专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP3395945A1 VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN ORGANISMEN 无效
公开(公告)号：EP3395945A1
公开(公告)日：2018-10-31
申请号：EP18155821.4
申请日：2004-07-16
申请人： BASF Plant Science GmbH
发明人： ZANK, Thorsten , BAUER, Joerg , CIRPUS, Petra , ABBADI, Amine , HEINZ, Ernst , QIU, Xiao , VRINTEN, Patricia , SPERLING, Petra , DOMERGUE, Frederic , MEYER, Astrid , KIRSCH, Jelena
IPC分类号： C12N9/02
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung von mehrfach ungesättigten Fettsäuren in einem Organismus, indem Nukleinsäuren in den Organismus eingebracht werden, die für Polypeptide mit Δ-5-Elongaseaktivität codieren. Vorteilhaft können diese Nukleinsäuresequenzen gegebenenfalls zusammen mit weiteren Nukleinsäuresequenzen, die für Polypeptide der Biosynthese des Fettsäure- oder Lipidstoffwechels codieren, in dem Organismus exprimiert werden. Besonders vorteilhaft sind Nukleinsäuresequenzen, die für eine Δ-6-Desaturase-, eine Δ-5-Desaturase-, Δ-4-Desaturase- und/oder Δ-6-Elongaseaktivität codieren. Vorteilhaft stammen diese Desaturasen und Elongasen aus Thalassiosira, Euglena oder Ostreococcus. Weiterhin betrifft die Erfindung ein Verfahren zur Herstellung von Ölen und/oder Triacylglyceriden mit einem erhöhten Gehalt an langkettigen mehrfach ungesättigten Fettsäuren.
Die vorliegende Erfindung betrifft außerdem in einer bevorzugten Ausführungsform ein Verfahren zur Herstellung von ungesättigten ω-3 Fettsäuren sowie ein Verfahren zur Herstellung von Triglyceriden mit einem erhöhten Gehalt an ungesättigten Fettsäuren, besonders von ω-3 Fettsäuren mit mehr als drei Doppelbindungen. Die Erfindung betrifft die Herstellung eines transgenen Organismus bevorzugt einer transgenen Pflanze oder eines transgenen Mikroorganismus mit erhöhtem Gehalt an ungesättigten ω-3-Fettsäuren, Ölen oder Lipiden mit ω-3-Doppelbindungen aufgrund der Expression der im erfindungsgemäßen Verfahren verwendeten Elongasen und Desaturasen vorteilhaft in Verbindung mit ω-3-Desaturasen z.B. einer ω-3-Desaturase aus Pilzen der Familie Pythiaceae wie der Gattung Phytophtora beispielsweise der Gattung und Art Phytophtora infestans oder einer ω-3-Desaturase aus Algen wie der Familie der Prasinophyceae z.B. der Gattung Ostreococcus speziell der Gattung und Art Ostreococcus tauri oder Diatomeen wie der Gattung Thalassiosira speziell der Gattung und Art Thalassiosira pseudonana.
Die Erfindung betrifft weiterhin die Nukleinsäuresequenzen, Nukleinsäurekonstrukte, Vektoren und Organismen enthaltend die erfindungsgemäßen Nukleinsäuresequenzen, Vektoren enthaltend die Nukleinsäuresequenzen und/oder die Nukleinsäurekonstrukte sowie transgene Organismen enthalten die vorgenannten Nukleinsäuresequenzen, Nukleinsäurekonstrukte und/oder Vektoren.
Ein weiterer Teil der Erfindung betrifft Öle, Lipide und/oder Fettsäuren hergestellt nach dem erfindungsgemäßen Verfahren und deren Verwendung. Außerdem betrifft die Erfindung ungesättigte Fettsäuren sowie Triglyceride mit einem erhöhten Gehalt an ungesättigten Fettsäuren und deren Verwendung.

2. 发明授权

EP1599582B1 VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN 有权
标题翻译：用于生产多不饱和脂肪酸
公开(公告)号：EP1599582B1
公开(公告)日：2011-07-27
申请号：EP04706146.0
申请日：2004-01-29
申请人： BASF Plant Science GmbH
发明人： RENZ, Andreas , HEINZ, Ernst , ABBADI, Amine , DOMERGUE, Frederic , ZANK, Thorsten
IPC分类号： C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism by introducing nucleic acids coding for polypeptides with acyl-CoA:lysophospholipid-acyl transferase activity into the organism. Advantageously, said nucleic acid sequences can optionally be expressed in the transgenic organism along with additional nucleic acid sequences coding for polypeptides of fatty acid metabolism or lipid metabolism biosynthesis. The invention also relates to the nucleic acid sequences, nucleic acid structures containing said nucleic acid sequences, vectors containing the inventive nucleic acid sequences, and/or the nucleic acid structures and transgenic organisms containing said nucleic acid sequences, nucleic acid structures, and/or vectors. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and the use thereof.

