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21. 发明公开

EP0256806A3 Method and apparatus for the determination of analytes 有权转让
标题翻译：用于确定分析物的方法和装置
公开(公告)号：EP0256806A3
公开(公告)日：1988-11-23
申请号：EP87307014
申请日：1987-08-07
申请人： LIFESCAN, INC.
发明人： Phillips, Roger , Underwood, Ray , McGarraugh, Geoffrey , Jurik, Frank
IPC分类号： C12Q01/54 , G01N33/66 , C12Q01/00 , G01N33/52 , G01N21/47
CPC分类号： G01N21/8483 , C12Q1/54 , G01N21/314 , G01N21/4738 , G01N33/52 , G01N33/521 , G01N33/523 , G01N33/66 , G01N35/1016 , G01N2021/4757 , G06F19/00 , Y02A90/26 , Y10S435/805 , Y10S435/808 , Y10S435/81 , Y10T436/112499 , Y10T436/144444
摘要： A method for determining the presence of an analyte in a fluid is described along with an apparatus specifically designed to carry out the method. The method involves taking a reflectance reading from one surface (15) of an inert porous matrix (11) impregnated with a reagent that will interact with the analyte to produce a light-absorbing reaction product when the fluid being analysed is applied to another surface (16) and migrates through the matrix to the surface being read. Reflectance measurements are made at two separate wavelengths in order to eliminate interferences, and a timing circuit is triggered by an initial decrease in reflectance by the wetting of the surface whose reflectance is being measured by the fluid which passes through the inert matrix. The method and apparatus are particularly suitable for the measurement of glucose levels in blood without requiring separation of red blood cells from serum or plasma.

22. 发明公开

EP0229709A3 Improvements in or relating to the chemical analysis of tabacco or smoking related products 失效
标题翻译：化学分析或相关产品化学分析的改进
公开(公告)号：EP0229709A3
公开(公告)日：1988-02-24
申请号：EP87300253
申请日：1987-01-13
申请人： IMPERIAL TOBACCO LIMITED
发明人： Long, Terence Michael , Alsop, Susan Jane , Newman, David James
IPC分类号： G01N33/00 , C12Q01/54
CPC分类号： G01N31/22 , C12Q1/34 , C12Q1/54 , G01N2333/904 , G01N2333/9121 , Y10T436/200833 , Y10T436/201666 , Y10T436/203332
摘要： A method of determining in a discrete analyzer the total sugars content of a tobacco sample containing sucrose, glucose and fructose, comprises the steps of, converting the sucrose to glucose and fructose, enzymatically converting the glucose and fructose to glucose-6-phosphate in the presence of hexokinase, reacting the glucose-6-phosphate with nicotinamide-adenine dinucleotide in the presence of glucose-6-phosphate dehydrogenase to give reduced nicotinamide-adenine dinucleotide, the amount of reduced nicotinamide-adenine dinucleotide produced being directly proportional to the total sugars content of the sample, measuring the concentration of reduced nicotinamide-adenine dinucleotide in the sample, and generating a signal indicative of the concentration of reduced nicotinamide-adenine dinucleotide and hence of the total sugars content of the sample. Deactivation of enzymes by any cyanogen bromide that may be present is prevented by the addition of dithioerythritol to the reaction mixture.

23. 发明公开

EP0207360A3 Diagnostic test device 失效转让
标题翻译：诊断测试装置
公开(公告)号：EP0207360A3
公开(公告)日：1988-01-07
申请号：EP86108238
申请日：1986-06-16
申请人： MILES LABORATORIES, INC.
发明人： Jones, James E.
IPC分类号： G01N33/52 , C12Q01/54
CPC分类号： C12Q1/54 , G01N33/525
摘要： A diagnostic test device for detecting an analyte in fluids, particularly body fluids such as blood, includes bibulous material impregnated with at least one ingredient capable of producing a detectable response upon contact with the fluid. The bibulous material may form a substrate for the teat device or It may be in the form of pads mounted on a rigid matrix or substrate. A selectively permeable silicone film is bonded over the bibulous material and to the substrate. Upon curing, the silicone water based elastomer forms a durable rubbery surface that can be wiped without tearing or damaging the film. In the precured state the silicono water based elastomer ie liquid and can be easily applied over the bibulous material. If the bibulous material is formed as pads on a substrate, the precured elastomer will flow over and between the pads and the curad elastomer film will bond to the substrate preventing runover between the pads.

24. 发明公开

EP0206316A3 Method and test composition for determination of hydrogen peroxide 失效
标题翻译：用于确定过氧化氢的方法和测试组合物
公开(公告)号：EP0206316A3
公开(公告)日：1987-11-25
申请号：EP86108596
申请日：1986-06-24
申请人： Kyowa Medex Co. Ltd.
发明人： Aoyama, Norihito , Okano, Mikio , Miike, Akira , Tatano, Toshio
IPC分类号： C12Q01/28 , C12Q01/26 , C12Q01/62 , C12Q01/60 , C12Q01/54
CPC分类号： C12Q1/28 , C12Q2326/40 , Y10S436/904 , Y10T436/206664
摘要： Disclosed is a method for the colorimetric determination of hydrogen peroxide in a sample by reacting a particular chromogen with the hydrogen peroxide in the presence of peroxidase and measuring the absorbancy of the reaction solution in the visible ray region. Also disclosed is a test composition for carrying out the determination.

