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    • 9. 发明授权
    • CULTURES WITH IMPROVED PHAGE RESISTANCE
    • KULTUREN MIT VERBESSERTER PHAGENRESISTENZ
    • EP2126130B1
    • 2015-03-25
    • EP08726285.3
    • 2008-02-29
    • DuPont Nutrition Biosciences ApS
    • BARRANGOU, RodolpheFREMAUX, ChristopheHORVATH, PhilippeROMERO, DennisBOYAVAL, Patrick
    • C12Q1/68
    • C12N15/746A23C9/123A23C9/1238A23C2220/202A23Y2240/75C12N1/20C12N2795/00011C12Q1/70C12Q2525/151
    • The present invention provides methods and compositions related to modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some preferred embodiments, the present invention provides compositions and methods for the use of one or more cas genes or proteins for modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some embodiments, the present invention provides methods and compositions that find use in the development and use of strain combinations and starter culture rotations. In additional embodiments, the present invention provides methods for labelling and/or identifying bacteria. In some preferred embodiments, the present invention provides methods for the use of CRISPR loci to determine the potential virulence of a phage against a cell and the use of CRISPR-cas to modulate the genetic sequence of a phage for increased virulence level. In still further embodiments, the present invention provides means and compositions for the development and use of phages as biocontrol agents.
    • 本发明提供与调节细胞对靶核酸或其转录产物的抗性有关的方法和组合物。 在一些优选的实施方案中,本发明提供了使用一种或多种cas基因或蛋白质调节细胞对靶核酸或其转录产物的抗性的组合物和方法。 在一些实施方案中,本发明提供了用于开发和使用菌株组合和起始培养旋转的方法和组合物。 在另外的实施方案中,本发明提供用于标记和/或鉴定细菌的方法。 在一些优选的实施方案中,本发明提供了使用CRISPR基因座确定噬菌体对细胞的潜在毒力的方法,以及使用CRISPR-cas调节噬菌体的遗传序列以增加毒力水平。 在另外的实施方案中,本发明提供用于开发和使用噬菌体作为生物防治剂的方法和组合物。