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91. 发明公开

EP3150722A1 PRODUCTION OF CLOSED LINEAR DNA 有权转让
标题翻译：制作肛门切除术线粒体DNA
公开(公告)号：EP3150722A1
公开(公告)日：2017-04-05
申请号：EP16195596.8
申请日：2010-02-01
申请人： Touchlight IP Limited
发明人： HILL, Vanessa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , A61K39/00 , A61K48/00 , A61K2039/53 , C12N9/0069 , C12N15/11 , C12Q1/6844 , C12Q1/6846 , C12Y113/12007 , C12Q2521/113 , C12Q2521/507 , C12Q2531/125 , C12Q2525/301
摘要： The invention relates to a process for in vitro cell-free production of closed linear DNA comprising amplifying DNA from a DNA template comprising more than one protelomerase target sequence, and contacting said amplified DNA with at least one protelomerase under conditions promoting production of closed linear DNA. The invention also relates to concatameric DNA comprising multiple repeats of a DNA template, said DNA template comprising at least one protelomerase target sequence.
摘要翻译：本发明涉及一种用于体外无细胞生产闭合线性DNA的方法，包括从包含多于一种端粒酶靶序列的DNA模板扩增DNA，并在促进闭合线性DNA生成条件下将所述扩增的DNA与至少一种蛋白端点酶接触。本发明还涉及包含DNA模板的多个重复序列的连续DNA，所述DNA模板包含至少一个端粒酶靶序列。

92. 发明公开

EP3132029A2 MODIFIED TRANSPOSASES FOR IMPROVED INSERTION SEQUENCE BIAS AND INCREASED DNA INPUT TOLERANCE 审中-公开
标题翻译：用于改进INSERTIONSSEQUENZBIAS改进的转座，增加耐受性DNA ENTER
公开(公告)号：EP3132029A2
公开(公告)日：2017-02-22
申请号：EP15719091.9
申请日：2015-04-15
申请人： Illumina, Inc.
发明人： GLOECKNER, Christian , KIA, Amirali , BOMATI, Erin , HE, Molly , GRUNENWALD, Haiying Li , KUERSTEN, Scott , OSOTHPRAROP, Trina Faye , HASKINS, Darin , BURGESS, Joshua , KHANNA, Anupama , SCHLINGMAN, Daniel , VAIDYANATHAN, Ramesh
IPC分类号： C12N9/22
CPC分类号： C12N9/1241 , C12N9/22 , C12N15/1068 , C12Q1/6869 , C12Q2521/507 , C12Q2565/514
摘要： Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.
摘要翻译：呈现在使用相同的转座酶和反应条件，以提高破碎和核酸样品的标记，特别是改变的转座酶和反应条件表现出改善的插入序列偏置，以及方法和试剂盒。

93. 发明授权

EP2426221B1 Recombinase polymerase amplification 有权转让
标题翻译：重组聚合酶扩增
公开(公告)号：EP2426221B1
公开(公告)日：2016-06-22
申请号：EP11170060.5
申请日：2007-05-04
申请人： Alere San Diego, Inc.
发明人： Armes, Niall, Antony , Piepenburg, Olaf , Parker, Mathew, James, David
IPC分类号： C12Q1/68 , C12N9/00
CPC分类号： C12N9/1241 , C12N9/00 , C12Q1/6844 , C12Y207/07 , C12Q2521/507 , C12Q2531/119

94. 发明公开

EP2989212A1 STRAND-INVASION BASED DNA AMPLIFICATION METHOD 有权转让
标题翻译： DNA扩增对列车BASIS侵袭
公开(公告)号：EP2989212A1
公开(公告)日：2016-03-02
申请号：EP14722601.3
申请日：2014-04-23
申请人： Orion Diagnostica Oy
发明人： FILÉN, Sanna
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6844 , C12Q2600/158 , C12Q2521/507 , C12Q2527/101 , C12Q2561/109
摘要： A method for detecting toxigenic Clostridium difficile (C. difficile) by strand-invasion based DNA amplification is provided, together with oligonucleotides, compositions and kits suitable for use in this method.

95. 发明授权

EP2569453B1 NUCLEIC ACID ISOLATION METHODS 有权
标题翻译：核酸分离方法
公开(公告)号：EP2569453B1
公开(公告)日：2015-12-16
申请号：EP11781412.9
申请日：2011-05-16
申请人： Fluidigm Corporation
发明人： MAY, Andrew , MIR, Alain , RAMAKRISHNAN, Ramesh , ZIMMERMANN, Bernhard
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12P19/34 , C07H21/00 , C12Q1/6806 , C12Q1/6869 , C12Q2600/118 , C12Q2565/113 , C12Q2527/125 , C12Q2525/204 , C12Q2525/307 , C12Q2525/15 , C12Q2521/507

