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1. 发明授权

EP0892067B1 FLUORESCENT ENZYME SUBSTRATES AND METHOD FOR ASSAYING ENZYMATIC ACTIVITY 失效
标题翻译：荧光酶底物和方法用于测量酶活性
公开(公告)号：EP0892067B1
公开(公告)日：2002-03-13
申请号：EP97947958.1
申请日：1997-12-16
申请人： Laboratory of Molecular Biophotonics
发明人： SHIONO, Hirofumi, Lab. Molecular Biophotonics , NOHTA, Hitoshi, Lab. Molecular Biophotonics , UTSUYAMA, Chika, Lab. Molecular Biophotonics
IPC分类号： C12Q1/42 , C12Q1/44 , C12Q1/34 , C12Q1/54
CPC分类号： C12Q1/34 , C12Q2334/00 , C12Q2334/20

2. 发明公开

EP0867506A1 SOLID PHASE FOR TARGET NUCLEIC ACID DETECTION, PROCESS FOR PRODUCTION THEREOF, AND METHOD OF TARGET NUCLEIC ACID DETECTION 失效
标题翻译：固相的靶核酸，方法生产的检测，检测方法
公开(公告)号：EP0867506A1
公开(公告)日：1998-09-30
申请号：EP97940369.8
申请日：1997-09-12
申请人： Laboratory of Molecular Biophotonics
发明人： ABE, Satoshi, Laboratory of Molecular Biophotonics , SATO, Yoshihiro
IPC分类号： C12N15/11 , C12N15/10 , C12Q1/68 , G01N33/53 , G01N33/566
CPC分类号： C12Q1/6834 , C12Q2537/143 , C12Q2533/107 , C12Q2525/307
摘要： A solid phase for target nucleic acid detection which has a base sequence hybridizable in sequence with a specified polynucleotide sequence of a target nucleic acid and contains a set of probes with their spatial arrangement immobilized onto the solid phase through a linker portion so as to be enzymatically ligated during the hybridization; and a method of target nucleic acid detection which comprises hybridizing the solid phase with the target nucleic acid, ligating the probes by a ligase reaction, and detecting the product of ligation.
摘要翻译：固相对于靶核酸检测其具有与靶核酸的特定多核苷酸序列的序列的碱基序列杂交并含有一组探针与他们的固定到固相的空间布置的通过左部，以便被酶在杂交期间结扎; 并且其包括与杂交靶核酸的固相，通过连接酶反应结扎探针，和检测结扎的产品目标核酸检测的方法。

3. 发明公开

EP1016731A1 PROBES FOR DETECTING TARGET NUCLEIC ACID, METHOD OF DETECTING TARGET NUCLEIC ACID, AND SOLID PHASE FOR DETECTING TARGET NUCLEIC ACID AND PROCESS FOR PRODUCING THE SAME 审中-公开
标题翻译：探针 - 靶核酸的测定，测定方法的靶核酸，固相的靶核酸和制造方法的测定
公开(公告)号：EP1016731A1
公开(公告)日：2000-07-05
申请号：EP99900152.2
申请日：1999-01-08
申请人： Laboratory of Molecular Biophotonics
发明人： ABE, Satoshi, Laboratory of Molecular Biophotonics , KODAMA, Hirofumi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6862 , C12Q1/6823 , C12Q1/6834 , C12Q2565/543 , C12Q2521/501 , C12Q2523/107 , C12Q2521/101
摘要： This invention relates to a pair of probes for the detection of a target nucleic acid, said pair of probes comprising (1) probe 1 having base sequence B1 complementary to A1 between two specific sequential base sequences A1 and A2 of the nucleic acid, wherein (a) the 5'-terminus of base sequence B1 is phosphorylated and (b) a first solid phase immobilizing part is bound to the 3'-terminus of base sequence B1, and (2) probe 2 having base sequence B2 complementary to A2 between base sequences A1 and A2, wherein (a) the 5'-terminus of base sequence B2 is bound to one end of a cleavage part and (b) a second solid phase immobilizing part is bound to the other part of the cleavage part. When the target nucleic acid is hybridized above the solid phase of the invention where said probes are immobilized on its surface, the probes on the solid phase occupy spatial positions beneficial to the formation of a hybrid and thus forms the hybrid efficiently. Therefore, probe 1 and probe 2 are efficiently ligated by ligase reaction. Furthermore, after the target nucleic acid is removed from the hybrid, only probe 2 ligated as described above will be able to exist on the solid phase through cleavage reaction of the cleavage part. Therefore, after the free probe 2 has been removed by washing, it will become possible to detect the presence of probe 2 on the solid phase ligated as described above, with high sensitivity and high recognition. This will enable detection of the presence of a target nucleic acid with higher sensitivity and higher recognition as compared to methods in the prior art.
摘要翻译：本发明涉及到一对探针的用于检测靶核酸的，所述一对探针，其包括核酸，worin的两个特定顺序的碱基序列A1和A2之间的（1）探针具有1碱基序列A1互补B1的（一个）的碱基序列B1的5'末端被磷酸化和（b）第一固相固定部分结合到碱基序列B1的3'末端，和（2）样品2具有碱基序列B2互补A2之间碱基序列A1和A2，worin的（a）碱基序列B2的5“末端结合到切割部分的一端和（b）第二固相固定部分结合到切割部分的另一部分。当靶核酸，其中所述探针在其表面上固定化本发明的固相以上的杂交，在固相上探针占据的杂交体的形成有利的空间位置，从而有效地形成混合。因此，探针1和探针2被有效地通过连接酶反应连接。进一步，在靶后核酸从混合去除，只有两个测试连接如上所述将能够通过切割部分的切割反应在固相存在。因此，免费试用2已经通过洗涤除去后，将成为能够检测上如上述那样，具有高灵敏度和高识别连接在固相样品2的存在。这将使一个靶核酸与更高的灵敏度和更高的识别的存在的检测相比，在现有技术中的方法。