25. 发明公开

EP0193061A3 Viability test device 失效转让
标题翻译：可变性测试设备
公开(公告)号：EP0193061A3
公开(公告)日：1987-08-12
申请号：EP86101968
申请日：1986-02-17
申请人： MILES LABORATORIES, INC.
发明人： Hemmes, Paul , Ismail, Ibrahim A. , Skarstedt, Mark T. , Zipp, Adam
IPC分类号： G01N33/96 , G01N33/52 , C12Q01/54 , C12Q01/00
CPC分类号： G01N33/521 , C12Q1/54 , Y10T436/104998
摘要： A viability test device wherein the viability of a test composition for the determination of an analyte of interest can be determined by simply wetting the device with ordinary water. The viability device can be prepared in three formats: a control or calibrator device, an internal control device and a self indicating device. Unreacted analyte, or an analog thereof, can be incorporated in a limited defined portion of a dried carrier matrix incorporated with a test composition to provide an internal control test device. When wetted with water, a positive optical response, usually color, indicates the test composition can provide a viable test for the analyte. The internal control test device is particularly advantageously used by diabetics in their own homes where a negative test response could be due to the desired control of the user's condition or due to deterioration of a test composition because of unfavourable storage conditions.

26. 发明公开

EP0171150A3 Method and apparatus for assaying with optional reagent quality control 有权
标题翻译：用于测试可选试剂质量控制的方法和装置
公开(公告)号：EP0171150A3
公开(公告)日：1987-07-01
申请号：EP85304197
申请日：1985-06-12
申请人： Orgenics Ltd.
发明人： Herzberg, Max , Fish, Falk
IPC分类号： G01N33/543 , C12Q01/28 , C12Q01/54
CPC分类号： B01L3/508 , G01N33/528 , G01N33/54366
摘要： A solid phase immuno-assay system and method for detecting and measuring at least one analyte in a single sample. The system includes a solid support having a plurality of receptors bound thereto. The support may be selectively treated whereby upon exposure to a sample, and after subsequent processing, the support exhibits a standard reference curve which may be compared with another portion of the card for reference purposes. Alternatively, or additionally, the support may be selectively treated with a control substance to monitor the activity of at least one reagent in the procedure. An apparatus which may be used for performing the multi-step assay procedure which includes a container having a plurality of compartments therein, each containing a reagent for the assay. A method of detecting the presence of horseradish peroxidase by contacting a substance suspected of containing horseradish peroxidase with potassium iodide, starch and hydrogen peroxide.

27. 发明公开

EP0125136A3 Assay systems utilising more than one enzyme 有权转让
标题翻译：测定系统使用超过一个ENZYME
公开(公告)号：EP0125136A3
公开(公告)日：1987-01-14
申请号：EP84303086
申请日：1984-05-08
申请人： Genetics International, Inc.
发明人： Davis, Graham , Hill, Hugh Allen Oliver
IPC分类号： C12Q01/00 , C12Q01/48 , C12Q01/54
CPC分类号： A61B5/1486 , A61B5/6848 , C12Q1/001 , C12Q1/004 , C12Q1/6825 , C12Q2565/607
摘要： This specification discloses assay systems which detect the presence of, or measure or monitor the extent of, an enzyme-catalysed reaction on a substrate. The systems of the present invention can be used to detect or measure the enzyme or the substrate, depending upon circumstances. The specification further discloses a method of assay of the type in which an electrode (1) poised at a suitable potential is contacted with a system comprising a first enzyme, (GO) a substrate (G) which undergoes a reaction catalysed by the said enzyme, and a mediator compound (F) which transfers charge to the electrode (1) from the first enzyme (GO) when it is catalytically active, whereby the current flowing in the electrode is a measure of the reaction taking place; in which at least one further enzyme (HK) and associated compound (ATP) is incorporated into the system, the further enzyme (HK) being productive of, or also being reactive with, the substrate (G) so as to affect its presence or level, but not being electrochemically linked by the mediator (F) to the electrode (1), whereby the consequent difference in electrode current flowing with, and in the absence of, the second enzyme (HK) and its associated compound is a measure of the extent of reaction of the further enzyme with its associated compound and thus permits the amount of one to be established if the amount of the other is known. There are two main subdivisions of the present disclosure (i) in which the electrode is provided at its surface with the first enzyme and the mediator compound to constitute a sensor electrode and (ii) in which the further enzyme exerts a catalytic change on a specific further substrate to yield substrateforthe first enzyme.

28. 发明公开

EP0120220A3 Method and reagent for the determination of glucose in haemolised blood 有权
标题翻译：用于确定血液中葡萄糖的方法和试剂
公开(公告)号：EP0120220A3
公开(公告)日：1986-01-29
申请号：EP84100965
申请日：1984-01-31
申请人： BOEHRINGER MANNHEIM GMBH
发明人： Draeger, Brigitte, Dr. , Ziegenhorn, Joachim, Dr.
IPC分类号： C12Q01/54
CPC分类号： C12Q1/54

29. 发明公开

EP0105413A3 Cell detection by measurement of flavin adenine dinucleotide 失效
标题翻译：通过测量FLAVIN ADENINE DINUCLEOTIE进行细胞检测
公开(公告)号：EP0105413A3
公开(公告)日：1984-12-05
申请号：EP83109420
申请日：1983-09-22
申请人： MILES LABORATORIES, INC.
发明人： Morris, David Lindsay
IPC分类号： C12Q01/68 , C12Q01/54
CPC分类号： C12Q1/04 , C12Q1/54
摘要： A method for detecting cellular material, e.g., microbial cells or debris from ruptured microbial cells, in a liquid test sample by determining flavin adenine dinucleotide (FAD) in the liquid sample. Comparing the amount of FAD determined in the test sample to that present in a reference sample, e.g., containing no significant amount of relevant cellular material, enables detection of cellular material of interest in the sample. The method is particularly applicable to the detection of microbial contamination in biological fluids and is preferably carried out by measuring FAD with an apo(glucose oxidase) assay composition. Test systems, kits and devices are also provided.

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