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1. 发明公开

EP3225698A1 CLOSED NUCLEIC ACID STRUCTURES 有权
标题翻译：封闭的核酸结构
公开(公告)号：EP3225698A1
公开(公告)日：2017-10-04
申请号：EP17160148.7
申请日：2012-09-06
申请人： Gen-Probe Incorporated
发明人： NELSON, Norman C , CHELLISERRY, Jijumon , BRENTANO, Steven T , LYAKHOV, Dmitry , FRIEDENBERG, Matthew , SHAPIRO, Anne-Laure
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6855 , C12Q1/6869 , C12Q2521/501 , C12Q2525/155 , C12Q2525/161 , C12Q2525/191 , C12Q2525/301 , C12Q2525/307 , C12Q2533/101
摘要： There is described a method of forming a closed nucleic acid structure comprising a segment of a target nucleic acid, the method comprising: (a) contacting a target nucleic acid with a primer pair under amplification conditions, each of the primers of the pair including a 5' phosphate group and a 3' segment, the 3' segments of the primers being target-binding segments; thereby forming an amplified nucleic acid comprising duplex target nucleic acid flanked by the primers of the pair duplexed with their complementary segments, wherein each strand of the amplified nucleic acid includes a 5' phosphate group and a 3' hydroxyl group; (b) denaturing the amplified nucleic acid and contacting a strand of the denatured amplified nucleic acid with a stem-loop adaptor having a 5' phosphate group, a 5' segment, a 3' segment having a stem-loop structure, and a 3' hydroxyl group, wherein the 5' segment is complementary to a segment at the 5' end of the amplified nucleic acid strand; (c) annealing the 5' segment of the stem-loop adaptor to the 5' end segment of the amplified nucleic acid strand, thereby forming a partially duplex, two-stranded intermediate structure wherein the 5' phosphate group of the amplified nucleic acid strand is separated by a nick from the 3' hydroxyl group of the stem-loop adaptor; and (d) providing a ligase that seals the nick between the 5' phosphate group of the amplified nucleic acid strand and the 3' hydroxyl group of the stem-loop adaptor and additionally links the 5' phosphate group of the stem-loop adaptor to the 3' hydroxyl group of the amplified nucleic acid strand, thereby forming a closed nucleic acid structure.
摘要翻译：描述了形成包含靶核酸片段的闭合核酸结构的方法，所述方法包括：（a）在扩增条件下使靶核酸与引物对接触，所述一对引物中的每一个均包含 5'磷酸基团和3'区段，引物的3'区段是靶标结合区段; 从而形成包含双链靶核酸的扩增核酸，所述双链靶核酸侧接与其互补链段复合的一对引物，其中扩增核酸的每条链包含5'磷酸基团和3'羟基; （b）使扩增的核酸变性，并使变性扩增核酸的链与具有5'磷酸基团，5'区段，具有茎环结构的3'区段和3'区段的茎环接头接触 '羟基，其中5'区段与扩增的核酸链5'末端的区段互补; （c）将茎环接头的5'区段退火至扩增的核酸链的5'端区段，由此形成部分双链体双链中间结构，其中扩增的核酸链的5'磷酸基团通过茎环适配器的3'羟基的缺口分开; 和（d）提供连接酶，其将扩增的核酸链的5'磷酸基团与茎环适配体的3'羟基之间的切口密封并且另外将茎环适配体的5'磷酸基团连接到扩增的核酸链的3'羟基，由此形成闭合的核酸结构。

