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1. 发明授权

EP3356554B1 METHODS FOR SUBTYPING DIFFUSE B-CELL LYMPHOMA (DLBCL) 有权
公开(公告)号：EP3356554B1
公开(公告)日：2019-10-30
申请号：EP16782152.9
申请日：2016-09-29
申请人： HTG Molecular Diagnostics, Inc.
发明人： LAFLEUR, Bonnie , LIU, Qian , LUECKE, John, W. , ROCHE, Patrick, C.
IPC分类号： C12Q1/6886

2. 发明公开

EP3066218A1 METHODS FOR DETECTING NUCLEIC ACIDS 有权
标题翻译： NACHWEISVERFAHRENFÜRNUKLEINSÄUREN
公开(公告)号：EP3066218A1
公开(公告)日：2016-09-14
申请号：EP14859794.1
申请日：2014-11-05
申请人： HTG Molecular Diagnostics, Inc.
发明人： ROUNSEVILLE, Matt , MODUR, Vijay
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q2521/327 , C12Q2521/501 , C12Q2521/514 , C12Q2525/161 , C12Q2533/107 , C12Q2537/125 , C12Q2561/125 , C12Q2565/519 , C12Q2521/319
摘要： Disclosed herein are methods for detecting a target nucleic acid molecule in a sample. The methods can include contacting a sample with a detectably labeled probe (detection probe) that specifically binds to a first target sequence in the target nucleic acid molecule, a bifunctional oligonucleotide including a portion that specifically binds to a second target sequence in the target nucleic acid molecule and a portion that specifically binds to an anchor, and a surface comprising the anchor. Specifically bound detection probe and bifunctional oligonucleotide are ligated and a reagent that specifically removes substantially all of the target nucleic acid is added. Unligated detection probe is removed and presence of the detectable label is detected. In other embodiments, the ligation and/or removal of target nucleic acid are omitted and the detection probe specifically bound to the target nucleic acid is detected.
摘要翻译：本文公开了检测样品中靶核酸分子的方法。所述方法可以包括将样品与特异性结合靶核酸分子中的第一靶序列的可检测标记的探针（检测探针）接触，双功能寡核苷酸，包括特异性结合靶核酸中的第二靶序列的部分分子和特异性结合锚的部分，以及包含锚的表面。连接特异性结合的检测探针和双功能寡核苷酸，并添加特异性去除基本上所有靶核酸的试剂。去除未螯合的检测探针并检测可检测标记的存在。在其它实施方案中，省略了靶核酸的连接和/或去除，并检测到与靶核酸特异性结合的检测探针。

3. 发明授权

EP2726628B1 METHODS OF DETECTING GENE FUSIONS 有权
标题翻译：检测方法的基因融合体
公开(公告)号：EP2726628B1
公开(公告)日：2016-05-11
申请号：EP11868918.1
申请日：2011-12-07
申请人： HTG Molecular Diagnostics, Inc.
发明人： SELIGMANN, Bruce , KERNS, BJ , LUECKE, John , ROUNSEVILLE, Matt , BOTROS, Ihab , SCHWARTZ, Mark
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q1/6827 , C12Q1/6886 , C12Q2600/156 , C12Q2521/307 , C12Q2537/16 , C12Q2565/102

