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1. 发明公开

EP3902822A1 ANTI-PD-1 BINDING PROTEINS AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：EP3902822A1
公开(公告)日：2021-11-03
申请号：EP19906400.7
申请日：2019-12-27
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , ADLER, Adam, Shultz , MIZRAHI, Rena, Aviva , LIM, Yoong, Wearn , ASENSIO, Michael
IPC分类号： C07K14/725 , C07K14/74 , C07K16/00 , C12N15/10 , C12Q1/6806

2. 发明公开

EP4283295A3 RECOMBINANT POLYCLONAL PROTEINS AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：EP4283295A3
公开(公告)日：2024-03-06
申请号：EP23192977.9
申请日：2020-04-30
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , ADLER, Adam, Shultz , MIZRAHI, Rena, Aviva , LIM, Yoong, Weam , ASENSIO, Michael , KEATING, Sheila , LEONG, Renee , LEONG, Jackson
IPC分类号： C07K16/12 , C40B40/10 , G01N33/50 , G01N33/53 , A61P31/04 , A61P31/16 , A61P31/22 , A61P37/06
摘要： The present invention relates to a library of antibody proteins for use in treatment of a subject, wherein the antibody proteins specifically bind antigens, wherein the antibody proteins are encoded by a library of nucleic acids derived from plasmablasts and plasma cells and generated by (a) isolating the plasmablasts and plasma cells into single-cell reaction containers, thereby obtaining millions of single cells, (b) lysing the single cells, (c) purifying and capturing antibody-specific nucleic acids on solid supports such as beads from the millions of single cells in parallele,(d) amplifying nucleic acids that encode heavy and light chain immunoglobulins, (d) subsequently linking said nucleic acids into a library of fused constructs that encode both heavy and light chain immunoglobulin, thereby obtaining the library of nucleic acids wherein the library of nucleic acids comprises natively paired sequences of heavy and light chain immunoglobulins from the millions of single cells, as originally found in the plasmablasts and plasma cells.

3. 发明公开

EP3962941A2 RECOMBINANT POLYCLONAL PROTEINS AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：EP3962941A2
公开(公告)日：2022-03-09
申请号：EP20799376.7
申请日：2020-04-30
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , ADLER, Adam, Shultz , MIZRAHI, Rena, Aviva , LIM, Yoong, Weam , ASENSIO, Michael , KEATING, Sheila , LEONG, Renee , LEONG, Jackson
IPC分类号： C07K16/12 , C40B40/10 , G01N33/50 , G01N33/53

4. 发明授权

EP2652155B1 METHODS FOR MASSIVELY PARALLEL ANALYSIS OF NUCLEIC ACIDS IN SINGLE CELLS 有权
标题翻译：的程序对单个细胞NUCLEIC的大规模并行分析
公开(公告)号：EP2652155B1
公开(公告)日：2016-11-16
申请号：EP11848932.7
申请日：2011-12-16
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , MEYER, Everett, Hurteau
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C07K16/00 , C07K2317/622 , C12N15/1006 , C12N15/1065 , C12N15/1075 , C12Q1/6837 , C12Q1/6846 , C12Q1/6883 , C12Q1/6886 , C12Q1/6888 , C12Q2600/156 , C40B50/06 , C12Q2525/161 , C12Q2525/307 , C12Q2563/159
摘要： Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

5. 发明公开

EP3902821A1 ANTI-CTLA-4 BINDING PROTEINS AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：EP3902821A1
公开(公告)日：2021-11-03
申请号：EP19902920.8
申请日：2019-12-27
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , ADLER, Adam, Shultz , MIZRAHI, Rena, Aviva , LIM, Yoong, Wearn , ASENSIO, Michael , STONE, Erica, Lyn
IPC分类号： C07K14/725 , C07K14/74 , C07K16/00 , C12N15/00 , C12Q1/6806

6. 发明公开

EP3307905A1 RECOMBINANT FUSION PROTEINS AND LIBRARIES FROM IMMUNE CELL REPERTOIRES 审中-公开
标题翻译：重组融合蛋白和免疫细胞库的文库
公开(公告)号：EP3307905A1
公开(公告)日：2018-04-18
申请号：EP16808004.2
申请日：2016-05-18
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , ADLER, Adam , MIZRAHI, Rena
IPC分类号： C12Q1/66
CPC分类号： C07K16/005 , C07K2317/52 , C07K2317/55 , C07K2317/56 , C07K2317/622
摘要： Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