2. 发明公开

EP3121286A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权
标题翻译： VERFAHREN UND ZUSAMMENSETZUNGEN ZUR ERWEITERUNG VONNUKLEINSÄUREN
公开(公告)号：EP3121286A1
公开(公告)日：2017-01-25
申请号：EP16184379.2
申请日：2007-12-20
申请人： Gen-Probe Incorporated
发明人： ARNOLD, Lyle J Jr , BRENTANO, Steven T , CARLSON, James D , LYAKHOV, Dmitry , NELSON, Norman C , BECKER, Michael M
IPC分类号： C12P19/34
摘要： There is described a composition comprising: a TSU promoter oligonucleotide that includes a 5' promoter sequence, an internal first universal sequence (U1), and a 3' first target specific sequence (TS1) that binds specifically to a target sequence contained in a target nucleic acid, wherein the TSU promoter oligonucleotide is a TSU promoter primer that has a 3' terminus that is capable of being extended by a polymerase, or is a TSU promoter provider oligonucleotide that has a blocked 3' terminus that is incapable of being extended by a polymerase, a TSU non-promoter primer oligonucleotide made up of a 5' second universal sequence (U2) and a 3' second target specific sequence (TS2) which is different from the TS1, and a means for directly or indirectly joining the TSU promoter oligonucleotide to the TSU non-promoter primer oligonucleotide, thereby forming a target specific universal (TSU) primer complex.
摘要翻译：描述了一种组合物，其包含：TSU启动子寡核苷酸，其包含5'启动子序列，内部第一通用序列（U1）和特异性结合目标序列中的靶序列的3'第一靶特异性序列（TS1）核酸，其中所述TSU启动子寡核苷酸是具有能够被聚合酶延伸的3'末端的TSU启动子引物，或是具有阻止3'末端的TSU启动子提供者寡核苷酸，其不能被扩展由不同于TS1的5'第二通用序列（U2）和3'第二目标特异性序列（TS2）组成的聚合酶，TSU非启动子引物寡核苷酸，以及直接或间接连接TSU的装置启动子寡核苷酸引入TSU非启动子引物寡核苷酸，从而形成目标特异性通用（TSU）引物复合物。

3. 发明公开

EP4219741A3 CLOSED NUCLEIC ACID STRUCTURES 审中-实审
公开(公告)号：EP4219741A3
公开(公告)日：2023-08-23
申请号：EP22213215.1
申请日：2012-09-06
申请人： Gen-Probe Incorporated
发明人： NELSON, Norman C , CHELLISERRY, Jijumon
IPC分类号： C12Q1/68 , C12Q1/6806
摘要： There is provided a method of forming a closed nucleic acid structure comprising a target nucleic acid, the method comprising: (a) contacting a target nucleic acid with a first primer having a 3' target-binding segment, thereby forming a template for extension from the first primer; (b) forming a first extended nucleic acid strand duplexed to the target nucleic acid, the first extended strand comprising from 5'-3' the first primer and a segment complementary to the target nucleic acid; (c) denaturing the first extended strand from the target nucleic acid and contacting the first extended strand with a second primer having a 5' phosphate group and a 3' segment complementary to a segment of the first extended strand; (d) annealing the 3' segment of the second primer to the first extended strand, thereby forming a template for extension from the second primer; (e) forming a second extended nucleic acid strand duplexed to the first extended strand, the second extended strand comprising from 5'-3' the second primer, a segment complementary to the first extended strand and having a common sequence with the target nucleic acid, and a segment complementary to the first primer, the 5' end of the second extended strand being the phosphorylated 5' end of the second primer and the 3'end of the second extended strand having a 3' hydroxyl group; (f) denaturing the second extended strand from the first extended strand and contacting the second extended strand with an bridging oligonucleotide having a 5' segment and a 3' segment, the 5' segment being complementary to a segment at the 5' end of the second extended strand and the 3' segment being complementary to a segment at the 3' end of the second extended strand; (g) annealing the 5' segment of the bridging oligonucleotide to the 5' segment of the second extended strand and the 3' segment of the bridging oligonucleotide to the 3' segment of the second extended strand, thereby forming a partially duplex, two-stranded intermediate wherein the 5' phosphate group of the second extended strand is separated by a nick from the 3' hydroxyl group of the second extended strand; and (h) providing a ligase that seals the nick between the 5' phosphate and 3' hydroxyl groups of the second extended strand, thereby forming a closed nucleic acid structure.

4. 发明公开

EP4219740A3 CLOSED NUCLEIC ACID STRUCTURES 审中-实审
公开(公告)号：EP4219740A3
公开(公告)日：2023-08-16
申请号：EP22213207.8
申请日：2012-09-06
申请人： Gen-Probe Incorporated
发明人： NELSON, Norman C , CHELLISERRY, Jijumon
IPC分类号： C12Q1/68
摘要： There is provided a method of forming a closed nucleic acid structure from a target nucleic acid, comprising: (a) contacting a target nucleic acid with a primer pair under PCR conditions, each of the primers having a 5' phosphate group, thereby forming an amplified nucleic acid comprising a segment of the target nucleic acid flanked by the primers duplexed with their complementary segments; (b) contacting the amplified nucleic acid with a terminal deoxynucleotidyl transferase (TdT) and a first deoxynucleotide thereby extending the 3' ends of the amplified nucleic acid with a homo-oligomeric tail comprising the first deoxynucleotide; (c) annealing an adaptor having a 5' region and a 3' region to the extended nucleic acid, the 5' region of the adaptor having a 5' phosphate group and comprising a stem-loop structure, and the 3' region of the adaptor being complementary to the homo-oligomeric tail of the extended nucleic acid, wherein the 3' region of the adaptor hybridizes to the homo-oligomeric tail thereby forming an adaptor-capped nucleic acid; (d) contacting the adaptor-capped nucleic acid with the first deoxynucleotide and/or a second deoxynucleotide which is complementary to the first deoxynucleotide, and a DNA polymerase, thereby filling in any gap in the adaptor-capped nucleic acid with the first or the second deoxynucleotide; and (e) contacting the closed nucleic acid structure with a ligase which seals nicks in the closed nucleic acid structure.

