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71. 发明公开

EP2290058A1 Lipolytic enzyme variants 审中-公开
公开(公告)号：EP2290058A1
公开(公告)日：2011-03-02
申请号：EP10182606.3
申请日：1999-11-29
申请人： Novozymes A/S
发明人： Bojsen, Kirsten , Svendsen, Allan , Fuglsang, Claus Crone , Patkar, Shamkant Anant , Borch, Kim , Vind, Jesper , Petri, Andreas , Glad, Sanne Schroeder , Budolfsen, Gitte
IPC分类号： C12N9/20 , A21D8/04
CPC分类号： C12Y301/01003 , A21D8/042 , C07K2299/00 , C11B3/003 , C11D3/38627 , C12H1/0408 , C12H1/0424 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.

72. 发明公开

EP2278000A1 Lipase variant 审中-公开
标题翻译： Lipasevariante
公开(公告)号：EP2278000A1
公开(公告)日：2011-01-26
申请号：EP10180782.4
申请日：2000-03-30
申请人： Novozymes A/S
发明人： Vind, Jesper , Svendsen, Allan , Patkar, Shamkant Anant , Andersen, Kim V , Halkier, Dorte A , Bojsen, Kirsten
IPC分类号： C12N9/20 , C11D3/386 , C12N5/10
CPC分类号： C11D3/38627 , C12N9/20
摘要： Certain variants of Lipolase (wild-type Humicola lanuginosa lipase) have a particularly good first-wash performance in a detergent solution. The variants should comprise one or more substitutions with positive amino acids near the N-terminal in the three-dimensional structure. The variants should further comprise a peptide addition at the C-terminal and/or should meet certain limitations on electrically charged amino acids at positions 90-101 and 210.
摘要翻译： Lipolase（野生型Humicola lanuginosa脂肪酶）的某些变体在洗涤剂溶液中具有特别好的第一洗涤性能。变体应包含一个或多个在三维结构中N-末端附近具有阳性氨基酸的取代。变体应进一步包含C末端的肽加成和/或应满足90-101和210位带电氨基酸的某些限制。

73. 发明公开

EP2277999A1 Lipase variant 有权
标题翻译：脂肪酶变体
公开(公告)号：EP2277999A1
公开(公告)日：2011-01-26
申请号：EP10180769.1
申请日：2000-03-30
申请人： Novozymes A/S
发明人： Vind, Jesper , Svendsen, Allan , Patkar, Shamkant Anant , Andersen, Kim V , Halkier Dorte A , Bojsen, Kirsten
IPC分类号： C12N9/20 , C11D3/386 , C12N5/10
CPC分类号： C11D3/38627 , C12N9/20
摘要： Certain variants of Lipolase (wild-type Humicola lanuginosa lipase) have a particularly good first-wash performance in a detergent solution. The variants should comprise one or more substitutions with positive amino acids near the N-terminal in the three-dimensional structure. The variants should further comprise a peptide addition at the C-terminal and/or should meet certain limitations on electrically charged amino acids at positions 90-101 and 210.
摘要翻译： Lipolase的某些变体（野生型Humicola lanuginosa脂肪酶）在洗涤剂溶液中具有特别好的第一次洗涤性能。这些变体应该包含一个或多个在三维结构中靠近N端的正氨基酸取代。这些变体还应在C-末端包含肽加成和/或在90-101和210位上对带电氨基酸应该具有某些限制。

