专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP3290432A1 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR POLYPEPTIDE MIXTURES 有权
标题翻译：高效液相色谱法测定多肽混合物
公开(公告)号：EP3290432A1
公开(公告)日：2018-03-07
申请号：EP15890235.3
申请日：2015-04-28
申请人： Hybio Pharmaceutical Co., Ltd.
发明人： TANG, Yangming , HE, Gang , LI, Guotao , TAO, Anjin
IPC分类号： C07K2/00 , C07K14/00 , A61K31/785 , A61K38/16 , A61P25/28
CPC分类号： G01N30/34 , A61K38/03 , B01D15/325 , B01D15/362 , B01D15/363 , C07K1/18 , C07K1/20 , C07K14/00 , G01N21/33 , G01N30/74 , G01N30/8631 , G01N30/88 , G01N33/6803 , G01N2030/8831
摘要： The present invention relates to a high performance liquid chromatography method for polypeptide mixtures. Specifically, the method including the following steps: step (1): preparing a solution of the glatiramer acetate to be tested; step (2): performing gradient elution on a sample to be tested with an anion exchange liquid chromatography, a cation exchange liquid chromatography, or a reversed-phase liquid chromatography; step (3): determining a peak area corresponding to each component of the glatiramer acetate , comparing the peak area with to a peak area of a reference substance to determine whether the content of each component of the sample to be tested is in a qualified range.
摘要翻译：本发明涉及用于多肽混合物的高效液相色谱法。具体的，所述方法包括以下步骤：步骤（1）：制备待测试的醋酸格拉替雷溶液; 步骤（2）：用阴离子交换液相色谱，阳离子交换液相色谱或反相液相色谱对待测样品进行梯度洗脱; 步骤（3）：确定与醋酸格拉替雷的每种组分相对应的峰面积，将峰面积与对照物质的峰面积进行比较，以确定待测样品的每种组分的含量是否在合格范围内。

2. 发明公开

EP3199544A2 PREPARATION METHOD FOR GLATIRAMER ACETATE 有权
标题翻译： GLATIRAMER ACETATE的制备方法
公开(公告)号：EP3199544A2
公开(公告)日：2017-08-02
申请号：EP15844092.5
申请日：2015-11-26
申请人： Hybio Pharmaceutical Co., Ltd
发明人： YUAN, Huixing , LI, Guotao , LIU, Jian , MA, Yaping , YUAN, Jiancheng
IPC分类号： C07K14/00 , C07K1/02 , C07K1/06
CPC分类号： C08G69/04 , C07K2/00 , C08G69/36
摘要： The present invention relates to a method for preparing glatiramer acetate, comprising the following steps: (1) dissolving L-alanine NCA, L-tyrosine NCA, L-glutamic acid-γ-benzyl ester NCA, and L-ε-trifluoroacetyl-lysine NCA in 1,4-dioxane as solvent, and stirring until a clarified solution is formed; (2) adding diethylamine for catalysis, stirring at 20-25°C, then slowly pouring the reaction solution into water, and collecting the produced white product; (3) adding the obtained product to a solution of hydrobromic acid in acetic acid, stirring at 23.0-25.0°C, pouring the reaction solution into purified water for quenching and stirring, subjecting the mixture to suction filtration to obtain a yellow solid, after repeating 3-5 times, subjecting the solid to blast drying to remove the moisture therein; and (4) dissolving the solid obtained in step (3) in a 1M piperidine aqueous solution at room temperature and stirring, subjecting the obtained solution to dialysis, adding glacial acetic acid to adjust the pH to 5.5-7.0, and lyophilizing.
摘要翻译：（1）将L-丙氨酸NCA，L-酪氨酸NCA，L-谷氨酸-γ-苄基酯NCA和L-ε-三氟乙酰基赖氨酸溶解于乙醇中， NCA在1,4-二恶烷中作为溶剂，并搅拌直至形成澄清溶液; （2）加入二乙胺催化，在20-25℃下搅拌，然后将反应溶液缓慢倒入水中，收集生成的白色产物; （3）将得到的产物加入到氢溴酸的乙酸溶液中，在23.0-25.0℃下搅拌，将反应溶液倒入精制水中骤冷并搅拌，将混合物抽滤，得到黄色固体重复3-5次，对固体进行鼓风干燥以除去其中的水分; （4）将步骤（3）中得到的固体在室温下溶解于1M哌啶水溶液中并搅拌，将得到的溶液进行透析，加入冰醋酸调节pH至5.5-7.0，冷冻干燥。