5. 发明公开

EP3640348A1 MULTIPHASE NUCLEIC ACID AMPLIFICATION 审中-公开
公开(公告)号：EP3640348A1
公开(公告)日：2020-04-22
申请号：EP19207485.4
申请日：2013-08-30
申请人： Gen-Probe Incorporated
发明人： NELSON, Norman C , ARNOLD, Lyle J Jr , DAI, Lizhong , PHELPS, Steven S. , CHELLISERRY, Jujumon
IPC分类号： C12Q1/6865
摘要： There is disclosed a method of amplifying a target nucleic acid sequence present in a sample, the method comprising the steps of: (a) performing a first phase linear amplification reaction that amplifies the target nucleic acid sequence under conditions that do not support exponential amplification thereof; wherein the first phase linear amplification reaction generates a first amplification product; and (b) performing a second phase amplification reaction under conditions allowing exponential amplification of the first amplification product, thereby generating a second amplification product that comprises the target nucleic acid sequence or the complement thereof.

6. 发明公开

EP3095873A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权
标题翻译：方法和组合物放大核酸的
公开(公告)号：EP3095873A1
公开(公告)日：2016-11-23
申请号：EP16175562.4
申请日：2007-12-20
申请人： Gen-Probe Incorporated
发明人： ARNOLD, Lyle J Jr , BRENTANO, Steven T , CARLSON, James D , LYAKHOV, Dmitry , NELSON, Norman C , BECKER, Michael M
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q2525/155 , C12Q2525/186 , C12Q2525/197 , C12Q2531/143 , C12Q2565/543
摘要： There is described a target capture reaction mixture for separating a target nucleic acid from a sample, the reaction mixture comprising:
a. a target specific universal (TSU) primer complex made up of
i. a TSU promoter oligonucleotide comprising a 5' promoter sequence, an internal first universal sequence (U1), and a 3' first target specific sequence (TS1) that binds specifically to a target sequence contained in a target nucleic acid, wherein the TSU promoter oligonucleotide is a TSU promoter primer that has a 3' terminus that is capable of being extended by a polymerase, or is a TSU promoter provider oligonucleotide that has a blocked 3' terminus that is incapable of being extended by a polymerase, directly or indirectly joined to,
ii. a TSU non-promoter primer oligonucleotide made up of a 5' second universal sequence (U2) and a 3' second target specific sequence (TS2) which is different from the TS1,
wherein the TSU promoter oligonucleotide is joined to the TSU non-promoter primer via:
(A) a covalent linkage that is a polynucleotide linker sequence or a non-nucleotide abasic linker compound;
(B) a hybridization complex between a first sequence on the TSU promoter oligonucleotide and a second sequence on the TSU non-promoter primer that is complementary to the first sequence on the TSU promoter oligonucleotide; or
(C) a hybridization complex that includes an S-oligonucleotide that contains a first sequence complementary to a sequence in the TSU promoter oligonucleotide and a second sequence complementary to a sequence in the TSU non-promoter primer oligonucleotide; and
b. a target specific capture oligonucleotide that contains a target specific sequence (TS3) that hybridizes specifically to a sequence in the target nucleic acid that is different from the sequence in the target nucleic acid that hybridizes to the TS sequence of the TSU promoter oligonucleotide or the TS sequence of the TSU non-promoter primer, and contains a means for binding the target nucleic acid to a solid support.