4. 发明公开

EP0892067A1 FLUORESCENT ENZYME SUBSTRATES AND METHOD FOR ASSAYING ENZYMATIC ACTIVITY 失效
标题翻译：荧光素酶酵母菌素酵母菌
公开(公告)号：EP0892067A1
公开(公告)日：1999-01-20
申请号：EP97947958.1
申请日：1997-12-16
申请人： Laboratory of Molecular Biophotonics
发明人： SHIONO, Hirofumi, Lab. Molecular Biophotonics , NOHTA, Hitoshi, Lab. Molecular Biophotonics , UTSUYAMA, Chika, Lab. Molecular Biophotonics
IPC分类号： C12Q1/42 , C12Q1/44 , C12Q1/34 , C12Q1/54
CPC分类号： C12Q1/34 , C12Q2334/00 , C12Q2334/20
摘要： The enzyme substrate according to this invention has within its molecule both a group to be cleaved by an enzyme reaction and a group that forms a strongly fluorescent coumarin derivative through intramolecular lactonization when cleaved by the enzyme reaction. Furthermore, the method for determining an enzyme activity according to this invention comprises conducting the enzyme reaction by the use of the enzyme substrate of the invention and determining the enzyme activity by means of the measurement of fluorescence of the coumarin derivative formed.
摘要翻译：根据本发明的酶底物在其分子内具有通过酶反应切割的基团和当通过酶反应切割时通过分子内内酯形成强荧光香豆素衍生物的基团。此外，根据本发明的确定酶活性的方法包括通过使用本发明的酶底物进行酶反应，并通过测量形成的香豆素衍生物的荧光来测定酶活性。

5. 发明公开

EP1008658A1 SOLID PHASE FOR DETECTING NUCLEIC ACID AND METHOD FOR DETECTING NUCLEIC ACID 审中-公开
标题翻译： FESTPHASE ZUR ERKENNUNG VONNUKLEINSÄURENUND VERFAHREN ZU DEREN ERKENNUNG
公开(公告)号：EP1008658A1
公开(公告)日：2000-06-14
申请号：EP99901147.1
申请日：1999-01-22
申请人： Laboratory of Molecular Biophotonics
发明人： ABE, Satoshi, Lab. of Molecular Biophotonics , TOYONAGA, Shuji, Lab. of Molecular Biophotonics
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6837 , C12Q2565/107 , C12Q2561/119
摘要： A solid phase for detecting a target nucleic acid and a method for detecting a target nucleic acid with the use of the same. The fundamental structure of the above solid phase comprises a fluorochrome bonded, via a linker, to one end of an oligonucleotide containing a base sequence complementary to the target nucleic acid and a probe having a solid-phase binding site bonded, via the solid-phase bond, to the other end. The above detection method comprises measuring the degree of polarization of the fluorescence generated from the probe and thus detecting the target nucleic acid.
摘要翻译：用于检测靶核酸的固相和使用该核酸的靶核酸的检测方法。上述固相的基本结构包括荧光染料，通过接头连接到含有与靶核酸互补的碱基序列的寡核苷酸的一端和经固相结合的固相结合位点的探针债券，到另一端。上述检测方法包括测量由探针产生的荧光的偏振度，从而检测靶核酸。

