专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

EP1271133B1 Method of measurement based on fluorescence energy transfer using a long-lived fluorescence donor 有权
标题翻译：基于荧光能量转移长与供体荧光寿命测量方法
公开(公告)号：EP1271133B1
公开(公告)日：2008-05-28
申请号：EP01908141.3
申请日：2001-02-28
申请人： DAIICHI PURE CHEMICALS CO., LTD. , Nagano, Tetsuo
发明人： NAGANO, Tetsuo , KIKUCHI, Kazuya , KORESAWA, Mitsunori , KOJIMA, Hirotatsu
IPC分类号： G01N21/78 , G01N33/50 , C09K11/07
CPC分类号： G01N33/542 , G01N21/6428 , G01N2021/6441
摘要： A method for measuring a substance to be measured by means of a fluorescence, which comprises carrying out a measurement in the presence of (1) a specific fluorescent probe emitting a fluorescence by a specific reaction with the substance to be measured and (2) a donor having a long life fluorescence by itself and being capable of combining with the specific fluorescent probe as an acceptor and of allowing the acceptor to undergo a fluorescence resonance energy transfer (provided that the donor forms no chemical bonding with the specific fluorescent probe), wherein a fluorescence emitted by the reaction of the acceptor with the substance to be measured is converted to a long life fluorescence of excitation type by the fluorescence resonance energy transfer induced in the acceptor.

2. 发明公开

EP1271133A1 MEASURING METHOD USING LONG LIFE FLUORESCENCE OF EXCITATION TYPE 有权
标题翻译： Messverfahren basierend auf Fluoreszenz- Energie-Transfer mit einem Donor langer Fluoreszenzlebenszeit
公开(公告)号：EP1271133A1
公开(公告)日：2003-01-02
申请号：EP01908141.3
申请日：2001-02-28
申请人： DAIICHI PURE CHEMICALS CO. LTD. , Nagano, Tetsuo
发明人： NAGANO, Tetsuo , KIKUCHI, Kazuya , KORESAWA, Mitsunori , KOJIMA, Hirotatsu
IPC分类号： G01N21/78
CPC分类号： G01N33/542 , G01N21/6428 , G01N2021/6441
摘要： A method for measurement of a target substance in a sample by means of fluorescence, which comprises the step of carrying out the measurement in the presence of (1) a specific fluorescent probe which specifically reacts with the target substance to generate fluorescence, and (2) a donor which, per se, has long-lived fluorescence and is capable of inducing fluorescence resonance energy transfer to the specific fluorescent probe that acts as an acceptor, provided that the donor forms no binding to the specific fluorescent probe by means of a chemical bond; and the step of converting fluorescence, wherein said fluorescence is resulted from a reaction between the acceptor and the target substance, into long-lived excitation fluorescence by the fluorescence resonance energy transfer which is induced on the acceptor.
摘要翻译：一种通过荧光测定样品中的目标物质的方法，其包括在（1）特异性荧光探针存在下进行测定的步骤的步骤，所述荧光探针与目标物质特异性反应以产生荧光，和（2 ）供体本身具有长寿命的荧光并且能够诱导荧光共振能量转移到用作受体的特异性荧光探针，条件是供体通过化学物质不与特定荧光探针结合键; 以及通过在受体上诱导的荧光共振能量转移，将受体和目标物质之间的反应所产生的荧光发生长期激发荧光的荧光转化步骤。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式