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1. 发明公开

EP0336736A1 Purification of pertussis toxins and production of vaccine 失效
标题翻译： Reinigung von Pertussis-Toxinen和Herstellung von Vakzine。
公开(公告)号：EP0336736A1
公开(公告)日：1989-10-11
申请号：EP89303369.6
申请日：1989-04-05
申请人： CONNAUGHT LABORATORIES LIMITED
发明人： Tan, Larry U. L. , Jackson, Gail , Alkema, Dirk , Wah, Po S. , Fahim, Rafaat Emil Fahmy
IPC分类号： A61K39/10 , C07K3/18
CPC分类号： C07K14/235 , A61K39/00
摘要： Lymphocytosis promoting factor (LPF) and filamentous hemaglutinin (FHA) are isolated from the growth medium of the Bordatella pertussis organism and purified by selectively adsorbing the LPF and FHA on a selective adsorbing medium, such as filter aids or gel filtration media, at low ionic strength and subsequently removing the adsorbed LPF and FHA using an aqueous medium of high ionic strength, either simultaneously or sequentially. Prior to desorption of the LPF and FHA, the adsorbing medium may be contacted with an aqueous solution of an non-ionic detergent, which enables the LPF and FHA subsequently desorbed to be substantially free from contamination by lipopolysaccharides (LPS). The LPF and FHA may be further purified on hydroxyapatite. The LPF and FHA may be detoxified separately or together by contacting with a cross-linking agent, such as glutaraldehyde or formaldehyde, in the presence of an anti-aggregation agent. The resulting purified and detoxified LPF and FHA may be used to formulate a vaccine against pertussis.
摘要翻译：淋巴细胞增多促进因子（LPF）和丝状血凝素（FHA）从百日咳杆菌生物培养基中分离，并通过选择性吸附LPF和FHA在选择性吸附介质如过滤助剂或凝胶过滤介质上，在低离子并且随后使用高离子强度的水性介质同时或顺序地除去吸附的LPF和FHA。在LPF和FHA的解吸附之前，吸附介质可以与非离子型洗涤剂的水溶液接触，这使得LPF和FHA随后解吸基本上不受脂多糖（LPS）的污染。可以在羟基磷灰石上进一步纯化LPF和FHA。 LPF和FHA可以在抗聚集剂存在下，通过与交联剂如戊二醛或甲醛接触来单独或一起解毒。所得到的纯化和解毒的LPF和FHA可用于配制针对百日咳的疫苗。

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