6. 发明公开

EP0965635A1 METHOD FOR MONITORING TRANSCRIPTIONAL SYNTHESIS OF RNA AND APPARATUS THEREFOR 失效
标题翻译：方法ZURTRANSKRIPTIONSÜBERWACHUNGUNDDAFÜRGEEIGNETE APPARATUR
公开(公告)号：EP0965635A1
公开(公告)日：1999-12-22
申请号：EP98901107.7
申请日：1998-02-03
申请人： Laboratory of Molecular Biophotonics
发明人： IIDA, Yukari, Laboratory of Molecular Biophotonics , KOSHIMOTO, Hiroyuki , KONDO, Satoshi , TSUJI, Akihiko, Laboratory of Mol. Biophotonics
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6818 , C12Q2521/119
摘要： A method for monitoring the transcriptional synthesis of an RNA by measuring the fluorescence of a probe consisting of a pair of oligonucleotide probes, i.e., a donor probe labeled with an energy donor fluorescent molecule and an acceptor probe labeled with an energy acceptor fluorescent molecule, and having a base sequence hybridizable with a part of the base sequence of the transcriptionally synthesized RNA to thereby confirm the initiation and completion of the transcriptional synthesis of the RNA and the synthesis of the full-length RNA.
摘要翻译：通过测量由一对寡核苷酸探针（即由能量供体荧光分子标记的供体探针和由能量受体荧光分子标记的受体探针）组成的探针的荧光测量RNA的转录合成的方法，以及具有与转录合成的RNA的碱基序列的一部分杂交的碱基序列，从而确认RNA的转录合成的开始和完成以及全长RNA的合成。

7. 发明公开

EP0744614A2 Isoelectric point markers for isoelectric focusing with fluorescence detection 失效
标题翻译： Markierer des isoelektrischen Punktes zur Anwendung bei der isoelektrischen Fokussierung mit Fluoreszenzdetektierung
公开(公告)号：EP0744614A2
公开(公告)日：1996-11-27
申请号：EP96105113.3
申请日：1996-03-29
申请人： Laboratory of Molecular Biophotonics
发明人： Shimura, Kiyohito , Kasai, Kenichi , Matsumoto, Hiroyuki , Takamoto, Hisayoshi
IPC分类号： G01N21/00 , G01N27/447
CPC分类号： C07K1/28 , G01N21/6428 , G01N27/44726 , G01N27/44795 , G01N2410/02 , Y10S930/28
摘要： Provided are isoelectric point(pI) markers for isoelectric focusing with fluorescence detection. The markers are fluorescence-labeled oligopeptides which comprise a fluorescence dye bonded chemically to the amino group of N-terminal amino acid of oligopeptide. The marker shows its unique and narrow pI band(peak) in electrophoresis or isoelectric focusing. The markers can be designed to have appropriate pI value, and cover wide range of pI (3
摘要翻译：提供了用于具有荧光检测的等电聚焦的等电点（pI）标记。标记是荧光标记的寡肽，其包含与寡肽的N-末端氨基酸的氨基化学键合的荧光染料。标记显示其在电泳或等电聚焦中的独特且窄的pI带（峰）。标记可以被设计成具有适当的pI值，并且覆盖pI（3

8. 发明公开

EP0884584A1 METHOD AND APPARATUS FOR ASSAYING ENZYMATIC REACTION 失效
标题翻译： VORRICHTUNG UND VERFAHREN ZUM TESTEN ENZYMATISCHER REAKENEN
公开(公告)号：EP0884584A1
公开(公告)日：1998-12-16
申请号：EP97904619.0
申请日：1997-02-24
申请人： Laboratory of Molecular Biophotonics
发明人： OKAZAKI, Shigetoshi , MATSUMOTO, Hiroyuki
IPC分类号： G01N21/75 , G01N21/78 , G01N21/27
CPC分类号： G01N21/552
摘要： A method and apparatus for measuring enzyme activity by supplying a substrate solution and an enzyme solution into a reaction vessel combined with a total reflection absorption prism. A measurement solution (40) in which the enzyme solution and the substrate solution are combined together is kept at a constant temperature within the reaction vessel (26) by a temperature control unit (23), and is stirred by a stirrer (21). Infrared light (36) emitted from an infrared light source (3) is made incident on the interface between a total reflection absorption prism (28) disposed in contact with the measurement solution (40) and the measurement solution (40) from the side of the total reflection absorption prism (28) and is totally reflected by the interface. The spectrum of transmitted infrared light (37) thus totally reflected and emitted is detected by an infrared light detector (5). According to thus detected spectrum, a change in the infrared absorption spectrum or absorbance is determined, whereby the enzyme reaction in the measurement solution (40) is measured.
摘要翻译：一种通过将底物溶液和酶溶液供给到与全反射吸收棱镜组合的反应容器中来测量酶活性的方法和装置。将酶溶液和底物溶液组合在一起的测量溶液（40）通过温度控制单元（23）在反应容器（26）内保持恒定温度，并通过搅拌器（21）搅拌。从红外光源（3）发射的红外光（36）入射到与测量溶液（40）接触设置的全反射吸收棱镜（28）和测量溶液（40）之间的界面上，全反射吸收棱镜（28），并被界面全反射。由红外光检测器（5）检测由此全反射和发射的透射红外光（37）的光谱。根据如此检测的光谱，测定红外吸收光谱或吸光度的变化，由此测量测量溶液（40）中的酶反应。