4. 发明授权

EP3066218B1 METHODS FOR DETECTING NUCLEIC ACIDS 有权
公开(公告)号：EP3066218B1
公开(公告)日：2018-12-26
申请号：EP14859794.1
申请日：2014-11-05
申请人： HTG Molecular Diagnostics, Inc.
发明人： ROUNSEVILLE, Matt , MODUR, Vijay
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q2521/327 , C12Q2521/501 , C12Q2521/514 , C12Q2525/161 , C12Q2533/107 , C12Q2537/125 , C12Q2561/125 , C12Q2565/519 , C12Q2521/319
摘要： Disclosed herein are methods for detecting a target nucleic acid molecule in a sample. The methods can include contacting a sample with a detectably labeled probe (detection probe) that specifically binds to a first target sequence in the target nucleic acid molecule, a bifunctional oligonucleotide including a portion that specifically binds to a second target sequence in the target nucleic acid molecule and a portion that specifically binds to an anchor, and a surface comprising the anchor. Specifically bound detection probe and bifunctional oligonucleotide are ligated and a reagent that specifically removes substantially all of the target nucleic acid is added. Unligated detection probe is removed and presence of the detectable label is detected. In other embodiments, the ligation and/or removal of target nucleic acid are omitted and the detection probe specifically bound to the target nucleic acid is detected.

5. 发明公开

EP2867373A4 NUCLEASE PROTECTION METHODS FOR DETECTION OF NUCLEOTIDE VARIANTS 有权
标题翻译：核酸用于检测核苷酸变异
公开(公告)号：EP2867373A4
公开(公告)日：2016-05-25
申请号：EP13809718
申请日：2013-06-28
申请人： HTG MOLECULAR DIAGNOSTICS INC
发明人： ROUNSEVILLE MATT , SELIGMANN BRUCE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6827 , C12Q2521/325 , C12Q2561/108 , C12Q2537/143 , C12Q2563/107
摘要： Disclosed herein are methods for detecting presence of a nucleotide variant in a target nucleic acid utilizing a nuclease protection assay. The methods include contacting a sample with at least two probes, wherein the first probe is complementary to the wild-type (non-variant) nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid and the second probe is complementary to the variant nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid, under conditions sufficient for the probes to hybridize to the target nucleic acid, producing a mixture of hybridized and unhybridized nucleic acids. The mixture is contacted with a nuclease specific for single-stranded nucleic acid molecules under conditions sufficient to remove unhybridized nucleic acid molecules (or unhybridized portions of nucleic acid molecules). The presence of the at least two probes is then detected, thereby detecting the presence of the variant and/or non-variant target nucleic acid in the sample.

6. 发明公开

EP2496715A1 QUANTITATIVE NUCLEASE PROTECTION SEQUENCING (QNPS) 有权
标题翻译：定量核酸测序（QNPS）
公开(公告)号：EP2496715A1
公开(公告)日：2012-09-12
申请号：EP10781757.9
申请日：2010-11-03
申请人： HTG Molecular Diagnostics, Inc.
发明人： SELIGMANN, Bruce
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q2537/163 , C12Q2521/325
摘要： The present invention provides a new approach, quantitative Nuclease Protection Sequencing (qNPS™), for addressing several challenges that face sequencing and which provides improvements for research and diagnostic applications. The method uses a lysis-only nuclease protection assay to generate nucleic acid, e.g., DNA probes for sequencing, which can be coupled to gene-specific tags to permit the identification of the gene without necessitating the sequencing of the nuclease protection probe itself and/or can be coupled to experiment-specific tags whereby samples from different patients can be combined into a single run. The disclosed qNPS makes sequencing fixed or insoluble samples possible and affordable as a research and discovery tool and as a diagnostic test.

7. 发明公开

EP3906321A1 METHODS OF DETECTING DNA AND RNA IN THE SAME SAMPLE 审中-实审
公开(公告)号：EP3906321A1
公开(公告)日：2021-11-10
申请号：EP19828939.9
申请日：2019-12-02
申请人： HTG Molecular Diagnostics, Inc.
发明人： THOMPSON, Debrah , ROUNSEVILLE, Matthew
IPC分类号： C12Q1/6853

8. 发明公开

EP3356554A1 METHODS FOR SUBTYPING DIFFUSE B-CELL LYMPHOMA (DLBCL) 有权
公开(公告)号：EP3356554A1
公开(公告)日：2018-08-08
申请号：EP16782152.9
申请日：2016-09-29
申请人： HTG Molecular Diagnostics, Inc.
发明人： LAFLEUR, Bonnie , LIU, Qian , LUECKE, John, W. , ROCHE, Patrick, C.
IPC分类号： C12Q1/68
摘要： Described herein are innovations for classifying subtypes of DLBCL, as well as using the results of classification for diagnosis, prognosis, and therapy selection. In this way, the classifier can effectively classify subtypes of DLBCL and provide meaningful output for the benefit of medical practices and DLBCL patients. Also described are arrays and kits that can be used to measure expression of DLBCL signatures genes.

