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1. 发明公开

EP3377224A1 MICROFLUIDIC DEVICE POSSESSING STRUCTURES ENABLING DIFFERENTIAL ANALYSIS OF A SINGLE CELL'S CONSTITUENTS 审中-公开
公开(公告)号：EP3377224A1
公开(公告)日：2018-09-26
申请号：EP16798123.2
申请日：2016-11-15
申请人： Koninklijke Philips N.V. , Danmarks Tekniske Universitet
发明人： VAN DER ZAAG, Pieter, Jan , MARIE, Rodolphe, Charly, Willy , VAN STRIJP, Dianne, Arnoldina, Margaretha, W. , OLESEN, Tom , VULDERS, Roland, Cornelis, Martinus , KRISTENSEN, Anders
IPC分类号： B01L3/00
CPC分类号： B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/06 , B01L2400/0622 , C12M47/06 , C12N15/1003
摘要： A method and a micro fluidic device comprising at least one micro fluidic structure for differential extraction of nuclear and extra-nuclear constituents of a single cell, said micro fluidic structure comprising a feeding channel for receiving a volume of a sample containing at least one cell, at least one trapping structure for capturing a single cell, and at least one output channel in fluid connection with the at least one trapping structure, wherein the at least one trapping structure extends from one side of the feeding channel substantially perpendicular to longitudinal axis of the feeding channel, the at least one trapping structure possessing an aperture at its end opposite to the fluid channel and in fluid communication with an output channel, said aperture being configured to provide a narrow section such that the nucleus of a cell captured in the trapping structure cannot pass through said narrow section into the output channel.

2. 发明授权

EP3162885B1 ULTRAHIGH-PRESSURE HOMOGENIZING INTEGRATED DEVICE AND CELL DISRUPTOR 有权
公开(公告)号：EP3162885B1
公开(公告)日：2018-07-04
申请号：EP15840792.4
申请日：2015-03-31
申请人： Guang Zhou Juneng Nano&Bio Technology Co., Ltd
发明人： YU, Xingwen , YU, Qian
IPC分类号： C12M1/33 , C12M1/02 , C12M1/00 , C12N1/06 , F04B9/113
CPC分类号： C12M47/06 , C12M23/34 , C12M27/00 , C12M29/14 , C12M33/12 , C12M41/40 , C12M45/02 , C12M47/08 , F04B9/113 , F04B31/00
摘要： Disclosed is an ultrahigh-pressure homogenizing integrated device comprising a long oil cylinder, a main connecting sleeve, a high-pressure cylinder homogenizing main body, an auxiliary connecting sleeve and a short oil cylinder, wherein the upper part of the high-pressure cylinder homogenizing main body is provided with a feeding hole communicated with a high-pressure cavity; and the feeding hole is connected with an integrated feeding device. The long oil cylinder, the main connecting sleeve, the high-pressure cylinder homogenizing main body, the auxiliary connecting sleeve and the short oil cylinder are sequentially and coaxially arranged. A pressurizing plunger rod in the high-pressure cavity of the high-pressure cylinder homogenizing main body is connected with a piston rod of the long oil cylinder; and a homogenizing valve arranged in the inner cavity, which is communicated with the high-pressure cavity, of the high-pressure cylinder homogenizing main body, is connected with an ejector rod of the short oil cylinder. The invention also discloses a cell disruptor. The ultrahigh-pressure homogenizing integrated device and cell disruptor of the present invention are stable in operation, small in space occupation, compact in structure, allow stop and running under high pressure and allow non-load running, are invariable in temperature, have less connecting tubes, less procedures and are not easy to leak.

3. 发明公开

EP3222988A4 SPECIMEN FRAGMENTATION DEVICE AND METHOD FOR SAME 审中-公开
公开(公告)号：EP3222988A4
公开(公告)日：2017-11-22
申请号：EP15860310
申请日：2015-11-17
申请人： WAKO PURE CHEM IND LTD , FUJIFILM TECHNO PRODUCTS CO LTD
发明人： YONEDA AKITO , KAWABATA TOMOHISA , ENDO YOICHI , HIBE DAISUKE , TSUYUKI MIKIO , DENAWA TATSUYUKI
IPC分类号： G01N1/28 , B01F9/00 , B01F13/00 , C12M1/00
CPC分类号： C12Q1/6806 , B01F9/0005 , B01F9/002 , B01F13/0052 , B02C17/06 , B02C17/08 , B02C25/00 , C12M1/00 , C12M47/06 , G01N1/28 , G01N1/286 , G01N2001/2866
摘要： [Objective] A specimen is efficiently disrupted within a short period of time, in a specimen disrupting apparatus and a specimen disrupting method that rotate a container having the specimen, a great number of small diameter beads, and a large diameter bead stored therein. [Constitution] A solution (S) that includes a specimen, a great number of small diameter beads (SB), and a large diameter bead (BB) are stored in a container (2). A drive unit (40) rotates the lower portion of the container (2) having the above components stored therein. A control unit (70) controls the drive unit (40) such that the lower portion of the container (2) is rotated at two or more different rotational speeds which change continuously.

