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1. 发明授权

EP2787565B1 Transposon end compositions and methods for modifying nucleic acids 有权转让
标题翻译： Transposonendenzusammensetzungen和用于核酸的修饰方法
公开(公告)号：EP2787565B1
公开(公告)日：2015-09-16
申请号：EP14171077.2
申请日：2009-10-24
申请人： Epicentre Technologies Corporation
发明人： Jendrisak, Jerome , Dahl, Gary , Grunenwald, Haiying Li , Caruccio, Nicholas
IPC分类号： H01M6/14 , H01M6/50 , H01M10/42
CPC分类号： C12N9/22 , C12N9/1252 , C12N9/93 , C12N15/10 , C12N15/1065 , C12N15/1093 , C12N15/66 , C12P19/34 , C12Q1/6806 , C12Q2525/155 , C12Q2535/122 , C12Q2565/518

2. 发明公开

EP2610352A1 Template-independent ligation of single-stranded DNA 有权转让
标题翻译： Vorlagenunabhängige连接von Einzelstrang-DNA
公开(公告)号：EP2610352A1
公开(公告)日：2013-07-03
申请号：EP13155136.8
申请日：2010-02-16
申请人： Epicentre Technologies Corporation
发明人： Jendrisak, Jerome , Decker, Joanne , Dahl, Gary
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12N9/93 , C12N15/10 , C12N15/66 , C12Q1/68 , C12Q1/6869 , C12Q2521/501 , C12Q2525/307 , C12Q2527/137
摘要： The invention provides ligation reaction mixtures, methods, and kits for improved template-independent intramolecular ligation (circularization) of linear ssDNA, including denatured gDNA fragments or first-strand cDNA made by reverse transcription of RNA, using, for example, a thermostable RNA ligase. The circular ssDNA molecules obtained using the improved ligation reaction mixtures and methods can be used, for example, as templates: for amplification by inverse PCR, rolling circle replication, transcription, or for massively parallel DNA sequencing. Applications include, for example: gene expression analysis by qPCR or using microarrays; analysis of gDNA copy number variation; and detection or quantification of specific nucleic acid sequences for research, screening, medical diagnostics, theranostics, personalized medical treatment or breeding, for purposes such as human or animal medicine, forensics, or agriculture.
摘要翻译：本发明提供了用于改进线性ssDNA的模板无关分子内连接（环化）的连接反应混合物，方法和试剂盒，其包括使用例如热稳定的RNA连接酶，通过逆转录RNA制备的变性gDNA片段或第一链cDNA 。使用改进的连接反应混合物和方法获得的环状ssDNA分子可用于例如模板：用于通过反向PCR扩增，滚环复制，转录或大规模并行DNA测序。应用包括例如：通过qPCR进行基因表达分析或使用微阵列; 分析gDNA拷贝数变异; 检测或定量用于研究，筛选，医学诊断，治疗，个性化医疗或育种的特定核酸序列，用于人或动物医学，法医学或农业学等。

3. 发明公开

EP2508529A1 Transposon end compositions and methods for modifying nucleic acids 有权转让
标题翻译： Transposonendenzusammensetzungen和用于核酸的修饰方法
公开(公告)号：EP2508529A1
公开(公告)日：2012-10-10
申请号：EP12175461.8
申请日：2009-10-24
申请人： Epicentre Technologies Corporation
发明人： Jendrisak, Jerome , Dahl, Gary , Grunenwald, Haiying Li , Caruccio, Nicholas
IPC分类号： C07H21/00 , C12N9/00 , C12P19/34
CPC分类号： C12N9/22 , C12N9/1252 , C12N9/93 , C12N15/10 , C12N15/1065 , C12N15/1093 , C12N15/66 , C12P19/34 , C12Q1/6806 , C12Q2525/155 , C12Q2535/122 , C12Q2565/518
摘要： The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5'-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5'- and 3'-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5'-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3'-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5'- and 3'-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called "next-generation sequencing.)

4. 发明公开

EP2376517A1 TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS 有权转让
标题翻译： TRANSPOSONENDENZUSAMMENSETZUNGEN和方法进行修改的NUCLEIC
公开(公告)号：EP2376517A1
公开(公告)日：2011-10-19
申请号：EP09822831.5
申请日：2009-10-24
申请人： Epicentre Technologies Corporation
发明人： JENDRISAK, Jerome , DAHL, Gary , GRUNENWALD, Haiying Li , CARUCCIO, Nicholas
IPC分类号： C07H21/00 , C12N9/00 , C12P19/34
CPC分类号： C12N9/22 , C12N9/1252 , C12N9/93 , C12N15/10 , C12N15/1065 , C12N15/1093 , C12N15/66 , C12P19/34 , C12Q1/6806 , C12Q2525/155 , C12Q2535/122 , C12Q2565/518
摘要： The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5'-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5'- and 3'-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5'-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3'-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5'- and 3'-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called "next-generation sequencing.)