9. 发明公开

EP2971284A1 SUBTYPING LUNG CANCERS 审中-公开
标题翻译：打字肺癌组织
公开(公告)号：EP2971284A1
公开(公告)日：2016-01-20
申请号：EP14768337.9
申请日：2014-03-03
申请人： HTG Molecular Diagnostics, Inc.
发明人： ROBERTS, Chris , WANG, Hui , LU, Zhenquiang , MADDULA, Krishna , RUA, Sam , KNAPP, Kevin , LAWSON, Byron , THOMPSON, Debrah , HRUBIAK, Michael , BREEDLOVE, Tyler , MODUR, Vijay
IPC分类号： C40B30/04
CPC分类号： G06F19/20 , C12Q1/6886 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16 , G01N33/57423
摘要： This disclosure concerns the identification of biomarkers that are characteristic of squamous or non-squamous (e.g., adenocarcinoma, large cell carcinoma, carcinoid tumor, sarcomatoid carcinoma) subtypes of non-small cell lung cancer (NSCLC), clinically useful NSCLC classifiers, kits and arrays for distinguishing squamous and nonsquamous NSCLC subtypes, bioinformatic methods for determining clinically useful classifiers, and methods of use of each of the foregoing.

10. 发明公开

EP2867373A1 NUCLEASE PROTECTION METHODS FOR DETECTION OF NUCLEOTIDE VARIANTS 有权
标题翻译： NUKLEASESCHUTZVERFAHREN ZUR DETEKTION VON NUKLEOTIDVARIANTEN
公开(公告)号：EP2867373A1
公开(公告)日：2015-05-06
申请号：EP13809718.3
申请日：2013-06-28
申请人： HTG Molecular Diagnostics, Inc.
发明人： ROUNSEVILLE, Matt , SELIGMANN, Bruce
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6827 , C12Q2521/325 , C12Q2561/108 , C12Q2537/143 , C12Q2563/107
摘要： Disclosed herein are methods for detecting presence of a nucleotide variant in a target nucleic acid utilizing a nuclease protection assay. The methods include contacting a sample with at least two probes, wherein the first probe is complementary to the wild-type (non- variant) nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid and the second probe is complementary to the variant nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid, under conditions sufficient for the probes to hybridize to the target nucleic acid, producing a mixture of hybridized and unhybridized nucleic acids. The mixture is contacted with a nuclease specific for single-stranded nucleic acid molecules under conditions sufficient to remove unhybridized nucleic acid molecules (or unhybridized portions of nucleic acid molecules). The presence of the at least two probes is then detected, thereby detecting the presence of the variant and/or non- variant target nucleic acid in the sample.
摘要翻译：本文公开了使用核酸酶保护测定法检测靶核酸中核苷酸变体的存在的方法。所述方法包括使样品与至少两个探针接触，其中第一探针与靶核酸中的核苷酸变体位置处的野生型（非变体）核苷酸互补，第二探针是与目标核酸的核苷酸变体位置的变体核苷酸互补，在足以使探针与靶核酸杂交的条件下，产生杂交和未杂交的核酸的混合物。在足以除去未杂交的核酸分子（或核酸分子的未杂交部分）的条件下，使混合物与单链核酸分子特异性的核酸酶接触。然后检测至少两个探针的存在，从而检测样品中变体和/或非变体靶核酸的存在。

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