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1. 发明公开

EP1485701A1 ELECTROPHORETIC SEPARATION SYSTEM 审中-公开
标题翻译：电泳分离系统
公开(公告)号：EP1485701A1
公开(公告)日：2004-12-15
申请号：EP03742597.2
申请日：2003-02-12
申请人： Nextgen Sciences Ltd
发明人： AUTON, Kevin, Andrew , SCHUROV, Sergei, Mikhailovich , RYAN, Paul, Thomas
IPC分类号： G01N27/447
CPC分类号： G01N27/44773 , G01N27/44708 , G01N27/4476
摘要： An electrophoresis device for use in separating a mixture of analytes in a fluid sample is provided, the device comprising a first dimension separation medium (102) through which the analytes may migrate, the separation medium being carried on one face of a flexible sheet (101), the surface area of that face being greater than that of the region of contact between the separation medium and the sheet, wherein the first dimension separation medium is located within a first separation zone which is defined at least partly by the flexible sheet, the device further comprising a fluid chamber (105), separated from the first dimension separation zone by the flexible sheet (101), in which fluid may be retained in contact with that face of the sheet opposite to the face on which the separation medium is carried. The device may additionally comprise a second separation zone (108), in which a second separation medium may be located, in order to carry out a second dimension electrophoretic separation.
摘要翻译：提供了用于分离流体样品中分析物混合物的电泳装置，该装置包括第一维度分离介质（102），分析物可通过该第一维度分离介质迁移，分离介质承载在柔性片材（101）的一个面上），该面的表面积大于分离介质与片材之间接触区域的表面积，其中第一维度分离介质位于至少部分由柔性片材限定的第一分离区域内，装置，其还包括通过柔性片材（101）与第一尺寸分离区域隔开的流体室（105），其中流体可保持与片材的与携带分离介质的面相对的面。该装置可以另外包括第二分离区（108），其中可以放置第二分离介质，以便执行第二维电泳分离。

2. 发明公开

EP1379864A1 ELECTROPHORETIC SEPARATION SYSTEM 审中-公开
标题翻译：电泳分离系统
公开(公告)号：EP1379864A1
公开(公告)日：2004-01-14
申请号：EP02720223.3
申请日：2002-04-16
申请人： Nextgen Sciences Ltd
发明人： AUTON, Kevin Andrew , RYAN, Paul Thomas
IPC分类号： G01N27/447
CPC分类号： G01N27/44773
摘要： Electrophoretic separation device comprising first and second separation zones for containing first and second separation media, and barrier means, preferably automatically operable, by which fluid communication between the two zones may be reversibly prevented, the barrier means comprising a sealing element which is deformable between two positions to allow or prevent the fluid communication. The sealing element may comprise a flexible sheet carrying the first separation medium. Also provided are apparatus comprising such a separation device, and methods of electrophoretic separation, in one of which a first dimension separation is carried out in a chamber in the absence of any other separation medium, followed by introduction into the same chamber of a second separation medium, adjacent or in contact with the first, the analytes being allowed to migrate from the first to the second separation medium prior to conducting the second dimension separation.

3. 发明公开

EP1379644A2 PROTEIN ANALYSIS BY MEANS OF IMMOBILIZED ARRAYS OF ANTIGENS OR ANTIBODIES 审中-公开
标题翻译：蛋白质由固定的蛋白质安排的说明抗原或抗体分析
公开(公告)号：EP1379644A2
公开(公告)日：2004-01-14
申请号：EP02714342.9
申请日：2002-04-04
申请人： Nextgen Sciences Ltd
发明人： Auton, Kevin Andrew
IPC分类号： C12N15/10 , C12N15/62 , G01N33/533 , C12Q1/68
CPC分类号： C40B30/04 , C07K14/315 , C07K17/00 , C07K2319/00 , C07K2319/21 , C07K2319/23 , C12N15/62 , G01N33/6842 , G01N33/6845
摘要： A method of forming an array of proteins selected from antigens or antibodies; said method comprising the steps of (i) expressing in a recombinant cell, a fusion protein which ocmprises either (a) an antigen or (b) an antibody binding protein, fused to a peptide having up to 50 amino acids, which peptide comprises amino acid sequence of SEQ ID NO 1 LX1X2IX3X4X5X6KX7X8X9X10 (SEQ ID NO 1) where X1 is a naturally occurring amino acid, X2 is any naturally occurring amino acid other than leucine, valine, isoleucine, tryptophan, phenylalanine or tyrosine, X3 is phenylalanine or leucine, X4 is glutamine or asparagine, X5 is alanine, glycine, serine or threonine, X6 is glycine or methionine, X7 is isoleucine, methionine or valine, X8 is glutamine, leucine, valine, tyrosine or isoleucine, X9 is tryptophan, tyrosine, valine, phenylalanine, leucine or isoleucine and X10 is any naturally occurring amino acid other than asparagine or glutamine; where said peptide is capable of being biotinylated by a biotin ligase at the lysine residue adjacent to X6; (ii) biotinylating said peptide of the fusion protein at the lysine residue adjacent X6; (iii) isolating the biotinylated fusion protein; (iv) applying the biotinylated fusion protein to an avidin or streptavidin coated non-porous support; (v) forming an array of at least three different proteins on the support by either (a) where the fusion protein comprises an antigen, carrying out steps (i) to (iv) the desired number of times to form an antigen array; or (b) where the fusion protein comprises an antibody binding protein, applying to said protein, either prior to or after step (iv) a plurality of different antibodies or binding fragments thereof.

4. 发明公开

EP1345697A2 MICROFLUIDIC DEVICES AND METHODS FOR CHEMICAL ASSAYS 有权
标题翻译：微流体设备和方法化学样品
公开(公告)号：EP1345697A2
公开(公告)日：2003-09-24
申请号：EP01997351.0
申请日：2001-11-22
申请人： Nextgen Sciences Ltd
发明人： RYAN, Paul Thomas , AUTON, Kevin Andrew , SCHUROV, Sergei Mikhailovich , WIGLEY, David John
IPC分类号： B01L3/00
CPC分类号： F16K99/0001 , B01F11/0071 , B01F13/0059 , B01J2219/00286 , B01J2219/00353 , B01J2219/00389 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , B01J2219/00725 , B01L3/50273 , B01L3/502738 , B01L9/527 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , B01L2300/0822 , B01L2300/0874 , B01L2300/0887 , B01L2300/1805 , B01L2300/1827 , B01L2300/1838 , B01L2300/1844 , B01L2400/0481 , B01L2400/0487 , B01L2400/0638 , F16K7/12 , F16K99/0015 , F16K99/0034 , F16K99/0059 , F16K2099/0084 , G01F1/708 , G01F1/7086 , G01F1/7088 , G01F11/086 , G01P13/0006 , Y10T436/117497
摘要： An assay device in which to carry out a fluid-phase chemical assay, comprising means for supporting a test substrate, a sample chamber for receiving a fluid sample and at least one fluid control device for controlling the movement of fluid into and/or out of the sample chamber, wherein the fluid control device comprises a fluid outlet chamber in fluid communication with the sample chamber, and a displaceable flexible diaphragm the displacement of which alters the volume of the outlet chamber so as to allow and/or restrict fluid flow between the outlet and sample chambers. The invention also provides assay apparatus incorporating such a device, an assay station for use as part of such apparatus, a fluid control unit for use as part of the assay device and a method of conducting an assay which may involve the use of such apparatus and devices.

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