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1. 发明公开

EP2414547A1 MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 有权
标题翻译：靶核酸的多重底漆GAIN方法条形码
公开(公告)号：EP2414547A1
公开(公告)日：2012-02-08
申请号：EP10759511.8
申请日：2010-04-02
申请人： Fluidigm Corporation
发明人： MAY, Andrew , CHEN, Peilin , WANG, Jun , KAPER, Fiona , ANDERSON, Megan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
摘要： In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

2. 发明授权

EP2414547B1 MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 有权
标题翻译：麦克风ER EN EN EN R R EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN
公开(公告)号：EP2414547B1
公开(公告)日：2014-03-12
申请号：EP10759511.8
申请日：2010-04-02
申请人： Fluidigm Corporation
发明人： MAY, Andrew , CHEN, Peilin , WANG, Jun , KAPER, Fiona , ANDERSON, Megan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629

3. 发明公开

EP2569447A2 ASSAYS FOR THE DETECTION OF GENOTYPE, MUTATIONS, AND/OR ANEUPLOIDY 审中-公开
标题翻译：测定法检测基因型，突变或异倍体
公开(公告)号：EP2569447A2
公开(公告)日：2013-03-20
申请号：EP11780930.1
申请日：2011-05-16
申请人： Fluidigm Corporation , Pieprzyk, Martin
发明人： PIEPRZYK, Martin , JONES, Robert C. , LIVAK, Kenneth J. , MAY, Andrew , MIR, Alain , QIN, Jian , RAMAKRISHNAN, Ramesh , SPURGEON, Sandra , WANG, Jun , ZIMMERMANN, Bernhard G.
IPC分类号： C12Q1/68 , G01N33/52 , G06F19/18
CPC分类号： C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
摘要： The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

4. 发明授权

EP2707507B1 PROBE BASED NUCLEIC ACID DETECTION 有权
标题翻译：基于探针的核酸检测
公开(公告)号：EP2707507B1
公开(公告)日：2017-11-01
申请号：EP12782232.8
申请日：2012-05-09
申请人： Fluidigm Corporation
发明人： LIVAK, Kenneth J. , MYERS, Stacey , WANG, Xiaohui , WANG, Jun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2525/151

5. 发明公开

EP2707507A1 PROBE BASED NUCLEIC ACID DETECTION 有权
标题翻译： PROBENBASIERTERNUKLEINSÄURENACHWEIS
公开(公告)号：EP2707507A1
公开(公告)日：2014-03-19
申请号：EP12782232.8
申请日：2012-05-09
申请人： Fluidigm Corporation
发明人： LIVAK, Kenneth J. , MYERS, Stacey , WANG, Xiaohui , WANG, Jun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2525/151
摘要： The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
摘要翻译：本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了包含调节序列和核苷酸标签识别序列的具有解链温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，扩增PCR扩增中的标记的靶核酸序列使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

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