into the culture vessel and the flow channel. The present invention provides a carrier jig 19 including a drive base 27, a culture vessel base 21 that holds a culture vessel 20, a culture medium base 32 that holds a cell bag 60 and the like for the culture medium and the cell, and a detachable flow channel 40, those of which are held by a lift 98 to be carried to a cell culture device. A part of the flow channel 40 and the cell bag 60 mounted on the carrier jig 19 are put into a clean space 78. The cell and the culture medium are supplied into the flow channel 40 by using the cell bag 60 and other bags in the clean space, and then, an injection port 89 is closed. The respective bases and the flow channel are simultaneously mounted to the cell culture device by using the carrier jig 19. Thus, the aseptic cell culture can be realized.
    • 本发明提供一种载体装置,其包括具有封闭结构的培养容器和流道,用于将细胞液或培养基供应到流道中; 以及将细胞或培养基等液体无菌注入培养容器和流路的方法。 本发明提供一种载体夹具19,该载体夹具19具有驱动基体27,保持培养容器20的培养容器基体21,保持培养基和细胞的细胞袋60等的培养基体32, 可拆卸的流动通道40,这些流动通道40由升降机98保持以携带至细胞培养装置。 安装在载体夹具19上的流道40和细胞袋60的一部分被放入清洁空间78中。细胞和培养基通过使用细胞袋60和其中的 清洁空间,然后关闭注入口89。 通过使用载体夹具19将各自的基部和流动通道同时安装到细胞培养装置。因此,可以实现无菌细胞培养。