4. 发明公开

EP3194535A1 SYSTEM AND METHOD FOR PROCESSING BIOMASS 有权
标题翻译：用于处理生物质的系统和方法
公开(公告)号：EP3194535A1
公开(公告)日：2017-07-26
申请号：EP15842636.1
申请日：2015-09-15
申请人： Salonen, Petteri
发明人： Salonen, Petteri
IPC分类号： C10J3/86 , C02F3/00 , C02F11/00 , C12M1/107
CPC分类号： C12M47/14 , C02F1/025 , C02F9/00 , C02F11/04 , C02F11/12 , C02F11/123 , C02F11/125 , C02F11/185 , C02F2303/10 , C05F5/008 , C12M21/04 , C12M21/12 , C12M35/02 , C12M43/04 , C12M43/08 , C12M45/06 , C12M45/20 , C12M47/06 , C12M47/20 , Y02A40/212 , Y02E50/343 , Y02P20/129 , Y02P20/145 , Y02P20/59 , Y02W10/30 , Y02W10/37 , Y02W30/47
摘要： System (100) and method for processing biomass. The system comprises a combined heat and power plant (102), an interface (114) for feeding biogas to a traffic fuel production unit, interfaces (114) to a district heating system (106a) and an electrical grid (106b), and a hydrolysis device (108), a digestion device (110), a dryer (116) and a heat recovery unit (112), which are operatively coupled for transferring heat, intermediate products and final products of the process, wherein raw biomass is received into the Fuel hydrolysis device (108), biomass processed by the hydrolysis device (108) is fed to the digestion device (110), biogas obtained in the digestion device (110) is fed to the traffic fuel production unit (104), heat is recovered from the hydrolysis device (108), biomass processed by the digestion device (110) is dried by the heat recovered from the hydrolysis device (108), heat is recovered from the dryer (116), heat recovered from the dryer (116) is fed to the hydrolysis device (108) to be used in pre-heating of the received raw biomass, heat recovered from the dryer (116) is fed to the district heating (106a), and production of electricity is fueled by the dried biomass from the dryer (116).
摘要翻译：系统（100）和用于处理生物质的方法。该系统包括联合的热电厂（102），用于将沼气馈送到交通燃料生产单元的接口（114），与区域供热系统（106a）和电网（106b）的接口（114），以及水解装置（108），消化装置（110），干燥器（116）和热回收单元（112），其被操作性地联接以传递过程的热量，中间产物和最终产物，其中将原料生物质接收水解装置108，由水解装置108处理的生物质被供给到消化装置110，在消化装置110中获得的生物气体被供给到交通燃料生成单元104，来自水解装置（108）的由消化装置（110）处理的生物质被从水解装置（108）回收的热量干燥，从干燥器（116）回收热量，从干燥器（116）回收的热量为进料到水解装置（108）以用于预加热所接收的物质将从干燥器116回收的热量供应到区域供热106a，并且通过来自干燥器116的干燥生物质来供应电力。

5. 发明公开

EP3172311A1 METHOD AND APPARATUS FOR DISRUPTION OF BIOMASS CELLS 审中-公开
标题翻译：用于破坏生物量细胞的方法和设备
公开(公告)号：EP3172311A1
公开(公告)日：2017-05-31
申请号：EP15741224.8
申请日：2015-07-24
申请人： VITO NV (Vlaamse Instelling voor Technologisch Onderzoek NV)
发明人： D'HONDT, Els , ELST, Kathy , GÜNERKEN, Emre , GARCIA-GONZALEZ, Linsey , WIJFFELS, René H. , EPPINK, Michel H.M.
IPC分类号： C12M1/04 , C12M1/06 , C12M1/26 , C12N1/06
CPC分类号： C12N1/066 , C12M47/06
摘要： The present invention relates to a method for disrupting suspended biomass material comprising the steps of: - providing a suspension of biomass material in a first vessel (1), said suspension having a dry cell weight being comprised between 0.1% and 15% (w/w); - pressurizing said suspension to a pressure (p1) being comprised between 7500 kPa and 450000 kPa by using a first high-pressure pump (10); - providing a pressurized gas disruption agent and/or a pressurized liquid disruption agent in a second vessel (2); - pressurizing said gas and/or liquid disruption agent to a pressure (p2) being comprised between 7500 kPa and 450000 kPa by using a second high-pressure pump (20), said pressure (p2) being higher than said pressure (p1); - injecting a stream of said pressurized disruption agent into a stream of said pressurized suspension of biomass material by using a sparger system (7), thereby bringing said two streams together and forming a micro-emulsion; - passing said micro-emulsion sequentially through a hydrodynamic agitation system (11) and a relief valve (13). An apparatus (and stack of (parallel) systems) for disrupting suspended biomass material and use thereof is provided as well.
摘要翻译：本发明涉及一种破坏悬浮的生物质材料的方法，该方法包括以下步骤： - 提供生物质材料在第一容器（1）中的悬浮液，所述悬浮液的干细胞重量为0.1％至15％（w / W）; - 通过使用第一高压泵（10）将所述悬浮液加压至包括在7500kPa和450000kPa之间的压力（p1）; - 在第二容器（2）中提供加压气体破坏剂和/或加压液体破坏剂; - 通过使用第二高压泵（20）将所述气体和/或液体破坏剂加压至7500kPa至450000kPa之间的压力（p2），所述压力（p2）高于所述压力（p1）;以及 - - 通过使用鼓泡系统（7）将所述加压破坏剂的物流注入所述生物质材料的加压悬浮液的物流中，从而将所述两股物流合并在一起并形成微乳液; - 使所述微乳液依次通过流体动力学搅拌系统（11）和减压阀（13）。还提供了用于破坏悬浮的生物质材料的装置（和（并联）系统的堆叠）及其用途。

