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21. 发明授权

EP2817418B1 LABELING AND SAMPLE PREPARATION FOR SEQUENCING 有权转让
标题翻译：标记和样品制备用于测序
公开(公告)号：EP2817418B1
公开(公告)日：2017-10-11
申请号：EP13751450.1
申请日：2013-02-22
申请人： Raindance Technologies, Inc.
发明人： WATSON, Andrew
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N1/30 , C12Q1/6806 , C12Q1/6869 , C12Q2531/113 , C12Q2563/131 , C12Q2563/159 , C12Q2563/179 , C12Q2525/191 , C12Q2535/122 , C12Q2537/159 , C12Q2563/149 , C12Q2565/629
摘要： The invention provides methods for sequencing and sample preparation for sequencing and amplification.

22. 发明公开

EP3225698A1 CLOSED NUCLEIC ACID STRUCTURES 有权
标题翻译：封闭的核酸结构
公开(公告)号：EP3225698A1
公开(公告)日：2017-10-04
申请号：EP17160148.7
申请日：2012-09-06
申请人： Gen-Probe Incorporated
发明人： NELSON, Norman C , CHELLISERRY, Jijumon , BRENTANO, Steven T , LYAKHOV, Dmitry , FRIEDENBERG, Matthew , SHAPIRO, Anne-Laure
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6855 , C12Q1/6869 , C12Q2521/501 , C12Q2525/155 , C12Q2525/161 , C12Q2525/191 , C12Q2525/301 , C12Q2525/307 , C12Q2533/101
摘要： There is described a method of forming a closed nucleic acid structure comprising a segment of a target nucleic acid, the method comprising: (a) contacting a target nucleic acid with a primer pair under amplification conditions, each of the primers of the pair including a 5' phosphate group and a 3' segment, the 3' segments of the primers being target-binding segments; thereby forming an amplified nucleic acid comprising duplex target nucleic acid flanked by the primers of the pair duplexed with their complementary segments, wherein each strand of the amplified nucleic acid includes a 5' phosphate group and a 3' hydroxyl group; (b) denaturing the amplified nucleic acid and contacting a strand of the denatured amplified nucleic acid with a stem-loop adaptor having a 5' phosphate group, a 5' segment, a 3' segment having a stem-loop structure, and a 3' hydroxyl group, wherein the 5' segment is complementary to a segment at the 5' end of the amplified nucleic acid strand; (c) annealing the 5' segment of the stem-loop adaptor to the 5' end segment of the amplified nucleic acid strand, thereby forming a partially duplex, two-stranded intermediate structure wherein the 5' phosphate group of the amplified nucleic acid strand is separated by a nick from the 3' hydroxyl group of the stem-loop adaptor; and (d) providing a ligase that seals the nick between the 5' phosphate group of the amplified nucleic acid strand and the 3' hydroxyl group of the stem-loop adaptor and additionally links the 5' phosphate group of the stem-loop adaptor to the 3' hydroxyl group of the amplified nucleic acid strand, thereby forming a closed nucleic acid structure.
摘要翻译：描述了形成包含靶核酸片段的闭合核酸结构的方法，所述方法包括：（a）在扩增条件下使靶核酸与引物对接触，所述一对引物中的每一个均包含 5'磷酸基团和3'区段，引物的3'区段是靶标结合区段; 从而形成包含双链靶核酸的扩增核酸，所述双链靶核酸侧接与其互补链段复合的一对引物，其中扩增核酸的每条链包含5'磷酸基团和3'羟基; （b）使扩增的核酸变性，并使变性扩增核酸的链与具有5'磷酸基团，5'区段，具有茎环结构的3'区段和3'区段的茎环接头接触 '羟基，其中5'区段与扩增的核酸链5'末端的区段互补; （c）将茎环接头的5'区段退火至扩增的核酸链的5'端区段，由此形成部分双链体双链中间结构，其中扩增的核酸链的5'磷酸基团通过茎环适配器的3'羟基的缺口分开; 和（d）提供连接酶，其将扩增的核酸链的5'磷酸基团与茎环适配体的3'羟基之间的切口密封并且另外将茎环适配体的5'磷酸基团连接到扩增的核酸链的3'羟基，由此形成闭合的核酸结构。

