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11. 发明公开

EP4136964A1 PLANT BODY OF GENUS NICOTIANA WITH LOW ALKALOID CONTENT AND PRODUCTION METHOD THEREOF 审中-实审
公开(公告)号：EP4136964A1
公开(公告)日：2023-02-22
申请号：EP21789517.6
申请日：2021-04-19
申请人： Japan Tobacco Inc.
发明人： TAKEUCHI, Takanori , ARAI, Masao , UDAGAWA, Hisashi , MAGOME, Hiroshi , NISHIGUCHI, Ryo , TAKAKURA, Yoshimitsu
IPC分类号： A01H5/00 , C12N15/29 , A24B15/10 , A01H6/82
摘要： An embodiment of the present invention provides a tobacco plant having a low alkaloid content. In the tobacco plant, a function of a gene encoding aspartate oxidase AO2 is suppressed.

12. 发明授权

EP2042602B1 COSMID VECTOR FOR PLANT TRANSFORMATION AND USE THEREOF 有权转让
标题翻译：粘粒植物和使用变换THEREOF
公开(公告)号：EP2042602B1
公开(公告)日：2012-12-26
申请号：EP07767526.2
申请日：2007-06-25
申请人： Japan Tobacco, Inc.
发明人： TAKAKURA, Yoshimitsu , KOMARI, Toshihiko , ISHIDA, Yuji , KOMORI, Toshiyuki , HIEI, Yukoh , MINE, Toshiki , IMAYAMA, Teruyuki
IPC分类号： C12N15/82 , A01H5/00 , C12N1/21 , C12N5/10
CPC分类号： C12N15/82 , C12N15/8205

13. 发明公开

EP2493914A1 DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING DTP21 POLYPEPTIDES 审中-公开
标题翻译： DRY抗性植物及相关结构和方法的研究FOR DTP21编码基因
公开(公告)号：EP2493914A1
公开(公告)日：2012-09-05
申请号：EP10773517.7
申请日：2010-11-01
申请人： E. I. du Pont de Nemours and Company , Pioneer-Hi-Bred International, Inc. , Japan Tobacco Inc
发明人： KASHIHARA, Masakazu , KOMORI, Toshiyuki , OKA, Ichiro , USAMI, Satoru , KATO, Norio , HIEI, Yukoh , TAKAKURA, Yoshimitsu , KOMARI, Toshihiko , IMAYAMA, Teruyuki , TINGEY, Scott, V. , SAKAI, Hajime , ALBERTSEN, Marc, C. , LUCK, Stanley
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8273 , C07K14/415 , C12N15/821
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

14. 发明公开

EP2314681A1 MODIFIED BIOTIN-BINDING PROTEIN 有权
标题翻译：修饰生物素结合蛋白
公开(公告)号：EP2314681A1
公开(公告)日：2011-04-27
申请号：EP09806750.7
申请日：2009-08-13
申请人： Japan Tobacco, Inc.
发明人： TAKAKURA, Yoshimitsu , ICHIKAWA, Masako
IPC分类号： C12N15/09 , C07K14/375 , C07K17/00 , G01N33/531
CPC分类号： C07K14/375 , G01N33/531 , G01N2333/36
摘要： The present invention relates to a modified biotin-binding protein. The modified biotin-binding protein of the present invention includes an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one to several amino acid mutations in the sequence represented by SEQ ID NO: 2, or an amino acid sequence having 80% or more identity to the sequence represented by SEQ ID NO: 2, and having a biotin-binding activity, wherein at least one residue selected from the group consisting of:
1) an arginine residue at position 104 of SEQ ID NO: 2;
2) a lysine residue at position 141 of SEQ ID NO: 2;
3) a lysine residue at position 26 of SEQ ID NO: 2; and
4) a lysine residue at position 73 of SEQ ID NO: 2

is replaced with an acidic amino acid residue or a neutral amino acid residue.
摘要翻译：本发明涉及修饰的生物素结合蛋白。本发明的修饰的生物素结合蛋白包括SEQ ID NO：2所示的氨基酸序列，在SEQ ID NO：2所示的序列中具有1〜数个氨基酸突变的氨基酸序列，或氨基酸序列与SEQ ID NO：2表示的序列具有80％以上的同一性，并且具有生物素结合活性，其中至少一种选自以下的残基：1）SEQ ID NO：2的104位的精氨酸残基， 2; 2）SEQ ID NO：2的141位的赖氨酸残基; 3）SEQ ID NO：2的26位的赖氨酸残基; 和4）SEQ ID NO：2的第73位的赖氨酸残基被酸性氨基酸残基或中性氨基酸残基取代。

