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1. 发明公开

EP3209774A1 USE OF PROLINE TOLERANT TRIPEPTIDYL PEPTIDASES IN FEED ADDITIVE COMPOSITIONS 审中-公开
标题翻译：脯氨酸耐受性三磷酸肽在饲料添加剂组合物中的应用
公开(公告)号：EP3209774A1
公开(公告)日：2017-08-30
申请号：EP15794483.6
申请日：2015-10-23
申请人： DuPont Nutrition Biosciences ApS
发明人： HAANING, Svend , KRAGH, Karsten Matthias , SHIPOVSKOV, Stepan , MIASNIKOV, Andrei , MA, Maria , MILLAN, Luis Fernando Romero , BARNARD, Luke , YU, Shukun , MEINJOHANNS, Ernst , BAK, Steffen Yde , NURMINEN, Paivi , PUTAALA, Heli
IPC分类号： C12N9/48 , A23J3/34 , C12P21/06
摘要： A method of preparing a feed additive composition comprising: (a) admixing at least one proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having (i) (A) Proline at P1; and (B) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids at P1; or (ii) (a′) Proline at P1′; and (b′) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids or amines at P1′; and one or more ingredients selected from the group consisting of a salt, polyol including sorbitol and glycerol, wheat or a wheat component, sodium acetate, sodium acetate trihydrate, potassium sorbate Talc, polyvinyl alcohol (PVA), benzoate, sorbiate, 1,3-propane diol, glucose, parabens, sodium chloride, citrate, metabisulfite, formate or a combination thereof; and (b) optionally packaging as well as uses of such proline tolerant tripeptidyl peptidases, feed additive compositions, feed additive kits, feeds or feedstuffs and/or premixes.
摘要翻译：一种制备饲料添加剂组合物的方法，包括：（a）混合至少一种主要具有外肽酶活性的脯氨酸耐受性三肽肽酶，其中所述脯氨酸耐受性三肽肽酶能够从具有（i）（ A）P1处的脯氨酸; （B）选自丙氨酸，精氨酸，天冬酰胺，天冬氨酸，半胱氨酸，谷氨酰胺，谷氨酸，甘氨酸，组氨酸，异亮氨酸，亮氨酸，赖氨酸，甲硫氨酸，苯丙氨酸，丝氨酸，苏氨酸，色氨酸，酪氨酸，缬氨酸或合成的氨基酸 P1处的氨基酸; 或（ii）（a'）PV处的脯氨酸; 和（b'）选自丙氨酸，精氨酸，天冬酰胺，天冬氨酸，半胱氨酸，谷氨酰胺，谷氨酸，甘氨酸，组氨酸，异亮氨酸，亮氨酸，赖氨酸，甲硫氨酸，苯丙氨酸，丝氨酸，苏氨酸，色氨酸，酪氨酸，缬氨酸或 PV处的合成氨基酸或胺; 以及一种或多种选自盐，包括山梨醇和甘油的多元醇，小麦或小麦组分，乙酸钠，乙酸钠三水合物，山梨酸钾滑石，聚乙烯醇（PVA），苯甲酸酯，山梨酸酯，1,3 丙二醇，葡萄糖，对羟基苯甲酸酯，氯化钠，柠檬酸盐，偏亚硫酸氢盐，甲酸盐或其组合; 和（b）任选地包装以及这种耐受脯氨酸的三肽肽酶，饲料添加剂组合物，饲料添加剂药盒，饲料或饲料和/或预混合物的用途。

3. 发明授权

EP3209773B1 PROLINE TOLERANT TRIPEPTIDYL PEPTIDASES AND USES THEREOF 有权
公开(公告)号：EP3209773B1
公开(公告)日：2020-03-18
申请号：EP15793724.4
申请日：2015-10-23
申请人： DuPont Nutrition Biosciences ApS
发明人： KRAGH, Karsten Matthias , MEINJOHANNS, Ernst , MIASNIKOV, Andrei , MA, Maria , EISELE, Thomas , HAANING, Svend , DEGN, Peter Edvard , BAK, Steffen Yde
IPC分类号： C12N9/48 , C12P21/06 , A23J3/34 , A23K10/14

4. 发明公开

EP3931314A1 PROTEIN HYDROLYSATES WITH INCREASED YIELD OF N-TERMINAL AMINO ACID 审中-实审转让
公开(公告)号：EP3931314A1
公开(公告)日：2022-01-05
申请号：EP20714377.7
申请日：2020-02-25
申请人： DuPont Nutrition Biosciences ApS
发明人： DEGN, Peter Edvard , GU, Xiaogang , KRAGH, Karsten Matthias , SORG, Robin Anton , BAK, Steffen Yde , HAANING, Svend , TANG, Xinyue , HAO, Helong , KOLKMAN, Marc Anton Bernhard
IPC分类号： C12N9/48

