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91. 发明公开

EP2358909A1 A SYSTEM USEFUL FOR REPORTING PROTEIN-PROTEIN INTERACTIONS IN THE BACTERIAL PERIPLASM 有权
标题翻译：系统来录制相互作用之间的蛋白质在细菌周质
公开(公告)号：EP2358909A1
公开(公告)日：2011-08-24
申请号：EP09826911.1
申请日：2009-11-16
申请人： Cornell University
发明人： DELISA, Matthew, P. , MANSELL, Thomas, J.
IPC分类号： C12Q1/68
CPC分类号： C12N15/1055 , C07K2319/034 , C07K2319/61 , C07K2319/70
摘要： One aspect of the present invention relates to a reporter system for detection of protein-protein interactions in the periplasm of a prokaryotic host cell. The reporter system includes a first expression system which has a nucleic acid molecule encoding a first fragment of a reporter protein molecule, a nucleic acid molecule encoding a first signal sequence, and a nucleic acid molecule encoding a first member of a putative binding pair, where the nucleic acid molecule encoding the first fragment, the nucleic acid molecule encoding the first signal sequence, and the nucleic acid molecule encoding the first member of the putative binding pair are operatively coupled to permit their expression in a prokaryotic host cell as a first fusion protein. The reporter sys tem also includes a second expression system which has a nucleic acid molecule encoding a second fragment of the reporter protein molecule, a nucleic acid molecule encoding a second signal sequence, and a nucleic acid molecule encoding a second member of the putative binding pair, where the nucleic acid molecule encoding the second fragment, the nucleic acid molecule encoding the second signal sequence, and the nucleic acid molecule encoding the second member of the putative binding pair are operatively coupled to permit their expression in a prokaryotic host cell as a second fusion protein, where, when expressed in a prokaryotic host cell, at least one of the first and the second fusion proteins are co-translationally transported to the periplasm where, when present, the first and second members of the putative binding pair bind together and the first and second fragments of the reporter protein molecule are reconstituted, thereby producing an active reporter protein. The reporter system may be used to carry out methods of identifying candidate compounds which bind to a target protein, identifying a candidate gene which modulates binding between a first protein and second protein, and identifying a candidate compound which modulates binding between a first protein and second protein.

92. 发明授权

EP1327003B1 MAMMALIAN TWO-HYBRID SYSTEM FOR SCREENING FOR MODULATORS OF THE ACCUMULATION OF METABOLIC PRODUCTS 有权
标题翻译：哺乳动物“双杂交”体系筛选代谢调节剂积累的产品
公开(公告)号：EP1327003B1
公开(公告)日：2011-08-17
申请号：EP01977707.7
申请日：2001-10-09
申请人： The Regents of The University of California
发明人： TETSU, Osamu , WAKITA, Kenichi , MCCORMICK, Frank
IPC分类号： C12Q1/68
CPC分类号： C12N15/1055 , A61K48/00 , A61K48/0058 , C12Q1/6897 , C12Q2565/201
摘要： This invention utilizes a two-hybrid system to screen for agents that modulate the ability of a cell to degrade or to accumulate a metabolic product or to selective kill a cell or to selectively express a gene or cDNA in a cell that has a defect in its ability to degrade or to accumulate a metabolic product. One embodiment provides a mammalian cell comprising a nucleic acid encoding a peptide binding domain and an effector gene; a first chimeric protein comprising a nucleic acid binding domain that binds the peptide binding domain attached to the metabolic product or to a ligand that binds to the metabolic product; and a second chimeric protein comprising an expression control protein attached to the metabolic product or to the ligand that binds to the metabolic product such that when the first chimeric protein comprises the metabolic product, the second chimeric protein comprises the ligand and when the first chimeric protein comprises the ligand, the second chimeric protein comprises the metabolic product. The cell is contacted with a test agent and an alteration of expression of the effector gene is detected (if present) where a change in expression of the effector gene in response to the test agent indicates that the test agent modulates the ability of the cell to accumulate or degrade the metabolic product.

