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1. 发明公开

EP2447364A1 MODIFIED BIOTIN-CONJUGATED PROTEIN 有权
标题翻译：改良生物素康酮蛋白
公开(公告)号：EP2447364A1
公开(公告)日：2012-05-02
申请号：EP09846508.1
申请日：2009-06-24
申请人： Japan Tobacco, Inc.
发明人： TAKAKURA, Yoshimitsu , TSUNASHIMA, Masako , SOFUKU, Kozue
IPC分类号： C12N15/09 , C07K14/37 , C07K17/00
CPC分类号： C07K14/375 , C07K2319/22 , G01N2333/36
摘要： The present invention provides a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2 or its modified sequence and having a biotin-binding activity and replacement selected from the group consisting of:
1) replacement of the 36th serine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond;
2) replacement of the 80th tryptophan residue of SEQ ID NO: 2 with a hydrophilic amino acid residue;
3) replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond;
4) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond;
5) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid that does not form a hydrogen bond; and
6) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue, replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond, and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid that does not form a hydrogen bond.
摘要翻译：本发明提供一种修饰的生物素结合蛋白，其包含由SEQ ID NO：2或其修饰序列表示的氨基酸序列，并具有生物素结合活性和选自以下的置换：1）替换第36位丝氨酸残基具有不形成氢键的氨基酸残基的SEQ ID NO：2; 2）用亲水氨基酸残基置换SEQ ID NO：2的第80个色氨酸残基; 3）用不形成氢键的氨基酸残基替换SEQ ID NO：2的第116位天冬氨酸残基; 4）用苏氨酸，丝氨酸或酪氨酸残基替换SEQ ID NO：2的第46个脯氨酸残基，并用不形成氢键的氨基酸残基替换SEQ ID NO：2的第78个苏氨酸残基; 5）用苏氨酸，丝氨酸或酪氨酸残基替换SEQ ID NO：2的第46个脯氨酸残基，用不形成氢键的氨基酸替换SEQ ID NO：2的第116位天冬氨酸残基; 和6）用苏氨酸，丝氨酸或酪氨酸残基替换SEQ ID NO：2的第46个脯氨酸残基，用不形成氢键的氨基酸残基替代SEQ ID NO：2的第78个苏氨酸残基，并用不形成氢键的氨基酸替代SEQ ID NO：2的第116位天冬氨酸残基。

2. 发明公开

EP2006378A1 NOVEL BETA-GALACTOSIDE ALPHA- 2,6-SIALYLTRANSFERASE, GENE CODING FOR THE TRANSFERASE AND PROCESS FOR PRODUCING THE SAME 审中-公开
标题翻译： NEWβ-半乳糖苷α-2,6唾液酸转移酶进行编码转移酶基因和方法及其制造
公开(公告)号：EP2006378A1
公开(公告)日：2008-12-24
申请号：EP06782636.2
申请日：2006-08-10
申请人： Japan Tobacco, Inc.
发明人： TSUKAMOTO, Hiroshi , MINE, Toshiki , TAKAKURA, Yoshimitsu , YAMAMOTO, Takeshi
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N1/21 , C12N9/10
CPC分类号： C12N9/1081 , C07H21/04
摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了一种新颖的²galactoside-±具有高的生产率和/或高活性，和核酸编码唾液酸转移酶的2,6-唾液酸转移酶。本发明因此提供了微生物生产唾液酸转移酶。本发明还提供携带核酸编码唾液酸转移酶的载体，并用该载体转化的宿主细胞，以及用于生产重组²galactoside-±2,6唾液酸转移酶的方法。

3. 发明公开

EP2416159A1 METHOD FOR DETECTING ANTIBODY AGAINST SITH-1 IN BIOLOGICAL SAMPLE 审中-公开
标题翻译：生物样品中检测抗SITH-1抗体的方法
公开(公告)号：EP2416159A1
公开(公告)日：2012-02-08
申请号：EP10758800.6
申请日：2010-03-31
申请人： Japan Tobacco, Inc. , Virus Ikagaku Kenkyusho Inc.
发明人： TAKAKURA, Yoshimitsu , OKA, Naomi , KONDO, Kazuhiro , KOBAYASHI, Nobuyuki
IPC分类号： G01N33/569 , G01N33/53 , C07K14/03
CPC分类号： C07K14/005 , C07K2319/70 , C12N2710/16522 , G01N33/56994 , G01N33/6854 , G01N2333/03 , G01N2469/20
摘要： The present invention provides a method for detecting an antibody against a small protein encoded by the intermediate stage transcript of HHV-6 (SITH-1) in a biological sample.
The method of the present invention comprises:
1) providing the SITH-1 protein;
2) binding the SITH-1 protein provided in step 1) to a carrier; and
3) contacting the biological sample with the SITH-1 protein-bound carrier prepared in step 2) to detect the SITH-1 protein antibody.
摘要翻译：本发明提供了检测生物样品中由HHV-6（SITH-1）的中间阶段转录物编码的小蛋白质的抗体的方法。本发明的方法包括：1）提供SITH-1蛋白质; 2）将步骤1）中提供的SITH-1蛋白与载体结合; 和3）将生物样品与步骤2）中制备的结合SITH-1蛋白的载体接触以检测SITH-1蛋白抗体。