23. 发明公开

EP3077545A4 METHODS FOR SEQUENCING NUCLEIC ACIDS 有权
标题翻译： VERFAHREN ZUR SEQUENZIERUNG VONNUKLEINSÄUREN
公开(公告)号：EP3077545A4
公开(公告)日：2017-09-27
申请号：EP14867494
申请日：2014-12-05
申请人： CENTRILLION TECH HOLDINGS CORP
发明人： ZHOU WEI , CRNOGORAC FILIP , MCGALL GLENN , CAO JIAN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6837 , C12Q2525/155 , C12Q2525/161 , C12Q2525/191 , C12Q2531/113 , C12Q2535/122 , C12Q2565/514 , C12Q2565/515 , C12Q2565/629
摘要： Provided herein are methods and compositions for the sequencing of long nucleic acids, such as DNA. The methods and compositions are suited for the spatial labeling and sequencing of long nucleic acid molecules.
摘要翻译：本文提供了用于测序长核酸如DNA的方法和组合物。该方法和组合物适合于长核酸分子的空间标记和测序。

24. 发明授权

EP2861787B1 COMPOSITIONS AND METHODS FOR NEGATIVE SELECTION OF NON-DESIRED NUCLEIC ACID SEQUENCES 有权
标题翻译：用于选择非期望的核酸序列的组合物和方法
公开(公告)号：EP2861787B1
公开(公告)日：2017-09-20
申请号：EP13806978.6
申请日：2013-03-15
申请人： Nugen Technologies, Inc.
发明人： ARMOUR, Christopher , AMORESE, Doug , LI, Bin , KURN, Nurith
IPC分类号： C12N15/10 , C12Q1/68 , C40B50/06
CPC分类号： C12Q1/6806 , C12N15/1093 , C12N15/1096 , C12Q1/6844 , C12Q2525/131 , C12Q2525/191 , C12Q2521/301 , C12Q2521/531 , C12Q2537/159

25. 发明公开

EP3114231A4 ENHANCED ADAPTOR LIGATION 有权转让
标题翻译： VERBESSERTE ADAPTERLIGATION
公开(公告)号：EP3114231A4
公开(公告)日：2017-09-13
申请号：EP15758489
申请日：2015-03-03
申请人： SWIFT BIOSCIENCES INC
发明人： MAKAROV VLADIMIR , LALIBERTE JULIE
IPC分类号： C12N15/66 , C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6855 , C12N9/1252 , C12N9/93 , C12Q1/6806 , C12Y207/07007 , C12Y605/01001 , C12Q2521/307 , C12Q2521/501 , C12Q2521/525 , C12Q2525/191
摘要： The present disclosure describes a method of adapter ligation to the ends of fragmented double-stranded DNA molecules.
摘要翻译：本公开描述了连接至片段化双链DNA分子末端的衔接子连接方法。

26. 发明公开

EP3207134A2 CONTIGUITY PRESERVING TRANSPOSITION 有权转让
标题翻译：对等保存转换
公开(公告)号：EP3207134A2
公开(公告)日：2017-08-23
申请号：EP15797490.8
申请日：2015-10-16
申请人： Illumina Cambridge Limited
发明人： STEEMERS, Frank J. , GUNDERSON, Kevin L. , ZHANG, Fan , BETLEY, Jason Richard , GORMLEY, Niall Anthony , MEULEMAN, Wouter , WEIR, Jacqueline , IOANNOU, Avgousta , JENKINS, Gareth , JACKSON, Rosamond , MORRELL, Natalie , POKHOLOK, Dmitry K. , NORBERG, Steven J. , HE, Molly , KIA, Amirali , GORYSHIN, Igor , PANTOJA, Rigo
IPC分类号： C12N15/10
CPC分类号： C12N15/1093 , C12N15/1065 , C12Q2525/191 , C12Q2563/179 , C12Q2565/514
摘要： Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.
摘要翻译：本文提供的实施方案涉及用于制备靶核酸的条形码化DNA片段的固定文库，鉴定基因组变体，确定靶核酸的邻接信息，定相信息和甲基化状态的方法和组合物。

27. 发明授权

EP2963127B1 High throughput detection of molecular markers based on restriction fragments 有权
标题翻译：基于限制性片段的分子标记的高通量检测
公开(公告)号：EP2963127B1
公开(公告)日：2017-08-16
申请号：EP15158621.1
申请日：2007-04-04
申请人： KEYGENE N.V.
发明人： Van Eijk, Michael Josephus Theresia , Hogers, René Cornelis Josephus
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6853 , C12Q2525/155 , C12Q2525/191 , C12Q2535/122 , C12Q2535/138
摘要： The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3' end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
摘要翻译：本发明涉及用于鉴定和检测分子标记的高通量方法，其中产生限制性片段并且连接包含（样品特异性）标识的合适衔接子。接头连接的限制性片段可以用在其3'末端携带选择性核苷酸的接头匹配引物选择性扩增。使用高通量测序方法对扩增的接头连接的限制性片段进行至少部分测序，并将限制性片段的序列部分与样品特异性标识符一起用作分子标记。