93. 发明授权

EP1027608B1 PROTEIN FRAGMENT COMPLEMENTATION ASSAYS 有权
标题翻译：蛋白片段补充测定
公开(公告)号：EP1027608B1
公开(公告)日：2010-01-20
申请号：EP99936199.1
申请日：1999-07-30
申请人： Odyssey Thera, Inc.
发明人： MICHNICK, Stephen, William, Watson , PELLETIER, Joelle, Nina , REMY, Ingrid
IPC分类号： G01N33/58 , G01N33/566 , G01N33/68
CPC分类号： G01N33/566 , C12N15/1055 , G01N33/581 , G01N33/68 , G01N2500/00

94. 发明公开

EP1910550A4 MULTIPLE GENE EXPRESSION INCLUDING SORF CONSTRUCTS AND METHODS WITH POLYPROTEINS, PRO-PROTEINS, AND PROTEOLYSIS 审中-公开
标题翻译：表达的几个基因，OF SORF构建体及与多聚PROPROTEINEN和蛋白水解方法，包括
公开(公告)号：EP1910550A4
公开(公告)日：2009-11-04
申请号：EP06788320
申请日：2006-07-21
申请人： ABBOTT LAB
发明人： CARSON GERALD R , GION WENDY , SALFELD JOCHEN G , GU JIJIE , REGIER DEAN A , KUNES YUNE
IPC分类号： C12N15/63 , C12P21/06 , C07H21/02 , C07H21/04 , C07K16/18 , C12N1/18 , C12N5/07 , C12N5/071 , C12N15/00 , C12P21/08
CPC分类号： C12N15/67 , C07K16/00 , C07K2319/50 , C07K2319/92 , C12N15/1055 , C12P21/02 , C12P21/06

95. 发明授权

EP1807697B1 SYSTÈME DOUBLE-HYBRIDE BASÉ SUR LA MISE SOUS SILENCE DE GÈNES PAR INTERFÉRENCE TRANSCRIPTIONNELLE. 有权
标题翻译：基于基因沉默BY转录干扰双杂交系统
公开(公告)号：EP1807697B1
公开(公告)日：2009-10-21
申请号：EP05812049.4
申请日：2005-10-24
申请人： Aventis Pharma S.A.
发明人： FOURNIER, Alain , BEHRENS, Ralf , BANDIERA, Silvio
IPC分类号： G01N33/53 , C12N15/65 , C12N15/81 , C12Q1/68
CPC分类号： C12N15/1055
摘要： The invention relates to a novel double hybrid system and to the uses thereof. This system provides, in particular, a tool that enables the detection of the interruption of a protein-protein interaction. The developed system uses transcription interference as a mechanism for detecting a breaking up of interacting protein pairs. The developed double hybrid system can be applied to the screening of molecules enabling the detection of molecules breaking up a protein-protein interaction as well as enabling the identification of alleles lacking in the interaction of proteins involved in the protein-protein interactions.

96. 发明公开

EP2002021A4 METHODS FOR ASSAYING PROTEIN-PROTEIN INTERACTION 有权转让
标题翻译：程序对蛋白质 - 蛋白质相互作用
公开(公告)号：EP2002021A4
公开(公告)日：2009-04-22
申请号：EP07758759
申请日：2007-03-16
申请人： INVITROGEN CORP
发明人： LEE KEVIN J , POLLOK BRIAN A , ZHONG ZHONG
IPC分类号： G01N33/50 , C12N15/10 , C12Q1/37 , C12Q1/68 , G01N33/542
CPC分类号： G01N33/542 , C07K2319/50 , C07K2319/71 , C07K2319/80 , C12N15/1055 , G01N2500/10
摘要： The invention relates, in part, to methods for detecting, monitoring, measuring or assessing an interaction between at least two proteins. The invention also relates, in part, to methods for determining if a test compound, or a mix of compounds, modulates an interaction between at least two proteins. In some embodiments, determination is made possible via the use of two recombinant molecules, e.g., one of which contains a first protein cleavage site for a proteolytic molecules, and an activator of a gene. A second recombinant molecule may include a second protein and the proteolytic molecule. Various other formats are provided by the invention. In some embodiments, if the test compound binds to the first protein, a reaction is initiated whereby the activator is cleaved, and activates a reporter gene.

