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    • 6. 发明公开
    • SEQUENCING METHODS
    • SEQUENZIERUNGSVERFAHREN
    • EP3134542A1
    • 2017-03-01
    • EP15719281.6
    • 2015-04-27
    • DNAE GROUP HOLDINGS LIMITED
    • MORLEY, Daniel
    • C12Q1/68G06F19/22
    • Described is a method for sequencing a polynucleotide strand by sequencing by synthesis, the method including selecting a predetermined order of nucleotides to provide to a sequencing reaction, the order being selected to correlate with a predicted sequence for the polynucleotide strand; monitoring the reaction to detect incorporation of a nucleotide into a synthesized polynucleotide strand; wherein, in the event that nucleotide incorporation is detected, proceeding to provide the next nucleotide in the predetermined order. In the event that nucleotide incorporation is not detected, the predicted sequence for the polynucleotide strand may be revised and a new predetermined order of nucleotides selected, wherein the new predetermined order is selected to correlate with the revised predicted sequence. In this way, the sequencing reaction provides feedback to modify the order of nucleotides provided, thereby improving the efficiency of the sequencing reaction.
    • 描述了通过合成测序来测序多核苷酸链的方法,所述方法包括选择预定顺序的核苷酸以提供测序反应,所述顺序被选择为与多核苷酸链的预测序列相关; 监测反应以检测核苷酸掺入合成的多核苷酸链中; 其中,在检测到核苷酸掺入的情况下,继续以预定顺序提供下一个核苷酸。 在未检测到核苷酸掺入的情况下,可以修改多核苷酸链的预测序列,并选择新的预定的核苷酸顺序,其中选择新的预定顺序以与修订的预测序列相关。 以这种方式,测序反应提供反馈以改变提供的核苷酸的顺序,从而提高测序反应的效率。