28. 发明公开

EP3192900A1 METHOD FOR CONSTRUCTING NUCLEIC ACID SINGLE-STRANDED CYCLIC LIBRARY AND REAGENTS THEREOF 有权转让
标题翻译：构建核酸单链循环库的方法和试剂
公开(公告)号：EP3192900A1
公开(公告)日：2017-07-19
申请号：EP14901597.6
申请日：2014-10-14
申请人： BGI Shenzhen Co., Limited
发明人： GENG, Chunyu , GUO, Rongrong , CHEN, Ruoying , HE, Lingyu , ZHANG, Wenwei , JIANG, Hui
IPC分类号： C40B50/06 , C12Q1/68
CPC分类号： C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/06 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
摘要： Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagents thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5' end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.
摘要翻译：本发明提供了构建核酸单链环状文库的方法及其试剂。该方法包括以下步骤：使用转座酶包埋复合物来随机破坏核酸并连接第一接头; 在间隙处连接第二连接体; 进行第一次PCR反应，其中一个引物的5'端具有第一亲和标签，产生两端连接于不同接头序列的产物; 将产物与具有第二亲和标签的固体载体结合; 退化并分离没有亲和标签的单链; 并环化单链。

29. 发明公开

EP3191630A4 METHOD FOR CONSTRUCTING SEQUENCING LIBRARY BASED ON BLOOD SAMPLE AND USAGE THEREOF IN DETERMINING FETAL GENETIC ABNORMALITY 有权
标题翻译： VERFAHREN ZUM AUFBAU EINER SEQUENZIERUNGSBIBLIOTHEK BASIEREND AUF EINER BLUTPROBE UND VERWENDUNG DAVON BEI DER BESTIMMUNG EINERFÖTALENGENETISCHENANOMALITÄT
公开(公告)号：EP3191630A4
公开(公告)日：2017-07-19
申请号：EP14901739
申请日：2014-09-26
申请人： BGI GENOMICS CO LTD
发明人： ZHANG YANYAN , CHEN FANG , JIANG HUI , GUO YULAI , ZENG PENG , XU XUN , YANG HUANMING , BALLINGER DENNIS G , BASHKIROV VLADIMIR I , SETHI HIMANSHU
IPC分类号： C40B50/06 , C12N15/10 , C12Q1/68 , C40B40/08
CPC分类号： C12N15/10 , B01J19/0046 , B01J2219/00529 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6853 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/06 , C40B40/08 , C40B50/06 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
摘要： Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagents thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5' end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.
摘要翻译：本发明提供了构建核酸单链环状文库的方法及其试剂。该方法包括以下步骤：使用转座酶包埋复合物来随机破坏核酸并连接第一接头; 在间隙处连接第二连接体; 进行第一次PCR反应，其中一个引物的5'端具有第一亲和标签，产生两端连接于不同接头序列的产物; 将产物与具有第二亲和标签的固体载体结合; 退化并分离没有亲和标签的单链; 并环化单链。

30. 发明公开

EP3191630A1 METHOD FOR CONSTRUCTING SEQUENCING LIBRARY BASED ON BLOOD SAMPLE AND USAGE THEREOF IN DETERMINING FETAL GENETIC ABNORMALITY 有权
标题翻译：构建基于血样的序列库的方法及其在确定胎儿遗传异常中的用途
公开(公告)号：EP3191630A1
公开(公告)日：2017-07-19
申请号：EP14901739.4
申请日：2014-09-26
申请人： BGI Genomics Co., Limited
发明人： ZHANG, Yanyan , CHEN, Fang , JIANG, Hui , GUO, Yulai , ZENG, Peng , XU, Xun , YANG, Huanming , BALLINGER, Dennis G. , BASHKIROV, Vladimir I. , SETHI, Himanshu
IPC分类号： C40B50/06 , C12Q1/68 , C12N15/10 , C40B40/08
CPC分类号： C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/06 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
摘要： Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagents thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5' end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.
摘要翻译：本发明提供了构建核酸单链环状文库的方法及其试剂。该方法包括以下步骤：使用转座酶包埋复合物来随机破坏核酸并连接第一接头; 在间隙处连接第二连接体; 进行第一次PCR反应，其中一个引物的5'端具有第一亲和标签，产生两端连接于不同接头序列的产物; 将产物与具有第二亲和标签的固体载体结合; 退化并分离没有亲和标签的单链; 并环化单链。

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