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11. 发明公开

EP2873731A1 NUCLEIC ACID PROBE, METHOD FOR DESIGNING NUCLEIC ACID PROBE, AND METHOD FOR DETECTING TARGET SEQUENCE 有权
标题翻译： NUCLEIC探头，方法对于核酸探针用于检测的靶序列的设计和方法
公开(公告)号：EP2873731A1
公开(公告)日：2015-05-20
申请号：EP13820349.2
申请日：2013-07-12
申请人： Kabushiki Kaisha Dnaform
发明人： HAYASHIZAKI, Yoshihide , HANAMI, Takeshi , SOMA, Takahiro , KIMURA, Yasumasa , KANAMORI, Hajime , MITANI, Yasumasa
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6818 , C12N2330/30 , C12Q1/6827 , C12Q2525/117 , C12Q2535/131 , C12Q2537/101 , C12Q2563/107 , C12Q2565/1015 , C12Q2565/107
摘要： The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.

12. 发明授权

EP2873731B1 NUCLEIC ACID PROBE, METHOD FOR DESIGNING NUCLEIC ACID PROBE, AND METHOD FOR DETECTING TARGET SEQUENCE 有权
公开(公告)号：EP2873731B1
公开(公告)日：2018-08-29
申请号：EP13820349.2
申请日：2013-07-12
申请人： Kabushiki Kaisha Dnaform
发明人： HAYASHIZAKI, Yoshihide , HANAMI, Takeshi , SOMA, Takahiro , KIMURA, Yasumasa , KANAMORI, Hajime , MITANI, Yasumasa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C09B23/04 , C09B23/06 , C12N2330/30 , C12Q1/6827 , C12Q2525/117 , C12Q2535/131 , C12Q2537/101 , C12Q2563/107 , C12Q2565/1015 , C12Q2565/107
摘要： The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.

13. 发明授权

EP2746395B1 PRIMER SET, METHOD FOR AMPLIFYING TARGET NUCLEIC ACID SEQUENCE USING SAME, AND METHOD FOR DETECTING MUTATED NUCLEIC ACID USING SAME 有权
标题翻译：引物组，用于扩增目标核酸序列的方法以及使用该方法检测突变核酸的方法
公开(公告)号：EP2746395B1
公开(公告)日：2017-11-29
申请号：EP12829819.7
申请日：2012-09-07
申请人： Kabushiki Kaisha DNAFORM
发明人： HAYASHIZAKI, Yoshihide , KIMURA, Yasumasa , USUI, Kengo , TANAKA, Yuki , KAWAI, Yuki
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C07H21/04 , C12Q1/6844 , C12Q1/6853 , C12Q2525/155 , C12Q2525/301 , C12Q2531/119 , C12Q2531/125 , C12Q2525/161 , C12Q2527/101
摘要： The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3' side thereof, a sequence (A') that can hybridize to a sequence (A) on the 3' side of the target nucleic acid sequence. The second primer 1R includes, on the 3' side thereof, a sequence (B') that can hybridize to a sequence (B) on the 3' side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5' sides thereof, sequences (C) that are substantially identical to each other.

14. 发明公开

EP2894158A1 COMPOUND, NUCLEIC ACID, LABELING SUBSTANCE, AND DETECTION METHOD 有权
标题翻译： VERBINDUNG，NUKLEINSÄURE，MARKIERUNGSSUBSTANZ UND DETEKTIONSVERFAHREN
公开(公告)号：EP2894158A1
公开(公告)日：2015-07-15
申请号：EP13834990.7
申请日：2013-09-03
申请人： Kabushiki Kaisha DNAFORM
发明人： HAYASHIZAKI, Yoshihide , SOMA, Takahiro , HANAMI, Takeshi , KANAMORI, Hajime , BABA, Masaru
IPC分类号： C07H19/073 , C07H21/04
CPC分类号： C07H19/06 , C07H19/073 , C07H21/04 , C12Q1/6876 , Y02P20/55
摘要： The present invention provides a compound represented by the following chemical formula (I); a tautomer or stereoisomer of the compound; or a salt of the compound, the tautomer, or the stereoisomer.