6. 发明公开

EP3160650A1 ARBRE D'AGITATION POUR BROYEUR 有权转让
公开(公告)号：EP3160650A1
公开(公告)日：2017-05-03
申请号：EP15753079.1
申请日：2015-06-23
申请人： Roquette Frères
发明人： SEGARD, David , PATINIER, Samuel
IPC分类号： B02C17/16 , B02C17/18
CPC分类号： B02C17/163 , B08B3/102 , B08B9/0817 , C12M27/02 , C12M47/06
摘要： An agitator shaft (1) for a grinding mill, comprising: - an axle (2) intended to be driven in rotation, - a succession of flat agitator elements (3) and tubular spacers (4) mounted in alternation on the axle and in a stack along said axle (2), - end stops (5, 6), secured to the axle, that keep the stack of agitator elements and spacers under compression, characterized in that the tubular spacers (4) have orifices allowing the free circulation of material and fluids from the outside of the agitator shaft to the inside, and vice versa.
摘要翻译： 1。一种用于研磨机的搅拌器轴（1），包括： - 用于旋转驱动的轴（2）， - 在轴上交替安装的一系列平面搅拌器元件（3）和管状间隔器（4）沿着所述轴（2）的堆叠， - 固定到所述轴的端部止挡件（5,6），所述端部止挡件保持所述搅拌器元件和间隔件的堆叠处于压缩状态，其特征在于，所述管状间隔件（4）具有允许自由循环的材料和流体从搅拌器轴外部流向内部，反之亦然。

7. 发明授权

EP2232259B1 SYSTEM, APPARATUS AND METHOD FOR MATERIAL PREPARATION AND/OR HANDLING 有权转让
标题翻译：系统，设备和方法材料制备和/或处理
公开(公告)号：EP2232259B1
公开(公告)日：2016-10-12
申请号：EP09700618.3
申请日：2009-01-09
申请人： Keck Graduate Institute
发明人： DOEBLER, Robert , NADIM, Ali , STERLING, James, D. , HICKERSON, Anna , ERWIN, Barbara , WOYSKI, Denice
IPC分类号： C12N1/06 , G01N1/28
CPC分类号： C12M47/06 , C12M23/28 , C12M27/00 , C12N1/066 , G01N1/286 , G01N1/4077 , G01N2001/4083 , G01N2001/4088 , G01N2001/4094

