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    • 7. 发明公开
    • DETECTION OF AAD-12 SOYBEAN EVENT 416
    • NACHWEIS DES AAD12-SOJABOHNEN-EREIGNISSES 416
    • EP2504456A4
    • 2014-04-09
    • EP10833901
    • 2010-11-24
    • DOW AGROSCIENCES LLC
    • NOVAK STEPHENCUI YUNXING CORYGREENE THOMAS WZHOU NING
    • C12Q1/68A01H1/04
    • C12N15/8274A01H1/04C12Q1/6895C12Q2600/16C12Q2600/166
    • The invention relates in part to methods of detecting an AAD-12 soybean event. The subject invention provides assays for detecting the presence of the subject event in a sample (of soybeans, for example). Kits and conditions useful in conducting the assays are also provided. More specifically, the present invention relates in part to an endpoint TaqMan PCR assay for the AAD-12 soybean event. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the discovery of a preferred reference gene for use in determining zygosity. This invention also relates in part to plant breeding using any of the subject methods. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits. The subject procedures can be used to uniquely identify soybean lines comprising the event of the subject invention.
    • 本发明部分涉及检测AAD-12大豆事件的方法。 本发明提供了用于检测样品(例如大豆)的受试者事件的存在的测定。 还提供了用于进行测定的试剂盒和条件。 更具体地,本发明部分涉及AAD-12大豆事件的终点TaqMan PCR测定。 一些实施方案涉及能够进行高通量接合度分析的测定。 本发明还部分涉及用于确定接合性的优选参考基因的发现。 本发明还部分地涉及使用任何本发明方法的植物育种。 在一些实施方案中,所述事件/多核苷酸序列可以与其他特征“堆叠”。 本发明方法可用于唯一地鉴定包含本发明事件的大豆品系。