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1. 发明公开

EP2298870A1 Method for producing scyllo-inositol 审中-公开
标题翻译：一种用于生产鲨肌醇的过程
公开(公告)号：EP2298870A1
公开(公告)日：2011-03-23
申请号：EP10182641.0
申请日：2004-10-14
申请人： Hokko Chemical Industry Co., Ltd.
发明人： Yamaguchi, Masanori , Kita, Yuichi , Mori, Tetsuya , Kanbe, Kenji , Tomoda, Akihiro , Takahashi, Atsushi , Ichikawa, Wakako
IPC分类号： C12N9/04 , C12P7/18 , C12P7/26 , C07C35/16 , C12P7/02
CPC分类号： C12P7/02 , C07C29/145 , C12N9/0004 , C12N9/0006 , C12P7/18 , C12P7/26
摘要： It is intended to provide a novel NAD + -independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD + ; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

2. 发明授权

EP2058390B1 Method for producing scyllo-inositol 有权
标题翻译：一种用于生产鲨肌醇的过程
公开(公告)号：EP2058390B1
公开(公告)日：2013-01-23
申请号：EP08019987.0
申请日：2004-10-14
申请人： HOKKO CHEMICAL INDUSTRY CO., LTD.
发明人： Yamaguchi, Masanori , Kita, Yuichi , Mori, Tetsuya , Kanbe, Kenji , Tomoda, Akihiro , Takahashi, Atsushi , Ichikawa, Wakako
IPC分类号： C12N9/04 , C12P7/18 , C12P7/26 , C07C35/16
CPC分类号： C12P7/02 , C07C29/145 , C12N9/0004 , C12N9/0006 , C12P7/18 , C12P7/26

3. 发明公开

EP2058390A1 Method for producing scyllo-inositol 有权
标题翻译： Verfahren zur Herstellung von Scyllo-Inositol
公开(公告)号：EP2058390A1
公开(公告)日：2009-05-13
申请号：EP08019987.0
申请日：2004-10-14
申请人： HOKKO CHEMICAL INDUSTRY CO., LTD.
发明人： Yamaguchi, Masanori , Kita, Yuichi , Mori, Tetsuya , Kanbe, Kenjii , Tomoda, Akihiro , Takhashi, Atsushi , Ichikawa, Wakako
IPC分类号： C12N9/04 , C12P7/18 , C12P7/26 , C07C35/16
CPC分类号： C12P7/02 , C07C29/145 , C12N9/0004 , C12N9/0006 , C12P7/18 , C12P7/26
摘要： It is intended to provide a novel NAD + -independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD + ; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.
摘要翻译：旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌蛋白; 在NADH或NADPH的存在下，立体选择性地将青蟹节小鼠降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 以及属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。通过使用这些酶或微生物，产生鲨肌醇。此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸络合物，将所述络合物与液体混合物分离，从而纯化鲨肌醇将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

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