8. 发明公开

EP3045524A1 CONTROLLED SYSTEM FOR SUPPORTING ALGAE GROWTH WITH ADSORBED CARBON DIOXIDE 审中-公开
标题翻译： GESTEUERTES SYSTEM ZURUNTERSTÜTZUNGDES ALGENWACHSTUMS MIT ADSORBIERTEM KOHLENSTOFFDIOXID
公开(公告)号：EP3045524A1
公开(公告)日：2016-07-20
申请号：EP16150793.4
申请日：2016-01-11
申请人： GENERAL ATOMICS
发明人： Ordway, David , Hazlebeck, David
IPC分类号： C12M1/00 , C12M1/34 , C12M1/36 , A01G33/00
CPC分类号： C12M21/02 , A01G33/00 , C12M21/12 , C12M23/18 , C12M23/40 , C12M29/06 , C12M29/24 , C12M41/34 , C12M41/48 , C12M43/02 , C12M43/06 , C12M47/02 , C12M47/06 , C12M47/18 , Y02A40/88
摘要： A system and method for creating a useful carbon-enriched media in a reactor which wall assimilate carbon into an algae biomass, requires measuring a respective carbon concentration of the media, C (measured) , as it enters, and as it leaves the reactor. Operationally, desired carbon concentration values are preset, C (set) , and are provided along with values obtained for C (measured) as input to a system controller. Respective differentials between C (measured) and C (set) at the reactor's input and output ports are determined by the controller and are used to control a volumetric fluid flow rate of the media through the reactor. Specifically, the controller establishes a volumetric fluid flow rate of the media as it is passed through an absorber where the media is carbon-enriched by interaction with combustion gases from an external source (e.g. a power plant).
摘要翻译：用于在将碳同化成藻类生物质的反应器中产生有用的富碳介质的系统和方法需要测量介质中的C（测量的）当它进入时和当其离开反应器时各自的碳浓度。在操作上，所需的碳浓度值被预设为C（设定），并且与作为输入的C（被测量）获得的值一起提供给系统控制器。反应器输入和输出端口的C（测量）和C（设定）之间的差异由控制器确定，用于控制通过反应器的介质的体积流体流速。具体地，控制器确定介质在通过吸收器时的体积流体流速，其中介质通过与来自外部源（例如发电厂）的燃烧气体的相互作用富集碳。

9. 发明公开

EP3030655A1 VERFAHREN UND VORRICHTUNG ZUM AUFBEREITEN EINER ZIELZELLEN UND BEGLEITZELLEN ENTHALTENDEN PROBE BIOLOGISCHEN MATERIALS ZUM EXTRAHIEREN VON NUKLEINSÄUREN DER ZIELZELLEN 审中-公开
标题翻译：方法和装置治疗靶细胞和随附细胞样品含有生物材料提取NUCLEIC靶细胞
公开(公告)号：EP3030655A1
公开(公告)日：2016-06-15
申请号：EP14744852.6
申请日：2014-07-30
申请人： Robert Bosch GmbH
发明人： FALTIN, Bernd , LAERMER, Franz , RUPP, Jochen , ROTHACHER, Peter , DORRER, Christian , BERNING, Sebastian
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1003 , C07H1/06 , C12M47/04 , C12M47/06 , C12N1/06 , C12N1/066 , C12N1/08 , C12N15/1017 , G01N29/00
摘要： The invention relates to a method (100) for preparing a sample of biological materials containing target cells and accompanying cells for extracting nucleic acids of the target cells. The method (100) comprises a step (110) of accumulating the target cells of the sample by separating the target cells or the accompanying cells from the sample. The method (100) also comprises a step (120) of digesting the target cells via chemical and/or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method (100) furthermore comprises a step (130) of purifying the nucleic acids from the target cell lysate in order to extract the nucleic acids of the target cells.
摘要翻译：一种用于制备生物材料含有靶细胞的样品，并伴随细胞用于提取靶细胞的核酸的方法包括：通过从所述样品中分离靶细胞或细胞伴随累积样品的靶细胞。因此，该方法包括消化通过化学和/或物理裂解靶细胞，以产生靶细胞裂解物含有靶细胞的核酸。该方法包括进一步净化从靶细胞裂解物的核酸，以便提取靶细胞的核酸。

10. 发明公开

EP2814939A4 NANOPORE SENSOR FOR ENZYME-MEDIATED PROTEIN TRANSLOCATION 有权转让
标题翻译： NANOPORENSENSORFÜRENZYMVERMITTELTE PROTEINTRANSLOKATION
公开(公告)号：EP2814939A4
公开(公告)日：2016-02-24
申请号：EP13749423
申请日：2013-02-15
申请人： UNIV CALIFORNIA
发明人： NIVALA JEFFREY M , MARKS DOUGLAS B , AKESON MARK A
IPC分类号： C12M1/00 , B82Y15/00 , C12Q1/25 , G01N27/403
CPC分类号： C12M47/06 , B82Y15/00 , C12Q1/48 , G01N33/48721 , G01N33/54366
摘要： Described herein is a device and method for translocating a protein through a nanopore and monitoring electronic changes caused by different amino acids in the protein. The device comprises a nanopore in a membrane, an amplifier for providing a voltage between the cis side and trans side of the membrane, and an NTP driven unfoldase which processed the protein to be translocated. The exemplified unfoldase is the ClpX unfoldase from E. coli.
摘要翻译：本文描述了通过纳米孔将蛋白质转位并监测由蛋白质中的不同氨基酸引起的电子变化的装置和方法。该装置包括膜中的纳米孔，用于在膜的顺侧和反面之间提供电压的放大器以及加工待移位的蛋白质的NTP驱动的非折叠酶。示例性的解折叠酶是来自大肠杆菌的ClpX解折叠酶。

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