4. 发明公开

EP2267151A1 VIRUS TITRATION METHOD 审中-公开
标题翻译：病毒滴定法
公开(公告)号：EP2267151A1
公开(公告)日：2010-12-29
申请号：EP09727611.7
申请日：2009-03-31
申请人： Japan Tobacco, Inc.
发明人： TAKAKURA, Yoshimitsu , ICHIKAWA, Masako , KONDO, Kazuhiro , SHIMIZU, Akihiro
IPC分类号： C12Q1/06 , C12Q1/68 , G01N33/569
CPC分类号： G01N33/56983 , G01N2333/03 , G01N2333/36
摘要： The present invention relates to a method of quantitatively determining the number of human herpesvirus (HHV) collected from a body fluid and a kit for performing the method. Conventionally, a trained technician has been required to accurately quantitatively determine a number of HHV collected from a body fluid. The method of the present invention is a novel method of quantitative determination that enables measurement of a number of HHV in a body fluid to be simply, accurately, and efficiently determined. The method of the present invention can enable continuous evaluation of the number of HHV in body fluids and, therefore, can be applied to quantitative evaluation of the accumulation of fatigue.
摘要翻译：本发明涉及定量测定从体液中收集的人疱疹病毒（HHV）的数量的方法和用于执行该方法的试剂盒。通常，要求训练有素的技术人员准确定量测定从体液中收集的一些HHV。本发明的方法是定量确定的新方法，其能够简单，准确且有效地确定体液中的HHV的数量的测量。本发明的方法可以对体液中HHV的数量进行连续评估，因此可以应用于定量评估疲劳累积。

5. 发明公开

EP2184356A1 METHOD OF BINDING PROTEIN TO SUPPORT USING TAMAVIDIN 审中-公开
标题翻译： VERFAHREN ZUR BINDEN EINES蛋白质EINENTRÄGERMIT TAMAVIDIN
公开(公告)号：EP2184356A1
公开(公告)日：2010-05-12
申请号：EP08828874.1
申请日：2008-08-28
申请人： Japan Tobacco Inc.
发明人： TAKAKURA, Yoshimitsu , ICHIKAWA, Masako , USAMI, Satoru , YAMAMOTO, Takeshi , TSUKAMOTO, Hiroshi , KAJIWARA, Hitomi , OKA, Naomi
IPC分类号： C12N15/09 , C07K17/00 , C07K19/00 , C07K14/375
CPC分类号： C07K17/06 , C07K14/375 , C07K2319/20 , G01N2333/36
摘要： The present invention provides a method of binding a protein to a carrier in such a way that the protein is not impaired in its function but can be allowed to act more efficiently than when it is bound directly.
The method of the present invention for binding a protein to a carrier comprises:
preparing a biotin-bound carrier;
preparing a fusion protein having the protein bound to a tamavidin; and
binding the protein to the carrier via tamavidin-biotin bonds.
摘要翻译：本发明提供了一种将蛋白质与载体结合的方法，使得蛋白质在其功能上不受损害，但可以比直接结合时更有效地起作用。将蛋白质与载体结合的本发明的方法包括：制备生物素结合的载体; 制备具有与蛋白糊精结合的蛋白质的融合蛋白; 并通过tamavidin-biotin键将蛋白质与载体结合。

6. 发明公开

EP3504963A1 VIRUS-RESISTANT TOBACCO AND BREEDING METHOD THEREFOR 审中-公开
公开(公告)号：EP3504963A1
公开(公告)日：2019-07-03
申请号：EP17843725.7
申请日：2017-08-25
申请人： JAPAN TOBACCO INC.
发明人： TAKAKURA, Yoshimitsu , SHINJO, Akira , UDAGAWA, Hisashi , KOGA, Kazuharu
IPC分类号： A01H5/00 , A01H1/00 , A24B1/00 , C12N15/09
摘要： In order to provide tobacco resistant to a virus, tobacco in accordance with the present invention is arranged such that: (i) a translation initiation factor eIF(iso)4E protein which is non-functional with respect to a virus is produced or (ii) expression of a translation initiation factor eIF(iso)4E gene is suppressed; and (a) a translation initiation factor eIF4E2 protein, which is non-functional with respect to a virus, is produced or (b) expression of a translation initiation factor eIF4E2 gene is suppressed.

