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21. 发明授权

EP1243661B1 The use of certain substances for improving heat stability of RNA 失效
标题翻译：使用某些物质，以提高RNA的热稳定性
公开(公告)号：EP1243661B1
公开(公告)日：2007-12-05
申请号：EP02013871.5
申请日：1997-07-24
申请人： The Institute of Physical and Chemical Research , Kabushiki Kaisha Dnaform
发明人： Hayashizaki, Yoshihide
IPC分类号： C12Q1/68 , C12N15/10 , C07H21/02 , C12N9/96
CPC分类号： C12N15/1096 , C12Q1/6844 , C12Q2527/101 , C12Q2521/107 , C12Q2527/125

22. 发明授权

EP0821058B1 Method for enhancing enzyme activity at elevated temperature 失效
标题翻译：在高温下增强酶活性的方法
公开(公告)号：EP0821058B1
公开(公告)日：2006-11-08
申请号：EP97112671.9
申请日：1997-07-24
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH , Kabushiki Kaisha Dnaform
发明人： Hayashizaki, Yoshihide
IPC分类号： C12N9/96 , C12N9/12 , C12Q1/68
CPC分类号： C12N9/1276 , C12N9/1252 , C12N9/22 , C12N9/96

23. 发明公开

EP3564679A1 ANALYSIS DEVICE 审中-公开
公开(公告)号：EP3564679A1
公开(公告)日：2019-11-06
申请号：EP17889505.8
申请日：2017-12-22
申请人： Kabushiki Kaisha DNAFORM
发明人： TSUJIMARU Koichiro
IPC分类号： G01N35/00 , C12M1/00 , C12Q1/68
摘要： The present invention provides a new smaller analysis device that can heat an analysis chip. The analysis device includes a set section in which an analysis chip is to be set; a housing chamber that accommodates the set section; a heating section that heats the analysis chip set in the set section; and an analysis section that analyzes the analysis chip set in the set section, wherein the housing chamber serves as an analysis chamber of the analysis chip set in the set section in the state where the set section is accommodated in the housing chamber, the analysis section is disposed at a position where the analysis chip set in the set section is analyzed in the state where the set section is accommodated in the housing chamber, and the heating section is a heating plate and is disposed at a position where the analysis chip set in the set section is heated in the state where the set section is accommodated in the housing chamber.

24. 发明公开

EP3401407A1 NUCLEIC ACID PROBE, METHOD FOR DESIGNING NUCLEIC ACID PROBE, AND METHOD FOR DETECTING TARGET SEQUENCE 有权
公开(公告)号：EP3401407A1
公开(公告)日：2018-11-14
申请号：EP18181035.9
申请日：2013-07-12
申请人： Kabushiki Kaisha Dnaform
发明人： Hayashizaki, Yoshihide , Hanami, Takeshi , Soma, Takahiro , Kimura, Yasumasa , Kanamori, Hajime , Mitani, Yasumasa
IPC分类号： C12Q1/68
摘要： The present invention provides method for detecting a target sequence in a nucleic acid molecule using a nucleic acid probe that hybridises to the target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule. The probe is immobilized on a solid phase.

25. 发明授权

EP2574669B1 BIOLOGICAL SAMPLE PRETREATMENT METHOD, METHOD FOR DETECTING RNA, AND PRETREATMENT KIT 有权
公开(公告)号：EP2574669B1
公开(公告)日：2018-10-17
申请号：EP12801801.7
申请日：2012-06-28
申请人： Kabushiki Kaisha Dnaform
发明人： HAYASHIZAKI Yoshihide , USUI Kengo , GODA Saori , NOMURA Kazuhito , KAWAI Yuki
IPC分类号： C12N15/09 , C07K14/79 , C12N9/36 , C12N9/99 , C12Q1/68
CPC分类号： G01N1/34 , C12N15/1003 , C12Q1/6806
摘要： The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.