In the chemical formula (I), R 1 and R 2 are each a Group 1 element or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R 1 and R 2 together may form a protecting group of an amino group. R 3 is a Group 1 element or a protecting group of a hydroxy group. R 4 is a Group 1 element or -PR 5 R 6 R 7 R 8 (R 5 , R 6 , R 7 , and R 8 are each a Group 1 element, a lone electron pair, a Group 16 element, a Group 17 element, or a protecting group of a phosphorus atom, and may be identical to or different from each other). J is a hydrogen atom or an arbitrary atomic group, A is a hydrogen atom, a hydroxy group, an alkyl group, an aralkyl group, an alkoxy group, an electron-withdrawing group, a silylene group, or a sulfide group, or alternatively, J and A together may form a linker. L is a single bond or a linker (a linking atom or a linking atomic group), the main chain length (the number of main chain atoms) of the linker is arbitrary, L may or may not contain each of C, N, O, S, P, and Si in the main chain, and may or may not contain each of a single bond, a double bond, a triple bond, an amide bond, an ester bond, a disulfide bond, an imino group, an ether bond, a thioether bond, and a thioester bond in the main chain. Z is an atomic group that can form a peptide bond with a labeling compound, or is an atom or atomic group including a label.
摘要翻译：本发明提供由下列化学式（I）表示的化合物。化合物的互变异构体或立体异构体; 或化合物的盐，互变异构体或立体异构体。在化学式（I）中，R 1和R 2各自为1族元素或氨基的保护基，并且可以相同或不同，或者R 1和R 2可以一起形成氨基保护基。 R 3是1族元素或羟基的保护基。 R 4是第1族元素或-PR 5 R 6 R 7 R 8（R 5，R 6，R 7和R 8各自是1族元素，单电子对，16族元素，第17族元素或磷原子的保护基，并且可以彼此相同或不同）。 J为氢原子或任意的原子团，A为氢原子，羟基，烷基，芳烷基，烷氧基，吸电子基，亚甲硅烷基或硫醚基，或者 J和A一起可形成连接体。 L是单键或连接体（连接原子或连接原子团），连接体的主链长度（主链原子数）是任意的，L可以含有也可以不含有C，N，O ，主链中的S，P和Si，并且可以包含或不包含单键，双键，三键，酰胺键，酯键，二硫键，亚氨基，醚键，硫醚键和主链中的硫酯键。 Z是可以与标记化合物形成肽键的原子团，或是包括标记的原子或原子团。

15. 发明公开

EP2746395A1 PRIMER SET, METHOD FOR AMPLIFYING TARGET NUCLEIC ACID SEQUENCE USING SAME, AND METHOD FOR DETECTING MUTATED NUCLEIC ACID USING SAME 有权
标题翻译：用于扩增的引物组的方法的核酸序列SO AND METHOD FOR DETECTION OF NUCLEIC突变SO
公开(公告)号：EP2746395A1
公开(公告)日：2014-06-25
申请号：EP12829819.7
申请日：2012-09-07
申请人： Kabushiki Kaisha DNAFORM
发明人： HAYASHIZAKI, Yoshihide , KIMURA, Yasumasa , USUI, Kengo , TANAKA, Yuki , KAWAI, Yuki
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C07H21/04 , C12Q1/6844 , C12Q1/6853 , C12Q2525/155 , C12Q2525/301 , C12Q2531/119 , C12Q2531/125 , C12Q2525/161 , C12Q2527/101
摘要： The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3' side thereof, a sequence (A') that can hybridize to a sequence (A) on the 3' side of the target nucleic acid sequence. The second primer 1R includes, on the 3' side thereof, a sequence (B') that can hybridize to a sequence (B) on the 3' side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5' sides thereof, sequences (C) that are substantially identical to each other.
摘要翻译：本发明提供的引物组包括底漆那样可以容易地设计，与在扩增距离可以缩短。提供了一种用于使用设置了一种用于等温扩增靶核酸序列4.引物组的引物包括：第一引物1F和第二引物1R。第一引物1F包括，在3“侧的序列，（A”）确实可以杂交到目标核酸序列的3“侧的序列（A）。第二引物1R包括，在3“侧的序列，（B”）没有可以杂交至在任的3“侧的序列（B）一个海滩从所述第一引物或靶核酸的互补海滩延伸酸序列4.第一引物1F和第二引物包括1R，在5“侧上，序列（C）做基本相同海誓山盟。

