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1. 发明公开

EP3192877A1 VESICULAR LINKER AND USES THEREOF IN NUCLEIC ACID LIBRARY CONSTRUCTION AND SEQUENCING 有权转让
标题翻译： VESICULAR LINKER及其在核酸库构建和测序中的应用
公开(公告)号：EP3192877A1
公开(公告)日：2017-07-19
申请号：EP14901593.5
申请日：2014-11-21
申请人： BGI Shenzhen Co., Limited
发明人： JIANG, Yuan , GUO, Jing , JI, Xiaojun , GENG, Chunyu , TIAN, Kai , ZHAO, Xia , XU, Huaiqian , ZHANG, Wenwei , JIANG, Hui , DRMANAC, Radoje
IPC分类号： C12Q1/68 , C12N15/10 , C40B50/06 , C40B40/08 , C12N15/66
CPC分类号： C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/06 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
摘要： Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.
摘要翻译：提供了通过使用接头构建的囊泡接头和单链环状库。该文库可用于RNA测序和依赖于单链循环文库的其他测序平台，并具有高通量测序，高准确度和简单操作的优点。

2. 发明公开

EP3225721A1 METHOD AND REAGENT FOR CONSTRUCTING NUCLEIC ACID DOUBLE-LINKER SINGLE-STRAND CYCLICAL LIBRARY 有权转让
标题翻译：构建核酸双链单链循环库的方法和试剂
公开(公告)号：EP3225721A1
公开(公告)日：2017-10-04
申请号：EP14907081.5
申请日：2014-11-26
申请人： BGI Shenzhen , BGI Shenzhen Co., Limited
发明人： JIANG, Yuan , ZHAO, Xia , ALEXEEV, Andrei , DRMANAC, Radoje , ZHANG, Wenwei , JIANG, Hui
IPC分类号： C40B50/06 , C40B50/18 , C12N15/11 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/1068 , C12N15/10 , C12N15/11 , C12Q1/68 , C40B50/06 , C40B50/18
摘要： A method and reagent for constructing a nucleic acid double-joint single-strand cyclical library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase is provided on a primer sequence; using USER enzyme to cleave the first product and cyclizing to produce a gap; or, a nicking enzyme recognition sequence is also provided on the primer sequence, using the USER enzyme to cleave the first product, cyclizing and using a nicking enzyme for nicking to produce a nick; performing a restrictive nick/gap translation reaction from the nick or the gap; removing by digestion any portion that did not undergo the restrictive nick/gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and using a mediated sequence for cyclization of a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments and obviates the need for gel extraction; the single-strand nucleic acid molecule can be cyclized directly when denatured with heat.
摘要翻译：构建核酸双联单链循环文库的方法和试剂。该方法包括：将核酸分解成核酸片段; 连接第一连接序列; 通过扩增产生在任一末端提供有第一接头序列的第一产物，其中在引物序列上提供U核碱基; 使用USER酶裂解第一种产物并环化以产生间隙; 或者，在引物序列上还提供切口酶识别序列，使用USER酶切割第一种产物，环化并使用切口酶进行切口以产生切口; 从缺口或间隙执行限制性缺口/缺口翻译反应; 通过消化除去未经历限制性切口/间隙翻译反应的任何部分; 连接第二连接序列; 通过扩增产生在任一端提供有第二接头序列的第二产物; 使第二种产物变性，并使用介导的序列使单链核酸分子环化。该方法允许增加文库插入片段的长度并且不需要凝胶提取; 单链核酸分子可以在加热变性时直接环化。

3. 发明公开

EP3207169A1 MATE PAIR LIBRARY CONSTRUCTION 有权转让
标题翻译：联机图书馆建设
公开(公告)号：EP3207169A1
公开(公告)日：2017-08-23
申请号：EP15850902.6
申请日：2015-10-13
申请人： BGI Shenzhen Co., Limited
发明人： JIANG, Yuan , DRMANAC, Radoje , HUROWITZ, Evan , ALEXEEV, Andrei , ZHAO, Xia , RUAN, Jie
IPC分类号： C40B50/06 , C40B40/06 , C12Q1/68
CPC分类号： C12N15/1093 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C40B40/06 , C40B50/06 , C12Q2521/501 , C12Q2525/191 , C12Q2565/50 , C12Q2565/631
摘要： The present invention provides a novel method for ligating an adapter to a target polynucleotide and methods of generating a library of mate-pair polynucleotide constructs that employ such a ligation method. Libraries and arrays comprising mate-pair polynucleotide constructs, and methods of sequencing libraries and arrays comprising mate-pair polynucleotide constructs, are also provided.
摘要翻译：本发明提供了用于将衔接子连接至靶多核苷酸的方法以及使用这种连接方法产生配对多核苷酸构建体的方法。还提供了包含配对多核苷酸构建体的文库和阵列以及包含配对多核苷酸构建体的测序文库和阵列的方法。

