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    • 6. 发明公开
    • TESTING METHODS AND APPARATUS
    • TESTVERFAHREN UND -VORRICHTUNG
    • EP3117007A1
    • 2017-01-18
    • EP15710557.8
    • 2015-03-09
    • Bactest Limited
    • RICKETT, Toby WilliamPRICE, DerekBROOKING, Annie
    • C12Q1/04C12M1/34
    • C12Q1/04C12M23/34C12M37/00C12M41/12C12M41/36C12M41/40C12M41/46C12Q1/045
    • We describe a method of selectively testing a liquid sample for the presence of a specific living biological entity, the method comprising: preparing a plurality of culture vessels each for selective detection of the presence of a different living biological entity, wherein preparing a said culture vessel comprises: providing a culture vessel comprising a sealable chamber for receiving and culturing a liquid sample such that presence of said biological entity is detectable by detecting a change in pressure in a head space of said chamber;providing a selective growth medium in said culture vessel, wherein said selective growth medium is in a dry form and selectively facilitates growth of a specific living biological entity, and providing the culture vessel with an identifier to identity said specific biological entity;providing a test system comprising a device to receive said culture vessel and to detect said change in pressure in said head space of said chamber, the device further comprising a temperature control system, and a data processor to monitor said detected change in pressure, to control said temperature control system to regulate a temperature of a liquid sample in said culture vessel, and to signal detection of the presence of a biological entity in said culture vessel from said detected change in pressure;storing a plurality of entity- selective test protocols in a memory of said test system,wherein a said test protocol defines one or both of a temperature of incubation of said liquid sample in said culture vessel and a threshold change in pressure in said head space of said chamber for detection of the target entity, wherein said test protocols are respectively identified according to one of a plurality of specific biological entities for which they selectively facilitate growth;selecting,from said plurality of culture vessels, a culture vessel to selectively detect a target said specific biological entity;providing a test liquid sample to said chamber of said selected culture vessel;using the identifier on said elected culture vessel to select a corresponding test protocol for said target specific biological entity; and running said selected test protocol on said test system to incubate said test liquid sample according to said test protocol to determine whether said target specific biological entity is present within said test liquid sample;wherein said determination comprises selection dependent upon both said selective growth medium and said entity-selective test protocol.
    • 我们描述了选择性测试液体样品以存在特定活体生物实体的方法,所述方法包括:制备多个培养容器,每个培养容器用于选择性检测不同活体生物体的存在,其中制备所述培养容器 包括:提供包含用于接收和培养液体样品的可密封室的培养容器,使得通过检测所述室的顶部空间中的压力变化来检测所述生物体的存在; 在所述培养容器中提供选择性生长培养基,其中所述选择性生长培养基是干燥形式并选择性促进特定生物体实体的生长,并为培养容器提供识别所述特定生物实体的标识符; 提供一种包括接收所述培养容器的装置并检测所述室的所述顶部空间中的所述压力变化的测试系统,所述装置还包括温度控制系统和用于监视所述检测到的压力变化的数据处理器,以控制 所述温度控制系统用于调节所述培养容器中的液体样品的温度,并从所述检测到的压力变化来信号检测所述培养容器中生物实体的存在; 将多个实体选择性测试协议存储在所述测试系统的存储器中,其中所述测试协议定义所述培养容器中所述液体样品的温育温度和所述培养容器中的所述顶部空间中的阈值变化 所述检测目标实体室,其中所述测试方案分别根据多个特定生物实体中的一个识别,所述实体有选择地促进生长; 从所述多个培养容器中选择培养容器,以选择性地检测所述特定生物实体的靶; 向所述选择的培养容器的所述室提供测试液体样品; 使用所述选择性培养容器上的标识符选择所述目标特异性生物实体的相应测试方案; 以及在所述测试系统上运行所述选定的测试方案,以根据所述测试方案孵育所述测试液体样品,以确定所述目标特异性生物实体是否存在于所述测试液体样品内; 其中所述确定包括依赖于所述选择性生长培养基和所述实体选择性测试方案的选择。