9. 发明公开

EP1054250A1 PIPETTE ADAPTOR, PIPETTE FOR ABSORBANCE MEASUREMENT, TIP, AND METHOD AND APPARATUS FOR ABSORBANCE MEASUREMENT 有权
标题翻译： ADAPTER用于移液器，移液器吸收测量TIP和方法，吸收测量的装置
公开(公告)号：EP1054250A1
公开(公告)日：2000-11-22
申请号：EP00900831.9
申请日：2000-01-20
申请人： Laboratory of Molecular Biophotonics
发明人： TAGUCHI, Takeshi Lab. of Molecular Biophotonics , HIRAMATSU, Mitsuo Lab. of Molecular Biophotonics
IPC分类号： G01N21/03 , G01N21/27 , B01L3/02
CPC分类号： G01N21/03 , B01L3/0275 , G01N21/0303 , G01N21/27 , G01N2035/0434
摘要： An absorbance measuring pipette (1) comprises a pipette (10) and a pipette adapter (20). The pipette adapter (20) has a pipette attachment portion (21) for receiving the front end of the pipette (10) and a tip attachment portion (22) for attaching a tip (30) thereto, is attachable between the pipette (10) and the tip (30), and has an inner space (20A) continuing to respective internal spaces of the pipette (10) and tip (30) upon attachment thereof. Also, the pipette adapter (20) comprises a test light introducing window (23) for introducing test light into the inner space (20A) from outside, and a reflecting mirror (24) for reflecting toward the sample suction port (31) of the tip (30) by way of an opening of the tip attachment portion (22) the test light introduced into the inner space (20A) through the test light introducing window (23).
摘要翻译：移液管测量吸光度（1）包括吸液管（10）和移液管适配器（20）。移液管适配器（20）具有用于接收所述移液管（10）和尖端附接部分（22）的前端，可连接一个尖端（30）在其上的吸液管安装部（21），是移液管之间附连（10）和尖端（30），并具有继续其在附接respectivement吸管（10）和尖端（30）的内部空间的内部空间（20A）。所以，移液管适配器（20）包括用于将测试光进入内部空间（20A）从外部测试光导入窗（23）和反射镜（24），用于朝向的样品吸入口（31）反射尖端（30），由尖端附接部分（22）通过所述测试光导入窗（23）引入所述内部空间（20A）的测试光的开口部的方式。

10. 发明公开

EP1052293A1 Nucleic acid detection in cytoplasm 有权
标题翻译： Nachweis vonNukleinsäurenim Zytoplasma
公开(公告)号：EP1052293A1
公开(公告)日：2000-11-15
申请号：EP99126030.8
申请日：1999-12-27
申请人： Laboratory of Molecular Biophotonics
发明人： Tsuji, Akihiko, c/o Lab. of Molecular Biophotonics , Hirano, Masahiko, Lab. of Molecular Biophotonics , Koshimoto, Hiroyuki , Ishibashi, Kaname, Lab. of Molecular Biophotonics
IPC分类号： C12Q1/68 , C12Q1/02 , G01N33/50
CPC分类号： C12Q1/68 , C12Q1/6818
摘要： Detection probes labeled with fluorescent dyes, to which are bound nuclear membrane unpermeable molecules via linkers, having base sequences that can hybridize to a target nucleic acid. The probes are introduced into the cytoplasm of a living cell in which the target nucleic acid is present, and the target nucleic acid is detected by measurement of the change in fluorescence of the fluorescent dyes due to the formation of a hybrid of the target nucleic acid and the probes.
摘要翻译：用荧光染料标记的检测探针，其通过接头结合核膜不可渗透分子，具有可与靶核酸杂交的碱基序列。将探针引入到其中存在目标核酸的活细胞的细胞质中，并且通过测量荧光染料的荧光变化由于靶核酸的杂交形成而检测靶核酸和探针。

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