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41. 发明授权

EP0794435B1 Cyanide-free reagent and method for hemoglobin determination and leukocyte differentiation 失效
标题翻译：氰化物试剂和方法，用于血红蛋白和白细胞的测定
公开(公告)号：EP0794435B1
公开(公告)日：2003-01-02
申请号：EP96301568.0
申请日：1996-03-07
申请人： Coulter International Corp.
发明人： Riesgo, Mirta I. , Young, Carole J.
IPC分类号： G01N33/72 , G01N33/50
CPC分类号： G01N33/5094 , G01N33/721

42. 发明授权

EP1058831B1 SELECTIVE PURIFICATION AND ENRICHMENT SORTING OF FLOW CYTOMETER DROPLETS BASED UPON ANALYSIS OF DROPLET PRECURSOR REGIONS 有权
标题翻译：选择性浓度和富集分选FLUSSZYTOMETERTROPFEN基于对液滴前驱物区域分析
公开(公告)号：EP1058831B1
公开(公告)日：2002-05-08
申请号：EP99906853.9
申请日：1999-02-09
申请人： Coulter International Corp.
发明人： KIROUAC, William, E. , STATLER, Eric , STARLING, J., David
IPC分类号： G01N15/14
CPC分类号： G01N15/14 , G01N15/1404 , G01N2015/149
摘要： A flow cytometer sorting mechanism is operative to controllably sort successively generated fluid droplets in accordance with the contents of a plurality of contiguous precursor regions of the carrier fluid which are contained within a target droplet of interest and precursor regions for droplets on either side of the target droplet. This multi-region sorting window scheme is particularly useful in reducing the time required to harvest a highly purified quantity of a particular type of cell, as not only are droplets containing only desired cells sorted to a highly purified collection store, but should an undesired cell be detected within a predefined proximity of a desired cell, rather than being simply aborted, the droplet is sorted into an auxiliary enrichment container, whose contents may then be reclaimed for resorting. Since all droplets are sorted to one of a plurality of droplet reclamation containers, substantially no detected/target cells are lost.

43. 发明公开

EP1023602A1 PROTEIN-COLLOIDAL METAL-AMINODEXTRAN COATED PARTICLE AND METHODS OF PREPARATION AND USE 有权
标题翻译：金属包覆颗粒PROTEINKOLLOIDALEN及其制造方法
公开(公告)号：EP1023602A1
公开(公告)日：2000-08-02
申请号：EP98949745.8
申请日：1998-10-01
申请人： Coulter International Corp.
发明人： SIIMAN, Olavi , GORDON, Kristie , RODRIGUEZ, Carlos, M. , BURSHTEYN, Alexander , MAPLES, John, A. , WHITESELL, James, Keller
IPC分类号： G01N33/553 , G01N33/543 , G01N33/548 , G01N33/50
CPC分类号： G01N33/5094 , G01N33/54313 , G01N33/54353 , G01N33/54393 , G01N33/548 , G01N33/553 , Y10T428/2982 , Y10T428/2991
摘要： A stable colloidal particle comprises a colloidal-sized core substrate having amine-reactive functional groups thereon with an aminodextran coating over its peripheral surface and a layer of colloidal-sized metallic solid overlaying the aminodextran coating. A linker attached to the metallic solid has a free amino group to which a protein is attached by covalent bonding to the free amino group. Such novel particles are useful in flow cytometry, particularly useful in the simultaneous analyses of subpopulations of leukocytes. Methods of preparation of such particles, as well as methods of use are provided. These conjugates enable the simultaneous analyses of at least two different mutually exclusive subsets of white blood cells, based on the binding affinity of the conjugated protein.

44. 发明公开

EP0948254A1 CELL CONTROL USED TO CONFIRM ENZYMATIC ACTIVITY 失效
标题翻译： CELL测试，以确认酶活性
公开(公告)号：EP0948254A1
公开(公告)日：1999-10-13
申请号：EP97943650.0
申请日：1997-10-01
申请人： Coulter International Corp.
发明人： SHUKLA, Ravindra, S. , CREWS, Harold, R. , GALIOUNGHI, Adry , LANDRUM, Eileen , LUCAS, Francis, J.
IPC分类号： G01N33 , A01N1 , C12N5 , C12N9
CPC分类号： G01N33/5005 , Y10T436/10
摘要： The invention provides a novel cell control product for use with a cytoenzymology assay to confirm enzymatic activity in devices employing electronic and/or optical means. The cell control product comprises a lyophilized mammalian cell which is capable of being rehydrated in water to exhibit cellular structure and cellular enzymatic activity so that said lyophilized cells can function effectively as a cytoenzymology cell control in an enzymatic analysis. The cellular structure of the cell control is capable of being authenticated by light scatter or microscopy analysis and the cellular enzymatic activity is capable of being authenticated by fluorescence analysis. The cell control exhibits a real time stability when stored at between 2 and 8 °C for at least a two-month period of time. Preferably, the mammalian cell is an abnormal cell selected from Molt 4, CEM and HL 60 cell lines.