16. 发明授权

EP2894158B1 COMPOUND, NUCLEIC ACID, LABELING SUBSTANCE, AND DETECTION METHOD 有权
公开(公告)号：EP2894158B1
公开(公告)日：2018-06-20
申请号：EP13834990.7
申请日：2013-09-03
申请人： Kabushiki Kaisha DNAFORM
发明人： HAYASHIZAKI, Yoshihide , SOMA, Takahiro , HANAMI, Takeshi , KANAMORI, Hajime , BABA, Masaru
IPC分类号： C07H19/073 , C07H19/067 , C07H19/10 , C07H21/00 , C07H21/04
CPC分类号： C07H19/06 , C07H19/073 , C07H21/04 , C09B23/04 , C09B23/06 , C12Q1/6876 , Y02P20/55
摘要： The present invention provides a compound represented by the following chemical formula (I); a tautomer or stereoisomer of the compound; or a salt of the compound, the tautomer, or the stereoisomer. In the chemical formula (I), R 1 and R 2 are each a Group 1 element or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R 1 and R 2 together may form a protecting group of an amino group. R 3 is a Group 1 element or a protecting group of a hydroxy group. R 4 is a Group 1 element or -PR 5 R 6 R 7 R 8 (R 5 , R 6 , R 7 , and R 8 are each a Group 1 element, a lone electron pair, a Group 16 element, a Group 17 element, or a protecting group of a phosphorus atom, and may be identical to or different from each other). J is a hydrogen atom or an arbitrary atomic group, A is a hydrogen atom, a hydroxy group, an alkyl group, an aralkyl group, an alkoxy group, an electron-withdrawing group, a silylene group, or a sulfide group, or alternatively, J and A together may form a linker. L is a single bond or a linker (a linking atom or a linking atomic group), the main chain length (the number of main chain atoms) of the linker is arbitrary, L may or may not contain each of C, N, O, S, P, and Si in the main chain, and may or may not contain each of a single bond, a double bond, a triple bond, an amide bond, an ester bond, a disulfide bond, an imino group, an ether bond, a thioether bond, and a thioester bond in the main chain. Z is an atomic group that can form a peptide bond with a labeling compound, or is an atom or atomic group including a label.

17. 发明公开

EP1182458A1 METHOD FOR DETECTING PROTEINS UNDER MUTUAL INTERACTION 有权
标题翻译： VERFAHREN ZUM NACHWEIS VON PROTEINEN，DIE MITEINANDER INTERAGIEREN
公开(公告)号：EP1182458A1
公开(公告)日：2002-02-27
申请号：EP00921081.6
申请日：2000-04-28
申请人： Riken
发明人： HAYASHIZAKI, Yoshihide
IPC分类号： G01N33/566 , G01N33/68 , C07K17/00
CPC分类号： C40B30/04 , C07K1/13 , G01N33/6803 , G01N33/6842 , G01N33/6845 , Y10S436/824
摘要： A method for detecting proteins under mutual interaction which comprises mixing a protein having a label for detection synthesized by a cell-free protein synthesis method with another protein having a modification for separation, or synthesizing a protein having a label for detection and another protein having a modification for separation in a single system by the cell-free protein synthesis method; separating a pair of proteins formed by the interaction between these proteins with the use of the modification for separation as described above; and distinguishing these proteins with the use of the label for detection as described above. A screening method using this method.
摘要翻译：一种相互作用下蛋白质检测方法，其特征在于，将具有无细胞蛋白质合成方法合成检测标记的蛋白质与另一种具有分离修饰的蛋白质混合，或合成具有检测标记物的蛋白质和具有通过无细胞蛋白质合成方法在单一系统中进行分离修饰; 通过使用如上所述的分离修饰来分离由这些蛋白质之间的相互作用形成的一对蛋白质; 并使用如上所述的用于检测的标签区分这些蛋白质。使用该方法的筛选方法。