3. 发明公开

EP2813514A1 METHOD FOR PURIFYING SOLID-PHASE SYNTHETIC CRUDE LIRAGLUTIDE 有权
标题翻译： VERFAHREN ZUR REINIGUNG VON SYNTHETISCHEM FESTPHASEN-ROHLIRAGLUTID
公开(公告)号：EP2813514A1
公开(公告)日：2014-12-17
申请号：EP13746833.6
申请日：2013-01-29
申请人： Hybio Pharmaceutical Co., Ltd.
发明人： QIN, Liangzheng , PAN, Junfeng , MA, Yaping , YUAN, Jiancheng
IPC分类号： C07K14/605
CPC分类号： C07K14/605 , B01D15/166 , B01D15/1871 , C07K1/16
摘要： The present invention relates to the field of biomedicine, and in particular, to a method for purifying solid-phase synthetic crude liraglutide. The method comprises: dissolving solid-phase synthetic crude liraglutide in an aqueous acetonitrile solution to obtain a crude peptide solution; and obtaining liraglutide with high purity and high yield through four-step HPLC purification.
摘要翻译：本发明涉及生物医学领域，特别涉及一种纯化固相合成粗利拉鲁肽的方法。该方法包括：将固相合成粗利拉鲁肽溶于乙腈水溶液中，得到粗肽溶液; 并通过四步HPLC纯化获得高纯度和高产率的利拉鲁肽。

4. 发明授权

EP3398959B1 METHOD FOR PREPARING LIXISENATIDE 有权
公开(公告)号：EP3398959B1
公开(公告)日：2020-11-25
申请号：EP16880982.0
申请日：2016-12-16
申请人： Hybio Pharmaceutical Co., Ltd.
发明人： CHEN, Xinliang , MI, Pengcheng , TAO, Anjin , YUAN, Jiancheng
IPC分类号： C07K14/605 , C07K1/06

5. 发明公开

EP3199544A4 PREPARATION METHOD FOR GLATIRAMER ACETATE 有权
公开(公告)号：EP3199544A4
公开(公告)日：2018-06-27
申请号：EP15844092
申请日：2015-11-26
申请人： HYBIO PHARMACEUTICAL CO LTD
发明人： YUAN HUIXING , LI GUOTAO , LIU JIAN , MA YAPING , YUAN JIANCHENG
IPC分类号： C07K14/00 , C07K1/02 , C07K1/06 , C07K2/00
CPC分类号： C08G69/04 , C07K2/00 , C08G69/36
摘要： The present invention relates to a method for preparing glatiramer acetate, comprising the following steps: (1) dissolving L-alanine NCA, L-tyrosine NCA, L-glutamic acid-³-benzyl ester NCA, and L-µ-trifluoroacetyl-lysine NCA in 1,4-dioxane as solvent, and stirring until a clarified solution is formed; (2) adding diethylamine for catalysis, stirring at 20-25°C, then slowly pouring the reaction solution into water, and collecting the produced white product; (3) adding the obtained product to a solution of hydrobromic acid in acetic acid, stirring at 23.0-25.0°C, pouring the reaction solution into purified water for quenching and stirring, subjecting the mixture to suction filtration to obtain a yellow solid, after repeating 3-5 times, subjecting the solid to blast drying to remove the moisture therein; and (4) dissolving the solid obtained in step (3) in a 1M piperidine aqueous solution at room temperature and stirring, subjecting the obtained solution to dialysis, adding glacial acetic acid to adjust the pH to 5.5-7.0, and lyophilizing.

6. 发明授权

EP2537856B1 METHOD FOR PREPARING ATOSIBAN ACETATE 有权
标题翻译：制备阿托西班醋酸酯的方法
公开(公告)号：EP2537856B1
公开(公告)日：2017-09-13
申请号：EP11850254.1
申请日：2011-12-22
申请人： Hybio Pharmaceutical Co., Ltd
发明人： XIAO, Qing , LIU, Jian , LI, Hongling , MA, Yaping , YUAN, Jiancheng
IPC分类号： C07K7/06 , A61K38/08 , A61P15/06
CPC分类号： C07K7/64 , C07K7/16