15. 发明公开

EP2112168A1 USE OF THERMOSTABLE BIOTIN-BINDING PROTEIN AND SOLID SUPPORT HAVING THE PROTEIN BOUND THERETO 有权
标题翻译：维生素EENES THERMOSTABILEN BIOTINBINDENDEN蛋白质
公开(公告)号：EP2112168A1
公开(公告)日：2009-10-28
申请号：EP07860501.1
申请日：2007-12-28
申请人： Japan Tobacco Inc.
发明人： TAKAKURA, Yoshimitsu , USAMI, Satoru , ICHIKAWA, Masako
IPC分类号： C07K17/00 , C07K14/375 , C07K16/14 , C12N15/09 , C12P21/02 , C12Q1/68 , G01N33/53
CPC分类号： C07K14/001 , C07K14/375 , C07K16/14 , G01N33/543 , G01N33/68 , G01N2333/36
摘要： The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70°C or more.
摘要翻译：本发明涉及具有附着于其上的耐热生物素结合蛋白的固体支持体。本发明还涉及具有附着于其上的耐热生物素结合蛋白的本发明的固体支持物的用途。本发明还涉及通过耐热生物素结合蛋白的生物素连接物质的纯化，浓缩，检测和/或捕获等技术领域。用于本发明的固体支持物中的这种生物素结合蛋白是耐热的，因此可用于涉及暴露于70℃或更高温度的测定系统中。

16. 发明公开

EP4140293A1 TOBACCO PLANT BODY AND METHOD FOR PRODUCING SAME 无效
公开(公告)号：EP4140293A1
公开(公告)日：2023-03-01
申请号：EP21791966.1
申请日：2021-04-21
申请人： Japan Tobacco Inc.
发明人： MAGOME, Hiroshi , ARAI, Masao , OYAMA, Kiyoshi , TAKAKURA, Yoshimitsu , NISHIGUCHI, Ryo
IPC分类号： A01H5/00 , A01H6/82 , A24B13/00 , A24B15/00 , C12N15/10 , C12N15/113 , C12N15/29
摘要： The object of an embodiment of the present invention is to improve the quality of a tobacco product. The present invention relates to a tobacco plant in which a mutation is introduced in a genome, the mutation causing suppression of a function of a specific endogenous gene.

17. 发明授权

EP2447364B1 MODIFIED BIOTIN-CONJUGATED PROTEIN 有权
标题翻译：修饰型生物素结合蛋白质
公开(公告)号：EP2447364B1
公开(公告)日：2017-02-01
申请号：EP09846508.1
申请日：2009-06-24
申请人： Japan Tobacco, Inc.
发明人： TAKAKURA, Yoshimitsu , TSUNASHIMA, Masako , SOFUKU, Kozue
IPC分类号： C12N15/09 , C07K14/37 , C07K17/00 , C07K14/375
CPC分类号： C07K14/375 , C07K2319/22 , G01N2333/36

18. 发明授权

EP2314681B1 MODIFIED BIOTIN-BINDING PROTEIN 有权
标题翻译：修饰型生物素结合蛋白
公开(公告)号：EP2314681B1
公开(公告)日：2015-04-22
申请号：EP09806750.7
申请日：2009-08-13
申请人： JAPAN TOBACCO INC.
发明人： TAKAKURA, Yoshimitsu , ICHIKAWA, Masako
IPC分类号： C12N15/09 , C07K14/375 , C07K17/00 , G01N33/531
CPC分类号： C07K14/375 , G01N33/531 , G01N2333/36

19. 发明授权

EP2112168B1 USE OF THERMOSTABLE BIOTIN-BINDING PROTEIN 有权
标题翻译：使用热稳定的生物素结合蛋白
公开(公告)号：EP2112168B1
公开(公告)日：2014-10-01
申请号：EP07860501.1
申请日：2007-12-28
申请人： Japan Tobacco Inc.
发明人： TAKAKURA, Yoshimitsu , USAMI, Satoru , ICHIKAWA, Masako
IPC分类号： G01N33/543 , G01N33/68 , C07K14/00 , C07K16/14 , C07K14/375
CPC分类号： C07K14/001 , C07K14/375 , C07K16/14 , G01N33/543 , G01N33/68 , G01N2333/36

20. 发明公开

EP2660320A1 MODIFIED TAMAVIDIN 有权
标题翻译：改性T ID啶
公开(公告)号：EP2660320A1
公开(公告)日：2013-11-06
申请号：EP11853702.6
申请日：2011-12-28
申请人： Japan Tobacco, Inc.
发明人： TAKAKURA, Yoshimitsu , OKA, Naomi
IPC分类号： C12N15/00 , C07K1/14 , C07K14/375 , C07K17/00 , C12N15/09 , G01N33/53 , G01N33/68
CPC分类号： C07K14/37 , C07K14/375 , C07K14/465 , G01N33/53
摘要： The present invention provides modified tamabidin 2, which is a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or more amino acid mutations in the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having an identity of not less than 80% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine. The modified biotin-binding protein has remarkable heat resistance.
摘要翻译：本发明提供了修饰的脒苷2，其是包含SEQ ID NO：2所示的氨基酸序列的修饰的生物素结合蛋白，在SEQ ID NO：2的氨基酸序列中具有一个或多个氨基酸突变的氨基酸序列。 2或与SEQ ID NO：2的氨基酸序列具有不小于80％同一性且具有生物素结合活性的氨基酸序列，其中SEQ ID NO：2的位置115处的天冬酰胺残基被置换为半胱氨酸。修饰的生物素结合蛋白具有显着的耐热性。

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