74. 发明公开

EP2267108A1 Enzymatic oil-degumming method 无效转让
标题翻译：酶促油脱胶法
公开(公告)号：EP2267108A1
公开(公告)日：2010-12-29
申请号：EP10174207.0
申请日：2005-07-18
申请人： Danisco A/S
发明人： Soe, Jørn Borch , Turner, Mark
IPC分类号： C11B3/00 , C11C3/00 , C07F9/10
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： A process of enzymatic degumming edible oils, comprising treating the edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxy group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprises one or more of the following amino acid sequences: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, or SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID No. 16 is also taught.
摘要翻译：一种酶促脱胶食用油的方法，包括用脂质酰基转移酶处理食用油以便将酰基从大部分磷脂转移到一个或多个酰基受体，其中酰基受体可以是任何含有羟基的化合物。在一个实施方案中，优选酰基受体是水，在另一个实施方案中，优选酰基受体是一种或多种甾醇和/或甾烷醇。当酰基受体是甾烷醇和/或甾醇时，产生一种或多种甾醇酯和/或甾烷醇酯。用于本发明方法的脂质酰基转移酶可以包含一个或多个以下氨基酸序列：SEQ ID No.1，SEQ ID No.3，SEQ ID No.4，SEQ ID No.5，SEQ ID No.5 序列号6，序列号7，序列号8，序列号9，序列号10，序列号11，序列号12，序列号13，序列号 14或SEQ ID No.15，SEQ ID No.16，SEQ ID No.17，SEQ ID No.18，SEQ ID No.36，SEQ ID No.38，SEQ ID No.40，SEQ ID No.41 ，SEQ ID No 45，SEQ ID No.47，SEQ ID No.50或与其具有75％或更多同一性的氨基酸序列。还教导了包含如SEQ ID No.16所示的氨基酸序列的新型脂质酰基转移酶。

75. 发明公开

EP2264159A2 Lipolytic enzyme variants 有权
标题翻译：脂多糖酶维生素
公开(公告)号：EP2264159A2
公开(公告)日：2010-12-22
申请号：EP10182311.0
申请日：2007-11-26
申请人： Novozymes A/S
发明人： De Maria, Leonardo , Brask, Jesper , Skjøt, Michael , Patkar, Shamkant Anant , Borch, Kim , Svendsen, Allan
IPC分类号： C12N9/20 , C12P7/40
CPC分类号： C12N9/20 , C12P7/40 , C12P7/62 , C12P7/6418 , C12Y301/01003
摘要： Molecular dynamics (MD) simulation on the three-dimensional structure of Candida anrtarctica lipase B revealed two hitherto unknown lids with a marked mobility, and this discovery was used to design lipolytic enzyme variants with increased lipolytic enzyme activity.
摘要翻译：假丝酵母脂肪酶B的三维结构的分子动力学（MD）模拟显示两个具有显着迁移率的未知盖，并且该发现用于设计具有增加的脂肪分解酶活性的脂肪分解酶变体，如其中残基223具有被推翻了

76. 发明公开

EP2264158A2 Lipolytic enzyme variants 审中-公开
标题翻译：脂多糖酶维生素
公开(公告)号：EP2264158A2
公开(公告)日：2010-12-22
申请号：EP10182292.2
申请日：2007-11-26
申请人： Novozymes A/S
发明人： De Maria, Leonardo , Brask, Jesper , Skjøt, Michael , Patkar, Shamkant Anant , Borch, Kim , Svendsen, Allan
IPC分类号： C12N9/20 , C12P7/40
CPC分类号： C12N9/20 , C12P7/40 , C12P7/62 , C12P7/6418 , C12Y301/01003
摘要： Molecular dynamics (MD) simulation on the three-dimensional structure of Candida anrtarctica lipase B revealed two hitherto unknown lids with a marked mobility, and this discovery was used to design lipolytic enzyme variants with increased lipolytic enzyme activity.
摘要翻译：假丝酵母假丝酵母脂肪酶B的三维结构的分子动力学（MD）模拟显示两个具有显着移动性的未知盖，并且该发现用于设计具有增加的脂肪分解酶活性的脂肪分解酶变体，例如残基285具有的变体被改变了