7. 发明公开

EP4219741A2 CLOSED NUCLEIC ACID STRUCTURES 审中-实审
公开(公告)号：EP4219741A2
公开(公告)日：2023-08-02
申请号：EP22213215.1
申请日：2012-09-06
申请人： Gen-Probe Incorporated
发明人： NELSON, Norman C , CHELLISERRY, Jijumon
IPC分类号： C12Q1/68
摘要： There is provided a method of forming a closed nucleic acid structure comprising a target nucleic acid, the method comprising: (a) contacting a target nucleic acid with a first primer having a 3' target-binding segment, thereby forming a template for extension from the first primer; (b) forming a first extended nucleic acid strand duplexed to the target nucleic acid, the first extended strand comprising from 5'-3' the first primer and a segment complementary to the target nucleic acid; (c) denaturing the first extended strand from the target nucleic acid and contacting the first extended strand with a second primer having a 5' phosphate group and a 3' segment complementary to a segment of the first extended strand; (d) annealing the 3' segment of the second primer to the first extended strand, thereby forming a template for extension from the second primer; (e) forming a second extended nucleic acid strand duplexed to the first extended strand, the second extended strand comprising from 5'-3' the second primer, a segment complementary to the first extended strand and having a common sequence with the target nucleic acid, and a segment complementary to the first primer, the 5' end of the second extended strand being the phosphorylated 5' end of the second primer and the 3'end of the second extended strand having a 3' hydroxyl group; (f) denaturing the second extended strand from the first extended strand and contacting the second extended strand with an bridging oligonucleotide having a 5' segment and a 3' segment, the 5' segment being complementary to a segment at the 5' end of the second extended strand and the 3' segment being complementary to a segment at the 3' end of the second extended strand; (g) annealing the 5' segment of the bridging oligonucleotide to the 5' segment of the second extended strand and the 3' segment of the bridging oligonucleotide to the 3' segment of the second extended strand, thereby forming a partially duplex, two-stranded intermediate wherein the 5' phosphate group of the second extended strand is separated by a nick from the 3' hydroxyl group of the second extended strand; and (h) providing a ligase that seals the nick between the 5' phosphate and 3' hydroxyl groups of the second extended strand, thereby forming a closed nucleic acid structure.

8. 发明公开

EP4219740A2 CLOSED NUCLEIC ACID STRUCTURES 审中-实审
公开(公告)号：EP4219740A2
公开(公告)日：2023-08-02
申请号：EP22213207.8
申请日：2012-09-06
申请人： Gen-Probe Incorporated
发明人： NELSON, Norman C , CHELLISERRY, Jijumon
IPC分类号： C12Q1/68
摘要： There is provided a method of forming a closed nucleic acid structure from a target nucleic acid, comprising: (a) contacting a target nucleic acid with a primer pair under PCR conditions, each of the primers having a 5' phosphate group, thereby forming an amplified nucleic acid comprising a segment of the target nucleic acid flanked by the primers duplexed with their complementary segments; (b) contacting the amplified nucleic acid with a terminal deoxynucleotidyl transferase (TdT) and a first deoxynucleotide thereby extending the 3' ends of the amplified nucleic acid with a homo-oligomeric tail comprising the first deoxynucleotide; (c) annealing an adaptor having a 5' region and a 3' region to the extended nucleic acid, the 5' region of the adaptor having a 5' phosphate group and comprising a stem-loop structure, and the 3' region of the adaptor being complementary to the homo-oligomeric tail of the extended nucleic acid, wherein the 3' region of the adaptor hybridizes to the homo-oligomeric tail thereby forming an adaptor-capped nucleic acid; (d) contacting the adaptor-capped nucleic acid with the first deoxynucleotide and/or a second deoxynucleotide which is complementary to the first deoxynucleotide, and a DNA polymerase, thereby filling in any gap in the adaptor-capped nucleic acid with the first or the second deoxynucleotide; and (e) contacting the closed nucleic acid structure with a ligase which seals nicks in the closed nucleic acid structure.

9. 发明授权

EP3121286B1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权
公开(公告)号：EP3121286B1
公开(公告)日：2019-11-20
申请号：EP16184379.2
申请日：2007-12-20
申请人： Gen-Probe Incorporated
发明人： ARNOLD, Lyle J Jr , BRENTANO, Steven T , CARLSON, James D , LYAKHOV, Dmitry , NELSON, Norman C , BECKER, Michael M
IPC分类号： C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6881

10. 发明授权

EP3225698B1 CLOSED NUCLEIC ACID STRUCTURES 有权
公开(公告)号：EP3225698B1
公开(公告)日：2019-07-31
申请号：EP17160148.7
申请日：2012-09-06
申请人： Gen-Probe Incorporated
发明人： NELSON, Norman C , CHELLISERRY, Jijumon , BRENTANO, Steven T , LYAKHOV, Dmitry , FRIEDENBERG, Matthew , SHAPIRO, Anne-Laure
IPC分类号： C12Q1/6855 , C12Q1/6869

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