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    • 2. 发明公开
    • METHOD FOR PRODUCING CELL EXTRACT HAVING AGRICULTURAL PEST INSECTICIDAL CAPACITY AND METHOD FOR INSECTICIDE OF AGRICULTURAL PEST
    • 工艺生产用于农业杀虫病虫害防治细胞提取物和程序的农业害虫的杀虫剂
    • EP3106514A1
    • 2016-12-21
    • EP14881916.2
    • 2014-02-10
    • Towada Green Tuff Agro-Science Co., Ltd.Kinki University
    • ANO TakashiHIROSE Yoichiro
    • C12N1/14A01N63/04
    • A01N63/04C12N1/14C12N3/00C12P1/02
    • Provided are a method for producing a cell extract having agricultural pest insecticidal capacity by a novel method for culturing filamentous fungi having insecticidal capacity for an agricultural pest, and a method for insecticide having an exceptional immediate effect within a short period of time on an agricultural pest due to filamentous fungi contained in a cell extract produced by this production method. A method for producing a cell extract having agricultural pest insecticidal capacity provided with a step for culturing filamentous fungi in solid medium and obtaining a solid culture of the filamentous fungi, a step for culturing the filamentous fungi in liquid medium and obtaining a liquid culture of the filamentous fungi, a step for separating mycelia of the filamentous fungi from the liquid culture of the filamentous fungi, a step for mixing the liquid culture from which the mycelia have been removed and the solid culture and obtaining a mixed culture, and a step for extracting a spore solution of the filamentous fungi from this mixed culture; and a method for insecticide of an agricultural pest that causes a cell extract obtained by this production method to act on an agricultural pest or a habitat thereof.
    • 提供了用于制造具有用于培养具有用于在农业害虫的杀虫能力丝状真菌通过一种新颖的方法农业害虫的杀虫能力的细胞提取物的方法,以及用于具有出色的即时效果杀虫剂很短的时间周期内对农业害虫的方法 由于包含在通过该制造方法制造的细胞提取物丝状真菌。 一种生产具有设置有用于在固体培养基中培养丝状真菌和获得的丝状真菌中,用于培养在液体介质中的丝状真菌和获得的液体培养物的步骤的固体培养步骤农业害虫的杀虫能力的细胞提取物的方法 丝状真菌,对于从丝状真菌的液体培养物中分离的丝状真菌的菌丝体的步骤,用于混合从该菌丝体被去除的液体培养和固体培养和获得的混合培养物的步骤,以及用于提取步骤 从该混合培养丝状真菌的孢子溶液; 和用于农业害虫的杀虫剂的方法没有导致由该制造方法作用于农业害虫或其栖息地而获得的细胞提取物。
    • 4. 发明公开
    • METHOD FOR PRODUCING CELL EXTRACT HAVING AGRICULTURAL PEST INSECTICIDAL CAPACITY AND METHOD FOR INSECTICIDE OF AGRICULTURAL PEST
    • VERFAHREN ZUR ERZEUGUNG VON ZELLEXTRAKT MIT INSEKTIZIDER WIRKUNG ZUR LANDWIRTSCHAFTLICHESCHÄDLINGSBEKÄMPFUNGUND VERFAHRENFÜRLANDWIRTSCHAFTLICHESSCHÄDLINGSINSEKTIZID
    • EP3106514A4
    • 2017-09-06
    • EP14881916
    • 2014-02-10
    • TOWADA GREEN TUFF AGRO-SCIENCE CO LTDUNIV KINKI
    • ANO TAKASHIHIROSE YOICHIRO
    • A01N63/04A01P3/00C12N1/14C12N3/00C12P1/02
    • A01N63/04C12N1/14C12N3/00C12P1/02
    • Provided are a method for producing a cell extract having agricultural pest insecticidal capacity by a novel method for culturing filamentous fungi having insecticidal capacity for an agricultural pest, and a method for insecticide having an exceptional immediate effect within a short period of time on an agricultural pest due to filamentous fungi contained in a cell extract produced by this production method. A method for producing a cell extract having agricultural pest insecticidal capacity provided with a step for culturing filamentous fungi in solid medium and obtaining a solid culture of the filamentous fungi, a step for culturing the filamentous fungi in liquid medium and obtaining a liquid culture of the filamentous fungi, a step for separating mycelia of the filamentous fungi from the liquid culture of the filamentous fungi, a step for mixing the liquid culture from which the mycelia have been removed and the solid culture and obtaining a mixed culture, and a step for extracting a spore solution of the filamentous fungi from this mixed culture; and a method for insecticide of an agricultural pest that causes a cell extract obtained by this production method to act on an agricultural pest or a habitat thereof.
    • 本发明提供一种通过培养具有杀虫能力的农业害虫的丝状真菌的新型方法来制造具有农业害虫杀虫能力的细胞提取物的方法以及在短时间内对农业害虫具有特异即时效果的杀虫剂的方法 由于该生产方法产生的细胞提取物中含有丝状真菌。 一种生产具有农业害虫杀虫能力的细胞提取物的方法,该方法具有在固体培养基中培养丝状真菌并获得丝状真菌的固体培养物的步骤,在液体培养基中培养丝状真菌并获得 丝状菌,从丝状菌的液体培养物中分离丝状菌的菌丝体的工序,将去除了菌体的液体培养基与固体培养物混合,得到混合培养物的工序,以及提取工序 来自该混合培养物的丝状真菌的孢子溶液; 以及使通过该制造方法得到的细胞提取物作用于农业害虫或其栖息地的农业害虫的杀虫剂的方法。