77. 发明公开

EP2261328A1 Lipase variants for pharmaceutical use 有权转让
标题翻译：脂肪酶变体zur pharmazeutischen Verwendung
公开(公告)号：EP2261328A1
公开(公告)日：2010-12-15
申请号：EP10172609.9
申请日：2007-12-12
申请人： Novozymes A/S , Novozymes Inc. , Abbott Products GmbH
发明人： Svendsen, Allan , Skjoet, Michael , Yaver, Debbie , Christensen, Lars Lehmann Hylling , Larsen, Signe , Lundin, Tina , Lamse, Michael , Gregory, Peter Colin
IPC分类号： C12N9/20 , A61K38/46 , A61K38/54
CPC分类号： A61K38/465 , A61K38/47 , A61K38/48 , C12N9/20
摘要： The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, with at least substitutions N33Q, T231R, N233R and D254S optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.
摘要翻译：与包含SEQ ID NO：2的氨基酸1-269，至少具有取代N33Q，T231R，N233R和D254S的Thermomyces lanuginosus（Humicola lanuginosa）脂肪酶相关的脂肪酶的药物用途任选与蛋白酶和/或淀粉酶组合。医学适应症的例子有：消化系统疾病，胰腺外分泌功能不全（PEI），胰腺炎，囊性纤维化，I型糖尿病和/或II型糖尿病的治疗。本发明的脂肪酶具有例如体外改善的消化性能，在中性范围内的pH值改善的活性，在低pH下的改善的稳定性，对蛋白酶降解是稳定的，和/或在存在胃蛋白酶和胆汁盐。本发明还涉及确定脂肪酶的体外消化性能的方法以及T.lanuginosus的脂肪酶的某些新颖变体。

78. 发明公开

EP2258853A1 Lipolytic enzyme variant 有权
标题翻译：变异体脂肪分解酶
公开(公告)号：EP2258853A1
公开(公告)日：2010-12-08
申请号：EP10178382.7
申请日：2001-04-30
申请人： Novozymes A/S
发明人： Roggen, Erwin Ludo
IPC分类号： C12N15/55 , C12N9/20 , C11D3/386 , A21D8/04
CPC分类号： C12N9/54 , A21D2/267 , A21D8/042 , A61K39/00 , C07K1/047 , C07K5/1008 , C07K5/101 , C07K5/1013 , C07K5/1019 , C07K5/1021 , C07K14/001 , C07K16/40 , C07K16/4283 , C11D3/38627 , C11D3/38645 , C12N9/0061 , C12N9/20 , C12Y110/03002 , C12Y304/21062 , Y10S435/911
摘要： The properties of a fungal lipolytic enzyme can be altered by substituting amino acid residues corresponding to certain specified amino acid residues in the T. lanuginosus lipase. The altered property may be, e.g., an increased thermostability, an altered pH dependence, or an altered substrate specificity.
摘要翻译：真菌脂肪分解酶的性质可以通过替换对应于T.lanuginosus脂肪酶中某些特定氨基酸残基的氨基酸残基来改变。改变的性质可以是例如增加的热稳定性，改变的pH依赖性或改变的底物特异性。

79. 发明公开

EP2245146A1 NEW CALB MUTEINS AND THEIR USE 有权
标题翻译：新CALB突变蛋白及其用途
公开(公告)号：EP2245146A1
公开(公告)日：2010-11-03
申请号：EP08865769.7
申请日：2008-12-18
申请人： BASF SE
发明人： HAUER, Bernhard , KVARNSTROEM BRANNEBY, Cecilia , SCHMID, Rolf , MAURER, Steffen, Christian , TRODLER, Peter , LIU, Danni , MÜLLER, Monika
IPC分类号： C12N9/20 , C12P7/62
CPC分类号： C12N9/20 , C12P7/62
摘要： A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.

80. 发明授权

EP1713930B1 RAPID SCREENING METHOD OF TRANSLATIONAL FUSION PARTNERS FOR PRODUCING RECOMBINANT PROTEINS AND TRANSLATIONAL FUSION PARTNERS SCREENED THEREFROM 有权
标题翻译：用于生产重组蛋白快速筛选过程翻译融合合作伙伴通过甄别订单已收到翻译融合伙伴
公开(公告)号：EP1713930B1
公开(公告)日：2010-11-03
申请号：EP04808645.8
申请日：2004-12-30
申请人： Korea Research Institute of Bioscience and Biotechnology
发明人： SOHN, Jung-Hoon , CHOI, Eui-Sung , BAE, Jung-Hoon , LEE, Eung-Suck , SHIN, Mi-Kyung
IPC分类号： C12N15/62 , C12Q1/34
CPC分类号： C12P21/02 , C07K14/535 , C07K14/55 , C07K2319/00 , C07K2319/35 , C07K2319/61 , C12N9/20 , C12N15/1086
摘要： Disclosed are a method for rapid screening of suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.

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