3. 发明公开

EP1613744A2 DELTA-4-DESATURASEN AUS EUGLENA GRACILIS, EXPRIMIERENDE PFLANZEN UND PUFA ENTHALTENDE ÖLE 有权
标题翻译：来自小眼虫Δ-4去饱和酶，表达植物和多不饱和脂肪酸含油
公开(公告)号：EP1613744A2
公开(公告)日：2006-01-11
申请号：EP04725899.1
申请日：2004-04-06
申请人： BASF Plant Science GmbH
发明人： CIRPUS, Petra , BAUER, Jörg , MEYER, Astrid , HEINZ, Ernst , ZÄHRINGER, Ulrich
IPC分类号： C12N9/02 , C12P7/64 , A01H1/00 , C12N15/00 , A01K67/00 , A23D9/00 , A23K1/16
CPC分类号： C12N9/0071 , C12N15/8247
摘要： The invention relates to an improved method for the specific production of unsaturated ω-3 fatty acids and a method for producing triglycerides containing an increased number of unsaturated fatty acids, especially ω-3 fatty acids comprising more than three double bonds. Disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism containing an increased number of fatty acids, oils, or lipids comprising Δ-4 double bonds as a result of a Δ-4 desaturase being expressed from Euglena gracilis. The invention further relates to expression cassettes containing a nucleic acid sequence, a vector, and organisms containing at least one nucleic acid sequence or an expression cassette. Also disclosed are unsaturated fatty acids and triglycerides having an increased fatty acid content and the use thereof. Fatty acids and triglycerides are used in a plurality of application in the food industry, in animal nutrition, cosmetics, and pharmaceuticals and are suitable for many different applications depending on whether free saturated or unsaturated fatty acids or triglycerides having an increased saturated or unsaturated fatty acid content are used.

4. 发明公开

EP1831377A1 VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN ORGANISMEN 审中-公开
标题翻译：过程在转基因生物PRODUCING多不饱和脂肪酸
公开(公告)号：EP1831377A1
公开(公告)日：2007-09-12
申请号：EP05819684.1
申请日：2005-12-21
申请人： BASF Plant Science GmbH
发明人： CIRPUS, Petra , BAUER, Jörg , HEINZ, Ernst , DOMERGUE, Frederic
IPC分类号： C12N15/82 , C12N9/02 , A01H5/00
CPC分类号： C12N9/0083 , C12N15/8247
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism by introducing into the organism nucleic acids which encode polypeptides having Δ-5-elongase, Δ-6-desaturase, Δ-5-desaturase, Δ-4-desaturase, Δ-12-desaturase and/or Δ-6-elongase activity. Preferably, these desaturases and elongases are derived from Ostreococcus. The invention also relates to a method for producing oils and/or triacylglycerides having an increased content in long-chain polyunsaturated fatty acids. The invention further relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the inventive nucleic acid sequences, to vectors comprising the nucleic acid sequences and/or to the nucleic acid constructs and to transgenic organisms comprising the aforementioned nucleic acid sequences, nucleic acid constructs and/or vectors. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method and to the use thereof. The invention finally relates to fatty acids and triglycerides having an increased content in unsaturated fatty acids and to the use thereof.

5. 发明授权

EP1181373B1 DELTA6-ACETYLENASE UND DELTA6-DESATURASE AUS CERATODON PURPUREUS 有权
标题翻译： DELTA6 acetylenase和DELTA6脱氢酶关角齿藓
公开(公告)号：EP1181373B1
公开(公告)日：2006-07-26
申请号：EP00945722.7
申请日：2000-06-07
申请人： BASF Plant Science GmbH
发明人： HEINZ, Ernst , STYMNE, Sten , LEE, Michael , GIRKE, Thomas , SPERLING, Petra , ZAEHRINGER, Ulrich
IPC分类号： C12N15/53 , C12N9/02 , C12N15/82 , C12N1/19 , C12P7/64 , C12Q1/68 , C11C1/00 , A01H5/00
CPC分类号： C12N9/0071 , C12N9/0083 , C12N15/8247
摘要： The invention relates to a method for producing unsaturated fatty acids and to a method for preparing triglycerides with an increased unsaturated fatty acid content. The invention also relates to the utilization of DNA sequences coding for Δ6-acetylenase/Δ6-desaturases or Δ6-desaturases for producing a transgenic organism, preferably a transgenic plant or a transgenic microorganism with an increased fatty acid, oil or lipid or content with Δ6-triple bonds and/or Δ6-double bonds.