97. 发明授权

EP1913140B1 VERFAHREN FÜR DIE KONTINUIERLICHE ZIELGERICHTETE EVOLUTION VON PROTEINEN IN VITRO 有权
标题翻译：过程在蛋白质的不断发展目标锁定在体外
公开(公告)号：EP1913140B1
公开(公告)日：2008-12-24
申请号：EP06763003.8
申请日：2006-08-07
申请人： GENEART AG
发明人： LISS, Michael
IPC分类号： C12N15/10 , C40B10/00
CPC分类号： C12N15/1058 , C12N15/1055 , C12N15/1075 , C40B10/00
摘要： The invention relates to the production of variants of a protein in an in vitro evolution method, comprising the steps: (A) preparing an in vitro expression system, comprising: (i) a nucleic acid sequence S, which codes for a protein Y to be varied; (ii) a target molecule X 1 capable of binding to protein Y and/or at least one variant Y thereof; (iii) an RNA polymerase (Pol) capable of transcribing the nucleic acid sequence S; (iv) a reverse transcriptase (RT) capable of reverse transcribing transcripts of nucleic acid sequence S, the target molecule X being coupled to Pol and protein Y to RT, or target molecule X being coupled to RT and protein Y being coupled to Pol; (B) incubating the in vitro expression system from (A) under conditions that enable a transcription, a reverse transcription and a translation while forming variants Y' of protein Y and nucleic acid sequences S' coding therefor, and which promote the formation of variants Y' with improved binding properties for target molecule X; (C) isolating and optionally characterizing those variants Y' that have improved binding properties for binding to X and/or isolation nucleic acid sequence variants S' that code for Y'.

98. 发明公开

EP1917347A4 COMPOSITIONS AND METHODS FOR ANALYZING PROTEIN INTERACTIONS 有权
标题翻译：组合物和方法蛋白质相互作用分析
公开(公告)号：EP1917347A4
公开(公告)日：2008-11-12
申请号：EP06789929
申请日：2006-08-22
申请人： CORNELL RES FOUNDATION INC , RES DEV FOUNDATION
发明人： DELISA MATTHEW , GEORGIOU GEORGE , WARAHO DUJDUAN
IPC分类号： G01N33/50 , C12N1/20 , C12N15/10 , C12P21/04 , C12P21/06
CPC分类号： C12N15/1055 , C40B30/04 , G01N33/5032

99. 发明公开

EP1913139A1 VERFAHREN FÜR DIE KONTINUIERLICHE ZIELGERICHTETE EVOLUTION VON PROTEINEN IN VIVO 有权
标题翻译：过程在蛋白质的不断发展目标锁定在体内
公开(公告)号：EP1913139A1
公开(公告)日：2008-04-23
申请号：EP06763002.0
申请日：2006-08-07
申请人： GENEART AG
发明人： LISS, Michael
IPC分类号： C12N15/10 , C40B10/00
CPC分类号： C12N15/1058 , C12N15/1055 , C40B10/00
摘要： The invention relates to methods for producing variants of proteins that, in comparison to the starting protein, have improved properties. These methods are obtained with the aid of an in vivo evolution method.

100. 发明公开

EP1877583A2 SEQUENCE ENABLED REASSEMBLY (SEER) - A NOVEL METHOD FOR VISUALIZING SPECIFIC DNA SEQUENCES 审中-公开
标题翻译： SEQUENCE激活重排的一种新方法可视化特定DNA序列
公开(公告)号：EP1877583A2
公开(公告)日：2008-01-16
申请号：EP06770039.3
申请日：2006-05-05
申请人： Arizona Board of Regents on behalf of the Unversity of Arizona , THE SCRIPPS RESEARCH INSTITUTE
发明人： SEGAL, David, J , GHOSH, Indraneel , OOI, Aik,T , PORTER, Jason , STAINS, Cliff, I , BARBAS, Carlos,F
IPC分类号： C12Q1/68
CPC分类号： C12N15/1086 , C12N15/1055 , G01N33/574
摘要： The present invention provides a nucleotide sequence detection system in which a reporter enzyme is split into two halves each half of which is associated with at least one zinc finger domain. Upon DNA binding to the specific sequence defined by the zinc finger domains associated with the respective halves, the split-protein reassembles to reconstitute a functional enzyme. As such, the present invention provides methods of using the nucleotide sequence detection system for various diagnostic and identification purposes.

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