26. 发明公开

EP3327444A1 KIT FOR ANALYSIS AND ANAYSIS METHOD USING SAME 审中-公开
公开(公告)号：EP3327444A1
公开(公告)日：2018-05-30
申请号：EP16830119.0
申请日：2016-04-27
申请人： Kabushiki Kaisha DNAFORM
发明人： TSUJIMARU Koichiro
IPC分类号： G01N35/08 , C12M1/00 , C12M1/34 , C12Q1/68 , G01N21/78 , G01N33/50 , G01N33/58 , G01N37/00 , C12N15/09
CPC分类号： C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/50 , G01N33/58 , G01N35/08 , G01N37/00
摘要： The present invention provides a miniaturizable tool that can analyze a target in a sample by a simple operation and an analysis method using the tool. An analysis chip is a syringe-type chip including: a syringe main body having an openable and closable tip; and a piston to be inserted into the syringe main body, the piston being a hollow body having a translucent closed-end at a side to be inserted into the syringe main body and an open-end at the other side. Analysis device includes a main body case that is a housing, the housing including: an insertion opening into which the syringe-type chip is to be inserted; and a void in communication with the insertion opening, wherein an opposite end of the insertion opening in an axial direction is a bottom; a heating unit that heats the syringe-type chip; a light source unit that emits light to the syringe-type chip; and a detection unit that is a columnar body that can be inserted into the hollow body of the piston and disposed at the bottom of the housing in the axial direction. In use, the syringe-type chip is inserted into the analysis device from a side of the piston and the detection unit of the analysis device is inserted into the piston of the syringe-type chip.

27. 发明公开

EP3144380A1 ANALYSIS DEVICE, ANALYSIS CHIP, ANALYSIS KIT, AND ANALYSIS METHOD USING SAME 审中-公开
标题翻译： ANALYSEVORRICHTUNG，ANALYSECHIP，ANALYSEKIT UND ANALYSEVERFAHREN DAMIT
公开(公告)号：EP3144380A1
公开(公告)日：2017-03-22
申请号：EP16743489.3
申请日：2016-01-28
申请人： Kabushiki Kaisha Dnaform
发明人： TSUJIMARU Koichiro
IPC分类号： C12M1/34 , C12M1/00 , C12Q1/68 , G01N35/08 , G01N37/00
CPC分类号： B01L7/52 , B01L2300/0654 , B01L2300/0663 , B01L2300/168 , B01L2400/0478 , C12M1/00 , C12M1/34 , C12Q1/68 , G01N35/08 , G01N37/00
摘要： The present invention provides a tool that can analyze a target in a sample by a simple operation and can be downsized and an analysis method using the tool. By inserting an analysis chip into an analysis device, a reaction of a sample and a reagent is analyzed. The analysis chip includes a parallel flow channel in which plural reaction flow channels are connected in parallel, and the axial direction of the parallel flow channel is a direction of inserting the analysis chip into the analysis device. The analysis device includes a main body case being a housing, including an insertion opening into which the analysis chip is to be inserted; and a void; a heating unit that causes a sample to thermally react with a reagent, the heating unit being disposed in the void at at least one of inner surfaces so as to face a parallel surface of the parallel flow channel of the analysis chip; a light source unit that emits light to the analysis chip, the light source unit being disposed in the void at least above or below a reaction flow channel located at at least one end of the parallel flow channel of the analysis chip and extended along the axial direction of the parallel flow channel; and a plurality of detection units that detect the thermal reactions, each detection unit being disposed in the void at a downstream side end in the insertion direction of the analysis chip so as to correspond to each reaction flow channel of the analysis chip.
摘要翻译：本发明提供了一种能够通过简单的操作分析样本中的目标并且可以使用该工具进行小型化和分析方法的工具。通过将分析芯片插入到分析装置中，分析样品和试剂的反应。分析芯片包括平行流动通道，其中多个反应流动通道并联连接，并行流动通道的轴向方向是将分析芯片插入分析装置的方向。分析装置包括：主体壳体，其是包括插入孔的壳体，分析芯片将插入其中; 和一个空白; 加热单元，其使样品与试剂发生热反应，所述加热单元设置在所述空隙中的至少一个内表面，以便面对所述分析芯片的平行流动通道的平行表面; 光源单元，其向所述分析芯片发光，所述光源单元设置在所述空隙中，所述光源单元至少位于位于所述分析芯片的平行流动通道的至少一端的反应流动通道的上方或下方，并沿着所述轴向延伸平行流通道方向; 以及多个检测单元，其检测热反应，每个检测单元设置在分析芯片的插入方向的下游侧的空隙中，以对应于分析芯片的每个反应流动通道。

