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1. 发明授权

EP2645848B1 STACKED HERBICIDE TOLERANCE EVENT 8264.44.06.1, RELATED TRANSGENIC SOYBEAN LINES, AND DETECTION THEREOF 有权转让
标题翻译：堆叠除草剂耐受事件8264.44.06.1，相关的转基因大豆系及其检测
公开(公告)号：EP2645848B1
公开(公告)日：2018-05-16
申请号：EP11845484.2
申请日：2011-12-02
申请人： Dow AgroSciences LLC , M.S. Technologies LLC
发明人： CUI, Yunxing , HOFFMAN, Thom , ZHOU, Ning , NOVAK, Stephen, N. , COLON, Julissa , PARKHURST, Dawn , TOLEDO, Sandra , WRIGHT, Terry , RUSSELL, Sean , HELD, Bruce , SEKAR, Vaithilingam
IPC分类号： C12N15/82 , C12N9/00 , C12N9/10 , C12Q1/68
CPC分类号： A01N39/04 , A01H1/02 , C12N9/1092 , C12N9/93 , C12N15/8241 , C12N15/8274 , C12N15/8275 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/16 , C12Y205/01019 , C12Y604/01002
摘要： This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

2. 发明授权

EP2525649B1 EXCISION OF TRANSGENES IN GENETICALLY MODIFIED ORGANISMS 有权转让
公开(公告)号：EP2525649B1
公开(公告)日：2020-01-08
申请号：EP11735267.4
申请日：2011-01-21
申请人： Dow AgroSciences, LLC
发明人： RUSSELL, Sean , PETOLINO, Joseph F.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/29 , C12N9/22

3. 发明公开

EP3382028A1 STACKED HERBICIDE TOLERANCE EVENT 8264.44.06.1, RELATED TRANSGENIC SOYBEAN LINES, AND DETECTION THEREOF 审中-实审
公开(公告)号：EP3382028A1
公开(公告)日：2018-10-03
申请号：EP18172142.4
申请日：2011-12-02
申请人： Dow AgroSciences LLC , M.S. Technologies LLC
发明人： CUI, Yunxing , HOFFMAN, Thom , ZHOU, Ning , NOVAK, Stephen N. , PARKHURST, Dawn , TOLEDO, Sandra , WRIGHT, Terry , RUSSELL, Sean , HELD, Bruce , SEKAR, Vaithilingam , COLON, Julissa
IPC分类号： C12N15/82 , C12N9/00 , C12N9/10 , C12Q1/68
CPC分类号： A01N39/04 , A01H1/02 , C12N9/1092 , C12N9/93 , C12N15/8241 , C12N15/8274 , C12N15/8275 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/16 , C12Y205/01019 , C12Y604/01002
摘要： This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be "stacked" with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

4. 发明公开

EP2525649A2 EXCISION OF TRANSGENES IN GENETICALLY MODIFIED ORGANISMS 有权转让
标题翻译：转基因的切除转基因生物体
公开(公告)号：EP2525649A2
公开(公告)日：2012-11-28
申请号：EP11735267.4
申请日：2011-01-21
申请人： Dow AgroSciences, LLC
发明人： RUSSELL, Sean , PETOLINO, Joseph F.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/29
摘要： A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

5. 发明公开

EP3066915A1 STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY 无效
标题翻译： FÜRDIEERHÖHUNGEINER PFLANZENTRANSFORMATIONSFREQUENZ MODIFIZIERTE AGROBAKTERIUM-STÄMME
公开(公告)号：EP3066915A1
公开(公告)日：2016-09-14
申请号：EP16166288.7
申请日：2011-07-29
申请人： Dow AgroSciences LLC
发明人： MERLO, Donald J. , RETALLACK, Diane , RUSSELL, Sean , WOOSLEY, Aaaron , MEADE, Tom , NARVA, Kenneth E.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/84 , C12N1/21 , C12N15/74
CPC分类号： C12N15/8205 , C07K14/195 , C07K14/325 , C12N15/74 , C12N15/743 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.
摘要翻译：提供了在能够独立于农杆菌染色体，Ti质粒和植物转化双向载体复制的质粒上携带转化增强基因的土壤杆菌菌株，以及这些农杆菌菌株的用途。另外，导致DNA重组功能缺陷的农杆菌菌株导致植物转化双向载体的不稳定或重排，并且能够独立于农杆菌染色体，Ti质粒和植物转化二元体的能够复制的质粒上转录增强基因还提供了这些菌株的载体和用途。还包括农杆菌菌株，其在不干扰或以其他方式损害农杆菌细胞的正常生长和植物转化能力的位点处将整合到农杆菌染色体中的转化增强基因并用于这些农杆菌菌株。还描述了使用这些农杆菌菌株制备的植物。

6. 发明公开

EP2645848A1 STACKED HERBICIDE TOLERANCE EVENT 8264.44.06.1, RELATED TRANSGENIC SOYBEAN LINES, AND DETECTION THEREOF 有权转让
标题翻译： STAPELBARES HERBIZIDTOLERANTES活动8264.44.06.1，ENTSPRECHENDE TRANSGENE SOJABOHNENLINIEN UND NACHWEIS DAVON
公开(公告)号：EP2645848A1
公开(公告)日：2013-10-09
申请号：EP11845484.2
申请日：2011-12-02
申请人： Dow AgroSciences LLC , M.S. Technologies LLC
发明人： CUI, Yunxing , HOFFMAN, Thom , ZHOU, Ning , NOVAK, Stephen, N. , COLON, Julissa , PARKHURST, Dawn , TOLEDO, Sandra , WRIGHT, Terry , RUSSELL, Sean , HELD, Bruce , SEKAR, Vaithilingam
IPC分类号： A01H5/00 , C07H21/04
CPC分类号： A01N39/04 , A01H1/02 , C12N9/1092 , C12N9/93 , C12N15/8241 , C12N15/8274 , C12N15/8275 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/16 , C12Y205/01019 , C12Y604/01002
摘要： This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
摘要翻译：本发明部分涉及大豆事件pDAB8264.44.06.1，并且包括新的表达盒和转基因插入物，其包含赋予对草甘膦，芳氧基链烷酸酯和草铵膦除草剂的抗性的多种性状。本发明还部分涉及控制抗性杂草，植物育种和除草剂耐受性植物的方法。在一些实施方案中，事件序列可以与其他性状“堆叠”，包括例如其它除草剂耐受性基因和/或昆虫抑制蛋白。本发明还部分涉及用于检测大豆和相关植物材料中事件pDAB8264.44.06.1的终点TaqMan PCR测定。一些实施方案可以进行植物材料的高通量接合度分析，并且其它实施方案可用于唯一地鉴定包含本发明事件的大豆品系的接合度和品种。还提供了用于进行这些测定的试剂盒和条件。

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