96. 发明公开

EP2880182A1 RECOMBINASE MEDIATED TARGETED DNA ENRICHMENT FOR NEXT GENERATION SEQUENCING 有权
标题翻译： ABKERBINASEVERMITTELTE，ABGERICHTETE DNA-ANREICHERUNGFÜRSEQUENZIERUNG DERNÄCHSTENGENERATION
公开(公告)号：EP2880182A1
公开(公告)日：2015-06-10
申请号：EP13748003.4
申请日：2013-08-02
申请人： Qiagen GmbH
发明人： WEDLER, Holger , WEDLER, Erika
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6806 , C12Q1/6869 , C12Q1/6874 , C12Q2521/507 , C12Q2537/159 , C12Q2561/109
摘要： The present invention provides a method for enriching target sequences from a sequencing library to provide a target enriched sequencing library, wherein the sequencing library is suitable for massive parallel sequencing and comprises a plurality of double-stranded nucleic acid molecules, wherein the method comprises: a) providing nucleoprotein filaments comprising (i) a single stranded invasion probe, wherein the invasion probe has a region of substantial complementarity to one strand of a double-stranded target sequence, (ii) a recombinase; b) forming a complex between the invasion probe and a complementary portion of the target sequence wherein complex formation is mediated by the recombinase; c) separating the complexes from the remaining sequencing library, thereby enriching the target sequences. The present invention relies on the use of a recombinase such as Rec A for enriching target nucleic acid sequences from a sequencing library.
摘要翻译：本发明提供用于从测序文库富集靶序列以提供靶富集的测序文库的方法，试剂盒和组合物，其中测序文库适用于大规模的平行测序，并且包含多个双链核酸分子。

97. 发明公开

EP2829615A1 Kit for multiplexing recombinase polymerase amplification 有权转让
标题翻译： Kit zur Multiplexierung einer Rekombinase-Polymerase-Verstärkung
公开(公告)号：EP2829615A1
公开(公告)日：2015-01-28
申请号：EP14170595.4
申请日：2006-07-25
申请人： Alere San Diego, Inc.
发明人： Piepenburg, Olaf , Williams, Colin H. , Armes, Niall A.
IPC分类号： C12Q1/68
CPC分类号： C12N15/113 , C12Q1/6804 , C12Q1/6816 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , G01N21/78 , G01N33/558 , G01N2021/7759 , G01N2021/7763 , G01N2021/7793 , C12Q2521/507 , C12Q2565/102 , C12Q2537/1376 , C12Q2565/101 , C12Q2525/119 , C12Q2521/301 , C12Q2561/113 , C12Q2537/125 , C12Q2525/125 , C12Q2563/131 , C12Q2565/625
摘要： This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
摘要翻译：本公开提供了用于快速多重RPA反应的方法和试剂以及用于检测多重RPA反应产物的改进方法。此外，本公开提供了消除RPA过程之间的残留污染的新方法。

98. 发明公开

EP2811019A1 METHOD FOR CORRECTING DETECTION SIGNAL IN ISOTHERMAL NUCLEIC ACID AMPLIFICATION REACTION 审中-公开
标题翻译： VERFAHREN ZUR KORREKTUR EINES ERKENNUNGSSIGNALS IN EINER ISATHERENNUCLEINSÄUREAMPLIFIKATIONSREAKING
公开(公告)号：EP2811019A1
公开(公告)日：2014-12-10
申请号：EP13743774.5
申请日：2013-01-23
申请人： FUJIREBIO INC.
发明人： MATSUNO, Tatsuki
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6844 , C12Q2521/507 , C12Q2527/101 , C12Q2545/101 , C12Q2549/113
摘要： The present invention provides a method for stably correcting a detection signal in an isothermal nucleic acid amplification reaction, particularly an isothermal nucleic acid amplification reaction performed at low temperature.
Specifically, the present invention provides a method for detecting a target nucleic acid in an isothermal nucleic acid amplification reaction, comprising the following (1) and (2):
(1) subjecting a nucleic acid sample to an amplification reaction of a target nucleic acid under an isothermal condition in the presence of a nucleic acid probe for correction and a nucleic acid probe for detection of the target nucleic acid; and
(2) correcting a detection signal due to the nucleic acid probe for detection with a detection signal due to the nucleic acid probe for correction in the amplification reaction.
摘要翻译：本发明提供了在等温核酸扩增反应中稳定校正检测信号的方法，特别是在低温下进行的等温核酸扩增反应。具体地，本发明提供了在等温核酸扩增反应中检测靶核酸的方法，其包括以下（1）和（2）：（1）使核酸样品进行靶核酸的扩增反应在用于校正的核酸探针和用于检测靶核酸的核酸探针的存在下的等温条件下，和（2）由于用于在扩增反应中进行校正的核酸探针而用检测信号来校正由于用于检测的核酸探针的检测信号。

99. 发明授权

EP2290096B1 Recombinase polymerase amplification using a temperature-sensitive recombinase agent 有权转让
标题翻译： Rekombinase-Polymerase-Amplifikationsverfahren unter Verwendung eines temperaturempfindlichen Rekombinationsmittels
公开(公告)号：EP2290096B1
公开(公告)日：2014-11-19
申请号：EP10180775.8
申请日：2003-02-21
申请人： Alere San Diego, Inc.
发明人： Armes, Niall A. , Stemple, Derek L.
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6806 , C12Q1/6848 , C12Q1/686 , G06F17/30864 , G06F17/30902 , C12Q2521/507 , C12Q2522/101 , C12Q2549/119 , C12Q2527/101 , C12Q2531/119

100. 发明公开

EP2569453A2 NUCLEIC ACID ISOLATION METHODS 有权
标题翻译：核酸分离方法
公开(公告)号：EP2569453A2
公开(公告)日：2013-03-20
申请号：EP11781412.9
申请日：2011-05-16
申请人： Fluidigm Corporation
发明人： MAY, Andrew , MIR, Alain , RAMAKRISHNAN, Ramesh , ZIMMERMANN, Bernhard
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12P19/34 , C07H21/00 , C12Q1/6806 , C12Q1/6869 , C12Q2600/118 , C12Q2565/113 , C12Q2527/125 , C12Q2525/204 , C12Q2525/307 , C12Q2525/15 , C12Q2521/507
摘要： The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

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