28. 发明公开

EP3124622A1 FLUORESCENT LABELED SINGLE-STRANDED NUCLEIC ACID AND USE THEREOF 有权转让
标题翻译：维多利亚·马尔吉斯特EINZELSTRÄNGIGENUKLEINSÄUREUND VERWENDUNG DAVON
公开(公告)号：EP3124622A1
公开(公告)日：2017-02-01
申请号：EP15773110.0
申请日：2015-03-27
申请人： Riken , Kabushiki Kaisha DNAFORM
发明人： HANAMI Takeshi , HAYASHIZAKI Yoshihide , SOMA Takahiro , KIMURA Yasumasa
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/542
摘要： The present invention is intended to provide a novel fluorescent labeled single-stranded nucleic acid, by which the background of an exciton oligomer can be further reduced and the novel use thereof. The present invention relates to a labeled single-stranded nucleic acid having at least two fluorescent atomic group pairs that exhibit an exciton effect. The labeled single-stranded nucleic acid is characterized in that the emission peak wavelength of one of the fluorescent atomic group pairs (fluorescent atomic group pair A) is shorter than the excitation peak wavelength of the other fluorescent atomic group pair (fluorescent atomic group pair B), and the fluorescent atomic group pairs A and B have a Forster resonance energy transfer (FRET) effect. This fluorescent labeled single-stranded nucleic acid is usable as a primer for amplifying a target nucleic acid or a probe to be hybridized with a target nucleic acid.
摘要翻译：本发明旨在提供一种新的荧光标记单链核酸，通过其可以进一步降低激子低聚物的背景及其新用途。本发明涉及具有表现出激子效应的至少两个荧光原子团对的标记单链核酸。标记的单链核酸的特征在于荧光原子群对（荧光原子群对A）中的一个的发光峰值波长比另一个荧光原子群对（荧光原子群对B ），并且荧光原子群对A和B具有福斯特共振能量转移（FRET）效应。该荧光标记的单链核酸可用作扩增靶核酸或与靶核酸杂交的探针的引物。

29. 发明公开

EP2415878A1 Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same 审中-公开
标题翻译：一种用于核酸和用于检测的方法的扩增方法的突变核酸，使得
公开(公告)号：EP2415878A1
公开(公告)日：2012-02-08
申请号：EP11169071.5
申请日：2004-12-24
申请人： RIKEN , Kabushiki Kaisha Dnaform
发明人： Mitani, Yasumasa , Oka, Takanori , Hayashizaki, Yoshihide , Hayashi, Toshizo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q2537/163 , C12Q2531/113 , C12Q2521/514 , C12Q2525/301 , C12Q2525/161 , C12Q2537/125 , C12Q2522/101
摘要： A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3' end portion, a sequence (Ac') that hybridizes to a sequence (A) located in the 3' end portion of the target nucleic acid sequence. The first primer also contains, on the 5' side of the sequence (Ac'), a sequence (B') that hybridizes to a complementary sequence (Be) to a sequence (B) that is present on the 5' side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3' end portion, a sequence (Cc') that hybridizes to a sequence (C) located in the 3' end portion of a complementary sequence to the target nucleic acid sequence. The second primer also contains, on the 5' side of the sequence (Cc'), a folded sequence (D-Dc') that contains, on the same strand, two nucleic acid sequences that hybridize to each other.
摘要翻译：一种引物组确实允许靶核酸可以特异且高效地扩增。所述引物组，本发明的包括至少两个引物确实允许目标核酸序列进行扩增。包括在所述引物组的第一引物包含在其3“端部分，一序列（AC”）没有杂交到位于靶核酸序列的3“端部分序列（A）。因此，第一引物含有，对“（，序列（B”）的5序列AC）的侧“做杂交的互补序列（BE）的序列（B）那样存在于5”侧相对于在靶核酸序列的序列（A）。包括在所述引物组的第二引物含有，在其3“端部分，一序列（CC”）没有杂交到位于互补序列与靶核酸序列的3“端部分的序列（C）。因此，第二引物含有，对“（5序列CC）的侧”，折叠序列（D-DC“）确实包含，在相同的海滩，两个核酸序列杂交并海誓山盟。

30. 发明授权

EP1006180B1 Method for producing double stranded DNA whose terminal homopolymer part is eliminated and method for determining nucleotide sequence 有权
标题翻译：用于制备双链DNA，其终端部均聚物被消除，和方法，用于核苷酸序列的测定方法
公开(公告)号：EP1006180B1
公开(公告)日：2008-06-25
申请号：EP99122104.5
申请日：1999-11-05
申请人： RIKEN , Kabushiki Kaisha Dnaform
发明人： Hayashizaki, Yoshihide
IPC分类号： C12N15/10
CPC分类号： C12N15/1096 , Y10T436/143333

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