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    • 85. 发明公开
    • IgG-Fc FRAGMENT AND PROCESS FOR PRODUCING SAME
    • IGG-FC-FRAGMENT UND HERSTELLUNGSVERFAHRENDAFÜR
    • EP2397544A1
    • 2011-12-21
    • EP10741220.7
    • 2010-02-09
    • Otsuka Chemical Co., Ltd.
    • KAJIHARA, YasuhiroFUKAE, Kazuhiro
    • C12N15/09C07K1/04C07K1/113C07K16/00
    • C07K16/462C07K2317/21C07K2317/41C07K2319/00
    • Disclosed is a full-length IgG-Fc fragment having a substantially homogeneous sugar chain added thereto. Also disclosed is a process for producing the full-length IgG-Fc fragment. Specifically disclosed is an IgG-Fc fragment having a sugar chain added thereto, in which the sugar chain is added to the same position as that in a naturally occurring IgG-Fc fragment, any one amino acid residue selected from 1 st to 30 th amino acid residues from the sugar chain-added amino acid residue on the N-terminal side of the sugar chain-added amino acid residue is substituted by a Cys residue and at least one Met residue is substituted by an amino acid residue other than a Met residue. Also specifically disclosed is a process for producing the IgG-Fc fragment.
    • 公开了一种其中加入基本上均匀的糖链的全长IgG-Fc片段。 还公开了生产全长IgG-Fc片段的方法。 具体公开了具有加入糖链的IgG-Fc片段,其中将糖链加入与天然存在的IgG-Fc片段相同的位置,任何一个选自1至30个氨基的氨基酸残基 加入糖链的氨基酸残基的N末端侧的加入糖链的氨基酸残基的酸残基被Cys残基取代,并且至少一个Met残基被除了Met残基之外的氨基酸残基取代 。 还公开了制备IgG-Fc片段的方法。