6. 发明授权

EP1472357B1 VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN MITTELS EINES NEUEN ELONGASE-GENS 有权
标题翻译：用于生产新的基因ELONGASE多不饱和脂肪酸MEANS
公开(公告)号：EP1472357B1
公开(公告)日：2009-12-16
申请号：EP03704382.5
申请日：2003-01-13
申请人： BASF Plant Science GmbH
发明人： LERCHL, Jens , HEINZ, Ernst , ZANK, Thorsten
IPC分类号： C12N15/82
CPC分类号： C12P7/6427 , C12N9/1029 , C12N15/8247 , C12P7/6472

7. 发明公开

EP1599582A2 VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN 有权
标题翻译：用于生产多不饱和脂肪酸
公开(公告)号：EP1599582A2
公开(公告)日：2005-11-30
申请号：EP04706146.0
申请日：2004-01-29
申请人： BASF Plant Science GmbH
发明人： RENZ, Andreas , HEINZ, Ernst , ABBADI, Amine , DOMERGUE, Frederic , ZANK, Thorsten
IPC分类号： C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism by introducing nucleic acids coding for polypeptides with acyl-CoA:lysophospholipid-acyl transferase activity into the organism. Advantageously, said nucleic acid sequences can optionally be expressed in the transgenic organism along with additional nucleic acid sequences coding for polypeptides of fatty acid metabolism or lipid metabolism biosynthesis. The invention also relates to the nucleic acid sequences, nucleic acid structures containing said nucleic acid sequences, vectors containing the inventive nucleic acid sequences, and/or the nucleic acid structures and transgenic organisms containing said nucleic acid sequences, nucleic acid structures, and/or vectors. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and the use thereof.

8. 发明公开

EP1356056A2 VERFAHREN ZUR EXPRESSION VON BIOSYNTHESEGENEN IN PFLANZLICHEN SAMEN UNTER VERWENDUNG VON NEUEN MULTIPLEN EXPRESSIONSKONSTRUKTEN 审中-公开
标题翻译：为了表达蔬菜种子的生物合成基因的方法是在使用新的多表达构建
公开(公告)号：EP1356056A2
公开(公告)日：2003-10-29
申请号：EP02710015.5
申请日：2002-01-18
申请人： BASF Plant Science GmbH
发明人： LERCHL, Jens , DUWENIG, Elke , BISCHOFF, Friedrich , HEINZ, Ernst , DREXLER, Hjördis , SCHEFFLER, Jodi
IPC分类号： C12N15/53
CPC分类号： C12N9/0083 , C12N9/1029 , C12N15/8247
摘要： The invention relates to expression cassettes, combinations thereof and vectors containing said expression cassettes, containing plant promoters with an expression specificity for plant seeds, in particular linseed and the use of said expression cassettes or vectors for the recombinant expression of heterologous genes in plants. The invention further relates to transgenic plants, transformed by means of said expression cassettes, or vectors, cultures, parts or transgenic propagations derived therefrom and the use of the above as foodstuff, animal feedstuff, seedstuff, pharmaceuticals, fine chemicals or industrial raw material.

9. 发明公开

EP1181373A1 DELTA6-ACETYLENASE UND DELTA6-DESATURASE AUS CERATODON PURPUREUS 有权
标题翻译： DELTA6 acetylenase和DELTA6脱氢酶关角齿藓
公开(公告)号：EP1181373A1
公开(公告)日：2002-02-27
申请号：EP00945722.7
申请日：2000-06-07
申请人： BASF Plant Science GmbH
发明人： HEINZ, Ernst , STYMNE, Sten , LEE, Michael , GIRKE, Thomas , SPERLING, Petra , ZAEHRINGER, Ulrich
IPC分类号： C12N15/53 , C12N9/02 , C12N15/82 , C12N1/19 , C12P7/64 , C12Q1/68 , C11C1/00 , A01H5/00
CPC分类号： C12N9/0071 , C12N9/0083 , C12N15/8247
摘要： The invention relates to a method for producing unsaturated fatty acids and to a method for preparing triglycerides with an increased unsaturated fatty acid content. The invention also relates to the utilization of DNA sequences coding for Δ6-acetylenase/Δ6-desaturases or Δ6-desaturases for producing a transgenic organism, preferably a transgenic plant or a transgenic microorganism with an increased fatty acid, oil or lipid or content with Δ6-triple bonds and/or Δ6-double bonds.

10. 发明授权

EP1720988B1 VERFAHREN ZUR HERSTELLUNG VON UNGESÄTTIGTEN OMEGA-3-FETTSÄUREN IN TRANSGENEN ORGANISMEN 有权
标题翻译：用于生产不饱和ω-3脂肪酸的转基因生物
公开(公告)号：EP1720988B1
公开(公告)日：2011-10-12
申请号：EP05715463.5
申请日：2005-02-23
申请人： BASF Plant Science GmbH
发明人： CIRPUS, Petra , BAUER, Jörg , ZANK, Thorsten , HEINZ, Ernst
IPC分类号： C12N15/82 , C12P7/64 , C12N5/10 , C12N15/53 , C12N9/02
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The invention relates to a method for producing unsaturated O3 fatty acids, and to a method for producing triglycerides having an increased unsaturated fatty acid content, especially O3 fatty acids with more than three double bonds. The invention also relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic micro-organism, with an increased content of unsaturated O3 double bonds on the basis of the expression of a O3 desaturase of fungi of the pythiacae family, such as the species phytophtora, for example the species and type phytophtora infestans. The invention further relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing at least one inventive nucleic acid sequence, at least one vector containing the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms containing the cited nucleic acid sequences, nucleic acid constructs and/or vectors. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use thereof. Furthermore, the invention relates to unsaturated fatty acids and triglycerides having an increased unsaturated fatty acid content, and to the use of the same.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式