7. 发明公开

EP2537856A4 METHOD FOR PREPARING ATOSIBAN ACETATE 有权
标题翻译： VERFAHREN ZUR HERSTELLUNG VON ATOSIBAN-ACETAT
公开(公告)号：EP2537856A4
公开(公告)日：2013-12-04
申请号：EP11850254
申请日：2011-12-22
申请人： HYBIO PHARMACEUTICAL CO LTD
发明人： XIAO QING , LIU JIAN , LI HONGLING , MA YAPING , YUAN JIANCHENG
IPC分类号： C07K7/06 , A61K38/08 , A61P15/06
CPC分类号： C07K7/64 , C07K7/16
摘要： The present invention provides a method for preparing atosiban acetate. The method comprises the following steps of: synthesizing to obtain linear atosiban; dissolving the linear atosiban in an acetonitrile aqueous solution, adjusting the pH value with ammonia water, adding H 2 O 2 for oxidizing, filtering, purifying, and transferring salt to obtain the atosiban acetate. In the present invention, an appropriate route is provided, the linear atosiban is synthesized by adopting a solid phase method, and the atosiban is obtained by liquid phase oxidation. The method has the advantages of capabilities of solving the problem of insolubility of the linear atosiban, reducing the reaction size to the maximum extent and shortening reaction time, and being high in yield and easy to industrialize.
摘要翻译：本发明提供了一种制备醋酸托西班醋酸酯的方法。该方法包括以下步骤：合成得到线性阿托西班; 将线性阿托西班溶解在乙腈水溶液中，用氨水调节pH值，加入H 2 O 2进行氧化，过滤，纯化和转移盐，得到醋酸阿托品。在本发明中，提供了适当的路线，通过采用固相法合成线性阿托西班，通过液相氧化获得阿托西班。该方法具有解决线性阿托西班不溶性问题的能力，最大限度地减少反应尺寸，缩短反应时间，产率高，易于工业化的优点。

8. 发明公开

EP2537856A1 METHOD FOR PREPARING ATOSIBAN ACETATE 有权
标题翻译：用于生产醋酸阿托西班
公开(公告)号：EP2537856A1
公开(公告)日：2012-12-26
申请号：EP11850254.1
申请日：2011-12-22
申请人： Hybio Pharmaceutical Co., Ltd
发明人： XIAO, Qing , LIU, Jian , LI, Hongling , MA, Yaping , YUAN, Jiancheng
IPC分类号： C07K7/06 , A61K38/08 , A61P15/06
CPC分类号： C07K7/64 , C07K7/16
摘要： The present invention provides a method for preparing atosiban acetate. The method comprises the following steps of: synthesizing to obtain linear atosiban; dissolving the linear atosiban in an acetonitrile aqueous solution, adjusting the pH value with ammonia water, adding H 2 O 2 for oxidizing, filtering, purifying, and transferring salt to obtain the atosiban acetate. In the present invention, an appropriate route is provided, the linear atosiban is synthesized by adopting a solid phase method, and the atosiban is obtained by liquid phase oxidation. The method has the advantages of capabilities of solving the problem of insolubility of the linear atosiban, reducing the reaction size to the maximum extent and shortening reaction time, and being high in yield and easy to industrialize.

9. 发明公开

EP3805246A1 METHOD FOR PURIFYING LONG CHAIN POLYPEPTIDE 审中-实审
公开(公告)号：EP3805246A1
公开(公告)日：2021-04-14
申请号：EP18921212.9
申请日：2018-05-30
申请人： Hybio Pharmaceutical Co., Ltd.
发明人： YIN, Chuanlong , MI, Pengcheng , TAO, Anjin , YUAN, Jiancheng
IPC分类号： C07K1/16 , C07K14/58
摘要： A method for purifying a long chain polypeptide includes: 1) purification step: connecting two chromatographic columns in series to separate a crude product, in which the particle size of a packing in an upstream chromatographic column is larger than that in a downstream chromatographic column; optionally, the method further includes step 2): using the upstream chromatographic column in step 1) for a salt conversion, loading the target peak product obtained in step 1) and rinsing with 95-85% of the A2 and 5-15% of the B for 15-30 min for a desalination, wherein A2 phase is an acetic acid aqueous solution with a volume ratio of 0.05%-0.2%; B phase is an organic phase acetonitrile, and the detection wavelength is 230 nm.

10. 发明公开

EP3392266A1 LINACLOTIDE SYNTHESIS METHOD 有权转让
公开(公告)号：EP3392266A1
公开(公告)日：2018-10-24
申请号：EP16874867.1
申请日：2016-12-15
申请人： Hybio Pharmaceutical Co., Ltd
发明人： CHEN, Xueming , WU, Jingkang , MI, Pengcheng , TAO, Anjin , YUAN, Jiancheng
IPC分类号： C07K7/08 , C07K1/20 , C07K1/06 , C07K1/04
摘要： Disclosed is a method of synthesizing linaclotide through completely selective formation of three disulfide bonds, comprising the steps of: 1) synthesizing linaclotide precursor resin through solid-phase synthesis; 2) forming the first disulfide bond through solid phase oxidation; 3) forming the second disulfide bond through liquid phase oxidation; and 4) deprotecting methyl in the methyl-protected cysteine, and oxidatively coupling the third disulfide bond to obtain linaclotide. The method has mild reaction conditions with low cost, high yield and high purity product, is a simple and stable process and is suitable for large-scale production.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式