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    • 20. 发明公开
    • THREE-DIMENSIONAL INTERFEROMETRIC MICROSCOPY
    • 三维干涉显微镜
    • EP2988115A2
    • 2016-02-24
    • EP15183770.5
    • 2010-07-16
    • Howard Hughes Medical Institute
    • HESS, Harald F.
    • G01N21/64G02B21/22
    • G01N21/6458G01B9/02097G01B9/04G02B21/18G02B27/14
    • A statistically sparse subset of switchable optical sources in a sample (S) is activated, and the activated switchable optical sources are excited such that optical beams are emitted from the activated switchable optical sources along at least two optical paths (132, 142). A first wavefront modification in a first optical beam emitted from the activated switchable optical sources along a first optical path is introduced and a second wavefront modification in a second optical beam emitted from the activated switchable optical sources along a second optical path is introduced, the second wavefront modification being distinct from the first wavefront modification. The first and second optical beams are interfered with each other to produce a plurality of output beams (152, 154) and three-dimensional position information of the optical sources is determined based on an intensity of each output beam from the plurality of output beams.
    • 样本(S)中的可切换光源的统计上稀疏的子集被激活,并且被激活的可切换光源被激励,使得光束沿着至少两个光路(132,142)从激活的可切换光源发射。 引入沿着第一光路从激活的可切换光源发射的第一光束中的第一波前修正,并且引入沿着第二光路从激活的可切换光源发射的第二光束中的第二波前修正,第二 波前修改不同于第一波前修改。 第一和第二光束相互干涉以产生多个输出光束(152,154),并且基于来自多个输出光束的每个输出光束的强度来确定光源的三维位置信息。