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1. 发明公开

EP4299720A1 HUMAN FATTY LIVER MODEL CELLS TO BE USED IN SCREENING METHOD 审中-实审
公开(公告)号：EP4299720A1
公开(公告)日：2024-01-03
申请号：EP22759695.4
申请日：2022-02-24
申请人： PhoenixBio Co., Ltd.
发明人： TAKAHASHI, Masaki , KAKUNI, Masakazu , YOSHIKAWA, Nami
IPC分类号： C12N5/071 , C12Q1/02
摘要： An object of the present invention is to provide human fatty-liver model cells exhibiting symptoms of fatty-liver tissue that can be used for a method for screening for a substance having an effect on dyslipidemia including fatty liver and the present invention provides human fatty-liver model cells for use in a method for screening for a substance having an effect on dyslipidemia based on an increase/decrease of very low density lipoprotein (VLDL) in culture supernatant as an index, in which the culture supernatant of the cells contains VLDL and low density lipoprotein (LDL), in which the VLDL is contained more than the LDL.

2. 发明公开

EP3141112A4 HYPERURICEMIA MODEL 审中-公开
公开(公告)号：EP3141112A4
公开(公告)日：2018-01-17
申请号：EP15789821
申请日：2015-05-07
申请人： PHOENIXBIO CO LTD
发明人： KAKUNI MASAKAZU , IWASAKI YUMIKO , MUKAIDANI CHISE
IPC分类号： A01K67/027 , C12N5/077 , G01N33/15 , G01N33/50
CPC分类号： A01K67/0271 , A01K67/027 , A01K2207/12 , A01K2207/15 , A01K2227/105 , A01K2267/03 , G01N33/15 , G01N33/50 , G01N33/5088 , G01N2500/00
摘要： Provided is a non-human animal that is highly practical as a hyperuricemia model, the non-human animal being the following: (a) a non-human animal obtained by producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction; and subsequently administering a purine base-containing substance to the primary chimeric non-human animal, or (b) a non-human animal obtained by producing a serially transplanted chimeric non-human animal via two steps, a first step being a step of producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction, a second step being a step of transplanting the human hepatocytes grown in the body of the primary chimeric non-human animal to an immunodeficient non-human animal with liver dysfunction, the second step being performed one or more times; and subsequently administering a purine base-containing substance to the serially transplanted chimeric non-human animal.

3. 发明公开

EP3141112A1 HYPERURICEMIA MODEL 审中-公开
标题翻译：高血压模型
公开(公告)号：EP3141112A1
公开(公告)日：2017-03-15
申请号：EP15789821.4
申请日：2015-05-07
申请人： Phoenixbio Co., Ltd.
发明人： KAKUNI, Masakazu , IWASAKI, Yumiko , MUKAIDANI, Chise
IPC分类号： A01K67/027 , G01N33/15 , G01N33/50 , C12N5/077
CPC分类号： A01K67/0271 , A01K67/027 , A01K2207/12 , A01K2207/15 , A01K2227/105 , A01K2267/03 , G01N33/15 , G01N33/50 , G01N33/5088 , G01N2500/00
摘要： Provided is a non-human animal that is highly practical as a hyperuricemia model, the non-human animal being the following:
(a) a non-human animal obtained by producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction; and subsequently administering a purine base-containing substance to the primary chimeric non-human animal, or
(b) a non-human animal obtained by producing a serially transplanted chimeric non-human animal via two steps, a first step being a step of producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction, a second step being a step of transplanting the human hepatocytes grown in the body of the primary chimeric non-human animal to an immunodeficient non-human animal with liver dysfunction, the second step being performed one or more times; and subsequently administering a purine base-containing substance to the serially transplanted chimeric non-human animal.
摘要翻译：本发明提供一种作为高尿酸血症模型非常实用的非人动物，所述非人动物如下：（a）通过将人肝细胞移植至人肝细胞产生初级嵌合非人动物而获得的非人动物具有肝功能障碍的免疫缺陷非人动物; 随后向初级嵌合非人动物施用含嘌呤碱基的物质，或（b）通过两步产生连续移植的嵌合非人动物获得的非人动物，第一步是产生通过将人肝细胞移植到具有肝功能障碍的免疫缺陷的非人动物，第二步是将在初级嵌合非人动物体内生长的人肝细胞移植到免疫缺陷非人动物 - 具有肝功能障碍的人类动物，第二步进行一次或多次; 随后将嘌呤碱基物质施用于连续移植的嵌合非人动物。