4. 发明公开

EP3208343A1 NUCLEIC ACID FRAGMENTATION METHOD AND SEQUENCE COMBINATION 有权转让
标题翻译：核酸碎片方法和序列组合
公开(公告)号：EP3208343A1
公开(公告)日：2017-08-23
申请号：EP14904045.3
申请日：2014-10-13
申请人： BGI Shenzhen Co., Limited
发明人： HAN, Hongyan , GENG, Chunyu , GUO, Guanying , ZHANG, Wenwei , JIANG, Hui , JIANG, Yuan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6806 , C12Q2525/179 , C12Q2525/191 , C12Q2535/122
摘要： Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5'-end extension primer, wherein the single-stranded 5'-end extension primer comprises a sequencing platform adaptor sequence of a 5' end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3'-end adaptor sequence to the 3' end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.
摘要翻译：公开了核酸片段化方法和序列组合。该方法包括以下步骤：通过使用单链5'-末端延伸引物对变性核酸进行退火和延伸反应，其中单链5'-末端延伸引物包含测序平台的 5'末端和连接的随机序列，随机序列在变性核酸的随机位点进行退火; 并将双链3'端衔接子序列与延伸反应中产生的核酸的3'端进行定向连接，并进行变性和纯化以获得在两端具有衔接子序列的片段化单链核酸。

5. 发明公开

EP3208336A1 LINKER ELEMENT AND METHOD OF USING SAME TO CONSTRUCT SEQUENCING LIBRARY 有权转让
标题翻译：关联元素及其使用方法构建测序库
公开(公告)号：EP3208336A1
公开(公告)日：2017-08-23
申请号：EP14904072.7
申请日：2014-10-14
申请人： BGI Shenzhen Co., Limited
发明人： JIANG, Yuan , GENG, Chunyu , ZHAO, Xia , FU, Shujin , HE, Lingyu , LI, Yaqiao , SU, Xiaoshan , WU, Fanzi , ZHANG, Wenwei , JIANG, Hui , ALEXEEV, Andrei , DRMANAC, Radoje
IPC分类号： C12N15/11 , C40B50/06 , C40B40/06 , C12Q1/68
CPC分类号： C12N15/1093 , C12N9/1205 , C12N9/1252 , C12N9/16 , C12N15/11 , C12Q1/68 , C12Q1/6806 , C12Q1/6855 , C12Q2525/191 , C12Y207/01078 , C12Y207/07007 , C12Y301/03001 , C40B40/06 , C40B50/06 , C12Q2521/531 , C12Q2525/155 , C12Q2525/186 , C12Q2525/307 , C12Q2535/122 , C12Q2537/162 , C12Q2563/131
摘要： Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3' end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3' end thereof can be in a complementary pairing with the 5' end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.
摘要翻译：提供了接头元件和使用接头元件构建测序文库的方法，其中接头元件由接头A和接头B组成，接头A通过长核酸链和长链核酸链的互补配对获得短链核酸链中，长链的5'末端具有磷酸修饰，短链的3'末端具有封闭修饰，在短链中具有酶位点; 并且接头B是核酸单链，并且其3'末端可以与接头A的长链的5'末端互补配对。使用本发明的接头元件构建测序文库保证了连接子的连接方向性，同时解决了片段连接，连接子自连和连接效率低的问题，减少了步骤间的纯化反应，缩短了连接时间，降低了成本。

6. 发明公开

EP3192901A1 METHOD FOR CONSTRUCTING NUCLEIC ACID SINGLE-STRANDED CYCLIC LIBRARY AND REAGENTS THEREOF 有权转让
标题翻译：构建核酸单链循环库的方法和试剂
公开(公告)号：EP3192901A1
公开(公告)日：2017-07-19
申请号：EP14901602.4
申请日：2014-11-26
申请人： BGI Shenzhen Co., Limited
发明人： GENG, Chunyu , CHEN, Ruoying , JIANG, Yuan , ZHAO, Xia , GUO, Rongrong , HE, Lingyu , LI, Yaqiao , ZHANG, Wenwei , JIANG, Hui , DRMANAC, Radoje
IPC分类号： C40B50/06 , C12Q1/68
CPC分类号： C12N15/1093 , C12N15/1089 , C12N15/66 , C12Q1/6806 , C12Q1/6853 , C12Q1/6855 , C12Q2525/191 , C12Q2521/307 , C12Q2521/507 , C12Q2525/121 , C12Q2525/307 , C12Q2563/131
摘要： Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.
摘要翻译：提供了构建核酸单链环状文库和其中使用的试剂的方法。该方法通过转座酶中断与限制性缺口翻译反应相结合，实现了简单快速的核酸单链循环文库构建。

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