7. 发明授权

EP2660320B1 MODIFIED TAMAVIDIN 有权
标题翻译：修饰的TAMAVIDIN
公开(公告)号：EP2660320B1
公开(公告)日：2017-09-27
申请号：EP11853702.6
申请日：2011-12-28
申请人： Japan Tobacco Inc.
发明人： TAKAKURA, Yoshimitsu , OKA, Naomi
IPC分类号： C12N15/00 , C07K1/14 , C07K14/375 , C07K17/00 , C12N15/09 , G01N33/53 , G01N33/68
CPC分类号： C07K14/37 , C07K14/375 , C07K14/465 , G01N33/53

8. 发明公开

EP2755029A1 METHOD FOR INHIBITING NON-SPECIFIC BINDING IN STEP OF DETECTING SUBSTANCE IN BIOLOGICAL SAMPLE, AND AGENT FOR USE IN THE METHOD 无效
标题翻译：一种抑制不特异性结合在步骤用于识别物质的生物样本和使用在这个过程中
公开(公告)号：EP2755029A1
公开(公告)日：2014-07-16
申请号：EP12820586.1
申请日：2012-08-01
申请人： Japan Tobacco, Inc.
发明人： TAKAKURA, Yoshimitsu , OKA, Naomi
IPC分类号： G01N33/543 , G01N33/53 , C07K14/37 , C12N15/09
CPC分类号： G01N33/54393 , C07K14/36 , C07K14/37 , C07K14/375 , C07K14/435 , C07K14/465 , G01N33/5306
摘要： The present invention provides a method of inhibiting non-specific binding in a step of detecting a substance in a biological sample. The invention provides a method of inhibiting non-specific binding in a step of detecting a substance in a biological sample, the method comprising:
bringing a biotin-binding protein monomer not substantially having biotin-binding ability into contact with the biological sample, wherein
in the step of detecting, a substance capable of specifically binding to the substance to be detected is immobilized to a carrier through binding between biotin and the biotin-binding protein before or after binding of the substance to be detected to the substance capable of specifically binding to the substance to be detected.
摘要翻译：本发明提供的抑制非特异性的检测生物样品中的物质的步骤结合的方法。本发明提供的抑制非特异性的检测生物样品中的物质的步骤的结合，该方法包括以下步骤：使生物素结合蛋白单体实质上不具有生物素结合能力与所述生物样品，worin在接触检测的步骤中，能够特异性地结合到待检测是通过之前或待检测到能够特异性结合到该物质的物质结合后的生物素和生物素结合蛋白之间的结合固定化到载体中的物质的物质待检测的物质。

9. 发明公开

EP2116598A1 NOVEL BETA-GALACTOSIDE-ALPHA-2,6-SIALYLTRANSFERASE, GENE ENCODING THE SAME AND METHOD FOR ENHANCING ENZYMATIC ACTIVITY 审中-公开
标题翻译：新的β-半乳糖苷α-2,6唾液酸转移酶编码基因体和法提高酶活性
公开(公告)号：EP2116598A1
公开(公告)日：2009-11-11
申请号：EP08721168.6
申请日：2008-03-03
申请人： Japan Tobacco Inc.
发明人： YAMAMOTO, Takeshi , TAKAKURA, Yoshimitsu , MINE, Toshiki , HAMADA, Yoko , KAJIWARA, Hitomi , ICHIKAWA, Masako , TSUKAMOTO, Hiroshi
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12P21/02
CPC分类号： C12N9/1081
摘要： The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β - galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了在非常有用的，新颖的²galactoside-±在中性到碱性范围具有在最佳反应pH 2,6-唾液酸转移酶，和编码核酸的唾液酸转移酶。本发明还提供携带核酸编码唾液酸转移酶的载体，并用该载体转化的宿主细胞，以及用于生产重组²的方法 - galactoside-±2,6-唾液酸转移酶。

10. 发明公开

EP4298897A1 PLANT BODY OF GENUS NICOTIANA AND PRODUCTION METHOD THEREFOR 无效
公开(公告)号：EP4298897A1
公开(公告)日：2024-01-03
申请号：EP22759514.7
申请日：2022-02-18
申请人： Japan Tobacco Inc.
发明人： TAKEUCHI, Takanori , UDAGAWA, Hisashi , TAKAKURA, Yoshimitsu , MAGOME, Hiroshi , NISHIGUCHI, Ryo
IPC分类号： A01H6/82 , A24B15/10 , C12N15/113 , C12N15/29
摘要： Provided are a tobacco plant in which a high solavetivone content can be achieved without inoculation with a virus, and a method for producing the tobacco plant. In a tobacco plant in accordance with an aspect of the present invention, a function of an endogenous CA1 gene is suppressed.

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