4. 发明公开

EP2767160A4 CHIMERIC NON-HUMAN ANIMAL CARRYING HUMAN HEPATOCYTE 有权
标题翻译：与肝细胞不是人类嵌合体动物人
公开(公告)号：EP2767160A4
公开(公告)日：2015-07-01
申请号：EP12840420
申请日：2012-10-12
申请人： PHOENIXBIO CO LTD , NAT UNIV CORP TOTTORI UNIV
发明人： OSHIMURA MITSUO , KAZUKI YASUHIRO , MUKAIDANI CHISE , SHIMADA TAKASHI , KAKUNI MASAKAZU , HAMAMURA SATOKO , KAMIMURA HIDETAKA , KAWAMURA AKIO , NAKADA NAOYUKI , OHBUCHI MASATO , KATO KOTA
IPC分类号： A01K67/027 , G01N33/15 , G01N33/50
CPC分类号： A01K67/0271 , A01K2207/12 , A01K2217/075 , A01K2227/105 , A01K2267/03 , C12N9/0042 , G01N33/5067 , G01N33/5088

5. 发明公开

EP1950284A1 METHOD FOR CULTIVATION OF HAIR FOLLICULAR DERMAL SHEATH CELL 有权
标题翻译：毛囊毛囊鞘细胞的培养方法
公开(公告)号：EP1950284A1
公开(公告)日：2008-07-30
申请号：EP06811076.6
申请日：2006-10-02
申请人： Phoenixbio Co., Ltd. , BIOINTEGRENCE INC.
发明人： TOYOSHIMA, Koei , MATSUNAGA, Mikaru , YOSHIZATO, Katsutoshi
IPC分类号： C12N5/06 , A61L27/00
CPC分类号： C12N5/0627 , C12N2501/115 , C12N2501/135
摘要： A method for culture of hair follicular dermal sheath cells or precursor cells thereof which are potent cellular materials for such as hair regeneration by cell transplantation is provided. That is, by performing culture in an animal cell culture medium supplemented with platelet-derived growth factor AA (PDGF-AA) and fibroblast growth factor 2 (FGF2), hair follicular dermal sheath cells are proliferated while sustaining their function, or hair follicular dermal sheath precursor cells are differentiated into dermal sheath cells and proliferated.
摘要翻译：提供了一种用于培养毛囊皮肤细胞或其前体细胞的方法，所述毛囊皮肤细胞或其前体细胞是用于诸如通过细胞移植进行毛发再生的有效细胞材料。即，通过在补充有血小板衍生生长因子AA（PDGF-AA）和成纤维细胞生长因子2（FGF2）的动物细胞培养基中进行培养，毛囊皮肤鞘细胞在维持其功能的同时增殖，或者毛囊皮肤鞘前体细胞分化成真皮鞘细胞并增殖。

6. 发明公开

EP1816208A4 METHOD OF SCREENING CANDIDATE FOR DRUG 审中-公开
标题翻译：一种筛选ACTIVE候选人的
公开(公告)号：EP1816208A4
公开(公告)日：2007-12-26
申请号：EP05809323
申请日：2005-11-16
申请人： HIROSHIMA IND PROMOTION ORG , BIOINTEGRENCE INC , PHOENIXBIO CO LTD , SUMIKA CHEM ANALYSIS SERVICES
发明人： MUKAIDANI CHISE , YOSHIZATO KATSUTOSHI , NISHIKURA YASUFUMI , HORIE TORU , NAKAZAWA HIROSHI , KANAMARU HIROSHI , UEDA CHIAKI , HINE CHIEMI
IPC分类号： G01N33/50
CPC分类号： G01N33/5088 , G01N33/5067 , G01N2333/80
摘要： It is intended to provide a method of screening a candidate for a drug which is metabolized mainly by drug metabolizing enzymes CYP2D6, CYP2C9 and CYP2C19 with the use of a chimeric mouse carrying human hepatocytes transplanted thereinto, whereby it is judged whether or not a candidate for the drug is metabolized in a subject deficient in CYP2D6, CYP2C9 or CYP2C19. It is also intended to provide a method of judging whether or not a candidate for a drug induces or inhibits the activity of any one of drug metabolizing enzymes CYP1A2, CYP2C9, CYP2C19, CYP2D6 and CYP3A4 with the use of a chimeric mouse carrying human hepatocytes transplanted thereinto.