18. 发明公开

EP3394250A1 CELL REPROGRAMMING 审中-公开
公开(公告)号：EP3394250A1
公开(公告)日：2018-10-31
申请号：EP16877020.4
申请日：2016-12-23
申请人： Monash University , The University of Bristol , Riken , Cell Mogrify Limited
发明人： FIRAS, Jaber , POLO, Jose , GOUGH, Julian , HAYASHIZAKI, Yoshihide , RACKHAM, Owen
IPC分类号： C12N5/10 , C12N5/12 , C12N5/071 , C12N5/02 , C12N5/0735 , C12N5/0786 , C12N5/0783 , C12N5/0789 , C12N5/0775 , C12N5/077
CPC分类号： C12N5/0696 , C12N2501/60 , C12N2510/00
摘要： The invention relates to methods and compositions for converting one cell type to another cell type. Specifically, the invention relates to transdifferentiation of a cell to a different cell type. The invention relates to a method for determining the transcription factors required for conversion of a source cell to a cell exhibiting at least one characteristic of a target cell type. The invention also relates to method of reprogramming or forward programming a source cell.

19. 发明公开

EP1376120A1 METHOD OF ANALYZING POLYMER BY USING LASER ABRASION AND SYSTEM THEREFOR 审中-公开
标题翻译： VERFAHREN ZUR POLYMERANALYSE MIT LASERABRASION UND系统DAFÜR
公开(公告)号：EP1376120A1
公开(公告)日：2004-01-02
申请号：EP02700701.2
申请日：2002-02-22
申请人： Riken
发明人： HAYASHIZAKI, Yoshihide , TANIHATA, Isao, c/o RIKEN
IPC分类号： G01N27/64 , H01J49/10
CPC分类号： H01J49/164 , G01N33/442
摘要： It becomes possible to simultaneously atomize and ionize atoms constituting a polymer with the use of a single laser thereby highly simplifying the constitution of a system. A method of analyzing a polymer which comprises abrading the polymer to be analyzed by irradiating with laser beams to thereby atomize the polymer into the constituting elements thereof, then ionizing the elements and analyzing the constituting elements thus ionized. The laser beams with which the polymer to be analyzed is irradiated for the abrasion are ultrashort pulse laser beams. By irradiating the polymer with the ultrashort pulse laser beams to thereby abrade, the polymer can be atomized and ionized at the same time. Then the thus ionized constituting elements are analyzed.
摘要翻译：可以通过使用单个激光器同时雾化和离子化构成聚合物的原子，从而高度简化了系统的构成。一种分析聚合物的方法，其包括通过用激光束照射来研磨待分析的聚合物，从而将聚合物雾化成其组成元素，然后电离元件并分析这些电离的构成元素。要分析聚合物的激光束被照射用于磨损是超短脉冲激光束。通过用超短脉冲激光束照射聚合物从而磨损，可以同时对聚合物进行雾化和离子化。然后分析如此电离的构成元素。

20. 发明公开

EP1348035A2 METHOD FOR BASE SEQUENCING AND BIOLOGICALLY ACTIVE NUCLEIC ACIDS 审中-公开
标题翻译：方法碱基测序和生物活性NUCLEIC
公开(公告)号：EP1348035A2
公开(公告)日：2003-10-01
申请号：EP01998557.1
申请日：2001-11-28
申请人： Riken
发明人： HAYASHIZAKI, Yoshihide
IPC分类号： C12Q1/68 , C07H21/00 , C12N15/10 , C12N15/11 , C12P19/34 , G01N33/53 , C07H19/20 , C07H19/10 , C07B61/00
CPC分类号： C12N15/115 , C07H19/10 , C07H19/20 , C07H21/00 , C12N2310/33 , C12Q1/6869 , C40B30/04 , G01N33/6845 , C12Q2525/117
摘要： Aptamers are nucleic acids and similar molecules, such as peptide-nucleic acids, that specifically bind to a ligand such as a protein or peptide. The present invention provides aptamers comprising at least one base capable of base pairing and different from the standard Watson-Crick bases. The present invention also relates to a method for preparation of such aptamers and to methods for sequencing nucleic acids that comprise at least one base capable of base pairing and different from the standard Watson-Crick bases.

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