45. 发明公开

EP0861431A1 BLOOD ANALYSIS SYSTEM HAVING BLOOD STORAGE, TRANSPORT AND AUTOMATIC SLIDE-MAKING CAPABILITIES 失效
标题翻译：系统用于血液分析，随着对存储和血液运输和自动产生血样阶表示
公开(公告)号：EP0861431A1
公开(公告)日：1998-09-02
申请号：EP96937023.0
申请日：1996-10-25
申请人： Coulter International Corp.
发明人： SPERBER, Cynthia, J. , GAO, Daniel, Dashui , GRAHAM, Marshall, D.
IPC分类号： G01N33 , G01N1 , G01N35
CPC分类号： G01N1/2813 , G01N2035/00544 , Y10T436/11 , Y10T436/112499 , Y10T436/114998 , Y10T436/117497 , Y10T436/25 , Y10T436/2575
摘要： A blood analyzer system is provided with improved slide-making capability by coupling a blood analyzer apparatus (20) to an automatic slide-making apparatus (40) by means of a clinically effective blood specimen transport assembly (60), which includes a blood conduit forming a curvilinear flow path that imparts a substantial angular velocity component to blood moving therein. The system enables a blood analyzer portion and a smear slide portion of a given sample to be aspirated with physical and temporal proximity.

46. 发明公开

EP0832279A1 AN ASSAY REAGENT AND A METHOD OF MAKING AND USING THE ASSAY REAGENT 失效
标题翻译：化学试剂和工艺生产和使用
公开(公告)号：EP0832279A1
公开(公告)日：1998-04-01
申请号：EP96915790.0
申请日：1996-05-14
申请人： Coulter International Corp.
发明人： LUCAS, Frank, J. , JAFFE, Gerald, E. , BOTT, Steven, E. , CARTER, James, H.
IPC分类号： G01N33 , C12Q1
CPC分类号： C12Q1/34 , C12Q2334/40 , C12Q2337/40 , G01N33/574
摘要： An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.

47. 发明公开

EP0760946A1 APPARATUS FOR MEASURING ANALYTES IN A FLUID SAMPLE 失效
标题翻译：设备技术分析物在样品FLUSSIGEN测定
公开(公告)号：EP0760946A1
公开(公告)日：1997-03-12
申请号：EP96910565.0
申请日：1996-03-26
申请人： Coulter International Corp.
发明人： AZARNIA, Roobik , FUENTES, Susan , RUSSEL, Thomas
IPC分类号： G01N27 , C12M1 , C12Q1
CPC分类号： C12Q1/006 , C12Q1/00 , G01N27/3271 , Y10S435/817 , Y10T436/117497
摘要： An apparatus (10) for measuring an analyte in a fluid sample (12) comprises a housing (H) defining a pair of chambers (26, 30) separated by a semipermeable membrane (28). The membrane allows the analyte of interest to pass between the chambers. The second chamber (30) contains a detector adapted to either (a) directly detect the analyte of interest or (b) to detect a reaction by product of the analyte upon reacting with a substance (14) such as an enzyme, introduced into the second chamber. Means are provided to flush the second chamber after each analyte measurement.