7. 发明公开

EP2842417A4 UROKINASE-TYPE PLASMINOGEN ACTIVATOR TRANSGENIC MOUSE 有权
标题翻译：转运尿素酶 - 促胰岛激素
公开(公告)号：EP2842417A4
公开(公告)日：2016-01-06
申请号：EP13780731
申请日：2013-04-25
申请人： TOKYO METROPOLITAN INST MEDICAL SCIENCE , CHUGAI PHARMACEUTICAL CO LTD , PHOENIXBIO CO LTD
发明人： KOHARA MICHINORI , JISHAGE KOICHI , KAWASE YOSUKE , MUKAIDANI CHISE , OSHITA HIROKI , HAMAMURA SATOKO
IPC分类号： A01K67/027 , C12N15/09 , G01N33/15 , G01N33/50
CPC分类号： A01K67/0275 , A01K67/0271 , A01K2207/12 , A01K2207/15 , A01K2217/052 , A01K2217/15 , A01K2217/206 , A01K2227/105 , A01K2267/0337 , A01K2267/035 , C12N9/6462 , C12N15/8509 , C12N15/8775 , C12N2015/8581 , G01N33/5067 , G01N33/5088 , Y10T436/146666
摘要： The present invention provides a mouse with liver damage, having a high degree of damage against the mouse's original hepatocytes while having a uPA gene in a heterozygous form, and a method for efficiently preparing the mouse. Specifically, the method for preparing a mouse with liver damage having the uPA gene in a heterozygous form comprises the following steps of: (i) transforming mouse ES cells with a DNA fragment containing a liver-specific promoter/enhancer and cDNA that encodes a urokinase-type plasminogen activator operably linked under the control thereof; (ii) injecting the transformed mouse ES cells obtained in step (i) into a host embryo; (iii) transplanting the host embryo obtained in step (ii) via the injection of the ES cells into the uterus of a surrogate mother mouse, so as to obtain a chimeric mouse; and (iv) crossing the chimeric mice obtained in step (iii), so as to obtain a transgenic mouse in which the DNA fragment is introduced in a heterozygous form.
摘要翻译：本发明提供了具有肝损伤的小鼠，其具有对小鼠原始肝细胞的高度损伤，同时具有杂合形式的uPA基因，以及有效制备小鼠的方法。具体而言，制备具有uPA基因的肝损伤的小鼠具有杂合形式的方法包括以下步骤：（i）用含有肝特异性启动子/增强子的DNA片段和编码尿激酶的cDNA转化小鼠ES细胞在其控制下可操作地连接的纤溶酶原激活剂; （ii）将步骤（i）中获得的转化的小鼠ES细胞注射到宿主胚胎中; （iii）通过将ES细胞注射到替代母亲小鼠的子宫中移植步骤（ii）中获得的宿主胚胎，从而获得嵌合小鼠; 和（iv）与步骤（iii）中获得的嵌合小鼠杂交，以获得其中DNA片段以杂合形式引入的转基因小鼠。