7. 发明公开

EP2652155A2 SYSTEM AND METHODS FOR MASSIVELY PARALLEL ANALYSIS OF NYCLEIC ACIDS IN SINGLE CELLS 有权
标题翻译：爱尔兰人VERFAHRENFÜRMASSIV PARALLELE ANALYZE VONNUKLEINSÄURENIN EINZELZELLEN
公开(公告)号：EP2652155A2
公开(公告)日：2013-10-23
申请号：EP11848932.7
申请日：2011-12-16
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , MEYER, Everett, Hurteau
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C07K16/00 , C07K2317/622 , C12N15/1006 , C12N15/1065 , C12N15/1075 , C12Q1/6837 , C12Q1/6846 , C12Q1/6883 , C12Q1/6886 , C12Q1/6888 , C12Q2600/156 , C40B50/06 , C12Q2525/161 , C12Q2525/307 , C12Q2563/159
摘要： Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.
摘要翻译：提供了方法和系统用于在乳液液滴或反应容器中对单细胞的大规模并行遗传分析。感兴趣的遗传基因座使用一组探针靶向单细胞，通过分子连锁和扩增技术形成融合复合物。提供了用于在至少10,000个细胞群体中的单细胞中的融合复合物的高通量大规模并行分析的方法。还提供了使用条形码序列将遗传信息追溯回小区的方法。

8. 发明公开

EP4175981A2 ANTI-CTLA-4 BINDING PROTEINS AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：EP4175981A2
公开(公告)日：2023-05-10
申请号：EP21833742.6
申请日：2021-07-02
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , ADLER, Adam, Shultz , MIZRAHI, Rena, Aviva , LIM, Yoong Wearn , ASENSIO, Michael , STONE, Erica, Lyn
IPC分类号： C07K14/725 , C07K14/74 , C07K16/00 , C07K16/28 , A61K39/395

9. 发明公开

EP3596216A1 SYSTEMS AND METHODS FOR MASSIVELY PARALLEL COMBINATORIAL ANALYSIS OF SINGLE CELLS 审中-公开
公开(公告)号：EP3596216A1
公开(公告)日：2020-01-22
申请号：EP18718956.8
申请日：2018-03-13
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , ADLER, Adam, Shultz , SPINDLER, Matthew, James , MIZRAHI, Rena, Aviva
IPC分类号： C12N15/10

10. 发明公开

EP4345107A2 RECOMBINANT FUSION PROTEINS AND LIBRARIES FROM IMMUNE CELL REPERTOIRES 审中-公开
公开(公告)号：EP4345107A2
公开(公告)日：2024-04-03
申请号：EP23219056.1
申请日：2016-05-18
申请人： Gigagen, Inc.
发明人： JOHNSON, David, Scott , ADLER, Adam , MIZRAHI, Rena
IPC分类号： C07K16/00
摘要： The invention relates to a recombinant immunoglobulin library generated by the method comprising: providing a plurality of recombinant fusion polynucleotides each comprising a first polynucleotide encoding a first variable domain, a second polynucleotide encoding a second variable domain, and a linker polynucleotide linking the first and second polynucleotides; expressing a plurality of recombinant fusion polypeptides encoded by said plurality of recombinant fusion polynucleotides to display said plurality of recombinant fusion polypeptides on a plurality of surfaces; enriching said plurality of recombinant fusion polynucleotides for binding to an antigen by exposing said plurality of surfaces to said antigen and selecting based on binding between said antigen and each of said plurality of recombinant fusion polypeptides; circularizing via Gibson Assembly one or more of said enriched recombinant fusion polynucleotides; inserting a third polynucleotide comprising a sequence encoding a promoter and a sequence encoding a constant region between said first and second polynucleotide in each of said circularized enriched recombinant fusion polynucleotides, thereby generating a plurality of recombinant immunoglobulin expression constructs, and inserting said plurality of recombinant immunoglobulin expression constructs into a plurality of host cells; and expressing said plurality of recombinant immunoglobulin expression construct in said plurality of host cells, thereby generating a recombinant immunoglobulin library, wherein each recombinant immunoglobulin comprises a linked heavy chain and light chain variable domain cognate pair from a single isolated cell.

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