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1. 发明授权

EP1165748B1 APPAREIL ET PROCEDE DE LYSE PAR ULTRASONS DE CELLULES BIOLOGIQUES 有权
标题翻译：用于超声破坏生物细胞的装置和方法
公开(公告)号：EP1165748B1
公开(公告)日：2004-06-02
申请号：EP00915279.4
申请日：2000-04-03
申请人： Biomerieux S.A.
发明人： COLIN, Bruno , CLEUZIAT, Philippe , BROYER, Patrick , MABILAT, Claude , INCARDONA, Sandra
IPC分类号： C12M1/33 , C12M3/08
CPC分类号： B01J19/10 , B06B3/00 , C12M45/02

2. 发明公开

EP1305398A1 PROCEDE DE LYSE CELLULAIRE DE PROCARYOTES OU D'EUCARYOTES OU DE LYSE CELLULAIRE SIMULTANEE DE PROCARYOTES ET D'EUCARYOTES 有权
标题翻译：方法原核生物或真核生物的细胞裂解或原核生物和真核生物同时细胞裂解
公开(公告)号：EP1305398A1
公开(公告)日：2003-05-02
申请号：EP01958181.8
申请日：2001-07-26
申请人： Biomerieux S.A.
发明人： CLEUZIAT, Philippe , INCARDONA, Sandra , JAY, Corinne
IPC分类号： C12M3/08
CPC分类号： C12N1/06 , C12M47/06
摘要： The invention concerns a method for cellular lysis of prokaryotes or eukaryotes or simultaneous cellular lysis of prokaryotes and eukaryotes. It consists in adapting at least the following three parameters: a percentage by mass of active beads with small diameter relative to active beads with large diameter not more than 50 %, and/or a total mass of lysing beads, comprising or not a mixture of small diameter beads and/or large diameter beads, ranging between 50 and 100 % relative to the total mass of the treated biological sample, and/or a lysis duration ranging between 10 and 20 minutes, and/or a number of non-lysing glass beads but driving lysing beads less than seven (7), and/or a number of non-lysing iron beads but driving lysing beads ranging between five (5) and fifteen (15), based on the sonication, mechanical vortex, or magnetic vortex technique. The invention is in particular applicable in the field of cellular lysis.

3. 发明公开

EP1242633A1 NUCLEIC ACID AMPLIFICATION AND DETECTION OF MYCOBACTERIUM SPECIES 失效
标题翻译：核酸扩增和分枝杆菌的检测
公开(公告)号：EP1242633A1
公开(公告)日：2002-09-25
申请号：EP99968145.5
申请日：1999-12-17
申请人： Gen-Probe Incorporated , Biomerieux S.A.
发明人： BRENTANO, Steven, T. , JUCKER, Markus, T. , DELGADO, Francisco, D. , CLEUZIAT, Philippe , RODRIGUE, Marc
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.

4. 发明公开

EP1165748A1 APPAREIL ET PROCEDE DE LYSE PAR ULTRASONS DE CELLULES BIOLOGIQUES 有权
标题翻译：用于提供超声波和细胞生物学技术的装置
公开(公告)号：EP1165748A1
公开(公告)日：2002-01-02
申请号：EP00915279.4
申请日：2000-04-03
申请人： Biomerieux S.A.
发明人： COLIN, Bruno , CLEUZIAT, Philippe , BROYER, Patrick , MABILAT, Claude , INCARDONA, Sandra
IPC分类号： C12M1/33 , C12M3/08
CPC分类号： B01J19/10 , B06B3/00 , C12M45/02
摘要： The invention concerns an apparatus comprising a sonotrode (2) designed to generate more or less high power ultrasounds in at least a biological sample (5) containing cells to be lysed, which sample is contained in at least a receptacle (4 or 10) suited for that purpose. The invention also concerns an apparatus comprising at least two sonotrodes and an ultrasonic lysis method of a biological sample (5) contained in a receptacle (4), using at least such a sonotrode (2). The apparatus is characterised in that the sonotrode (2) is directly in contact with the receptacle(s) (4). The method consists in: positioning the receptacle (4) in direct contact with the sonotrode (2); activating said sonotrode (2) for a time interval sufficient to lyse the cells in the sample (5) while preserving the released DNA's and/or the RNA's for subsequent use, for example in a step of amplification. The invention is preferably applicable in the field of molecular biology.
摘要翻译：本发明涉及一种包括超声波发生器（2）的设备，所述超声波发生器（2）被设计为至少在含有待裂解细胞的生物样品（5）中产生或多或少的高功率超声波，所述样品包含在至少一个容器为了这个目的。本发明还涉及使用至少一个超声波发生器（2）的装置，其包含至少两个超声波发生器和包含在容器（4）中的生物样品（5）的超声波裂解方法。该设备的特征在于超声波发生器（2）直接与容器（4）接触。该方法包括：将容器（4）定位成与超声波发生器（2）直接接触; 例如在扩增步骤中，激活所述超声波发生器（2）足够的时间间隔以裂解样品（5）中的细胞，同时保留释放的DNA和/或RNA以供随后使用。本发明优选适用于分子生物学领域。

5. 发明授权

EP1242633B1 NUCLEIC ACID AMPLIFICATION AND DETECTION OF MYCOBACTERIUM SPECIES 失效
标题翻译： NUKLEINSÄUREAMPLIFIZIERUNGDE DEVICE VON MYKOBACTERIUM-SPEZIES
公开(公告)号：EP1242633B1
公开(公告)日：2011-01-12
申请号：EP99968145.5
申请日：1999-12-17
申请人： GEN-PROBE INCORPORATED , Biomerieux S.A.
发明人： BRENTANO, Steven, T. , JUCKER, Markus, T. , DELGADO, Francisco, D. , CLEUZIAT, Philippe , RODRIGUE, Marc
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.
摘要翻译：公开了用于引发16S rRNA序列的体外核酸扩增的寡核苷酸或编码16S rRNA序列的DNA，用于许多属于分枝杆菌属的物种。公开了包括这种寡核苷酸的试剂盒。公开了使用寡核苷酸在体外核酸扩增中检测分枝杆菌的方法。

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