5. 发明公开

EP1576188A2 METHODS FOR USING RIBOPRIMERS FOR STRAND DISPLACEMENT REPLICATION OF TARGET SEQUENCES 有权
标题翻译：方法使用靶序列RIBOPRIMERN链置换复制的
公开(公告)号：EP1576188A2
公开(公告)日：2005-09-21
申请号：EP03789924.2
申请日：2003-11-21
申请人： Epicentre Technologies Corporation
发明人： DAHL, Gary, A. , JENDRISAK, Jerome, J. , RADEK, Agnes, J.
IPC分类号： C12Q1/68 , C12P19/34 , C07H19/00 , C07H21/04
CPC分类号： C12N15/1096 , C12Q1/6853 , C12Q2531/119 , C12Q2521/301 , C12Q2525/125
摘要： Methods, compositions and kits kits for amplifying a target sequence by strand displacement replication using strand-displacing primers. The method uses primers that have only ribonucleotides or purine ribonucleotides and at least one 2’-substituted pyrimidine-2’-deoxyribonucleotide.

6. 发明授权

EP3272879B1 TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS 有权转让
公开(公告)号：EP3272879B1
公开(公告)日：2019-08-07
申请号：EP17171687.1
申请日：2009-10-24
申请人： Epicentre Technologies Corporation
发明人： JENDRISAK, Jerome , DAHL, Gary , GRUNENWALD, Haiying Li , CARUCCIO, Nicholas
IPC分类号： C12Q1/68

7. 发明授权

EP2283132B1 SELECTIVE 5' LIGATION TAGGING OF RNA 有权转让
标题翻译：选择性5个-LIGATIONS标记RNA的
公开(公告)号：EP2283132B1
公开(公告)日：2016-10-26
申请号：EP09740014.7
申请日：2009-05-04
申请人： Epicentre Technologies Corporation
发明人： JENDRISAK, Jerome, J. , VAIDYANATHAN, Ramesh , DAHL, Gary
IPC分类号： C12N15/10 , C12N15/11 , C07H21/02 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1096 , C12Q1/6855 , C12Q2525/207 , C12Q2525/125 , C12Q2521/525
摘要： The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

8. 发明授权

EP2816111B1 Methods, compositions, and kits for generating rRNA-depleted samples or isolating rRNA from samples 有权转让
标题翻译：用于产生rRNA的差样品或为rRNA基因的从样品中分离的方法，组合物和试剂盒
公开(公告)号：EP2816111B1
公开(公告)日：2016-04-13
申请号：EP14181813.8
申请日：2010-08-13
申请人： Epicentre Technologies Corporation
发明人： Sooknanan, Roy R.
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2525/117 , C12Q2563/131
摘要： The present invention provides methods, compositions, and kits for generating rRNA-depleted samples and for isolating rRNA from samples. In particular, the present invention provides compositions comprising affinity-tagged antisense rRNA molecules corresponding to substantially all of at least one rRNA molecule (e.g., 28S, 26S, 25S, 18S, 5.8S and 5S eukaryotic cytoplasmic rRNA molecules, 12S and 16S eukaryotic mitochondrial rRNA molecules, and 23S, 16S and 5S prokaryotic rRNA molecules) and methods for using such compositions to generate rRNA-depleted samples or to isolate rRNA molecules from samples.
摘要翻译：本发明提供了用于产生的rRNA耗尽样品和从样品中分离的rRNA方法，组合物和试剂盒。特别地，本发明提供组合物，包含亲和标记的反义分子的rRNA对应于基本上所有的至少一个的rRNA分子（例如，28S，26S，25S，18S，5.8S和5S真核细胞质的rRNA分子，12S和16S真核线粒体的 rRNA基因分子，和23S，16S和5S rRNA基因分子的原核）的方法和使用组合物的搜索以生成rRNA的耗尽样品或rRNA的分子从样本中分离。

9. 发明公开

EP2816111A1 Methods, compositions, and kits for generating rRNA-depleted samples or isolating rRNA from samples 有权转让
标题翻译：用于产生rRNA的差样品或为rRNA基因的从样品中分离的方法，组合物和试剂盒
公开(公告)号：EP2816111A1
公开(公告)日：2014-12-24
申请号：EP14181813.8
申请日：2010-08-13
申请人： Epicentre Technologies Corporation
发明人： Sooknanan, Roy R.
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2525/117 , C12Q2563/131
摘要： The present invention provides methods, compositions, and kits for generating rRNA-depleted samples and for isolating rRNA from samples. In particular, the present invention provides compositions comprising affinity-tagged antisense rRNA molecules corresponding to substantially all of at least one rRNA molecule (e.g., 28S, 26S, 25S, 18S, 5.8S and 5S eukaryotic cytoplasmic rRNA molecules, 12S and 16S eukaryotic mitochondrial rRNA molecules, and 23S, 16S and 5S prokaryotic rRNA molecules) and methods for using such compositions to generate rRNA-depleted samples or to isolate rRNA molecules from samples.

10. 发明授权

EP2508529B1 Transposon end compositions and methods for modifying nucleic acids 有权转让
标题翻译： Transposonendenzusammensetzungen和用于核酸的修饰方法
公开(公告)号：EP2508529B1
公开(公告)日：2013-08-28
申请号：EP12175461.8
申请日：2009-10-24
申请人： Epicentre Technologies Corporation
发明人： Jendrisak, Jerome , Dahl, Gary , Grunenwald, Haiying Li , Caruccio, Nicholas
IPC分类号： C07H21/00 , C12N9/00 , C12P19/34
CPC分类号： C12N9/22 , C12N9/1252 , C12N9/93 , C12N15/10 , C12N15/1065 , C12N15/1093 , C12N15/66 , C12P19/34 , C12Q1/6806 , C12Q2525/155 , C12Q2535/122 , C12Q2565/518
摘要： The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5'-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5'- and 3'-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5'-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3'-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5'- and 3'-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called "next-generation sequencing.)

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