8. 发明公开

EP2471917A4 POLYNUCLEOTIDE DERIVED FROM NOVEL HEPATITIS C VIRUS STRAIN AND USE THEREOF 审中-公开
标题翻译：从一个新的丙型肝炎病毒株获得多核苷酸及其用途
公开(公告)号：EP2471917A4
公开(公告)日：2013-03-13
申请号：EP10811924
申请日：2010-08-25
申请人： TOKYO METROPOLITAN INST MEDICAL SCIENCE , PHOENIXBIO CO LTD
发明人： KOHARA MICHINORI , ARAI MASAAKI , MUKAIDANI CHISE
IPC分类号： C12N15/09 , A01K67/027 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N7/00 , C12Q1/02 , G01N33/15 , G01N33/50
CPC分类号： C12Q1/707 , C12N7/00 , C12N2770/24221 , C12N2770/24222 , C12N2770/24243 , C12N2770/24251 , G01N2333/18 , G01N2500/10
摘要： A polynucleotide encoding the amino acid shown in SEQ ID NO:2 or SEQ ID NO: 5, or encoding an amino acid sequence having not less than 98% identity thereto; preferably a polynucleotide comprising replacement of the amino acid corresponding to glutamic acid at position 1202 of SEQ ID NO:2 (position 177 of SEQ ID NO:5) with glycine, replacement of the amino acid corresponding to glutamic acid at position 1056 (position 31 of SEQ ID NO:5) with valine, and replacement of the amino acid corresponding to alanine at position 2199 (position 1174 of SEQ ID NO:5) with threonine.

9. 发明授权

EP1702632B1 HAIR GROWTH METHOD 有权
标题翻译： HAARWUCHSMETHODE
公开(公告)号：EP1702632B1
公开(公告)日：2012-10-31
申请号：EP04819977.2
申请日：2004-12-03
申请人： BIOINTEGRENCE INC. , Phoenixbio Co., Ltd.
发明人： YOSHIZATO, Katsutoshi , SHIMADA, Takashi , TOYOSHIMA, Koei , MATSUNAGA, Mikaru
IPC分类号： A61L27/36 , A61L27/38 , A61F2/10
CPC分类号： A61L27/3869 , A61F2/10 , A61K35/12 , A61L27/362 , A61L27/3666 , A61L27/3813 , A61L2430/18 , C12N5/0627 , C12N2501/115
摘要： A method of enabling effective hair growth and furthermore, inducing hair growth closely similar to the natural hair state in the case of transplanting dermal papillae or cultured dermal papilla cells into the skin to regenerate the hair, characterized by comprising transplanting a composition containing the following components into an epidermis defect site: (a) dermal papillae or dermal papilla cells, (b) an epidermal tissue or epidermal cells and/or (c) a tissue constituting hair follicles or cells thereof.

10. 发明授权

EP1950284B1 METHOD FOR CULTIVATION OF HAIR FOLLICULAR DERMAL SHEATH CELL 有权
标题翻译：毛囊毛囊鞘细胞的培养方法
公开(公告)号：EP1950284B1
公开(公告)日：2012-04-25
申请号：EP06811076.6
申请日：2006-10-02
申请人： Phoenixbio Co., Ltd. , BIOINTEGRENCE INC.
发明人： TOYOSHIMA, Koei , MATSUNAGA, Mikaru , YOSHIZATO, Katsutoshi
IPC分类号： C12N5/071
CPC分类号： C12N5/0627 , C12N2501/115 , C12N2501/135
摘要： Disclosed is a method for cultivation of a hair follicular dermal sheath cell or a precursor cell thereof which is a potential cellular material for hair regeneration by cell transplantation. The method comprises the step of cultivating a hair follicular dermal sheath cell or a precursor cell thereof in an animal cell culture medium supplemented with a platelet-derived growth factor AA (PDGF-AA) and a fibroblast growth factor 2 (FGF2). As a result, the hair follicular dermal sheath cell can be proliferated while maintaining its functions, or the precursor cell can be differentiated into a hair follicular dermal sheath cell and proliferated.
摘要翻译：本发明公开了一种培养毛囊皮肤细胞或其前体细胞的方法，所述毛囊皮肤细胞或其前体细胞是通过细胞移植作为毛发再生的潜在细胞材料。该方法包括在补充有血小板衍生生长因子AA（PDGF-AA）和成纤维细胞生长因子2（FGF2）的动物细胞培养基中培养毛囊皮肤真皮细胞或其前体细胞的步骤。结果，毛囊真皮鞘细胞可以在维持其功能的同时增殖，或者前体细胞可以分化成毛囊皮肤鞘细胞并增殖。

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