48. 发明公开

EP1540300A1 APPARATUS FOR DETECTING BACK-SCATTER IN A LASER-BASED BLOOD ANALYSIS SYSTEM 审中-公开
标题翻译： DEVICE对于R CKSTREUUNG的在血液分析系统的基于激光的检测
公开(公告)号：EP1540300A1
公开(公告)日：2005-06-15
申请号：EP03793018.7
申请日：2003-08-06
申请人： Coulter International Corp.
发明人： KRAMER, Donald, L.
IPC分类号： G01N1/00
CPC分类号： G01N15/1459 , G01N21/474 , G01N2015/1006
摘要： A back-scatter detector for detecting light scattered backwardly (i.e., reflected) by an irradiated particle such as a blood cell comprises a plurality of optical fibers. A fiber optic holder (30) having a centrally located opening for passing a light beam used to irradiate particles at a particle-interrogation zone serves to position the light-collecting ends at a desired position to collect back-scattered light. Preferably, the light-collecting ends of the optical fibers are positioned in a circular pattern centered about the irradiating light beam, and the respective axes of the supported portions of the optical fibers extend either parallel to the beam axis, or, more preferably, so that they converge at or near the location of the scattering source, i.e., the irradiated particle or cell. The latter configuration assures that the back-scatter light enters the fiber end substantially parallel to the fiber axis, thereby reducing optical transmission loses in the fiber.

49. 发明授权

EP1049919B1 METHOD FOR DIFFERENTIATION OF NUCLEATED RED BLOOD CELLS 有权
标题翻译：为了分化红细胞核方法
公开(公告)号：EP1049919B1
公开(公告)日：2005-04-27
申请号：EP99901329.5
申请日：1999-01-13
申请人： Coulter International Corp.
发明人： LI, Yi , LI, Jing , YOUNG, Carole
IPC分类号： G01N15/14
CPC分类号： G01N15/1456 , G01N33/80
摘要： A method is provided for differentiation of nucleated red blood cells. In addition, the method provides for a concurrent differentiation of leukocytes in a blood cell sample by suitable electronic and optical measurements. The method includes exposing a blood cell sample to a reagent system to lyse mature red blood cells and subsequently analyzing nucleated red blood cells in a flow cell by optical analysis. A concurrent differentiation of nucleated blood cells and leukocytes can be performed using electronic and optical analysis. The electronic and optical analysis includes light scatter and impedance measurements. This method eliminates the use of nuclear stain for identification of nucleated red blood cells. The method of the present invention, for the first time, reports differentiation and enumeration of nucleated red blood cells without using fluorescence.

50. 发明公开

EP1298441A3 A cyanide-free lytic reagent composition and method for hemoglobin and cell analysis 失效
标题翻译：氰化物溶解剂和Vefahren血红蛋白和细胞分析
公开(公告)号：EP1298441A3
公开(公告)日：2004-08-11
申请号：EP02028716.5
申请日：1998-01-13
申请人： Coulter International Corp.
发明人： Li, Yi , Young, Carole
IPC分类号： G01N33/72 , G01N33/50
CPC分类号： G01N33/721 , G01N33/5094 , G01N33/56972 , Y10T436/10 , Y10T436/101666 , Y10T436/105831 , Y10T436/107497 , Y10T436/108331 , Y10T436/25125
摘要： A cyanide-free lytic reagent composition and method for measuring the total hemoglobin concentration in a blood sample, for counting the number of leukocytes and for differential counting of three leukocyte subpopulations including lymphocytes, monocytes, and granulocytes are described. The cyanide-free lytic reagent composition comprises a hemolytic surfactant selected from the group consisting of quaternary ammonium salts, pyridinium salts, organic phosphate esters, and alkyl sulfonates to lyse erythrocytes and release hemoglobin, and an organic ligand selected from the group consisting of triazole and its derivatives, tetrazole and its derivatives, alkaline metal salts of oxonic acid, melamine, aniline-2-sulfonic acid, quinaldic acid, 2-amino-1,3,4-thiadiazole, triazine and its derivatives, urazole, DL-pipecolinic acid, isonicotinamide, anthranilonitrile, 6-aza-2-thiothymine, adenine, 3-(2-thienyl)acrylic acid, benzoic acid and alkali metal and ammonium salts of benzoic acid, and pyrazine and its derivatives to form a stable chromogen with hemoglobin. The lytic reagent composition has a pH ranging from about 1 to about 13. The lytic reagent composition is mixed with a blood sample which is prediluted with a suitable blood diluent and the UV absorption of the sample mixture is measured at the predetermined absorption wavelength of the formed chromogen from 510 nm to 560 nm. Counting the numbers of leukocytes and differential counting of three leukocyte subpopulations are accomplished simultaneously on an automated cell counter utilizing DC impedance measurement. Alternatively, the organic ligands can be added to a suitable blood diluent for hemoglobin and leukocyte analysis.

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