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21. 发明公开

EP2875153A1 SENSING APPARATUS AND METHOD 有权转让
标题翻译：扫描程序
公开(公告)号：EP2875153A1
公开(公告)日：2015-05-27
申请号：EP13740352.3
申请日：2013-07-18
申请人： DNA Electronics Ltd
发明人： ANSARI, Zahid , AMIN, Krishna , JORGENSEN, Ginny , KOLB, Kurt , MORLEY, Daniel , PATEL, Alpesh , REED, Sam , SHEPHERD, Leila , TOUMAZOU, Christofer
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2527/101 , C12Q2527/119 , C12Q2535/122 , C12Q2543/101 , C12Q2547/101 , C12Q2565/537 , C12Q2565/629
摘要： Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.

22. 发明公开

EP2774999A1 Synthesis of long FISH probes 审中-公开
标题翻译： Synthese von langen FISH-Sonden
公开(公告)号：EP2774999A1
公开(公告)日：2014-09-10
申请号：EP14153591.4
申请日：2014-02-03
申请人： Agilent Technologies, Inc.
发明人： Leproust, Emily Marine , Chen, Siyuan , Ruvolo, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q1/6883 , C12Q1/6886 , G06F19/20 , C12Q2531/113 , C12Q2533/107 , C12Q2543/101
摘要： A method comprising: synthesizing a set of overlapping oligonucleotides that comprises probe sequences that hybridize to unique sequences in a chromosome, assembling the overlapping oligonucleotides in a way that produces one or more double stranded polynucleotides that each comprises multiple probe sequences, labeling the one or more double stranded polynucleotides to produce one or more labeled probes, and hybridizing the labeled probes to an intact chromosome, in situ , is provided.
摘要翻译：一种方法，包括：合成一组重叠的寡核苷酸，其包含与染色体中的唯一序列杂交的探针序列，以产生一个或多个双链多核苷酸的方式组装重叠的寡核苷酸，每个双链多核苷酸各自包含多个探针序列，标记所述一个或多个提供双链多核苷酸以产生一个或多个标记的探针，并将标记的探针与原位染色体杂交。

23. 发明授权

EP2029745B1 DIAGNOSTIC METHODS AND MARKERS 有权
标题翻译：诊断过程后标记
公开(公告)号：EP2029745B1
公开(公告)日：2011-10-12
申请号：EP07808640.2
申请日：2007-06-07
申请人： Otago Innovation Limited
发明人： ASSINDER, Stephen, John , STANTON, Jo-Ann
IPC分类号： C12Q1/68
CPC分类号： C07K14/4748 , C12Q1/6816 , C12Q1/6886 , G01N33/5023 , G01N33/57434 , G01N2500/00 , G01N2800/52 , C12Q2543/101
摘要： The present invention relates to methods of detecting, monitoring and treating prostate cancer (PRC) OR prostatic intraepithelial neoplasia (PIN) or a predisposition to same. Provided for use in the methods is a novel cancer marker, PSPU43, as well as bioassays and kits.

24. 发明授权

EP1300466B1 A cascade nucleic acid amplification reaction 失效
标题翻译：延伸在核酸扩增级联反应
公开(公告)号：EP1300466B1
公开(公告)日：2006-04-26
申请号：EP03000499.8
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131

25. 发明授权

EP0884394B1 Apparatus for assembly of an in situ PCR sample and reagent fluid containment system on a glass slide 失效
标题翻译：装置用于在薄片原位PCR构建一个样品和试剂接收系统，用于
公开(公告)号：EP0884394B1
公开(公告)日：2005-03-23
申请号：EP98115093.1
申请日：1993-09-30
申请人： Applera Corporation
发明人： Atwood, John G. , Haff, Larry
IPC分类号： C12Q1/68 , G02B21/34 , B01L7/00 , G01N35/00
CPC分类号： G01N1/312 , B01J19/0046 , B01J2219/00722 , B01L3/508 , B01L7/52 , B01L9/52 , B01L2200/0684 , B01L2200/0689 , B01L2300/041 , B01L2300/0822 , B01L2300/1827 , B01L2300/185 , C12Q1/6841 , C12Q1/686 , C40B40/06 , Y10S435/809 , C12Q2543/101

26. 发明授权

EP0826066B1 METHODS AND REAGENTS FOR COMBINED PCR AMPLIFICATION AND HYBRIDIZATION PROBING ASSAY 失效
标题翻译：方法和试剂用于PCR扩增与杂交分析相结合的
公开(公告)号：EP0826066B1
公开(公告)日：2000-09-13
申请号：EP96911592.2
申请日：1996-04-05
申请人： THE PERKIN-ELMER CORPORATION
发明人： MAYRAND, Paul E.
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6818 , C07H21/04 , C12Q1/6853 , C12Q1/686 , Y10S435/81 , Y10S435/911 , C12Q2565/107 , C12Q2565/1015 , C12Q2525/186 , C12Q2525/125 , C12Q2543/101 , C12Q2537/101 , C12Q2563/113 , C12Q2531/113 , C12Q2527/101 , C12Q2521/513
摘要： An oligonucleotide probe is disclosed, the probe including an oligonucleotide, a fluorescer molecule attached to a first end of the oligonucleotide and a quencher molecule attached to the opposite end of the oligonucleotide. The probe is rendered impervious to digestion by the 5'→3' exonuclease activity of a polymerase and the 5'→3' extension by a polymerase. The invention also includes methods for performing combined PCR amplification and hybridization probing, one such method including the steps of contacting a target nucleic acid sequence with PCR reagents and an oligonucleotide probe as described above, and subjecting these reagents to thermal cycling. One preferred refinement of the above method further includes the addition of a strand displacer to facilitate amplification. Additional similar combined PCR hybridization methods are disclosed, such methods not requiring probes having their 5' ends protected, wherein (i) the polymerase lacks 5'→3' exonuclease activity, (ii) a 5'→3' exonuclease inhibitor is included, and (iii) an exonuclease deactivation step is performed.

