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2. 发明公开

EP2808399A1 Soluble analyte detection and amplification 有权
标题翻译： Nachweis undVerstärkunglöslicher分析
公开(公告)号：EP2808399A1
公开(公告)日：2014-12-03
申请号：EP14171566.4
申请日：2004-08-31
申请人： Restalyst Pte Ltd
发明人： Lawton, Robert L
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , Y10S435/971 , Y10S435/973 , C12Q2563/173 , C12Q2563/179
摘要： The present invention relates to a method of detection of a compound of interest that is present at low levels in a sample. In particular, the present invention relates to a method of detection of a compound of interest in solution by a nucleic acid-labelled binding construct, separation of the unbound nucleic acid-labelled binding construct, and the detection of the bound nucleic acid-labelled binding construct in the solution phase. The present invention is particularly adaptable to be used in conjunction with a nucleic acid amplification reaction for detecting the presence or absence of the nucleic acid portion of binding construct in a sample indicating the presence or absence of the compound of interest.
摘要翻译：本发明涉及在样本中以低水平存在的目标化合物的检测方法。特别地，本发明涉及通过核酸标记的结合构建体检测溶液中感兴趣的化合物的方法，分离未结合的核酸标记的结合构建体以及检测结合的核酸标记的结合在溶液相中构建。本发明特别适合与核酸扩增反应结合使用，用于检测在表明存在或不存在感兴趣化合物的样品中结合构建体的核酸部分的存在或不存在。

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