5. 发明公开

EP3420097A1 METHOD FOR PRODUCING A PROTEIN HYDROLYSATE EMPLOYING AN ASPERGILLUS FUMIGATUS TRIPEPTIDYL PEPTIDASE 审中-公开
公开(公告)号：EP3420097A1
公开(公告)日：2019-01-02
申请号：EP17709843.1
申请日：2017-02-21
申请人： DuPont Nutrition Biosciences ApS
发明人： KRAGH, Karsten Matthias , HAANING, Svend , MEINJOHANNS, Ernst , DEGN, Peter Edvard , BAK, Steffen Yde , EISELE, Thomas
IPC分类号： C12P21/06 , A23J3/34 , C12N9/48 , A23K10/10

6. 发明公开

EP3314004A1 AMINOPEPTIDASES FOR PROTEIN HYDROLYZATES 审中-实审转让
标题翻译：氨基肽酶用于蛋白质水解产物
公开(公告)号：EP3314004A1
公开(公告)日：2018-05-02
申请号：EP16739307.3
申请日：2016-06-27
申请人： DuPont Nutrition Biosciences ApS
发明人： KRAGH, Karsten Matthias , HAANING, Svend , MEINJOHANNS, Ernst , EISELE, Thomas , DEGN, Peter Edvard , BAK, Steffen Yde
IPC分类号： C12P21/06 , C12N9/48 , C12N15/80
CPC分类号： C12N9/485 , C07K2319/02 , C12C1/047 , C12C5/004 , C12C11/003 , C12N1/14 , C12N15/80 , C12P21/06 , C12Y304/11
摘要： The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.

7. 发明公开

EP3209773A1 PROLINE TOLERANT TRIPEPTIDYL PEPTIDASES AND USES THEREOF 有权
标题翻译：脯氨酸耐受性三磷酸肽及其用途
公开(公告)号：EP3209773A1
公开(公告)日：2017-08-30
申请号：EP15793724.4
申请日：2015-10-23
申请人： DuPont Nutrition Biosciences ApS
发明人： KRAGH, Karsten Matthias , MEINJOHANNS, Ernst , MIASNIKOV, Andrei , MA, Maria , EISELE, Thomas , HAANING, Svend , DEGN, Peter Edvard , BAK, Steffen Yde
IPC分类号： C12N9/48 , C12P21/06 , A23J3/34
摘要： A method of preparing a feed additive composition comprising: (a) admixing at least one proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having (i) (A) Proline at P1; and (B) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids at P1; or (ii) (a′) Proline at P1′; and (b′) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids or amines at P1′; and one or more ingredients selected from the group consisting of a salt, polyol including sorbitol and glycerol, wheat or a wheat component, sodium acetate, sodium acetate trihydrate, potassium sorbate Talc, polyvinyl alcohol (PVA), benzoate, sorbiate, 1,3-propane diol, glucose, parabens, sodium chloride, citrate, metabisulfite, formate or a combination thereof; and (b) optionally packaging as well as uses of such proline tolerant tripeptidyl peptidases, feed additive compositions, feed additive kits, feeds or feedstuffs and/or premixes.
摘要翻译：一种制备饲料添加剂组合物的方法，包括：（a）混合至少一种主要具有外肽酶活性的脯氨酸耐受性三肽肽酶，其中所述脯氨酸耐受性三肽肽酶能够从具有（i）（ A）P1处的脯氨酸; （B）选自丙氨酸，精氨酸，天冬酰胺，天冬氨酸，半胱氨酸，谷氨酰胺，谷氨酸，甘氨酸，组氨酸，异亮氨酸，亮氨酸，赖氨酸，甲硫氨酸，苯丙氨酸，丝氨酸，苏氨酸，色氨酸，酪氨酸，缬氨酸或合成的氨基酸 P1处的氨基酸; 或（ii）（a'）PV处的脯氨酸; 和（b'）选自丙氨酸，精氨酸，天冬酰胺，天冬氨酸，半胱氨酸，谷氨酰胺，谷氨酸，甘氨酸，组氨酸，异亮氨酸，亮氨酸，赖氨酸，甲硫氨酸，苯丙氨酸，丝氨酸，苏氨酸，色氨酸，酪氨酸，缬氨酸或 PV处的合成氨基酸或胺; 以及一种或多种选自盐，包括山梨醇和甘油的多元醇，小麦或小麦组分，乙酸钠，乙酸钠三水合物，山梨酸钾滑石，聚乙烯醇（PVA），苯甲酸酯，山梨酸酯，1,3 丙二醇，葡萄糖，对羟基苯甲酸酯，氯化钠，柠檬酸盐，偏亚硫酸氢盐，甲酸盐或其组合; 和（b）任选地包装以及这种耐受脯氨酸的三肽肽酶，饲料添加剂组合物，饲料添加剂药盒，饲料或饲料和/或预混合物的用途。

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