27. 发明授权

EP0611598B1 In situ PCR amplification system 失效
标题翻译：在原位PCR扩增程序系统
公开(公告)号：EP0611598B1
公开(公告)日：1999-03-03
申请号：EP93115813.3
申请日：1993-09-30
申请人： THE PERKIN-ELMER CORPORATION
发明人： Atwood, John G. , Haff, Larry
IPC分类号： B01L7/00 , B01L3/00 , B01J15/00
CPC分类号： G01N1/312 , B01J19/0046 , B01J2219/00722 , B01L3/508 , B01L7/52 , B01L9/52 , B01L2200/0684 , B01L2200/0689 , B01L2300/041 , B01L2300/0822 , B01L2300/1827 , B01L2300/185 , C12Q1/6841 , C12Q1/686 , C40B40/06 , Y10S435/809 , C12Q2543/101

28. 发明公开

EP0868530A1 A CASCADE NUCLEIC ACID AMPLIFICATION REACTION 失效
标题翻译：在KASKADEN VELAUFENDEVERVIELFÄLTIGUNGSREAKTIONVONNUKLEINSÄUREN
公开(公告)号：EP0868530A1
公开(公告)日：1998-10-07
申请号：EP96939821.0
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12N15 , C12Q1
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131
摘要： A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide. This reaction can be used in a process for the amplification of a particular DNA sequence wherein said DNA sequence is either circularized or inserted into a circular oligonucleotide, and the resulting circular DNA is used as a template for an endless copying process.
摘要翻译：由寡核苷酸单元的多重串联重复组成的DNA模板是通过无限复制的包含至少一个拷贝的所述寡核苷酸单元的环状寡核苷酸产生的，该核酸聚合酶能够进行链排列并基本上不含5' -3'核酸外切酶活性，在必需的核苷三磷酸存在下，如果需要，可以与能够与寡核苷酸的一部分结合的引物。该反应可用于扩增特定DNA序列的方法，其中所述DNA序列被环化或插入到环状寡核苷酸中，所得到的环状DNA用作环状复制过程的模板。

29. 发明公开

EP0826066A1 METHODS AND REAGENTS FOR COMBINED PCR AMPLIFICATION AND HYBRIDIZATION PROBING ASSAY 失效
标题翻译：方法和试剂用于PCR扩增与杂交Assey的组合
公开(公告)号：EP0826066A1
公开(公告)日：1998-03-04
申请号：EP96911592.0
申请日：1996-04-05
申请人： THE PERKIN-ELMER CORPORATION
发明人： MAYRAND, Paul E.
IPC分类号： C12N15 , C07H21 , C12P19 , C12Q1 , G01N21 , G01N33
CPC分类号： C12Q1/6818 , C07H21/04 , C12Q1/6853 , C12Q1/686 , Y10S435/81 , Y10S435/911 , C12Q2565/107 , C12Q2565/1015 , C12Q2525/186 , C12Q2525/125 , C12Q2543/101 , C12Q2537/101 , C12Q2563/113 , C12Q2531/113 , C12Q2527/101 , C12Q2521/513
摘要： An oligonucleotide probe is disclosed, the probe including an oligonucleotide, a fluorescer molecule attached to a first end of the oligonucleotide and a quencher molecule attached to the opposite end of the oligonucleotide. The probe is rendered impervious to digestion by the 5'→3' exonuclease activity of a polymerase and the 5'→3' extension by a polymerase. The invention also includes methods for performing combined PCR amplification and hybridization probing, one such method including the steps of contacting a target nucleic acid sequence with PCR reagents and an oligonucleotide probe as described above, and subjecting these reagents to thermal cycling. One preferred refinement of the above method further includes the addition of a strand displacer to facilitate amplification. Additional similar combined PCR hybridization methods are disclosed, such methods not requiring probes having their 5' ends protected, wherein (i) the polymerase lacks 5'→3' exonuclease activity, (ii) a 5'→3' exonuclease inhibitor is included, and (iii) an exonuclease deactivation step is performed.

30. 发明公开

EP3373822A1 IN VIVO COLLECTION AND LOCALIZED QUANTIFICATION AND PROFILING OF CIRCULATING CELLS, PROTEINS AND NUCLEIC ACIDS 无效
公开(公告)号：EP3373822A1
公开(公告)日：2018-09-19
申请号：EP16795000.5
申请日：2016-11-09
申请人： Medizinische Universität Graz
发明人： AHLFORD, Annika , EL-HELIEBI, Amin , BERNITZ, Mats Nilsson
IPC分类号： A61B10/00 , C12Q1/68
CPC分类号： C12Q1/6844 , A61B10/0045 , C12Q2525/307 , C12Q2531/125 , C12Q2537/143 , C12Q2543/101
摘要： The present invention relates to localized multiplex detection of RNA or proteins in situ within circulating cells that are captured in vivo by a collection device, or detection of free circulating nucleic acids captured by the same device. The device comprises a functional, three-dimensional capture surface equipped with detection receptors and the molecular assays are based on highly specific padlock probe ligation or proximity ligation assay (PLA) followed by rolling circle amplification (RCA).

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